NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2) E7580S from New England Biolabs

Product Specs
ItemNEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2)
CompanyNew England Biolabs
Price $1,515.00 Inquire
Catalog NumberE7580S
Quantity24 reactions
ApplicationsDNA Analysis, Multiplex, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Transcriptomic Response to Neuromuscular Electrical Stimulation in Muscle, Brain, and Plasma EVs in WT and Klotho-Deficient MiceInt J Mol SciAugust 14, 2025Catherine Anne Cavanaugh, Amanda E. Moore, Nicholas Francis Fitz, Iliya Lefterov, Radosveta Koldamovalibraries were produced by New England BioLabs’ NEBNext Multiplex Small RNA Library Prep Set (E7300S and E7580S, Ipswich, MA, USA). Library quality was assessed on samples by the High Sensitivity DNA Assay Kit for

(2) Small RNA in sperm-Paternal contributions to human embryo developmentNat CommunJuly 17, 2025Signe Isacson, Kajsa Karlsson, Stefan Zalavary, Anna Asratian, Unn Kugelberg, Susanne Liffner, Anita Östlibraries were produced with NEBNext Multiplex SmallRNA Library Prep Kit for Illumina (E7300S, E7330S, E7580S; New England Biolabs), with the customisation of a dilution of primers 1:8. The 3´adaptor ligation reaction

(3) Key Amniotic Fluid miRNAs as Promising Target Molecules for the Antenatal Prevention of Pulmonary Hypoplasia Associated with Congenital Diaphragmatic HerniaInt J Mol SciApril 19, 2025Angelika V. Timofeeva, Ivan S. Fedorov, Yuri I. Naberezhnev, Nana K. Tetruashvili, Gennady T. SukhikhRNA Library Prep Set for Illumina® (Set1, New England Biolabs®, Frankfurt am Main, Germany, cat. No. E7580S), following the manufacturer’s protocol. The cDNA libraries were amplified for 21 cycles and purified

(4) Impaired hippocampal neurogenesis associated with regulatory ceRNA network in a mouse model of postoperative cognitive dysfunctionBMC AnesthesiolFebruary 6, 2025Jingrun Lin, Xiaoqiu Zhu, Xuan Li, Yu Hong, Yaohui Liang, Siqi Chen, Chenzhuo Feng, Lin Caopassing quality checks. For miRNA, the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2) (E7580S, NEB, United States) was utilized to ligate the 3’ and 5’ ends of the extracted RNA. Following reverse

(5) Universal First-Trimester Screening Biomarkers for Diagnosis of Preeclampsia and Placenta Accreta SpectrumBiomoleculesFebruary 4, 2025Timofeeva AV, Fedorov IS, Tarasova AM, Sukhova YV, Kolod'ko VG, Ivanets TY, Sukhikh GT.RNA Library Prep Set for Illumina® (Sets 1 and 2, New England Biolabs®, Frankfurt, Germany, cat. nos. E7300S and E7580S). Libraries were amplified for 19 cycles, purified using the QIAQuick PCR Purification Kit (Qiagen,

(6) Increased Levels of hsa-miR-199a-3p and hsa-miR-382-5p in Maternal and Neonatal Blood Plasma in the Case of Placenta Accreta SpectrumInternational Journal of Molecular SciencesDecember 11, 2024Angelika V. Timofeeva, Ivan S. Fedorov, Anastasia D. Nikonets, Alla M. Tarasova, Ekaterina N. Balashova, Dmitry N. Degtyarev, Gennady T. SukhikhRNA Library Prep Set for Illumina® (Set2, New England Biolab®, Frankfurt am Main, Germany, cat. No. E7580S), following the manufacturer’s protocol. The cDNA libraries were amplified and purified using 6% polyacrylamide

