Zombie Red™ Fixable Viability Kit from BioLegend

Product Specs
ItemZombie Red™ Fixable Viability Kit
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number423109
Quantity100 tests
ApplicationsFC - Quality tested, ICFC, ICC - Verified

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Receptor binding domain-independent pancoronavirus vaccine design by fusion of conserved T/B EpitopesEmerging Microbes & InfectionsDecember 31, 2026Yunru Yang, Yetian Chen, Mengyu Hong, Ronghua Zou, Jingxue Yao, Entao Li, Jiayi Wang, Xiaodong Ye, Yixiang Xing, Yangming Tang, et al.(SP6 and SP7)) for 24 h (37°C, 5% CO2). Cells were stained with Zombie Red™ viability dye (BioLegend, 423109) and the following antibody cocktail (1 h, ice, dark): anti-CD4-APC/Cy7 (300518), anti-CD8-PE/Cy7 (344750),

(2) Engineered oncolytic virus armed with anti-PCSK9 scFv boosts long-term CD8+ T cell immunity via rewiring MHC-I antigen presentationCell Reports MedicineApril 21, 2026Huolun Feng, Yuhan Zhang, Zuda Huang, Jianlong Zhou, Yongfeng Liu, Xiao Xiao, Mingxi Chen, Xin Guo, Jiabin Zheng, Zejian Lyu, et al.Convertase 9/PCSK9 ELISA Kit,LiankeBio,Cat# EK1124 Zombie Redᵀᴹ Fixable Viability Kit,BioLegend,Cat# 423109 Color Reverse Transcription Kit,EZBioscience,Cat# A0010CGQ EasySep Mouse T Cell Isolation Kit,STEMCELL Technologies

(3) Comprehensive Analysis of Neutrophil Immunomodulatory Properties and FcR Dynamics in HIV-1 Infection and TherapyiScienceApril 17, 2026Soledad Marsile-Medun, Manon Souchard, Daouda Abba Abba Moussa, Valérie Lorin, Hugo Mouquet, Elisa Reynaud, Rayane Dibsy, Edouard Tuaillon, Delphine Muriaux, Giang Ngo, et al.phenotypic activation, cells were stained at room temperature with a viability dye (ZombieRed™, Biolegend #423109) for 15 minutes then washed and stained for 20 minutes at room temperature with fluorochrome-conjugated

(4) PTPN1/PTPN2 inhibition improves NK cancer therapy by enhancing IL-2 and mitigating TGFβ1 responsesEMBO ReportsApril 15, 2026Chu-Han Feng, Linda Peltier, Tiffanie Chouleur, Milea DiPonzio, Isabelle Aubry, Alexandre J Poirier, Zuzet M Cordova, Yunyun Shen, Sébastien Tabariès, Xiaona Cao, et al.(Tumor infiltrated cells)",BioLegend,329929 "Fixable Viability Dye – Zombie Red, 1:1000",BioLegend,423109 "Fixable Viability Dye – eFluor 780, 1:2000",Invitrogen,65-0865-14 Western Blot Antibodies,Source,Catalog

(5) Bipolar CD4-targeted dual-DARPin-55/57 lipid nanoparticle enables efficient CRISPR/Cas-mediated HIV-1 DNA excision and reactivation blockade in latent CD4 T cell linesMaterials Today BioApril 1, 2026Subhra Mandal, Abdul Rasheed Baloch, Xinxu Yuan, Jackson Chen, A. Sami Saribas, Yuanjun Zhu, Danmeng Zhang, Dabbu Jaijyan, Jian Xu, Reafa Hossain, et al.(monoclonal),"Mouse IgG1, κ",BioLegend,302216 Viability (Live/Dead),Zombie Red™ (Fixable viability dye),,,BioLegend,423109 Fluorochrome-conjugated antibodies used for PBMC staining.

(6) Liver-directed AAV-IL-10 therapy enhances CD8+ T cell-mediated immunity against hepatocellular carcinomaJ Immunother CancerMarch 27, 2026Chia-I Lin, Yi-Wei Chiu, Meng-Syuan Yen, Yu-Wen Wang, Ying-Hsuan Wu, Ya-Hui ChuangZombie UV Fixable Viability Kit,UV,BioLegend,Cat# 423107 Zombie Red Fixable Viability Kit,PE/Dazzle594,BioLegend,Cat# 423109 List of monoclone antibodies for flow cytometry APC, Allophycocyanin; mAbs, monoclone antibodies;

