Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Assay Kits
Assay Kit
Zombie NIR(TM) Fixable Viability Kit
Zombie NIR(TM) Fixable Viability Kit from BioLegend
(4)
Write a review
Citations:
(513)
Supplier Page
Supplier Page from
BioLegend for
Zombie NIR(TM) Fixable Viability Kit
Description
Zombie NIR(TM) Fixable Viability Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Zombie NIR(TM) Fixable Viability Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
423106
Quantity
500 tests
Applications
Flow Cytometry (FC)
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (513)
(1)
Role of affinity in plasma cell development in the germinal center light zone
Journal of Experimental Medicine
January 1, 2024
Mohamed A. ElTanbouly, Victor Ramos, Andrew J. MacLean, Spencer T. Chen, Maximilian Loewe, Sandra Steinbach, Tarek Ben Tanfous, Brianna Johnson, Melissa Cipolla, Anna Gazumyan,
et al.
1A8-
Ly6g
,
47-9668-82
). Dead cells were identified and excluded using live/dead Zombie
NIR
(
BioLegend
,
423106
). For sorting DZ and LZ GC B cells sorting (Fig. 4), anti-Igλ1 (BD Biosciences,
clone
R26-46,
744526
),
(2)
In vitro Assessment of Efferocytic Capacity of Human Macrophages Using Flow Cytometry
Bio Protoc
December 20, 2023
Ana C.G. Salina, Marlon Fortes-Rocha, Larissa D. Cunha
(
Sigma-Aldrich
, catalog number:
H4522
-100ml)Zombie
NIR
Fixable Viability (
BioLegend
, catalog number:
423106
)ACK lysis buffer (see Recipes) Recipes ACK lysis Buffer 0.15 M NH4Cl10 mM KHCO30.1 mM EDTASolvent: deionized
(3)
Hepatitis D infection induces IFN-β-mediated NK cell activation and TRAIL-dependent cytotoxicity
Front Immunol
December 8, 2023
Christopher Groth, Jovana Maric, Irene Garcés Lázaro, Tomáš Hofman, Zhenfeng Zhang, Yi Ni, Franziska Keller, Isabelle Seufert, Maike Hofmann, Christoph Neumann-Haefelin,
et al.
130-059-901
). Live cells were identified using the Zombie NIR™ Fixable Viability Kit (
Biolegend
, Cat.:
423105
) Zombie Aqua™ Fixable Viability Kit (
Biolegend
, Cat.:
423101
), fixable viability dye e780 (eBioscience,
(4)
Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes
STAR Protocols
December 1, 2023
Yudai Xu, Zijian Wang, Wen Gao, Haoyun Wang, Zhixian Wang, Chenxuan Tian, Guobing Chen
7-AAD Viability Staining Solution
,
BioLegend
,Cat#
420404
Zombie NIR Fixable Viability Kit,
BioLegend
,Cat#
423105
Brain heart infusion (BHI),Oxoid,Cat# CM1135 Agar,Biosharp,Cat# BS195-1k
Cell Activation Cocktail (with Brefeldin A
(5)
Protocol for isolation of signet ring cells from human gastric mucosa
STAR Protocols
December 1, 2023
Sarah G. Samaranayake, Lauren A. Gamble, Cassidy Bowden, Benjamin L. Green, Amber F. Gallanis, Dilara Akbulut, Niharika Shah, Jonathan M. Hernandez, Jeremy L. Davis
gastric mucosa
demonstrates a viable sample. 80.1% viability with Zombie Live-Dead
staining
(
BioLegend
Cat#
423105
) with significant cytokeratin expression via Alexa Fluor 647-conjugated pan-cytokeratin monoclonal antibody
(6)
RIPK3 promotes brain region-specific interferon signaling and restriction of tick-borne flavivirus infection
PLOS Pathogens
November 27, 2023
Marissa Lindman, Juan P. Angel, Irving Estevez, Nydia P. Chang, Tsui-Wen Chou, Micheal McCourt, Colm Atkins, Brian P. Daniels
clone
1A8), Ly6C (Biolegend,
clone
HK1.4), CD80 (Biolegend,
clone
16-10A1), and Zombie
NIR
(
Biolegend
, #
423105
).
Leukocytes
were stained for 30 minutes at 4C prior to washing in FACS buffer and fixation with 1%
(7)
Epidermal keratinocyte-specific STAT3 deficiency aggravated atopic dermatitis-like skin inflammation in mice through TSLP upregulation
Frontiers in Immunology
November 20, 2023
Zhao-Yuan Wang, Yu-Xin Zheng, Fan Xu, Ying-Zhe Cui, Xue-Yan Chen, Si-Qi Chen, Bing-Xi Yan, Yuan Zhou, Min Zheng, Xiao-Yong Man
(
1:100
, BioLegend, San Diego, CA), the cells were incubated with Live/Dead Zombie dye (UV,
BioLegend
,
423106
,
1:100
) and antibodies specific for
CD45
(BV510,
BioLegend
,
103138
,
1:100
) and FcϵRIα (PE/Cyanine7,
(8)
More time to kill: a longer liver stage increases T cell-mediated protection against pre-erythrocytic malaria
iScience
November 20, 2023
Naveen Yadav, Chaitra Parthiban, Zachary P. Billman, Brad C. Stone, Felicia N. Watson, Kevin Zhou, Tayla M. Olsen, Irene Cruz Cruz Talavera, Annette Mariko Seilie, Anya C. Kalata,
et al.
