PURExpressIn Vitro Protein Synthesis Kit E6800L from New England Biolabs

Product Specs
ItemPURExpressIn Vitro Protein Synthesis Kit
CompanyNew England Biolabs
Price $2,466.00 Inquire
Catalog NumberE6800L
Quantity100 reactions
ApplicationsProteomics & Glycomics
Temperature-80 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equitySci AdvMay 29, 2026Severino Jefferson Ribeiro da Silva, Quinn Matthews, Séverine Cazaux, Justin R. J. Vigar, Bárbara N. R. Santos, Deyse C. M. Carvalho, Lauren A. Cranmer, David Duplat, Serena Singh, Mohammad Simchi, et al.toehold switch sensor was tested against its corresponding trigger elements using PURExpress (NEB, E6800L) according to the manufacturer’s protocols, as previously described (26, 34). Subsequently, the top-performing

(2) Genomic diversity analysis enables development of pan-Dengue Toehold RNA sensorsPLoS Negl Trop DisApril 16, 2026Anirudh Nandakumar, Deeksha Mishra, Akshay Shendre, Edrea Mendonca, Soujanya Nagendra, Akash Gulyani, Arati Rameshfrom Chakravarthy et al., 2021 [34]. The assay was conducted using the NEB PURExpress kit (Cat. no. E6800L). The reaction mixture consisted of 2 μl of solution A, 1.5 μl of solution B, 0.125 μl (10 Units) of

(3) ALS mutations disrupt self-association between the ubiquilin STI1 hydrophobic groove and internal placeholder sequencesThe EMBO JournalMarch 20, 2026Joan Onwunma, Saeed Binsabaan, Shawn P Allen, Sachini R Thanthirige, Deepika Gaur, Banumathi Sankaran, Matthew L Wohlever4010D X-ray Film Fixer,Fisher Scientific,Cat. # 4010 F PurExpress In Vitro protein synthesis kit,NEB,Cat. # E6800L isopropyl β-D-1-thiogalactopyranoside,RPI corp,Cat. # I56000-5.0 Phenylmethylsulfonyl fluoride,RPI

(4) Codebook: sequence specificity and genomic binding of poorly-characterized human transcription factorsbioRxivMarch 14, 2026Arttu Jolma, Kaitlin U. Laverty, Ali Fathi, Ally W.H. Yang, Isaac Yellan, Ilya E. Vorontsov, Sachi Inukai, Judith F. Kribelbauer-Swietek, Antoni J. Gralak, Rozita Razavi, et al.Doxycycline for 24 hours, used for inserts in pTH13195; (b) PURExpress T7 recombinant IVT system (NEB Cat.#E6800L), for inserts in pTH6838; or (c) SP6-driven wheat germ extract-based IVT (Promega Cat#L3260), for inserts

(5) Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessusAntimicrobial Agents and ChemotherapyMarch 2, 2026Tobias Funck, Kerry McGowen, Mark R. Sullivan, Samuel Zinga, Ian D. Wolf, Dennis Nurjadi, Claudia M. Denkinger, Eric J. Rubinin vitro protein synthesis kit were cloned into the manufacturer’s PURExpress DHFR Control Plasmid (E6800L, NEB) by swapping out DHFR with MAB_2736c (PCR amplified from ATC19977 gDNA) or mNeonGreen using standard

(6) GHT-SELEX demonstrates unexpectedly high intrinsic sequence specificity and complex DNA binding of many human transcription factorsbioRxivFebruary 24, 2026Arttu Jolma, Aldo Hernandez-Corchado, Ally W.H. Yang, Ali Fathi, Kaitlin U. Laverty, Alexander Brechalov, Rozita Razavi, Mihai Albu, Hong Zheng, The Codebook Consortium, et al.an in vitro transcription-translation reaction (PURExpress In Vitro Protein Synthesis Kit, NEB, Cat# E6800L) to express T7-driven, GST-tagged proteins (either full-length or DBDs) (plasmid backbone pTH683845).

(7) Toehold-VISTA: a machine learning approach to decipher programmable RNA sensor-target interactionsNucleic Acids ResFebruary 16, 2026James M Robson, Alexander A Greendescribed in manufacturer’s instructions. In vitro cell-free protein synthesis system (New England Biolabs E6800L) solutions A (0.4) and B (0.3) were combined, with the remaining volume composed of 0.005 U RNase inhibitor

(8) Click Detect: A Rapid and Sensitive Assay for Shiga Toxin 2 DetectionBiosensors (Basel)December 14, 2025Benjamin M. Thomas, Emma L. Webb, Katherine L. Yan, Alexi M. Fernandez, Zhilei Chenout exactly as described previously using the PURExpress In Vitro Protein Synthesis Kit (NEB, Cat# E6800L, Ipswich, MA, USA) with 500 ng of PCR amplified D#20 DNA as the template for each 12.5 µL transcription/translation

(9) Missense variants in human forkhead transcription factors reveal determinants of forkhead DNA bispecificityCell ReportsNovember 25, 2025Jessica King, Stephen S. Gisselbrecht, Julie-Alexia Dias, Raehoon Jeong, Elisabeth Rothman, Martha L. BulykHigh-Fidelity PCR MasterMix,Thermo Scientific,F531L PURExpress in vitro protein synthesis kit,New England Biolabs,E6800L 8 × 60K universal “all 10-mer” protein binding microarrays,Agilent Technologies,"GSE format, AMADID

