NAD/NADH Assay Kit (Fluorometric) ab176723 from Abcam

Product Specs
ItemNAD/NADH Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab176723
Quantity250 Test
Sample TypeCell culture extracts, Tissue Extracts
Assay Time2h 30m

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Redox index and capacity analysis (RICA) reveals NAD biology changes in T cell responses in vitro and ex vivoJ ImmunolApril 15, 2026Alicia M. Bostwick-Galaviz, Edward J. UsherwoodIsolated mitochondria were lysed in NAD/NADH Lysis Buffer from the Abcam NAD/NADH Fluorometric Assay Kit (ab176723) at 37 °C for 15 minutes, then centrifuged at 2,000 × g for 5 minutes at 4 °C. NADH and total NAD+/NADH

(2) NAD + -Boosting Ameliorates Heme Protein-Mediated Acute Kidney InjuryKidney360March 1, 2026Raman Deep Singh, Anthony J. Croatt, Joseph P. Grande, Allan W. Ackerman, Trace A. Christensen, Luis A. Juncos, Karl A. Nathan enzyme cycling reaction, performed for these studies with a fluorometric assay kit (catalog no. ab176723; Abcam) according to the manufacturer's instructions.19 Briefly, frozen kidney tissues were homogenized

(3) Manganese (III) tetrakis (4-benzoic acid) porphyrin (MnTBAP) represses sulfide:quinone oxidoreductase expression and targets the sulfido-redox system in glioblastoma modelsRedox ReportDecember 31, 2025Elise Malard, Benoît Bernay, Jérôme Toutain, Samantha Ballesta, Marie Lévêque, Julien Pontin, Samuel Valable, Myriam Bernaudin, Laurent Chatrethe L-Lactate Assay kit (MAK443, Sigma-Aldrich), NAD+/NADH content and ratio with NAD/NADH Assay Kit (AB176723, ABCAM), GSH/GSSG content and ratio with the GSH/GSSG Assay Kit (MAK440, Sigma-Aldrich), and total ATP

(4) Lactate as a metabolic–epigenetic signal linking high-intensity interval training (HIIT) to miRNA-Centered remodeling of the skeletal muscle methylome and transcriptomeRedox BiologyDecember 1, 2025Lei Zhou, Soroosh Mozaffaritabar, Kumpei Tanisawa, Takuji Kawamura, Mitsuru Higuchi, Istvan Boldogh, Xueqing Ba, Sataro Goto, George Brooks, Yaodong Gu, et al.measured using assay kits (Abcam; lactate kit ab65330, pyruvate kit ab65342, αKG kit ab83431, NAD/NADH kit ab176723). Approximately 20 ± 2 mg of homogenized tissue from the pre-mixed gastrocnemius of six mice per group

(5) NAD+-Boosting Ameliorates Heme Protein–Mediated Acute Kidney InjuryKidney360November 25, 2025Raman Deep Singh, Anthony J. Croatt, Joseph P. Grande, Allan W. Ackerman, Trace A. Christensen, Luis A. Juncos, Karl A. Nathan enzyme cycling reaction, performed for these studies with a fluorometric assay kit (catalog no. ab176723; Abcam) according to the manufacturer's instructions.19 Briefly, frozen kidney tissues were homogenized

(6) Endothelial soluble epoxide hydrolase links polyunsaturated fatty acid metabolism to oxidative stress and atherosclerosis progressionRedox BiologySeptember 1, 2025Minghua Cao, Mengqing Li, Xixuan Li, Yuanyuan Li, Yunyun Chen, Maria-Kyriaki Drekolia, Xiang Cheng, Fredy Delgado Lagos, Sofia-Iris Bibli, Jiong Huhuman umbilical vein endothelial cells with a commercially available NAD/NADH Assay kit from Abcam (Berlin, Germany, Cat.No. ab176723) according to the manufacturers protocol as previously described [22]. The values of NADH and NAD+ were