(7) Cockayne Syndrome Linked to Elevated R-Loops Induced by Stalled RNA Polymerase II during Transcription ElongationNature CommunicationsJuly 17, 2024Xuan Zhang, Jun Xu, Jing Hu, Sitao Zhang, Yajing Hao, Dongyang Zhang, Hao Qian, Dong Wang, Xiang-Dong Fuand then constructed into a library using the NEBNext® Multiplex Small RNA Library Prep kit (NEB Cat# E7580S) Cell fractionation and RNA-seq library preparation For the chromatin-associated RNA-seq, chromatin-associated

(8) Diagnostic Role of Cell-Free miRNAs in Identifying Placenta Accreta Spectrum during First-Trimester ScreeningInternational Journal of Molecular SciencesJanuary 10, 2024Angelika V. Timofeeva, Ivan S. Fedorov, Yuliya V. Suhova, Alla M. Tarasova, Larisa S. Ezhova, Tatyana M. Zabelina, Oksana N. Vasilchenko, Tatyana Y. Ivanets, Gennady T. Sukhikhfor Illumina® (Set11 and Set2, New England Biolab®, Frankfurt am Main, Germany, cat. nos. E7300S and E7580S), amplified for 19 cycles in case of blood plasma samples, 21 PCR cycles in case of blood serum exosomes,

(9) IsoSeek for unbiased and UMI-informed sequencing of miRNAs from low input samples at single-nucleotide resolutionSTAR ProtocolsDecember 1, 2023Monique A.J. van Eijndhoven, Chantal Scheepbouwer, Ernesto Aparicio-Puerta, Michael Hackenberg, D. Michiel Pegtel,Thermo Fisher Scientific,Cat#4366597 NEBNext Multiplex Small RNA Library Prep Set for Illumina,New England Biolabs,Cat#E7300/E7580/E7560 Monarch PCR & DNA Cleanup Kit,New England Biolabs,Cat#T1030 S/L KAPA Library Quantification

(10) Starvation induces changes in abundance and small RNA cargo of extracellular vesicles released from Plasmodium falciparum infected red blood cellsScientific reportsOctober 27, 2023Vetter L, Bajalan A, Ahamed MT, Scasso C, Shafeeq S, Andersson B, Ribacke U.libraries were prepared by using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB #E7580) according to the instructions of the manufacturer. Libraries were quantified using the Collibri™ Library

(11) RA-induced prominence-specific response resulted in distinctive regulation of Wnt and osteogenesisLife Sci AllianceAugust 4, 2023Chao Song, Ting Li, Chunlei Zhang, Shufang Li, Songhui Lu, Yi Zousequencing libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (Cat.No. E7580S; New England Biolabs) and were sequenced at an average read depth of 18–20 million reads per sample

(12) Blood Plasma Small Non-Coding RNAs as Diagnostic Molecules for the Progesterone-Receptor-Negative Phenotype of Serous Ovarian TumorsInternational Journal of Molecular SciencesJuly 30, 2023Angelika V. Timofeeva, Ivan S. Fedorov, Aleksandra V. Asaturova, Maya V. Sannikova, Anna V. Tregubova, Oleg A. Mayboroda, Grigory N. Khabas, Vladimir E. Frankevich, Gennady T. SukhikhSet for Illumina® (Set11 and Set2, New England Biolab®, Frankfurt am Main, Germany, cat. No. E7300S, E7580S). After amplification for 14 PCR cycles and purification in the 6% polyacrylamide gel, cDNA libraries

(13) Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of lightiScienceJuly 21, 2023Celiker C, Weissova K, Cerna KA, Oppelt J, Dorgau B, Gambin FM, Sebestikova J, Lako M, Sernagor E, Liskova P, et al.4304437 NEBNext Multiplex Small RNA Library Prep Set for Illumina,New England Biolabs,Cat# E7300S; Cat# E7580S Human Pluripotent Stem Cell Transcription Factor Kit,BD Biosciences,Cat #560589 NextSeq 500/550 High

