NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2) E7580L from New England Biolabs

Product Specs
ItemNEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2)
CompanyNew England Biolabs
Price $5,149.00 Inquire
Catalog NumberE7580L
Quantity96 reactions
ApplicationsDNA Analysis, Multiplex, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) IsoSeek for unbiased and UMI-informed sequencing of miRNAs from low input samples at single-nucleotide resolutionSTAR ProtocolsDecember 1, 2023Monique A.J. van Eijndhoven, Chantal Scheepbouwer, Ernesto Aparicio-Puerta, Michael Hackenberg, D. Michiel Pegtel,Thermo Fisher Scientific,Cat#4366597 NEBNext Multiplex Small RNA Library Prep Set for Illumina,New England Biolabs,Cat#E7300/E7580/E7560 Monarch PCR & DNA Cleanup Kit,New England Biolabs,Cat#T1030 S/L KAPA Library Quantification

(2) Starvation induces changes in abundance and small RNA cargo of extracellular vesicles released from Plasmodium falciparum infected red blood cellsScientific reportsOctober 27, 2023Vetter L, Bajalan A, Ahamed MT, Scasso C, Shafeeq S, Andersson B, Ribacke U.libraries were prepared by using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB #E7580) according to the instructions of the manufacturer. Libraries were quantified using the Collibri™ Library

(3) RA-induced prominence-specific response resulted in distinctive regulation of Wnt and osteogenesisLife Sci AllianceAugust 4, 2023Chao Song, Ting Li, Chunlei Zhang, Shufang Li, Songhui Lu, Yi Zousequencing libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (Cat.No. E7580S; New England Biolabs) and were sequenced at an average read depth of 18–20 million reads per sample

(4) Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of lightiScienceJuly 21, 2023Celiker C, Weissova K, Cerna KA, Oppelt J, Dorgau B, Gambin FM, Sebestikova J, Lako M, Sernagor E, Liskova P, et al.4304437 NEBNext Multiplex Small RNA Library Prep Set for Illumina,New England Biolabs,Cat# E7300S; Cat# E7580S Human Pluripotent Stem Cell Transcription Factor Kit,BD Biosciences,Cat #560589 NextSeq 500/550 High

(5) MicroRNA Profiling of Self-Renewing Human Neural Stem Cells Reveals Novel Sets of Differentially Expressed microRNAs During Neural Differentiation In VitroStem Cell Reviews and ReportsJuly 1, 2023Veronika Fedorova, Katerina Amruz Cerna, Jan Oppelt, Veronika Pospisilova, Tomas Barta, Marek Mraz, Dasa Bohaciakovaused for library preparation. NEBNext Multiplex Small RNA Library Prep Set for Illumina (#E7300S, #E7580S Set1, 2; New England Biolabs) was used to prepare libraries for further sequencing according to the

(6) Dietary Sugar Shifts Mitochondrial Metabolism and Small RNA Biogenesis in SpermAntioxidants & Redox SignalingJune 1, 2023Rashmi Ramesh, Signe Skog, Lovisa Örkenby, Unn Kugelberg, Daniel Nätt, Anita ÖstRNA libraries were produced with NEBNext Multiplex SmallRNA Library Prep Kit for Illumina (E7560S, E7580; New England Biolabs), with the customization of a dilution of primers 1:2. The 3´adaptor ligation reaction

(7) Upregulation of Spinal miR-155-5p Contributes to Mechanical Hyperalgesia by Promoting Inflammatory Activation of Microglia in Bone Cancer Pain RatsLife (Basel)August 30, 2022Yanping Jian, Zongbin Song, Zhuofeng Ding, Jian Wang, Ruike Wang, Xinran HouRNA purification, library construction using NEBNext® Multiplex Small RNA Library Prep Set for Illumina (Cat# E7580S, NEB, Ipswich, MA, USA), and miRNA sequencing that was performed on Illumina NovaSeq 6000 system conducted

(8) Microglia Regulate Blood-Brain Barrier Integrity via MiR-126a-5p/MMP9 Axis during Inflammatory DemyelinationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)August 1, 2022Yu Z, Fang X, Liu W, Sun R, Zhou J, Pu Y, Zhao M, Sun D, Xiang Z, Liu P, et al.using a NEB Next Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA, E7580S) following the manufacturer's instructions. Individual libraries were analyzed for the presence of linked

(9) A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNANature communicationsMay 30, 2022Van Bortle K, Marciano DP, Liu Q, Chou T, Lipchik AM, Gollapudi S, Geller BS, Monte E, Kamakaka RT, Snyder MP.libraries were generated using the NEBnext small RNA library preparation kit according to instructions (E7580S, New England BioLabs). Immunoblotting THP-1 lysates were extracted using the mirVana PARIS RNA and Native Protein Purification kit

(10) Analysis of miRNAs responsive to longâ€term calcium deficiency in tef ( Eragrostis tef (Zucc.) Trotter)Plant DirectMay 10, 2022Muhammad Numan, Wanli Guo, Sangâ€Chul Choi, Xuegeng Wang, Boxuan Du, Weibo Jin, Ramji Kumar Bhandari, Ayalew Ligabaâ€OsenaMultiplex small RNA library preparation set according to user instructions for the Illumina (E7300 and E7580, NEB). Sequencing was performed using Illumina Hiseq2500 platform using 50‐nt read length with single

