Glycogen Assay Kit II (Colorimetric) ab169558 from Abcam

Product Specs
ItemGlycogen Assay Kit II (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab169558
Quantity100 Test
Detection Range4 - 40 µg/ml
Sample TypeTissue, Adherent cells, Suspension cells
SensitivityMore than 4 µg/mL
Assay Time1h 0m
Research AreaMetabolism

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Hepatic metabolic reprogramming in male mice during short-term caloric restriction involves enhanced glucocorticoid rhythmsNat CommunDecember 11, 2025Konstantinos Makris, Vlera Fonda, Fania Feby Ramadhani, Lina Fadel, Morgane Davezac, Bertrand Payet, Ioannis K. Deligiannis, Liwei Zhang, Teresa Horn, Laura Heimerl, et al.hydrolysis buffer. The hepatic supernatants were also measured using a colorimetric assay kit (Abcam, #ab169558), adhering to the manufacturer’s instructions. Ploidy measurement Paraffin-embedded liver samples

(2) Intergenerational effects of dietary iron on swimming and metabolic performance in zebrafishFront PhysiolOctober 16, 2025Theanuga Chandrapalan, Suhani Walia, Raymond W. M. Kwongquantified in the carcass using colorimetric kits from Abcam following the manufacturer’s instructions (Ab169558 and Ab65336). 2.4 Swimming and metabolic performance Two Loligo miniswim tunnel respirometers (Loligo®

(3) Intergenerational effects of dietary iron on swimming and metabolic performance in zebrafishFrontiers in PhysiologyOctober 16, 2025Theanuga Chandrapalan, Suhani Walia, Raymond W. M. Kwongquantified in the carcass using colorimetric kits from Abcam following the manufacturer’s instructions (Ab169558 and Ab65336). 2.4 Swimming and metabolic performance Two Loligo miniswim tunnel respirometers (Loligo®

(4) Amygdala-liver signalling orchestrates glycaemic responses to stressNatureOctober 1, 2025Carty JRE, Devarakonda K, O''Connor RM, Krek A, Espinoza D, Jimenez-Gonzalez M, Alvarsson A, Hampton RF, Li R, Qiu Y, et al.(Abnova KA1877) were determined by ELISA. Liver glycogen was determined by colorimetric assay (Abcam ab169558). Circulating glycerol and triglyceride levels were measured by enzymatic assay (Sigma-Aldrich TR0100).

(5) Ppp1r3b is a metabolic switch that shifts hepatic energy storage from lipid to glycogenSci AdvMay 16, 2025Kate Townsend Creasy, Minal B. Mehta, Carolin V. Schneider, Joseph Park, David Zhang, Swapnil V. Shewale, John S. Millar, Marijana Vujkovic, Nicholas J. Hand, Paul M. Titchenell, et al.Hepatic glycogen levels were measured by Glycogen Assay Kit II (Colorimetric) from Abcam (catalog no. ab169558). Tissues were homogenized with distilled H2O on ice and then boiled for 10 min. Homogenates were spun

(6) Hepatic Deletion of Carbohydrate Response Element Binding Protein Impairs Hepatocarcinogenesis in a High-Fat Diet-Induced Mouse ModelInt J Mol SciMarch 3, 2025Majedul Karim, Jessica Prey, Franziska Willer, Helen Leiner, Mohd Yasser, Frank Dombrowski, Silvia Ribbackincluding glycogen, triglyceride, and total cholesterol were measured using commercial assay kits (Cat. No. ab169558 for glycogen, Abcam, Cambridge, UK; Cat. No. E-BC-K261-M for Triglyceride; and Cat. No. E-BC-K109-M

(7) TNFα-CXCR1/2 partners in crime in insulin resistance conditionsCell Death DiscoveryDecember 3, 2024Vanessa Castelli, Housem Kacem, Laura Brandolini, Cristina Giorgio, Marta Sofia Scenna, Marcello Allegretti, Annamaria Cimini, Michele d’Angelotreated as described above and glycogen assay was performed following manufacturer’s instruction (Abcam, #ab169558). For the sample preparation cells were rapidly homogenized on ice and then boiled for 10 mins to inactivate

