Zombie Red™ Fixable Viability Kit from BioLegend

Product Specs
ItemZombie Red™ Fixable Viability Kit
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number423109
Quantity100T
ApplicationsFC,ICFC,ICC

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Receptor binding domain-independent pancoronavirus vaccine design by fusion of conserved T/B EpitopesEmerging Microbes & InfectionsDecember 31, 2026Yunru Yang, Yetian Chen, Mengyu Hong, Ronghua Zou, Jingxue Yao, Entao Li, Jiayi Wang, Xiaodong Ye, Yixiang Xing, Yangming Tang, et al.(SP6 and SP7)) for 24 h (37°C, 5% CO2). Cells were stained with Zombie Red™ viability dye (BioLegend, 423109) and the following antibody cocktail (1 h, ice, dark): anti-CD4-APC/Cy7 (300518), anti-CD8-PE/Cy7 (344750),

(2) Pharmacological Inhibition of Small Extracellular Vesicle Secretion by ALK5i SD-208 via Lysosomal Rerouting of CD63+ CompartmentsJ Extracell VesiclesAugust 29, 2026Rahul Sanwlani, Georgina H. Thompson, Kyle Bramich, Ching-Seng Ang, Millie L. Trowbridge, Chris E. Duringer, Vlad Stolojan, Sean M. Davidson, Konstantinos Savvatis, John H. McVey, et al.viability staining, one sample was incubated with Zombie Red fixable viability dye (1:100; BioLegend, 423109) for 30 min in the dark. For all other samples, 500 µL of Cell Staining Buffer (BioLegend, 420201) was

(3) Ptpn2 limits plasma cell fate and antiviral immunity by integrating B cell receptor and IFN-γ signals in B cellsSci AdvAugust 21, 2026Ana Maria Hincapie, Alexandre Poirier, Isabelle Aubry, Philippe Aumont, Aanya Bhagrath, Noriko Uetani, Bianca Colalillo, Chenyue Wu, Benoit Charbonneau, Stephanie Bussieres-Marmen, et al.then analyzed via flow cytometry after cell surface and viability staining with Zombie Red (cat. no. 423109, BioLegend) following the manufacturer’s instructions. In vitro class-switch recombination Isolated

(4) Adipocyte-derived LTB4 programs human NKG2A+ γδ T cells as cytotoxic sentinels at the adipose-tumor interface in breast cancerJ Immunother CancerJuly 28, 2026Xiaoxiao Hu, Dang Wu, Fang Jia, Sangsang Zhu, Shouyu Ke, Di Chen, Jing Zhao, Lili Li, Yi Zhang, Zhen Wang, et al.Flow cytometry analysis Dead cell exclusion was performed using the fixable viability stain Zombie Red (BioLegend, Cat# 423109). To stain cell surface antigens, single-cell suspensions were stained in FACS buffer, PBS with 0.5%

(5) Increased Nur77 is disconnected from TCR affinity in insulin-specific TregsJ ImmunolJuly 2, 2026Yi Jing, Yuelin Kong, Baoyu Liu, Elizabeth Kolawole, Tessa Galland, Luke G Gillen, Maren Kenison, Maran Sprouse, Brian D Evavold, Matthew L Bettini, et al.TruStain FcX reagent (BioLegend; 101320). Viable cells were identified using Zombie Dyes (BioLegend; 423109&423107). For intracellular staining, cells were fixed using the eBioscience Foxp3/Transcription Factor

(6) Engineered oncolytic virus armed with anti-PCSK9 scFv boosts long-term CD8+ T cell immunity via rewiring MHC-I antigen presentationCell Reports MedicineApril 21, 2026Huolun Feng, Yuhan Zhang, Zuda Huang, Jianlong Zhou, Yongfeng Liu, Xiao Xiao, Mingxi Chen, Xin Guo, Jiabin Zheng, Zejian Lyu, et al.Convertase 9/PCSK9 ELISA Kit,LiankeBio,Cat# EK1124 Zombie Redᵀᴹ Fixable Viability Kit,BioLegend,Cat# 423109 Color Reverse Transcription Kit,EZBioscience,Cat# A0010CGQ EasySep Mouse T Cell Isolation Kit,STEMCELL Technologies

(7) Comprehensive Analysis of Neutrophil Immunomodulatory Properties and FcR Dynamics in HIV-1 Infection and TherapyiScienceApril 17, 2026Soledad Marsile-Medun, Manon Souchard, Daouda Abba Abba Moussa, Valérie Lorin, Hugo Mouquet, Elisa Reynaud, Rayane Dibsy, Edouard Tuaillon, Delphine Muriaux, Giang Ngo, et al.phenotypic activation, cells were stained at room temperature with a viability dye (ZombieRed™, Biolegend #423109) for 15 minutes then washed and stained for 20 minutes at room temperature with fluorochrome-conjugated

