CellTiter-Fluor Cell Viability Assay G6082 from Promega

Product Specs
ItemCellTiter-Fluor Cell Viability Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG6082
Detection TargetCell Viability
Quantity2 x 50ml
MethodAssay

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Lysine deserts and cullin-RING ligase receptors: Navigating untrodden paths in proteostasisiScienceNovember 17, 2023Natalia A. Szulc, Małgorzata Piechota, Lilla Biriczová, Pankaj Thapa, Wojciech Pokrzywaviability during the CHX assays, they were multiplexed with the CellTiter-Fluor Cell Viability Assay (G6080, Promega) following the manufacturer’s guidelines. Briefly, after 3-hour incubation with CHX, 20 μL

(2) Targeting human mitochondrial NAD(P)+-dependent malic enzyme (ME2) impairs energy metabolism and redox state and exhibits antileukemic activity in acute myeloid leukemiaCell Oncol (Dordr)October 1, 2023Kun-Chi Chen, I-Hsin Hsiao, Yu-Nan Huang, Yu-Tung Chou, Yi-Chun Lin, Ju-Yi Hsieh, Yung-Lung Chang, Kang-Hsi Wu, Guang-Yaw Liu, Hui-Chih HungNa2EA for 48 h. Using the CellTiter-Fluor™ Cell Viability Assay, cell viability was determined (#Cat. G6080, Promega, Madison, WI, USA). Using a BioTek® multi-mode microplate reader with Ex/Em = 490/505 nm, the

(3) Dual role of the adhesion G-protein coupled receptor ADRGE5/CD97 in glioblastoma invasion and proliferationJournal of Biological ChemistrySeptember 1, 2023Tatiana I. Slepak, Manuela Guyot, Winston Walters, Daniel G. Eichberg, Michael E. Ivandetermine the numbers of viable cells to each well, 20 μl of 5× CellTiter-Fluor solution (Promega, #G6080) was added. The plate was briefly mixed and incubated for another 30 min at 37 °C in CO2 incubator followed

(4) Suppression of the human malic enzyme 2 modifies energy metabolism and inhibits cellular respirationCommunications BiologyMay 22, 2023Ju-Yi Hsieh, Kun-Chi Chen, Chun-Hsiung Wang, Guang-Yaw Liu, Jie-An Ye, Yu-Tung Chou, Yi-Chun Lin, Cheng-Jhe Lyu, Rui-Ying Chang, Yi-Liang Liu, et al.number of viable cells in a population was determined using the CellTiter-Fluor™ Cell Viability Assay (G6080, Promega, Madison, WI, USA). On 96 well plates, 1 × 104 cells were seeded and incubated for 24 h in

(5) The Oligostilbene Gnetin H Is a Novel Glycolysis Inhibitor That Regulates Thioredoxin Interacting Protein Expression and Synergizes with OXPHOS Inhibitor in Cancer CellsInternational Journal of Molecular SciencesApril 23, 2023Shivendra Singh, Flavia De Carlo, Mohamed A. Ibrahim, Patrice Penfornis, Alan J. Mouton, Siddharth K. Tripathi, Ameeta K. Agarwal, Linda Eastham, David S. Pasco, Premalatha Balachandran, et al.Technologies, Santa Clara, CA, USA). CellTiter-Fluor™ Cell Viability Assay (Promega, Madison, WI, USA, Cat.# G6080) was performed following manufacturer protocol to measure live-cell protease activity in cells treated

(6) Combined inhibition of ACLY and CDK4/6 reduces cancer cell growth and invasionOncology ReportsDecember 22, 2022Brian Christopher Velez, Christopher Paul Petrella, Kathleen Helen DiSalvo, Keyi Cheng, Rebecca Kravtsov, Dorina Krasniqi, Nancy Ann KrucherPromega Corporation; all assays performed with n=6 or n=8): CellTiter-Fluor Cell Viability Assay (cat. no. G6080), utilized on T47D, MCF7, MDA-MBA-231, Panc1 and MiaPaCa2 cells; RealTime-Glo MT Cell Viability assay

(7) Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespanNature CommunicationsDecember 1, 2022Giovanna Grigolon et al.screening, additionally using the non-lytic CellTiter-Fluor Cell Viability Assay (Promega Corporation, #G6082) according to the manufacturer’s instructions and prior to measuring luciferase activity. Luciferase

(8) TSG101 associates with PARP1 and is essential for PARylation and DNA damageâ€induced NFâ€ÎºB activationThe EMBO JournalNovember 2, 2022Ahmet BuÄŸra Tufan, Katina Lazarow, Marina Kolesnichenko, Anje Sporbert, Jens Peter von Kries, Claus Scheidereitcells. CellTiter‐Fluor™ cell viability assay Cell viability was assayed using the CellTiter‐Fluor™ kit (Promega, G6081) according to the manufacturer's instructions. Briefly, cells were seeded onto 96‐well plates with white

(9) STUB1 is an intracellular checkpoint for interferon gamma sensingScientific reportsAugust 18, 2022Ng S, Lim S, Sim ACN, Mangadu R, Lau A, Zhang C, Martinez SB, Chandramohan A, Lim UM, Ho SSW, et al.for 6 days without media change, the CellTiter-Glo 2.0 (Promega, G9243) or CellTiter-Fluor (Promega, G6082) assay were performed according to manufacturer’ instructions. The luminescence or fluorescence (Ex:

(10) JAK inhibitor blocks COVID-19 cytokine-induced JAK/STAT/APOL1 signaling in glomerular cells and podocytopathy in human kidney organoidsJCI insightJune 8, 2022Nystrom SE, Li G, Datta S, Soldano KL, Silas D, Weins A, Hall G, Thomas DB, Olabisi OA.qRT-PCR; and the 24-well plate was fixed in 4% PFA for IHC. CellTiter-Fluor Cell Viability Assay (Promega, G6080) and CellTiter-Glo 2.0 Assay (Promega, G9241) were performed using standard protocol instructions provided