(7) Chromobox 2 inhibition: A novel activity of alisertib, an Aurora A kinase inhibitorMolecular Cancer TherapeuticsJanuary 24, 2026Tomomi M. Yamamoto, Ritsuko Iwanaga, Elizabeth R. Woodruff, Alan M. Elder, Alexander Petkov, Elmar Nurmemmedov, Elan EISENMESSER, Philip Reigan, Benjamin G. Bitler, Lindsay W. Brubakerwere washed with cold HBSS buffer (2% FBS/HBSS). Cells were stained with Zombie Red (BioLegend, Cat.# 423109) for viability. ALDH activity was analyzed using Novocyte Penteon (Agilent). Cytometry data were analyzed

(8) Chromobox 2 inhibition: A novel activity of alisertib, an Aurora A kinase inhibitorMol Cancer TherJanuary 24, 2026Tomomi M. Yamamoto, Ritsuko Iwanaga, Elizabeth R. Woodruff, Alan M. Elder, Alexander Petkov, Elmar Nurmemmedov, Elan Eisenmesser, Philip Reigan, Benjamin G. Bitler, Lindsay W. Brubakerwere washed with cold HBSS buffer (2% FBS/HBSS). Cells were stained with Zombie Red (BioLegend, Cat.# 423109) for viability. ALDH activity was analyzed using Novocyte Penteon (Agilent). Cytometry data were analyzed

(9) Physical frailty in relation to immunosenescence involving pDC function in the Japanese elderly: a cross-sectional survey studyImmunity & AgeingJanuary 19, 2026Yoshihiko Sugihara, Yusuke Ushida, Yuko Fukushima, Hajime Nozawa, Ryohei Tsuji, Daisuke Fujiwara, Ayuka Kawakami, Kouki Tomida, Hiroyuki Shimada, Mitsuo Maruyamain 1.5-mL tubes and washed with PBS. Cells were stained with the Zombie Red Fixable Viability Kit (423109, BioLegend) in PBS for 15 min at 4℃ and then washed with FACS buffer (PBS supplemented with 0.5% bovine

(10) Alpha-gal–specific human IgE promotes both alpha-gal–induced and antigen-independent allergic effector cell activationJournal of Allergy and Clinical ImmunologyJanuary 12, 2026Yinghui Wang, Yugen Zhang, Christa Dudley, Katherine Fitzgerald, Audrey S. Carson, Camille M. Kapita, Daaniya Rana, Kim Kluckman, Donna Bortner, Dale O. Cowley, et al.cells with FACS buffer (DPBS + 2% FBS + 2mM EDTA), a mixture of Zombie-red (1:1000, Biolegend, cat#423109), BV421-anti-human FcεRI (1:300, Biolegend, clone#AER-37), and AF647-anti-human IgE (1:200, Biolegend,

(11) Apoptotic metabolites synthesize and inherit unique de novo L2a/L2b RNAs to prevent virus infectionBioact MaterDecember 10, 2025Peiyi Li, Yelin Lan, Xutong Yan, Zeyuan Cao, Jingyun Ji, Mingqiang Deng, Dongmei He, Ruoxin Huang, Yan Qu, Yaoxing Wu, et al.as described above. At 4 h of apoptosis induction, the TF3-DEVD-FMK (1:150) and Zombie Red (1:150) (#423109, Biolegend) were added into the culture medium, respectively. After 2 h incubation in 37 °C, the MSCs

(12) Targeting an essential viral oncoprotein with an IL-7-enhanced mRNA vaccine induces durable immunity to Merkel cell carcinomaCell ReportsOctober 28, 2025Alexander Frey, Kathryn Clulo, Yuewei Fei, Therese Cordero Dumit, Frankie Scallo, Jerry William Williams, Emily Chang, Curtis J. Perry, Lena V. Wirth, Daniel Jacobs, et al.stained with TotalSeq anti-human hashtags C0251- C0258 (Biolegend), viability dye (zombie red, Biolegend 423109) and anti-human CD45-FITC (clone HI30, Biolegend 304038) and enriched for live cells and up to 50% immune

(13) AI-Validated Brain Targeted mRNA Lipid Nanoparticles with Neuronal TropismACS NanoOctober 21, 2025Mor Sela, Gal Chen, Haim Kadosh, Tomer Kagan, Raneen Nicola, Sally Turutov, Yuval Richtman, Lin Zhige, Mia R. Albalak Menasherov, Shaked Kagan, et al.ice in the dark. Subsequently, cells were washed once with PBS and resuspended with Zombie Red (BLG-423109; BioLegend, US) regent according to the manufacturer protocol. Cells were incubated with the reagent