(8) PTPN1/PTPN2 inhibition improves NK cancer therapy by enhancing IL-2 and mitigating TGFβ1 responsesEMBO ReportsApril 15, 2026Chu-Han Feng, Linda Peltier, Tiffanie Chouleur, Milea DiPonzio, Isabelle Aubry, Alexandre J Poirier, Zuzet M Cordova, Yunyun Shen, Sébastien Tabariès, Xiaona Cao, et al.(Tumor infiltrated cells)",BioLegend,329929 "Fixable Viability Dye – Zombie Red, 1:1000",BioLegend,423109 "Fixable Viability Dye – eFluor 780, 1:2000",Invitrogen,65-0865-14 Western Blot Antibodies,Source,Catalog

(9) Bipolar CD4-targeted dual-DARPin-55/57 lipid nanoparticle enables efficient CRISPR/Cas-mediated HIV-1 DNA excision and reactivation blockade in latent CD4 T cell linesMaterials Today BioApril 1, 2026Subhra Mandal, Abdul Rasheed Baloch, Xinxu Yuan, Jackson Chen, A. Sami Saribas, Yuanjun Zhu, Danmeng Zhang, Dabbu Jaijyan, Jian Xu, Reafa Hossain, et al.(monoclonal),"Mouse IgG1, κ",BioLegend,302216 Viability (Live/Dead),Zombie Red™ (Fixable viability dye),,,BioLegend,423109 Fluorochrome-conjugated antibodies used for PBMC staining.

(10) Liver-directed AAV-IL-10 therapy enhances CD8+ T cell-mediated immunity against hepatocellular carcinomaJ Immunother CancerMarch 27, 2026Chia-I Lin, Yi-Wei Chiu, Meng-Syuan Yen, Yu-Wen Wang, Ying-Hsuan Wu, Ya-Hui ChuangZombie UV Fixable Viability Kit,UV,BioLegend,Cat# 423107 Zombie Red Fixable Viability Kit,PE/Dazzle594,BioLegend,Cat# 423109 List of monoclone antibodies for flow cytometry APC, Allophycocyanin; mAbs, monoclone antibodies;

(11) Chromobox 2 inhibition: A novel activity of alisertib, an Aurora A kinase inhibitorMol Cancer TherJanuary 24, 2026Tomomi M. Yamamoto, Ritsuko Iwanaga, Elizabeth R. Woodruff, Alan M. Elder, Alexander Petkov, Elmar Nurmemmedov, Elan Eisenmesser, Philip Reigan, Benjamin G. Bitler, Lindsay W. Brubakerwere washed with cold HBSS buffer (2% FBS/HBSS). Cells were stained with Zombie Red (BioLegend, Cat.# 423109) for viability. ALDH activity was analyzed using Novocyte Penteon (Agilent). Cytometry data were analyzed

(12) Chromobox 2 inhibition: A novel activity of alisertib, an Aurora A kinase inhibitorMolecular Cancer TherapeuticsJanuary 24, 2026Tomomi M. Yamamoto, Ritsuko Iwanaga, Elizabeth R. Woodruff, Alan M. Elder, Alexander Petkov, Elmar Nurmemmedov, Elan EISENMESSER, Philip Reigan, Benjamin G. Bitler, Lindsay W. Brubakerwere washed with cold HBSS buffer (2% FBS/HBSS). Cells were stained with Zombie Red (BioLegend, Cat.# 423109) for viability. ALDH activity was analyzed using Novocyte Penteon (Agilent). Cytometry data were analyzed

(13) Physical frailty in relation to immunosenescence involving pDC function in the Japanese elderly: a cross-sectional survey studyImmunity & AgeingJanuary 19, 2026Yoshihiko Sugihara, Yusuke Ushida, Yuko Fukushima, Hajime Nozawa, Ryohei Tsuji, Daisuke Fujiwara, Ayuka Kawakami, Kouki Tomida, Hiroyuki Shimada, Mitsuo Maruyamain 1.5-mL tubes and washed with PBS. Cells were stained with the Zombie Red Fixable Viability Kit (423109, BioLegend) in PBS for 15 min at 4℃ and then washed with FACS buffer (PBS supplemented with 0.5% bovine