Cells were blocked with Fc CD16/32 (
clone
2.4G2, BD) containing
1:1000
Zombie viability dye (cat #
423105
,
BioLegend
) for 30 minutes. Surface
staining
was done by incubating cells for 20 minutes on ice with
(9)
Ly6C-high monocytes alleviate brain injury in experimental subarachnoid hemorrhage in mice
Journal of Neuroinflammation
November 17, 2023
Huaijun Chen, Chaoran Xu, Hanhai Zeng, Zhihua Zhang, Ning Wang, Yinghan Guo, Yonghe Zheng, Siqi Xia, Hang Zhou, Xiaobo Yu,
et al.
antibody and assessing the live or dead status of the cells using with Zombie NIR™ Fixable Viability Kit (
423106
,
BioLegend
, USA), the cells were incubated with cell surface antibodies (Table 2) for 30 min at 4 ℃.
(10)
Radiotherapy combined with docetaxel alters the immune phenotype of HNSCC cells and results in increased surface expression of CD137 and release of HMGB1 of specifically HPV-positive tumor cells
Neoplasia
November 1, 2023
Fridolin Grottker, Simon Gehre, Clara M. Reichardt, Azzaya Sengedorj, Tina Jost, Thorsten Rieckmann, Markus Hecht, Antoniu-Oreste Gostian, Benjamin Frey, Rainer Fietkau,
et al.
Diego, CA, USA" TNFRSF9/CD 137,BV 421,
311508
,
BioLegend
,"San Diego, CA, USA" Live/dead,Zombie
NIR
,
423105
,
BioLegend
,"San Diego, CA, USA" List of
flow cytometry
antibodies and dyes for immune checkpoint molecule
(11)
PD-L1-expressing cancer-associated fibroblasts induce tumor immunosuppression and contribute to poor clinical outcome in esophageal cancer
Cancer immunology, immunotherapy : CII
November 1, 2023
Kawasaki K, Noma K, Kato T, Ohara T, Tanabe S, Takeda Y, Matsumoto H, Nishimura S, Kunitomo T, Akai M,
et al
.
were also used. Dead cells (
1:1000
dilution) were stained using a Zombie NIR Fixable Viability Kit (
423106
,
BioLegend
). Stained cells were analyzed using
flow cytometry
(FACSLyric; BD Biosciences, Franklin Lakes,
(12)
PKR-mediated stress response enhances dengue and Zika virus replication
mBio
October 31, 2023
Taissa Ricciardi-Jorge, Edroaldo Lummertz da Rocha, Edgar Gonzalez-Kozlova, Gabriela Flavia Rodrigues-Luiz, Brian J. Ferguson, Trevor Sweeney, Nerea Irigoyen, Daniel Santos Mansur
GADD34 antibody
(Proteintech;
10449-1-AP
, dilution
1:250
[IF/FACS]); Zombie
NIR
fixable viability dye (
BioLegend
;
423105
, dilution
1:750
); goat anti-rabbit IgG (H+L) Alexa Fluor 647 conjugated (
Invitrogen
;
A-21245
, dilution
(13)
Tumor cell-induced platelet aggregation accelerates hematogenous metastasis of malignant melanoma by triggering macrophage recruitment
Journal of experimental & clinical cancer research : CR
October 23, 2023
Chen Y, Zhou J, Liu Z, Wu T, Li S, Zhang Y, Yin X, Yang G, Zhang G.
(
BioLegend
, catalog no.
426102
, USA)], and Zombie NIR Fixable Viability Kit (
BioLegend
, catalog no.
423105
, USA). The RedFluor™ 710 Anti-Mouse CD45 (30-F-11) (TONBO Biosciences, catalog no. 80–0451, USA),
PerCP-Cyanine5.5 anti-Mouse F4/80 antigen (BM8.1
(14)
CXCL13 promotes thermogenesis in mice via recruitment of M2 macrophage and inhibition of inflammation in brown adipose tissue
Frontiers in Immunology
October 23, 2023
Lijun Xie, Huiying Wang, Dan Wu, Feng Zhang, Wei Chen, Yuqing Ye, Fang Hu
Flow cytometry
Single cells of SVF fraction were resuspended with PBS and stained with zombie
NIR
™ dye (#
423105
,
Biolegend
, USA), blocked with FcX Blocking CD16/32 (#
156604
), and dyed with a mix of surface marker
(15)
The admixed brushtail possum genome reveals invasion history in New Zealand and novel imprinted genes
Nature Communications
October 17, 2023
Donna M. Bond, Oscar Ortega-Recalde, Melanie K. Laird, Takashi Hayakawa, Kyle S. Richardson, Finlay.C. B. Reese, Bruce Kyle, Brooke E. McIsaac-Williams, Bruce C. Robertson, Yolanda van Heezik,
et al.
