NAD/NADH Assay Kit (Fluorometric) ab176723 from Abcam

Product Specs
ItemNAD/NADH Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab176723
Quantity250 test
Sample TypeCell culture extracts, Tissue Extracts
Assay Time2h 30m
ApplicationsCellular Assay
ReactivityMammals, Mammals, Other species
TypeQuantitative
Detection TargetNAD/NADH

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Adaptive responses of neuronal cells to chronic endoplasmic reticulum (ER) stressRedox BiologyNovember 1, 2023Thu Nguyen Minh Pham, Natarajan Perumal, Caroline Manicam, Marion Basoglu, Stefan Eimer, Dominik C. Fuhrmann, Claus U. Pietrzik, Albrecht M. Clement, Hagen Körschgen, Jana Schepers, et al.adenine dinucleotide (NAD+/NADH) assay The ratio of NAD+/NADH was determined by fluorometric assay kit (ab176723, Abcam). Briefly, 25 μl of NAD/NADH control buffer or NADH extraction buffer was added to lysate samples,

(2) Live Cell Imaging of ATP Levels Reveals Metabolic Compartmentalization within Motoneurons and Early Metabolic Changes in FUS ALS MotoneuronsCellsMay 9, 2023Vitaly L. Zimyanin, Anna-Maria Pielka, Hannes Glaß, Julia Japtok, Dajana Großmann, Melanie Martin, Andreas Deussen, Barbara Szewczyk, Chris Deppmann, Eli Zunder, et al.Measurements The experiment was performed according to the manufacturer’s description (NAD+/NADH Assay Kit, Abcam, ab176723). A total of 5–7 × 106 cells were scraped into PBS and centrifuged for 5 min at room temperature (1500

(3) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.NADH concentrations NADH absolute concentrations were measured using a fluorometric assay (Abcam, ab176723) according to the manufacturer’s protocol. At least 500,000 cells were collected per condition. Floating

(4) Modulation of sirtuins during monolayer chondrocyte culture influences cartilage regeneration upon transfer to a 3D culture environmentFrontiers in Bioengineering and BiotechnologyDecember 6, 2022Hannah K. Heywood, Stephen D. Thorpe, Renos M. Jeropoulos, Paul W. Caton, David A. LeeNMN to increase chondrocyte NAD levels was confirmed using a commercial fluorometric NAD assay kit (ab176723, Abcam, Cambridge, United Kingdom) after 4 h exposure using the manufacturer’s methods. Chondrocytes

(5) Tachycardiomyopathy entails a dysfunctional pattern of interrelated mitochondrial functionsBasic Research in CardiologyDecember 1, 2022Michael G. Paulus et al.state Mitochondrial redox state NADH/NAD+ in rabbit LV was determined by a fluorometric assay kit (ab176723, Abcam). Tissue redox state in LV was assessed as the ratio of glutathione (GSH) to glutathione disulfide

(6) Effects of Calcium Ions on the Antimicrobial Activity of Gramicidin ABiomoleculesDecember 1, 2022Shang-Ting Fang, Shu-Hsiang Huang, Chin-Hao Yang, Jen-Wen Liou, Hemalatha Mani, Yi-Cheng Chenfluorescein was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). NAD/NADH Assay kit (ab176723) was purchased from Abcam PLC (Cambridge, UK). All other chemicals were reagent grade and used without

(7) Swim Training Affects on Muscle Lactate Metabolism, Nicotinamide Adenine Dinucleotides Concentration, and the Activity of NADH Shuttle Enzymes in a Mouse Model of Amyotrophic Lateral SclerosisInternational Journal of Molecular SciencesSeptember 29, 2022Karol Cieminski, Damian Jozef Flis, Katarzyna Patrycja Dzik, Jan Jacek Kaczor, Mariusz Roman Wieckowski, Jedrzej Antosiewicz, Wieslaw ZiolkowskiTibialis Anterior Muscle All procedures were conducted according to an NAD+/NADH Assay Kit (Abcam, ab176723) using chemicals supplied by the manufacturer. Briefly, tissues (20 mg of tibialis anterior muscle)

(8) Expression of the mono-ADP-ribosyltransferase ART1 by tumor cells mediates immune resistance in non-small cell lung cancerScience translational medicineMarch 16, 2022Wennerberg E, Mukherjee S, Spada S, Hung C, Agrusa CJ, Chen C, Valeta-Magara A, Rudqvist NP, Van Nest SJ, Kamel MK, et al.supernatants were then tested for inhibition of purified human ART1 by a fluorescent NAD+ readout (Abcam, cat. ab176723). Anti-ART1 hybridoma supernatants were also tested for inhibition of human ART1 transiently expressed

(9) Hepatocyte membrane potential regulates serum insulin and insulin sensitivity by altering hepatic GABA releaseCell reportsJune 29, 2021Geisler CE, Ghimire S, Hepler C, Miller KE, Bruggink SM, Kentch KP, Higgins MR, Banek CT, Yoshino J, Klein S, et al.T7531, Pointe Scientific Inc., Canton, MI). Hepatic NADH and NAD were quantified by fluorometric assay (ab176723, Abcam, Cambridge, UK). Hepatic ATP concentrations were assessed as previously described (Geisler et

(10) Targeting the gut microbial metabolic pathway with small molecules decreases uremic toxin productionGut MicrobesNovember 9, 2020Yingyi Wang et al.centrifugation at 10,000 rpm for 5 min, and then NADH/NAD+ was measured using an NAD/NADH assay kit (Abcam, Cat# ab176723). E. coli was incubated with 200 μM ISO for 0.25– 2 h. Intracellular ATP was measured in the collected

(11) Aging alters acetylation status in skeletal and cardiac musclesGeroScienceJune 1, 2020Yeo D, Kang C, Ji LL.activity assay Total NAD+/NADH and NAD+ levels were measured according to manufacturer’s instruction (ab176723, NAD+/NADH Assay kit, Abcam). Briefly, 25 μl of NAD/NADH control buffer or NADH extraction buffer was

(12) Characterization of the Role of the Malate Dehydrogenases to Lung Tumor Cell SurvivalJournal of CancerJuly 5, 2017Boxi Zhang, Johan Tornmalm, Jerker Widengren, Helin Vakifahmetoglu-Norberg, Erik Norbergassay The experiment was performed according to manufacturer's description (NAD+/NADH Assay Kit, Abcam, ab176723). 7 x106 cells were scraped in ice cold PBS and centrifuged for 5 min at room temperature. 5x106 cells

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NAD/NADH Assay Kit (Fluorometric) from Abcam

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NAD/NADH Assay Kit (Fluorometric) from Abcam

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