Zombie Violet™ Fixable Viability Kit 423114 from BioLegend

Product Specs
ItemZombie Violet™ Fixable Viability Kit
CompanyBioLegend
Price $293.00Supplier Page View Company Product Page
Catalog Number423114
Quantity500T
ApplicationsFC,ICFC,ICC

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Glutamine transporter SLC1A5 inhibits autophagy-mediated CD276 degradation to promote esophageal cancer progressionCancer Biology & TherapyDecember 31, 2026Chunyan Wang, Hongyan Zhang, Chaonan Guan, Yuying Li, Shengli Yang, Lan Huangstaining for CD276: Cells were washed with FACS buffer (PBS + 2% FBS), stained with Zombie Violet (#423113, Biolegend) for 10 min at RT for live/dead exclusion (gated on Zombie Violet- cells). Then cells were

(2) Safety and immunogenicity of an adjuvant-free peptide vaccine targeting IL-17A: Phase 1 randomized controlled trialiScienceAugust 21, 2026Satoru Kasahara, Akiko Tenma, Makoto Sakaguchi, Shigeyoshi Tsuji, Hideki Tomioka, Ryuichi Morishita, Hironori Nakagami, Tetsuya TomitaSulfuric acid,Sigma-Aldrich Co. LLC.,Cat# 285940-5 ZOMBIE Violet Fixable Viability Kit,BioLegend,Cat# 423113 Cell Trace CFSE Cell Proliferation Kit,Thermo Fisher Scientific,Cat# C34554 Anti-Aggregate Wash (20×),CTL,Cat#

(3) Mitochondrial profiling across macrophage states reveals inhibition of IL-4/IL-13 reprogramming by the integrated stress responseSci AdvAugust 21, 2026Joan Blanco-Fernandez, Miriam Lisci, Mads M. Foged, Chloé Chapuis, Tim Pflästerer, Tatjana Kleele, Alexis A. JourdainFlow cytometry BMDM were stained with Zombie fixable viability NIR (BioLegend, 423105) or Violet (BioLegend, 423113) in PBS for 20 min at 4°C and washed in PBS. Cell surface staining was performed in fluorescence-activated cell sorting

(4) Köhlmeier-Degos disease is an interferonopathy characterized by type I and II interferon-driven inflammatory vasculopathyCell Reports MedicineAugust 18, 2026Cornelia D. Cudrici, Shubham Goel, Keiko Sakamoto, Seon-Pil Jin, Akiko Sekiguchi, Douglas R. Rosing, Rebecca Huffstutler, Dima A. Hammoud, Lee Shapiro, Daniella M. Schwartz, et al.Solution),Biolegend,Cat# 422302; RRID:AB_2818986 Zombie Violet™ Fixable Viability Kit,Biolegend,Cat# 423114 Rabbit anti-human CD4 (SP35),Roche (Ventana),Cat# 790-4423; RRID: AB_2335982 Rabbit anti-human CD8

(5) Localized immunomodulation with cytokine-producing cells to mitigate foreign body responses in rodents and a nonhuman primateSci AdvAugust 7, 2026Boram Kim, Dilrasbonu Vohidova, Amanda Nash, Yuen San Chan, Samantha Fleury, Shravani Deo, Danna Murungi, Peter D. Rios, Ira Joshi, Hafsa Nasir, et al.described in the fig. S11. For mouse samples, cells were stained with viability dye (1:1000; catalog no. 423114, BioLegend), anti-mouse CD11b (1:50; 101224, BioLegend), anti-mouse CD206 (1:40; catalog no. 141716,

(6) Progressive suppression of DNA repair genes with persistent p53 target activation in doxorubicin-treated cardiomyocytesG3: Genes, Genomes, GeneticsAugust 5, 2026Emma M Pfortmiller, José A Gutiérrez, Alyssa R Bogar, Michelle C WardEssex, CT, USA). Cells were stained with a viability marker, Zombie Violet Fixable Dye, diluted in PBS (423113, BioLegend, San Diego, CA, USA) for 30 min at 4 °C in the dark. Cells were washed with PBS and then