(7) The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LRCell Death DisJuly 3, 2025Xiaoya Sun, Qinmei Tan, Yue Yang, Jia Wei, Xiaodie Zhou, Shanshan Gao, Fei YangKit(Sangon Biotech, D799099), Glucose Uptake Assay Kit(Abcam, ab136956), NAD+/NADH Assay Kit(Abcam, ab176723), MitoTox Complex I OXPHOS Activity Microplate Assay (AbCam, ab109903), and ROS Assay Kit (Nanjing jiancheng

(8) MIA40 suppresses cell death induced by apoptosis-inducing factor 1EMBO ReportsMarch 7, 2025Ben Hur Marins Mussulini, Klaudia K Maruszczak, Piotr Draczkowski, Mayra A Borrero-Landazabal, Selvaraj Ayyamperumal, Artur Wnorowski, Michal Wasilewski, Agnieszka Chacinska7.1). NADH/NAD+ balance Total NADH/NAD+ levels were measured accordingly to manufacturer instruction (AB176723, NAD+/NADH Assay Kit, Abcam) and as previously described in the literature (Yeo et al, 2020). Detection

(9) Mitochondrial translocase TOMM22 is overexpressed in pancreatic cancer and promotes aggressive growth by modulating mitochondrial protein import and functionMol Cancer ResFebruary 1, 2024Mary Oluwadamilola Haastrup, Kunwar Somesh Vikramdeo, Shashi Anand, Mohammad Aslam Khan, James Elliot Carter, Seema Singh, Ajay Pratap Singh, Santanu Dasguptaabove. NAD+/NADH measurement NAD+/NADH ratio was determined using the NAD+/NADH quantification kit (Abcam #ab176723) according to the protocol provided by the manufacturer. Cells were lysed in lysis buffer, after which,

(10) Mitochondrial Translocase TOMM22 Is Overexpressed in Pancreatic Cancer and Promotes Aggressive Growth by Modulating Mitochondrial Protein Import and FunctionMolecular Cancer ResearchFebruary 1, 2024Mary Oluwadamilola Haastrup, Kunwar Somesh Vikramdeo, Shashi Anand, Mohammad Aslam Khan, James Elliot Carter, Seema Singh, Ajay Pratap Singh, Santanu Dasguptaabove. NAD+/NADH measurement NAD+/NADH ratio was determined using the NAD+/NADH quantification kit (Abcam #ab176723) according to the protocol provided by the manufacturer. Cells were lysed in lysis buffer, after which,

(11) Loss of cardiac mitochondrial complex I persulfidation impairs NAD+ homeostasis in agingRedox BiologyFebruary 1, 2024Maria-Kyriaki Drekolia, Christina Karantanou, Ilka Wittig, Yuanyuan Li, Dominik C. Fuhrmann, Bernhard Brüne, Antonia Katsouda, Jiong Hu, Andreas Papapetropoulos, Sofia-Iris BibliHEK293 cells with a commercially available NAD/NADH Assay kit from Abcam (Berlin, Germany, Cat.No. ab176723) according to the manufacturers protocol. The values of NADH and NAD+ were normalized to the amount

(12) Adaptive responses of neuronal cells to chronic endoplasmic reticulum (ER) stressRedox biologyNovember 1, 2023Pham TNM, Perumal N, Manicam C, Basoglu M, Eimer S, Fuhrmann DC, Pietrzik CU, Clement AM, Körschgen H, Schepers J, et al.adenine dinucleotide (NAD+/NADH) assay The ratio of NAD+/NADH was determined by fluorometric assay kit (ab176723, Abcam). Briefly, 25 μl of NAD/NADH control buffer or NADH extraction buffer was added to lysate samples,

(13) Live Cell Imaging of ATP Levels Reveals Metabolic Compartmentalization within Motoneurons and Early Metabolic Changes in FUS ALS MotoneuronsCellsMay 9, 2023Vitaly L. Zimyanin, Anna-Maria Pielka, Hannes Glaß, Julia Japtok, Dajana Großmann, Melanie Martin, Andreas Deussen, Barbara Szewczyk, Chris Deppmann, Eli Zunder, et al.Measurements The experiment was performed according to the manufacturer’s description (NAD+/NADH Assay Kit, Abcam, ab176723). A total of 5–7 × 106 cells were scraped into PBS and centrifuged for 5 min at room temperature (1500