(11) In Vitro and In Vivo Analysis of Extracellular Vesicle-Mediated Metastasis Using a Bright, Red-Shifted Bioluminescent Reporter ProteinAdv Genet (Hoboken)March 1, 2022Perez GI, Broadbent D, Zarea AA, Dolgikh B, Bernard MP, Withrow A, McGill A, Toomajian V, Thampy LK, Harkema J, et al.determined 48 h after EV treatments by using the CellTiter‐Fluor cell viability assay kit from Promega (G6080) following manufacturer's instructions. The filters in the fluorescence plate reader were set for AFC

(12) Inhibition of Heme Export and/or Heme Synthesis Potentiates Metformin Anti-Proliferative Effect on Cancer Cell LinesCancers (Basel)February 27, 2022Anna Lucia Allocco, Francesca Bertino, Sara Petrillo, Deborah Chiabrando, Chiara Riganti, Alberto Bardelli, Fiorella Altruda, Veronica Fiorito, Emanuela Tolosanoanalyzed with the Cell Titer-Fluor™ Cell Viability Assay (Promega, Madison, WI, USA, catalogue No. G6080) according to manufacture instructions. 2.7. Crystal Violet Staining Experiments were performed as

(13) Loss of Function of WFS1 Causes ER Stress-Mediated Inflammation in Pancreatic Beta-CellsFrontiers in endocrinologyJanuary 1, 2022Morikawa S, Blacher L, Onwumere C, Urano F.without the indicated reagents. After the treatment, CellTiter-Fluor Cell Viability Assay (Promega, Cat# G6080) reagent was added directly to cells and incubated for 30 min in the dark. After the fluorescence measurement,

(14) Biological and therapeutic implications of a unique subtype of NPM1 mutated AMLNature CommunicationsDecember 1, 2021Arvind Singh Mer et al.each of the selected patient samples, and cell viability was measured with the Cell-Titer Fluor Assay (G6082, Promega) after 72 h incubation. The drug dose response data was processed in the R PharmacoGx package

(15) Tau phosphorylation and OPA1 proteolysis are unrelated events: Implications for Alzheimer's DiseaseBiochimica et biophysica acta. Molecular cell researchNovember 1, 2021Alavi MV.measured with the CellTiter-Glo assay (#G7570, Promega, Madison, WI, USA) and the CellTiter-Fluor assay (#G6080, Promega), respectively. Briefly, 2,000 Neuro2A cells per well of a 96-well half-area plate were incubated

(16) Salubrinal Exposes Anticancer Properties in Inflammatory Breast Cancer Cells by Manipulating the Endoplasmic Reticulum Stress PathwayFrontiers in oncologyJanuary 1, 2021Alsterda A, Asha K, Powrozek O, Repak M, Goswami S, Dunn AM, Memmel HC, Sharma-Walia N.Enhanced cell survival was determined using the CellTiter-fluor cell viability assay from Promega (#G6080) per the manufacturer’s instructions. This assay measures a conserved and constitutive protease activity

(17) Discovery of IPN60090, a Clinical Stage Selective Glutaminase-1 (GLS-1) Inhibitor with Excellent Pharmacokinetic and Physicochemical PropertiesJournal of Medicinal ChemistryNovember 12, 2020Michael J. Soth et al.microplate was incubated at 37 °C for 72 h. 10 μL of Promega’s CellTiter-Fluor reagent (Promega, Catalog #G6082), GF-AFC substrate diluted in assay buffer, was added to the plate for a 1x final concentration. 0.5%

(18) Development of a High-throughput Agar Colony Formation Assay to Identify Drug Candidates against MedulloblastomaPharmaceuticals (Basel)November 5, 2020Mohammed Sedeeq, Ahmed Maklad, Nuri Gueven, Iman AzimiRalph Stevenson, and was synthesised as previously described [28]. CellTiter-Fluor™ Cell Viability (G6081) was obtained from Promega (Madison, WA, USA). 4.2. Cell Culture D283 (HTB-185™) and D341 (HTB-187™)

(19) Plasmodium vivax Liver and Blood Stages Recruit the Druggable Host Membrane Channel Aquaporin-3Cell Chemical BiologyJune 18, 2020Dora Posfai, Steven P. Maher, Camille Roesch, Amélie Vantaux, Kayla Sylvester, Julie Péneau, Jean Popovici, Dennis E. Kyle, Benoît Witkowski, Emily R. Derbyshireof infected hepatocytes was measured 48 hpi by adding CellTiter-Fluor and Bright-Glo (Promega, Cat# G6080 and Cat# E2610) reagents, respectively. Fluorescence and luminescence signals were measured with an

(20) Bispecific antibodies enhance tumor-infiltrating T cell cytotoxicity against autologous HER-2-expressing high-grade ovarian tumorsJournal of leukocyte biologyJune 1, 2020Oberg HH, Janitschke L, Sulaj V, Weimer J, Gonnermann D, Hedemann N, Arnold N, Kabelitz D, Peipp M, Bauerschlag D, et al.determined as relative fluorescence units (RFU, 400Ex/505Em) using CellTiter‐Fluor Cell Viability Assay (#G6080, Promega, Mannheim, Germany). Therefore, a microplate‐reader (Infinite 200, Tecan, Crailsheim, Germany)

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CellTiter-Fluor Cell Viability Assay from Promega

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CellTiter-Fluor Cell Viability Assay from Promega

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