(7) Transcriptional regulation of disease-relevant microglial activation programsNeuronJuly 29, 2026Amanda McQuade, Reet Mishra, Venus Hagan, Weiwei Liang, Peter J. Colias, Vincent Cele Castillo, Bianca Gonzalez, Justin P. Lubin, Verena Haage, Victoria Marshe, et al.express,Gibco,Cat#12605-028 BODIPY,ThermoFisher,Cat#D3922 Zombie Violet Fixable Viability Kit,Biolegend,Cat #423113 Zombie Red Fixable Viability Kit,Biolegend,Cat#423110 Zombie Green Fixable Viability Kit,Biolegend,Cat#423112 "DMEM/F-12, HEPES, no phenol red

(8) Extracellular vesicles from cytokine-treated human pancreatic ductal cells enhance HLA class I expression in beta cellsFront ImmunolJuly 23, 2026Neslihan Erdem, Heather N. Zook, Janine C. Quijano, Nathaniel P. Hansen, Joanna Palade, Eunjin Oh, Jacob Mares, Cecile Donohue, Jose A. Ortiz, Kevin Jou, et al.discrimination, EndoC-βH1 cells were washed with PBS and incubated with Zombie Violet Dead Cell Stain (Biolegend, 423113) diluted 1:1, 000 in PBS for 15 min at room temperature. Cells were washed with PBS and resuspended

(9) Non-reproductive tumor progression impairs ovarian function through CXCL10-IL18R1 axisExp Mol MedJuly 22, 2026Shi-Ya Xie, Shu-Ping Zhang, Cong-Rong Li, Yan Liu, Xian-Zhe Gu, Yan-Jie Yang, Zhi-Xia Yang, Qian Wang, Hong-Jie Yao, Yun-Xia Cao, et al.live & dead viability via a Zombie Violet™ Fixable Viability Kit (BioLegend, Beijing, China; Cat. No. 423113) for 20 min, followed by incubation with an anti-CD16/CD32 mAb (BioLegend; Cat. No. 156603) to block

(10) Stemness-associated MEF-derived extruded nanovesicles cooperate with Lactobacillus rhamnosus to alleviate DSS-induced colitis through mucosal microenvironment remodelingNanoscale AdvJuly 21, 2026Xinyu Cao, Chao Yan, Qing Ji, Yutong Pan, Wencheng Shi, Huijun Zhao, Aihua Gong, Zhengzou Fangreagents were used for flow cytometry analysis: Zombie Violet™ Fixable Viability Kit (1:500, BioLegend, 423113, California, USA), PE-CD3 (0.5 µg/106 cells, Thermo Fisher Scientific, AB_2815096, MA, USA), APC-CD4

(11) MDI1228, a topical pan-JAK inhibitor, disrupts dermal fibroblast-T cell chemokine crosstalk to resolve allergic contact and atopic dermatitisFront ImmunolJuly 20, 2026Youxi Liu, Meimei Yin, Yichun Yang, Shujun Heng, Wenlu Zhang, Jinqiu Song, Binxing Feng, Yihan Yang, Wenjie Liu, Ling-Juan Zhanghr. before staining. Viability was assessed using Zombie Violet™ Fixable Viability Kit (BioLegend, #423114) to exclude dead cells. After Fc receptor blockade with anti-mouse CD16/32 antibody (eBioscience, #14016185),

(12) Limitations of EBV transformed human Raji B cells as a model for measuring canonical NF-κB activationbioRxivJuly 11, 2026Rachel Kidwell, Christopher D. ScharerKit (Lonza, V4XC-3012). Edited cells were surface stained with a fixable viability dye (BioLegend, 423113) prior to fixation/permeabilization (Thermofisher, 88-8824-00). Intracellular staining was then performed

(13) A screen of chromatin-targeting compounds identifies TAF1 as a novel regulator of HIV latencymBioJuly 8, 2026Samuel D. Burgos, Airlie M. Ward, Manickam Ashokkumar, Kimberly P. Enders, Lindsey I. James, David M. Margolis, Edward P. Browne553262), depending on the model system. Cell viability was determined using Zombie Violet dye (BioLegend, 423113), diluted 1:1,000 in phosphate-buffered saline (PBS) (Thermo Scientific, 14190144). Cells were washed

(14) Metabolomics of in vivo inflammation identifies soluble sialic acid as a conserved myeloid-cell metabolitebioRxivJuly 7, 2026Helen A. Jordan, Joseph A. Tandurella, Vineeth Vengayil, Tanay S. Parnaik, Swechha Mainali Mainali Pokharel, Kathleen Mulka, Sara Cherry, E. John Wherry, Caroline R. Bartmanaliquoted into 96 well plates and centrifuged at 1800 rpm for 2 mins at 4°C. Live Dead Violet (Biolegend, 423113) master mix was diluted 1:500 in phosphate buffered saline and added to all wells. A positive control