(8) Short‐chain fatty acids enhance muscle mass and function through the activation of mTOR signalling pathways in sarcopenic miceJournal of Cachexia, Sarcopenia and MuscleDecember 1, 2024Chaoran Liu, Pui Yan Wong, Qianjin Wang, Hei Yuet Wong, Tao Huang, Can Cui, Ning Zhang, Wing Hoi Cheung, Ronald Man Yeung Wongquantification Glycogen contents of QUA muscle and liver tissue in mice were quantified with Glycogen Assay Kit II (ab169558, Abcam, Cambridge, UK) following the manufacturer's instructions. Colon, muscle, and heart section

(9) PKM2 regulates metabolic flux and oxidative stress in the murine heartPhysiological ReportsSeptember 10, 2024Katie C. Y. Lee, Allison L. Williams, Lu Wang, Guoxiang Xie, Wei Jia, Anastasia Fujimoto, Mariana Gerschenson, Ralph V. Shohetusing the Glucose‐Glo Assay kit (Promega, cat.# J6021) and the Glycogen Assay Kit II (Abcam, cat.# ab169558), respectively. ATP was measured using the ATP Determination Kit (Invitrogen, cat.# A22066). Equivalent

(10) Glycogen myophosphorylase loss causes increased dependence on glucose in iPSC-derived retinal pigment epitheliumThe Journal of biological chemistryAugust 1, 2024Basu B, Karwatka M, China B, McKibbin M, Khan K, Inglehearn CF, Ladbury JE, Johnson CA.reader. Glycogen content measurement Glycogen measurements of cells were done using a kit (AbCam, ab169558) according to the manufacturer’s instructions. RPE cells grown on inserts and allowed to mature were

(11) Impaired Fat Absorption from Intestinal Tract in High-Fat Diet Fed Male Mice Deficient in Proglucagon-Derived PeptidesNutrientsJuly 14, 2024Koki Nishida, Shinji Ueno, Yusuke Seino, Shihomi Hidaka, Naoya Murao, Yuki Asano, Haruki Fujisawa, Megumi Shibata, Takeshi Takayanagi, Kento Ohbayashi, et al.the supernatant was analyzed using a Glycogen Colorimetric Assay Kit II (Abcam, Tokyo, Japan, Cat# ab169558). 2.7. Immunoblotting Analysis Immunoblotting analysis was carried out as previously reported [30]

(12) Mis-localization of endogenous TDP-43 leads to ALS-like early-stage metabolic dysfunction and progressive motor deficitsMolecular neurodegenerationJune 20, 2024Hu Y, Hruscha A, Pan C, Schifferer M, Schmidt MK, Nuscher B, Giera M, Kostidis S, Burhan Ö, van Bebber F, et al.homogenizer (KONTES®). Glycogen was quantified with the Glycogen Assay Kit II (colorimetric) (Abcam, ab169558) according to the manufacturer’s instructions. Statistics Statistical analyses were performed using

(13) The Metabolic and Endocrine Effects of a 12-Week Allulose-Rich DietNutrientsJune 10, 2024Kevin B. Cayabyab, Marley J. Shin, Micah S. Heimuli, Iris J. Kim, Dominic P. D’Agostino, Richard J. Johnson, Andrew P. Koutnik, Nick Bellissimo, David M. Diamond, Nicholas G. Norwitz, et al.according to the manufacturer’s protocol. Glycogen levels were assessed using a liver glycogen assay kit (ab169558; Abcam). Triglyceride and glycogen content was normalized to liver wet weight. Plasma levels of alanine