(14) Tumor-Derived CCL16 Normalizes Tumor Vasculature through Macrophage ICAM-1 Receptor and Enhances Immunotherapy Efficacy in Hepatocellular CarcinomaCancer ResearchOctober 1, 2025Kunling Chen, Huolun Feng, Yujie Zhang, Jingyuan Pei, Yuyan Xu, Xiangxu Wei, Zhuohao Chen, Zhoubin Feng, Lei Cai, Yong Li, et al.strainer and incubated with Fc block. Cells were first stained with Zombie Red Fixable Viability Dye (423109, BioLegend) to exclude dead cells. For surface staining, the cells were incubated with the following

(15) Coronin 1 deficiency protects from the development of autoimmune myocarditis by reducing CD4+ T cellsESC Heart FailureOctober 1, 2025Amanda Ochoa‐Espinosa, Lucile Genty, Lifen Xu, Asli Akin, Katharina Glatz, Sarah Decembrini, Christian Mueller, Albert Neutzner, Rajesh Jayachandran, Felix Mahfoud, et al.(Biolegend #100713), anti‐CD49B‐APC (Biolegend #128016) and Zombie Red fixable viability dye (Biolegend # 423109). Mix2: anti‐CD45‐PerCP (Biolegend #103129), anti‐CD3‐BV605 (Biolegend #100351), anti‐CD19‐BV605 (Biolegend

(16) Mechanistic Insights into Proglumide’s Role in Immune Cell Efficacy and Response to Immune Checkpoint Inhibitor Therapy in Hepatocellular CarcinomaCancers (Basel)September 14, 2025Priyanka S. Doneparthi, Hong Cao, Wenqiang Chen, Wenyu Dou, Hong-Bin Fang, Jill. P. Smitha pre-diluted Zombie RedTM fixable viability solution (100 μL) (BioLegend, San Diego, CA, USA, Cat# 423109). One million viable lymphocytes were added to 5 mL Falcon clear tubes (Fisher Scientific, Emeryville,

(17) Lrrk2 G2019S mutation incites increased cell-intrinsic neutrophil effector functions and intestinal inflammation in a model of infectious colitisNPJ Parkinsons DisAugust 29, 2025Jessica Pei, Nathalia L. Oliveira, Sherilyn J. Recinto, Alexandra Kazanova, Celso M. Queiroz-Junior, Ziyi Li, Katalina Couto, Susan Westfall, Ahmed M. Fahmy, Camila Tiefensee-Ribeiro, et al.Flow cytometry Following isolation, cells were stained with 1:2000 of Zombie red fixable viability dye (Biolegend, Cat# 423109) in PBS for 20 min on ice. Next, Fc-Block (anti-mouse CD16/CD32) 1:100 (Invitrogen, Cat# 14-01061-86)

(18) The transcription factor BATF pioneers the differentiation program of effector CD8+ T cells through the interaction with IRF4Cell ReportsAugust 26, 2025Sotaro Fujisawa, Yamato Tanabe, Arisa Hojo, Ryotaro Shiga, Junko Kurachi, Miki Koura, Toshikatsu Tamai, Yusuke Miyanari, E. John Wherry, Makoto KurachiViability Dye eFluor 780,eBioscience,Cat# 65-0865-14 Zombie Red Fixable Viability Kit,Biolegend,Cat# 423109 Brilliant Stain Buffer Plus,BD Biosciences,Cat# 566385 CellBlox Blocking Buffer,Invitrogen,Cat# B001T06F01 Recombinant human IL-2 protein

(19) Tumor-Derived CCL16 Normalizes Tumor Vasculature through Macrophage ICAM-1 Receptor and Enhances Immunotherapy Efficacy in Hepatocellular CarcinomaCancer ResAugust 12, 2025Kunling Chen, Huolun Feng, Yujie Zhang, Jingyuan Pei, Yuyan Xu, Xiangxu Wei, Zhuohao Chen, Zhoubin Feng, Lei Cai, Yong Li, et al.strainer and incubated with Fc block. Cells were first stained with Zombie Red Fixable Viability Dye (423109, BioLegend) to exclude dead cells. For surface staining, the cells were incubated with the following

(20) Long-Lived Immunogenic Cell Death Induced by a Water-Soluble Redox Active Au(I) Bis-N-Heterocyclic CarbeneJournal of the American Chemical SocietyJuly 9, 2025Matthew S. Levine, Sajal Sen, Esther Y. Maier, Mireya Mota, Miae Won, Jiexi Li, Jonathan F. Arambula, Vincent M. Lynch, Jong Seung Kim, Ronald A. DePinho, et al.treated with 1 μM of compounds 1–4 or oxaliplatin. Cells were stained with Zombie Red dye (BioLegend, 423109) as a viability stain followed by staining with anti-CRT polyclonal antibody. Cells were then fixed

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Zombie Red™ Fixable Viability Kit from BioLegend

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Zombie Red™ Fixable Viability Kit from BioLegend

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