(14) MicroRNA Profiling of Self-Renewing Human Neural Stem Cells Reveals Novel Sets of Differentially Expressed microRNAs During Neural Differentiation In VitroStem Cell Reviews and ReportsJuly 1, 2023Veronika Fedorova, Katerina Amruz Cerna, Jan Oppelt, Veronika Pospisilova, Tomas Barta, Marek Mraz, Dasa Bohaciakovaused for library preparation. NEBNext Multiplex Small RNA Library Prep Set for Illumina (#E7300S, #E7580S Set1, 2; New England Biolabs) was used to prepare libraries for further sequencing according to the

(15) Dietary Sugar Shifts Mitochondrial Metabolism and Small RNA Biogenesis in SpermAntioxidants & Redox SignalingJune 1, 2023Rashmi Ramesh, Signe Skog, Lovisa Örkenby, Unn Kugelberg, Daniel Nätt, Anita ÖstRNA libraries were produced with NEBNext Multiplex SmallRNA Library Prep Kit for Illumina (E7560S, E7580; New England Biolabs), with the customization of a dilution of primers 1:2. The 3´adaptor ligation reaction

(16) Prediction of Early- and Late-Onset Pre-Eclampsia in the Preclinical Stage via Placenta-Specific Extracellular miRNA ProfilingInternational Journal of Molecular SciencesApril 28, 2023Angelika V. Timofeeva, Ivan S. Fedorov, Yuliya V. Sukhova, Tatyana Y. Ivanets, Gennady T. Sukhikhfor Illumina® (Set11 and Set2, New England Biolab®, Frankfurt am Main, Germany, cat. nos. E7300S and E7580S), amplified for 19 and 14 PCR cycles, respectively, and sequenced using the NextSeq 500 platform (Illumina,

(17) Search for New Participants in the Pathogenesis of High-Grade Serous Ovarian Cancer with the Potential to Be Used as Diagnostic MoleculesLife (Basel)December 3, 2022Angelika V. Timofeeva, Aleksandra V. Asaturova, Maya V. Sannikova, Grigory N. Khabas, Vitaliy V. Chagovets, Ivan S. Fedorov, Vladimir E. Frankevich, Gennady T. SukhikhSet for Illumina® (Set11 and Set2, New England Biolab®, Frankfurt am Main, Germany, cat. No. E7300S, E7580S), amplified for 14 and 18 PCR cycles, respectively, and sequenced on the NextSeq 500 platform (Illumina,

(18) Upregulation of Spinal miR-155-5p Contributes to Mechanical Hyperalgesia by Promoting Inflammatory Activation of Microglia in Bone Cancer Pain RatsLife (Basel)August 30, 2022Yanping Jian, Zongbin Song, Zhuofeng Ding, Jian Wang, Ruike Wang, Xinran HouRNA purification, library construction using NEBNext® Multiplex Small RNA Library Prep Set for Illumina (Cat# E7580S, NEB, Ipswich, MA, USA), and miRNA sequencing that was performed on Illumina NovaSeq 6000 system conducted

(19) Microglia Regulate Blood-Brain Barrier Integrity via MiR-126a-5p/MMP9 Axis during Inflammatory DemyelinationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)August 1, 2022Yu Z, Fang X, Liu W, Sun R, Zhou J, Pu Y, Zhao M, Sun D, Xiang Z, Liu P, et al.using a NEB Next Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA, E7580S) following the manufacturer's instructions. Individual libraries were analyzed for the presence of linked

(20) A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNANature communicationsMay 30, 2022Van Bortle K, Marciano DP, Liu Q, Chou T, Lipchik AM, Gollapudi S, Geller BS, Monte E, Kamakaka RT, Snyder MP.libraries were generated using the NEBnext small RNA library preparation kit according to instructions (E7580S, New England BioLabs). Immunoblotting THP-1 lysates were extracted using the mirVana PARIS RNA and Native Protein Purification kit

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NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2)

NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2) from New England Biolabs

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NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2) from New England Biolabs

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