for 2 min). The resulting pellet was resuspended in Zombie Near-Infra Red Viability Dye (
BioLegend
,
423105
) and incubated at room temperature for 20 min. The lysate was washed in 5% FBS (in PBS), then resuspended
(16)
Targeting myeloid-derived suppressor cells with gemcitabine to enhance efficacy of adoptive cell therapy in bladder cancer
Frontiers in Immunology
October 12, 2023
Sarah Bazargan, Brittany Bunch, Awino Maureiq E. Ojwang‘, Jamie Blauvelt, Annick Landin, Johannes Ali, Dominique Abrahams, Cheryl Cox, Amy M. Hall, Matthew S. Beatty,
et al.
Flow cytometry
staining
Human urine cells,
tumor
digest, and PBMCs were stained with Zombie
NIR
(
Biolegend
,
423106
) (
1:1000
dilution) for live/dead
staining
. Cells were incubated in the dark at room temperature for
(17)
SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
October 1, 2023
She X, Wu Q, Rao Z, Song D, Huang C, Feng S, Liu A, Liu L, Wan K, Li X,
et al
.
min. After washing and
centrifugation
, the samples were stained with Zombie UV Fixable Viability Kit (
423106
,
BioLegend
) to exclude dead cells. Then, the samples were stained with anti‐CD45‐PerCP antibody (
103129
,
(18)
The COPD GWAS gene ADGRG6 instructs function and injury response in human iPSC-derived type II alveolar epithelial cells
The American Journal of Human Genetics
October 1, 2023
Rhiannon B. Werder, Kayleigh A. Berthiaume, Carly Merritt, Marissa Gallagher, Carlos Villacorta-Martin, Feiya Wang, Pushpinder Bawa, Vidhi Malik, Shawn M. Lyons, Maria C. Basil,
et al.
Live cells were sorted on a MoFlo Astrios EQ (Beckman Coulter) by Zombie
NIR
exclusion (
Biolegend
, #
423106
) and hashed cells were pooled
1:1
prior to capture. Libraries were prepared as per the 10× Genomics
(19)
Intrinsic tumor resistance to CAR T cells is a dynamic transcriptional state that is exploitable with low-dose radiation
Blood Advances
September 26, 2023
Alexander B. Kim, Ssu-Yu Chou, Solomon Kang, Eric Kwon, Matthew Inkman, Jeff Szymanski, Neal Andruska, Cian Colgan, Jin Zhang, Joanna C. Yang,
et al.
used to block Fc receptors.
In vitro
viability after RT was assessed using Zombie
NIR
(
Biolegend
cat#
423106
) and Annexin V PerCP-Cy5.5 (
Biolegend
cat#
640936
) according to the manufacturer’s protocols. Data were
(20)
Sézary syndrome originates from heavily mutated hematopoietic progenitors
Blood Advances
September 26, 2023
Carly M. Harro, Kimberly B. Sprenger, Ricardo A. Chaurio, John J. Powers, Patrick Innamarato, Carmen M. Anadon, Yumeng Zhang, Subir Biswas, Gunjan Mandal, Jessica A. Mine,
et al.
(CD7-6B7, Cat#343116). Cell viability was assessed through
staining
with the Zombie
NIR
(
Biolegend
, Cat#
423106
) for 15 minutes in phosphate-buffered saline (PBS). Data were analyzed using FlowJo v10 software. Cell
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Write a Review
(1)
11/29/2023
Reliable Zombie NIR™ Fixable Viability Kit From BioLegend
Varaang Bhandula
The Zombie NIR viability kit works well for identifying dead cells from cell suspensions isolated from mouse lungs, spleen, and lymph nodes. I recommend storing at -20C to ensure the dye works well ...
Read Review
(1)
4/24/2021
Relatively Good Fixable Live/Dead Dye
Hong Sun
We are interested in characterizing the immune populations in secondary lymphoid organs and tumors under different knockout condition.
(1)
1/11/2021
Using Zombie NIR to Detect Dead Cells
Alejandro Prados
We are using the Zombie NIR to characterize the immune cells populations in the mouse intestine during chronic inflammation. This reagent is perfect to clearly separate dead and live cells.
(1)
3/1/2016
Cell Viability Kit for FC
Maria Carmela Speranza
This is a good and easy to use cell viability staining kit for flow cytometry.
<<
<
>
>>
Zombie NIR(TM) Fixable Viability Kit from BioLegend
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top