Fig 1: RT-PCR analyses of MMP2, MMP9 and hTERT mRNA expression in A-549 cells after treatment with 24 for 48 h. Expression was normalised to RNA polymerase subunit (RPII). Error bars represent the SEM of data obtained in n = 3 independent experiments. *p < 0.01, **p < 0.001, ***p < 0.0001, ****p < 0.00001 vs. vehicle (two-way ANOVA and post hoc Dunnett’s test).
Fig 2: Inhibitory activity of selected S-substituted imidazole-2-thione derivatives against MMP-9. Bars represent IC50 values calculated from three independent experiments (mean ± SD).
Fig 3: Binding pose of compound 24 on MMP2 and 9 (a), position of compound 24 on 10 snapshots from 500 ns MDrun (b). RMSD of ligand and protein (c) MMP2 (d) MMP9. A timeline representation of the interactions of (e) MMP2 (f) MMP9 shows which residues interact with the ligand in each trajectory frame. Some residues make more than one specific contact with the ligand, which is represented by a darker shade of orange. The contributions of various energy components to the total binding energy of the drug-Protein complexes (g) MMP2 (h) MMP9. Representation of the interactions and contacts (H-bonds, Hydrophobic, Ionic, Water bridges) of (i) MMP2 (j) MMP9.
Supplier Page from Abcam for MMP9 Inhibitor Screening Assay Kit (Fluorometric)