(14) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.NADH concentrations NADH absolute concentrations were measured using a fluorometric assay (Abcam, ab176723) according to the manufacturer’s protocol. At least 500,000 cells were collected per condition. Floating

(15) Modulation of sirtuins during monolayer chondrocyte culture influences cartilage regeneration upon transfer to a 3D culture environmentFrontiers in Bioengineering and BiotechnologyDecember 6, 2022Hannah K. Heywood, Stephen D. Thorpe, Renos M. Jeropoulos, Paul W. Caton, David A. LeeNMN to increase chondrocyte NAD levels was confirmed using a commercial fluorometric NAD assay kit (ab176723, Abcam, Cambridge, United Kingdom) after 4 h exposure using the manufacturer’s methods. Chondrocytes

(16) Tachycardiomyopathy entails a dysfunctional pattern of interrelated mitochondrial functionsBasic Research in CardiologyDecember 1, 2022Michael G. Paulus et al.state Mitochondrial redox state NADH/NAD+ in rabbit LV was determined by a fluorometric assay kit (ab176723, Abcam). Tissue redox state in LV was assessed as the ratio of glutathione (GSH) to glutathione disulfide

(17) Effects of Calcium Ions on the Antimicrobial Activity of Gramicidin ABiomoleculesDecember 1, 2022Shang-Ting Fang, Shu-Hsiang Huang, Chin-Hao Yang, Jen-Wen Liou, Hemalatha Mani, Yi-Cheng Chenfluorescein was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). NAD/NADH Assay kit (ab176723) was purchased from Abcam PLC (Cambridge, UK). All other chemicals were reagent grade and used without

(18) Swim Training Affects on Muscle Lactate Metabolism, Nicotinamide Adenine Dinucleotides Concentration, and the Activity of NADH Shuttle Enzymes in a Mouse Model of Amyotrophic Lateral SclerosisInternational Journal of Molecular SciencesSeptember 29, 2022Karol Cieminski, Damian Jozef Flis, Katarzyna Patrycja Dzik, Jan Jacek Kaczor, Mariusz Roman Wieckowski, Jedrzej Antosiewicz, Wieslaw ZiolkowskiTibialis Anterior Muscle All procedures were conducted according to an NAD+/NADH Assay Kit (Abcam, ab176723) using chemicals supplied by the manufacturer. Briefly, tissues (20 mg of tibialis anterior muscle)

(19) Expression of the mono-ADP-ribosyltransferase ART1 by tumor cells mediates immune resistance in non-small cell lung cancerScience translational medicineMarch 16, 2022Wennerberg E, Mukherjee S, Spada S, Hung C, Agrusa CJ, Chen C, Valeta-Magara A, Rudqvist NP, Van Nest SJ, Kamel MK, et al.supernatants were then tested for inhibition of purified human ART1 by a fluorescent NAD+ readout (Abcam, cat. ab176723). Anti-ART1 hybridoma supernatants were also tested for inhibition of human ART1 transiently expressed

(20) Hepatocyte membrane potential regulates serum insulin and insulin sensitivity by altering hepatic GABA releaseCell reportsJune 29, 2021Geisler CE, Ghimire S, Hepler C, Miller KE, Bruggink SM, Kentch KP, Higgins MR, Banek CT, Yoshino J, Klein S, et al.T7531, Pointe Scientific Inc., Canton, MI). Hepatic NADH and NAD were quantified by fluorometric assay (ab176723, Abcam, Cambridge, UK). Hepatic ATP concentrations were assessed as previously described (Geisler et

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NAD/NADH Assay Kit (Fluorometric) from Abcam

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NAD/NADH Assay Kit (Fluorometric) from Abcam

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