(15) A self-assembled protein nanocage as a universal influenza vaccine induces enhanced broadly cross-reactive immunityBiomaterialsJuly 1, 2026Jaeyoung Park, Sydney C. Wimberley, Thomas Pho, Mariela R. Rodriguez-Otero, Julie A. ChampionRestimulated splenocytes were then stained with Zombie Violet fixable viability dye (BioLegend, cat: 423113) as per manufacturer’s instructions. After washing, splenocytes were blocked with 5 μL/106 cells Trustain

(16) A Myocyte‐Enriched Long Non‐Coding RNA NRMLncR Enhances Myogenesis in MouseFASEB JJuly 1, 2026Yufen Li, Yumei Zhou, Qing Ying Li, Justine Arrington, Zhengzheng Hu, Mostafa F. Abdelhai, Yubo Wang, Junxiao Ren, Yung‐Yu Cheng, Mingyi Xie, et al.as previously described [48]. Cells were stained with a Zombie Violet Live/Dead dye (BioLegend, cat#423113) to exclude dead cells and subjected to FACS analysis. SCs were sorted based on GFP fluorescence and

(17) Engineering nanoparticle surface chemistry for antigen-presenting cell targeting improves specificity and safety of TLR3 agonist cancer immunotherapybioRxivJune 26, 2026Victoria F. Gomerdinger, Cassandra Parada, Andrea Li, Aidan Kindopp, Justin A. Kaskow, Eva Cai, Julia B. Treese, Ivan S. Pires, Apoorv Shanker, Gil Covarrubias, et al.media. Cells were stained with 1:600 dilution of Zombie Violet or Zombie NIR viability stain (Biolegend #423113, 423105) in 100 μl of PBS for 10 min at room temperature, subsequently fixed in 2% formaldehyde for

(18) NRF2-mediated inhibition of alveolar macrophage MHC II expression during Mycobacterium tuberculosis infectionCell ReportsJune 23, 2026Linh K. Pham, Maritza M. Cervantes, Pamelia N. Lim, Divya Dubey, Ankita Tufts, M. Clare Mitchell, Tomoyo Shinkawa, Samuel M. Behar, Alissa C. Rothchild(clone PO3),BioLegend,Cat# 105114; RRID: AB_313167 Zombie Violet™ Fixable Viability Kit,BioLegend,Cat# 423113 Anti-Nrf2(D1Z9) Rabbit mAntibody,Cell signaling,Cat #12721S Isotype control antibody Rabbit (DA1E)

(19) Protocol for co-culture of murine bone marrow-derived dendritic cells with OT-I mouse CD8+ T cells to evaluate immune activation efficacySTAR ProtocJune 19, 2026Min Jiang, Dana Matzek, Anny Nguyen, Bastian Popper, Olivia M. Merkel(Sigma-Aldrich),Cat. no. E4884 Zombie Violet™ Fixable Viability Kit (Dilution: 1 μL in 100 μL PBS),BioLegend,Cat. no. 423113 CellTrace™ CFSE Cell Proliferation Kit,BioLegend,Cat. no. 423801 "Monensin Solution (1,000×)",BioLegend,Cat.

(20) Tumor immune microenvironment reconstitution in patient-derived organoids enables therapy modeling for NSCLCCell Reports MethodsJune 15, 2026Enrique Podaza, Jared Capuano, Hui-Hsuan Kuo, Majd Al Assaad, Geoffrey Markowitz, M. Victoria Revuelta, John Nguyen, Adriana Irizarry, Hiranmayi Ravichandran, Sarah Ackermann, et al.proteins" ,, Human recombinant IL-2,Peprotech,200–02 Zombie Violet Fixable viability kit,Biolegend,423114 Zombie UV Fixable viability kit,Biolegend,423108 Cell trace Far red,Thermo Fisher Scientific,34564 NucView

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10/17/2019Zombie Violet Viability Dye Kabir KhanFollow the manufacturer's instructions to be able to stain live cells in flow cytometry for cells that need to be fixed and permeabilised to be able to stain intracellular proteins.

Zombie Violet™ Fixable Viability Kit from BioLegend

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Zombie Violet™ Fixable Viability Kit from BioLegend

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