(14) Amygdala-liver signaling orchestrates rapid glycemic responses to stress and drives stress-induced metabolic dysfunctionResearch SquareMarch 30, 2024Sarah Stanley, Kavya Devarakonda, Richard O'Connor, Maria Jimenez-Gonzalez, Alexandra Alvarsson, Rollie Hampton, Diego Espinoza, Rosemary Li, Abigail Shtekler, Kaetlyn Conner, et al.(Abnova #KA1877) were determined by ELISA. Liver glycogen was determined by colorimetric assay (Abcam #ab169558). Circulating glycerol and triglyceride levels were measured by enzymatic assay (Sigma-Aldrich # TR0100). Western blot

(15) How Does CBG Administration Affect Sphingolipid Deposition in the Liver of Insulin-Resistant Rats?NutrientsOctober 12, 2023Wiktor Bzdęga, Piotr Franciszek Kurzyna, Ewa Harasim-Symbor, Adam Hołownia, Adrian Chabowski, Karolina Konstantynowicz-Nowickatissue homogenates was evaluated using a commercially available kit (Glycogen Assay Kit II; cat no.: ab169558, Abcam, Cambridge, UK). The above analysis was based on the colorimetric method and was performed according

(16) Mutant IDH regulates glycogen metabolism from early cartilage development to malignant chondrosarcoma formationCell reportsJune 27, 2023Pathmanapan S, Poon R, De Renshaw TB, Nadesan P, Nakagawa M, Seesankar GA, Ho Loe AK, Zhang HH, Guinovart JJ, Duran J, et al.20 transmission electron microscope. Glycogen quantification Abcam Glycogen Assay Kit II (Abcam, ab169558) was used to quantify glycogen levels in pulverized PDX chondrosarcomas and patient chondrosarcoma cells.

(17) Can Maternal Exposure to Air Pollution Affect Post-Natal Liver Development?ToxicsJanuary 9, 2023Yong Song, Ling Chen, Ellen Bennett, Amanda J. Wheeler, Katherine Southam, Seiha Yen, Fay Johnston, Graeme R. ZoskyMetabolism Biochemical quantification of hepatic glycogens in the liver was performed using a glycogen assay kit II (ab169558, Abcam, Melbourne, Australia). Liver tissue (10 mg) was homogenised with 200 µL of ddH2O for 10 min

(18) Altered electrical properties in skeletal muscle of mice with glycogen storage disease type IIScientific ReportsDecember 1, 2022Janice A. Nagy, Carson Semple, Daniela Riveros, Benjamin Sanchez, Seward B. Rutkovethe muscle (~ 10–15 mg) was later analyzed for GC, using the Glycogen Assay Kit II Colorimetric Kit (#Ab169558, Abcam, Cambridge, MA) and a microplate reader (Fisherbrand accuSkan GO UV/Vis Microplate Spectrophotometer,

(19) N6-methyladenosine modification governs liver glycogenesis by stabilizing the glycogen synthase 2 mRNANature communicationsNovember 17, 2022Zhang X, Yin H, Zhang X, Jiang X, Liu Y, Zhang H, Peng Y, Li D, Yu Y, Zhang J, et al.materials and the supernatant was ready for the assay using a Glycogen Assay Kit II (colorimetric) (Abcam, ab169558), and the results were normalized to the weight of initial tissues. Serum glucose detection (mice/rats) Normal

(20) Dynamic glucose uptake, storage, and release by human microvascular endothelial cellsMolecular biology of the cellOctober 1, 2022Yazdani S, Bilan PJ, Jaldin-Fincati JR, Pang J, Ceban F, Saran E, Brumell JH, Freeman SA, Klip A.quantification HAMEC and Huh-7 cellular glycogen content was determined using a colorimetric assay kit (ab169558, Abcam). Typically, 106 cells were pelleted, boiled in 200 µl of water for 10 min, homogenized, and

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Glycogen Assay Kit II (Colorimetric) from Abcam

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Glycogen Assay Kit II (Colorimetric) from Abcam

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