(14) Alpha-gal–specific human IgE promotes both alpha-gal–induced and antigen-independent allergic effector cell activationJournal of Allergy and Clinical ImmunologyJanuary 12, 2026Yinghui Wang, Yugen Zhang, Christa Dudley, Katherine Fitzgerald, Audrey S. Carson, Camille M. Kapita, Daaniya Rana, Kim Kluckman, Donna Bortner, Dale O. Cowley, et al.cells with FACS buffer (DPBS + 2% FBS + 2mM EDTA), a mixture of Zombie-red (1:1000, Biolegend, cat#423109), BV421-anti-human FcεRI (1:300, Biolegend, clone#AER-37), and AF647-anti-human IgE (1:200, Biolegend,

(15) Apoptotic metabolites synthesize and inherit unique de novo L2a/L2b RNAs to prevent virus infectionBioact MaterDecember 10, 2025Peiyi Li, Yelin Lan, Xutong Yan, Zeyuan Cao, Jingyun Ji, Mingqiang Deng, Dongmei He, Ruoxin Huang, Yan Qu, Yaoxing Wu, et al.as described above. At 4 h of apoptosis induction, the TF3-DEVD-FMK (1:150) and Zombie Red (1:150) (#423109, Biolegend) were added into the culture medium, respectively. After 2 h incubation in 37 °C, the MSCs

(16) Targeting an essential viral oncoprotein with an IL-7-enhanced mRNA vaccine induces durable immunity to Merkel cell carcinomaCell ReportsOctober 28, 2025Alexander Frey, Kathryn Clulo, Yuewei Fei, Therese Cordero Dumit, Frankie Scallo, Jerry William Williams, Emily Chang, Curtis J. Perry, Lena V. Wirth, Daniel Jacobs, et al.stained with TotalSeq anti-human hashtags C0251- C0258 (Biolegend), viability dye (zombie red, Biolegend 423109) and anti-human CD45-FITC (clone HI30, Biolegend 304038) and enriched for live cells and up to 50% immune

(17) AI-Validated Brain Targeted mRNA Lipid Nanoparticles with Neuronal TropismACS NanoOctober 21, 2025Mor Sela, Gal Chen, Haim Kadosh, Tomer Kagan, Raneen Nicola, Sally Turutov, Yuval Richtman, Lin Zhige, Mia R. Albalak Menasherov, Shaked Kagan, et al.ice in the dark. Subsequently, cells were washed once with PBS and resuspended with Zombie Red (BLG-423109; BioLegend, US) regent according to the manufacturer protocol. Cells were incubated with the reagent

(18) Coronin 1 deficiency protects from the development of autoimmune myocarditis by reducing CD4+ T cellsESC Heart FailureOctober 1, 2025Amanda Ochoa‐Espinosa, Lucile Genty, Lifen Xu, Asli Akin, Katharina Glatz, Sarah Decembrini, Christian Mueller, Albert Neutzner, Rajesh Jayachandran, Felix Mahfoud, et al.(Biolegend #100713), anti‐CD49B‐APC (Biolegend #128016) and Zombie Red fixable viability dye (Biolegend # 423109). Mix2: anti‐CD45‐PerCP (Biolegend #103129), anti‐CD3‐BV605 (Biolegend #100351), anti‐CD19‐BV605 (Biolegend

(19) Tumor-Derived CCL16 Normalizes Tumor Vasculature through Macrophage ICAM-1 Receptor and Enhances Immunotherapy Efficacy in Hepatocellular CarcinomaCancer ResearchOctober 1, 2025Kunling Chen, Huolun Feng, Yujie Zhang, Jingyuan Pei, Yuyan Xu, Xiangxu Wei, Zhuohao Chen, Zhoubin Feng, Lei Cai, Yong Li, et al.strainer and incubated with Fc block. Cells were first stained with Zombie Red Fixable Viability Dye (423109, BioLegend) to exclude dead cells. For surface staining, the cells were incubated with the following

(20) NNMT inhibition in cancer-associated fibroblasts restores antitumour immunityNatureSeptember 25, 2025Janna Heide, Agnes J. Bilecz, Samarjit Patnaik, Maria Francesca Allega, Leonhard Donle, Kaiting Yang, Ethan Teich, Yan Li, Qiaoshan Lin, Ke Kong, et al.staining: Samples were washed with PBS and incubated with Zombie NIR (BioLegend, 423105), Zombie Red (BioLegend, 423109), or Zombie Aqua (BioLegend, 423101) fixable viability dye (1:20) for 10 min at 4°C. Fc blocking was

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Zombie Red™ Fixable Viability Kit from BioLegend

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Zombie Red™ Fixable Viability Kit from BioLegend

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