Fig 1: Downstream applications of isolated gastric mucosa(A) Dissociated gastric mucosa in HistoGel medium prepared for periodic acid-Schiff and E-cadherin double stain immunohistochemistry for a patient with CDH1 mutation and a wild-type negative control at 400× total magnification. Parietal cells are marked in blue, chief cells in green, and signet ring cells in red based on staining and morphology. Cells expressing E-cadherin are stained brown. Early signet ring cell lesions are E-cadherin negative and PAS positive.(B) Flow cytometry scatterplot of a single cell suspension of CDH1 gastric mucosa demonstrates a viable sample. 80.1% viability with Zombie Live-Dead staining (BioLegend Cat#423105) with significant cytokeratin expression via Alexa Fluor 647-conjugated pan-cytokeratin monoclonal antibody (BioLegend Cat #628604), as seen in gastric epithelium and signet ring cells.