(10) Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessusbioRxivOctober 28, 2025Tobias Funck, Kerry McGowen, Mark R. Sullivan, Samuel Zinga, Ian D. Wolf, Dennis Nurjadi, Claudia M. Denkinger, Eric J. Rubinin vitro protein synthesis kit were cloned into the manufacturer’s PURExpress DHFR Control Plasmid (E6800L, NEB) by swapping out DHFR with MAB_2736c (PCR amplified from ATC19977 gDNA) or mNeonGreen using standard

(11) Cotranslational folding and maturation of HIV-1 proteasebioRxivSeptember 2, 2025Justin Westerfield, Felix Nicolaus, Ronald Swanstrom, Gunnar von HeijneNew England BioLabs (Ipswich, Massachusetts, U.S.) PURExpress in vitro protein synthesis kit (catalog number E6800L). Briefly, 2.2 μL of linearized DNA was mixed with 0.8 μL [35S]-Met, 4 μL solution A and 3 μL solution

(12) A sticky situation – simple method for rapid poissonian encapsulation of highly aggregation-prone microbeads in polydisperse emulsionsFrontiers in Bioengineering and BiotechnologyJune 30, 2025Filip Hasecke, Wolfgang Hoyerin vitro transcription and translation reagent, PURExpress In Vitro Protein Synthesis Kit (catalog number: E6800L) was ordered from New England Biolabs. 3.2 Synthesis of b-FAM-A β -ATTO643 A precursor A β variant,

(13) Detection of TuMV by a toehold switch sensor coupled with NASBA amplification in Pseudostellaria heterophyllaPlant MethodsJune 9, 2025Yun Qiu, Shuxin Zhang, Aidi Tan, Qiang Cai, Kezhi Chen, Kaiwen Yang, Yanyang Jiao, Qiuting Lai, Chenjing Zhang, Sheng Lin, et al.(40%) and solution B (30%), the main reagents of the in vitro protein synthesis kit (PURExpress, NEB, E6800L), were mixed well with chlorophenol red-β-D-galactopyranoside (CPRG, Sigma‒Aldrich, 59767, 0.6 mg/mL)

(14) RNA toehold switch-based reporter assay to assess bacterial uptake of antisense oligomersmBioApril 9, 2025Paramita Sarkar, Linda Popella, Sandra Pérez-Jiménez, Jörg Vogelreporter translation in vitro, the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used according to the manufacturer’s instructions. In vitro transcribed RNA was prepared as described

(15) Widespread variation in molecular interactions and regulatory properties among transcription factor isoformsMolecular CellApril 3, 2025Luke Lambourne, Kaia Mattioli, Clarissa Santoso, Gloria Sheynkman, Sachi Inukai, Babita Kaundal, Anna Berenson, Kerstin Spirohn-Fitzgerald, Anukana Bhattacharjee, Elisabeth Rothman, et al.Human Coupled IVT Kit (for TBX5 isoforms) or PURExpress In Vitro Protein Synthesis Kit (for CREB1) (NEB E6800L), using the manufacturers’ recommended protocols, with the exception of an addition of 1.5 μL custom

(16) Quantifying protein unfolding kinetics with a high-throughput microfluidic platformbioRxivJanuary 18, 2025B. Atsavapranee, F. Sunden, D. Herschlag, P.M. Fordyceand expressed protein using the PURExpress expression system (New England Biolabs Inc., catalog no. E6800L). To prepare the expression mixture, we mixed 60 μL of PURExpress Solution A with 45 μL of Solution

(17) Mutations to transcription factor MAX allosterically increase DNA selectivity by altering folding and binding pathwaysNat CommunJanuary 14, 2025Renee Hastings, Arjun K. Aditham, Nicole DelRosso, Peter H. Suzuki, Polly M. Fordycethe device with PBS for 10 min. To express all TF variants simultaneously, we used PURExpress (NEB E6800L). Briefly, we equilibrated Parts A and B of PURExpress on ice until defrosted. For one device using

(18) HLA class II neoantigen presentation for CD4(+) T cell surveillance in HLA class II-negative colorectal cancerOncoimmunologyDecember 31, 2024Matsumoto S, Tsujikawa T, Tokita S, Mohamed Bedeir M, Matsuo K, Hata F, Hirohashi Y, Kanaseki T, Torigoe T.and translation (IVTT) was performed using the PURExpress In Vitro Protein Synthesis Kit (NEB, Cat# E6800L) according to the manufacturer’s instructions with 10 μg/μL template DNA and murine RNase inhibitor

(19) An in vitro protocol to assay mRNA translation inhibitors using the fluorescent assembly of the split-GFPSTAR ProtocolsDecember 20, 2024Ana Quiroz-Huanca, Ana Sanchez-Castro, Pablo Soriano-Castillo, Chiara Poletti, Therese Manuela Nloh Tientcheu, Attilio Fabbretti, Anna Maria Giuliodori, Pohl MilonColumn,Cytiva,Cat#28918778 PURExpress In vitro protein synthesis kit,New England Biolabs Inc.,Cat#E6800L PCR clean up Oligo Clean & Concentrator,Zymo Research,D4061 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, Primer

(20) NOVAsort for error-free droplet microfluidicsNature CommunicationsNovember 1, 2024Han Zhang, Rohit Gupte, Yuwen Li, Can Huang, Adrian R. Guzman, Jeong Jae Han, Haemin Jung, Rushant Sabnis, Paul de Figueiredo, Arum HanDNA template for the New England Biolabs (NEB) PURExpress in vitro protein synthesis reagent (NEB, #E6800L). Droplet generation (50 μm diameter) and droplet PCR amplification were carried out using the protocol

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PURExpressIn Vitro Protein Synthesis Kit

PURExpressIn Vitro Protein Synthesis Kit from New England Biolabs

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PURExpressIn Vitro Protein Synthesis Kit from New England Biolabs

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