(11) Obesity and Roux-en-Y gastric bypass drive changes in miR-31 and miR-215 expression in the human rectal mucosaInternational Journal of ObesityFebruary 1, 2022Stella Panagio Breininger, Laura Sabater, Fiona Caroline Malcomson, Sorena Afshar, Jelena Mann, John Cummings MathersRNA Library Prep Set for Illumina® (Set 1 (catalog number/ ID: E7300S) and Set 2 (catalog number/ ID: E7580S), New England BioLabs)) and sequenced using the Illumina MiSeq sequencer (MiSeq Reagent Kit v3). Raw

(12) Dual roles for piRNAs in promoting and preventing gene silencing in C. elegansCell ReportsDecember 7, 2021Brooke E. Montgomery, Tarah Vijayasarathy, Taylor N. Marks, Charlotte A. Cialek, Kailee J. Reed, Taiowa A. Montgomery(Human/Mouse/Rat),Illumina,Cat# MRZH11124 NEBNext Small RNA Library Prep Kit for Illumina,New England Biolabs,"Cat# E7300S, E7580S" TURBO DNA-free Kit,Thermo Fisher Scientific,Cat# AM1907 SuperScript III Reverse Transcriptase,Thermo

(13) MiRNA post-transcriptional modification dynamics in T cell activationiScienceJune 25, 2021Rodríguez-Galán A, Dosil SG, Gómez MJ, Fernández-Delgado I, Fernández-Messina L, Sánchez-Cabo F, Sánchez-Madrid F.miRNeasy Mini Kit,Qiagen,Cat#217004 NEBNext Multiplex SmallRNA Library Prep Set for Illumina,New England Biolabs,Cat#E7580L miRCURY LNA RT Kit,Qiagen,Cat#339340 miRCURY LNA SYBER Green PCR Kit,Qiagen,Cat#339347 "miRCURY LNA

(14) Identification of the Potential Gene Regulatory Networks and Therapeutics in Aged Mice With Postoperative Neurocognitive DisorderFrontiers in NeuroscienceJune 24, 2021Wensi Wu, Yongpai Peng, Jiaxin Zhou, Xiaojun Zhang, Lin Cao, Wei-jye Lin, Yanan Lu, Jing Wen, Zhi Wangtotal RNAs were performed by the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2) (E7580S, NEB, United States). After the amplification, the quality, and concentration of library were measured

(15) A novel diagnostic signature based on three circulating exosomal mircoRNAs for chronic obstructive pulmonary diseaseExperimental and Therapeutic MedicineMay 3, 2021Yahui Shen, Lina Wang, Yunhui Wu, Yingwei Ou, Huiyu Lu, Xin Yaosmall RNA libraries using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina (cat. no. E7580; New England BioLabs® Inc.). Libraries were sequenced using HiSeq 2500 (Illumina, Inc.) with single‑end

(16) Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damageTheranosticsJanuary 1, 2021Luo Q, Xian P, Wang T, Wu S, Sun T, Wang W, Wang B, Yang H, Yang Y, Wang H, et al.generated using 1 µg of total RNA based on the NEBNext Multiplex Small RNA Library Prep Set from Illumina (E7580S, NEB, USA) per the manufacturer's instructions. The quality of the cDNA library was assessed using a

(17) Halofuginone inhibits tumorigenic progression of 5‑FU‑resistant human colorectal cancer HCT‑15/FU cells by targeting miR‑132‑3p in vitroOncology LettersOctober 23, 2020Chen Wang, Jian-Bin Zhu, Yan-Yan Yan, Wei Zhang, Xiao-Jie Gong, Xia Wang, Xiao-Liang Wangusing the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs Inc.; NEB #E7580L), which contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed

(18) MicroRNA-200a induces immunosuppression by promoting PTEN-mediated PD-L1 upregulation in osteosarcomaAgingJanuary 24, 2020Liu Z, Wen J, Wu C, Hu C, Wang J, Bao Q, Wang H, Wang J, Zhou Q, Wei L, et al.kit (217004, Qiagen). miRNA-Seq libraries were performed using the Small RNA Library Prep (NEB E7300L/E7580L). miRNA-seq libraries were run on the HiSeq 2500 (Illumina) using Single-end 50-bp sequencing. miR-Seq

(19) henn-1/HEN1 Promotes Germline Immortality in Caenorhabditis elegansCell ReportsDecember 3, 2019Joshua M. Svendsen, Kailee J. Reed, Tarah Vijayasarathy, Brooke E. Montgomery, Rachel M. Tucci, Kristen C. Brown, Taylor N. Marks, Dieu An H. Nguyen, Carolyn M. Phillips, Taiowa A. Montgomery(Human/Mouse/Rat),Illumina,MRZH11124 NEBNext Small RNA Library Prep Kit for Illumina,NEB,E7300S or E7580S Deposited Data,Deposited Data,Deposited Data NGS data,This Study,Gene Expression Omnibus GSE137734 Experimental Models

(20) Obesity impacts the regulation of miR-10b and its targets in primary breast tumorsBMC CancerDecember 1, 2019Ari Meerson, Yaniv Eliraz, Hila Yehuda, Bridget Knight, Malcolm Crundwell, Douglas Ferguson, Benjamin P. Lee, Lorna W. Harriesusing the NEB Next Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs Cat. NEB-E7580S). The libraries were sequenced with one lane on an Illumina NextSeq 500 instrument with 50-bp single-end

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NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2)

NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2) from New England Biolabs

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NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2) from New England Biolabs

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