NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) E7330L from New England Biolabs

Product Specs
ItemNEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible)
CompanyNew England Biolabs
Price $4,203.00 Inquire
Catalog NumberE7330L
Quantity96 reactions
ApplicationsDNA Analysis, Library Prep, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Profiling active RNA polymerase II transcription start sites from total RNA by capped small RNA sequencing (csRNA-seq)Nature ProtocolsJanuary 16, 2026Mackenzie K. Meyer, Oluwadamilola J. Olanrewaju, Patricia Montilla-Perez, Anna L. McDonald, Eva M. Rickard, Francesca Telese, Christopher Benner, Marina I. Savenkova, Sascha H. DuttkeNEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible), 96Rx (New England Biolabs, cat. no. E7330L) Barcodes: from NEBNext® Multiplex Small RNA Library Prep Kit for Illumina® Index Primers 1–48, (NEB,

(2) Transcription factors instruct DNA methylation patterns in plant reproductive tissuesNature Cell BiologyNovember 21, 2025Guanghui Xu, Yuhan Chen, Laura M. Martins, En Li, Fuxi Wang, Tulio Magana, Junlin Ruan, Julie A. Lawμg) was used for each smRNA library prep using the NEBNext Small RNA Library Prep Kit (NEB, cat. no. E7330L). The smRNA libraries were further purified using an 8% acrylamide gel and size selected (130–160 bp)

(3) Germ granule localization of nematode Argonaute WAGO-4 ensures fidelity in small RNA loadingThe EMBO JournalOctober 22, 2025Stela Jelenic, Mathias S Renaud, Samantha Del Borrello, Joseph Gokcezade, Janos Bindics, Lisa Frasz, Philipp Czermak, Peter Duchek, Julie M ClaycombRNA Library Prep,New England Biolabs,E7765 NEBNext Small RNA Library Prep Set for Illumina,New England Biolabs,E7330L Illumina NovaSeqX,Illumina, Illumina Miniseq,Illumina, Illumina Novaseq 6000,Illumina, Olympus IX83 inverted microscope

(4) A Near Telomere‐To‐Telomere Genome Assembly of Coffea arabica (Mundo Novo) Provides Insights Into Its Secondary MetabolismMolecular Ecology ResourcesSeptember 26, 2025Yi Liu, Hang Zong, Yaowu Xing, Xi Jiao, Zhuoya Liu, Yusheng Niu, Zhiling Yang, Shimeng Liu, Yongqiang Wang, Haodong Zhao, et al.was mixed and used to construct libraries using RNA library preparation kits (New England Biolabs, #E7330L). The libraries were subsequently sequenced on the Illumina platform (MGI). For Next‐generation genome

(5) Dynamic control of Argonautes by a rapidly evolving immunological switchCurrent BiologyJuly 7, 2025Chee Kiang Ewe, Guy Teichman, Shir Weiss, Maximilian M.L. Knott, Sarit Anava, Hila Gingold, Mario Bardan Sarmiento, Emily Troemel, Oded Rechaviassays,Critical commercial assays NEBNext® Small RNA Library Prep Set for Illumina®,New England Biolabs,Cat#E7330L NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®,New England Biolabs,"Cat#E7760, #E7765" High-Capacity

(6) Chloroplast precursor protein preClpD overaccumulation triggers multilevel reprogramming of gene expression and a heat shock-like responseNat CommunApril 22, 2025Zheng-Hui Hong, Liyu Zhu, Lin-Lin Gao, Zhe Zhu, Tong Su, Leonard Krall, Xu-Na Wu, Ralph Bock, Guo-Zhang Wulibraries were constructed using the NEBNext® Multiple Small RNA Library Prep Set for Illumina® (NEB, E7330L) and sequencing was performed on an Illumina HiSeq 2500 platform by Gene Denovo Biotechnology Co. (Guangzhou,

(7) Ultrafast bisulfite sequencing detection of 5-methylcytosine in DNA and RNANature BiotechnologyOctober 1, 2024Qing Dai, Chang Ye, Iryna Irkliyenko, Yiding Wang, Hui-Lung Sun, Yun Gao, Yushuai Liu, Alana Beadell, José Perea, Ajay Goel, et al.immediately on ice. Then 10 µl of 3′-ligation buffer and 3 µl of enzyme mix from NEB small RNA library kit (E7330L) were added and the mixture was incubated at 16 °C overnight. The excessive adapters were digested by

(8) Assessing Alzheimer's disease via plasma extracellular vesicle–derived mRNAAlzheimer's & Dementia: Diagnosis, Assessment & Disease MonitoringSeptember 14, 2024Le Hoang Phu Pham, Ching‐Fang Chang, Katherine Tuchez, Fei Liu, Yuchao ChenFC‐131‐1096). For fragmented mRNA within EVs, NEBNext Small RNA Library Prep Set (New England BioLabs, E7330L) was employed for library preparation according to manufacturer's instructions. Quality control of the

(9) SnoRNA profiling in colorectal cancer and assessment of non-invasive biomarker capacity by ddPCR in fecal samplesiScienceMarch 15, 2024Javier Gómez-Matas, Saray Duran-Sanchon, Juan José Lozano, Giulio Ferrero, Sonia Tarallo, Barbara Pardini, Alessio Naccarati, Antoni Castells, Meritxell GironellaKit,Illumina,Cat#RS-200-0012 NEBNext Multiplex Small RNA Library Prep for Illumina,New England Biolabs,Cat#E7330L QIAQuick PCR Purification Kit,QIAGEN,Cat#28104 TaqMan MicroRNA Reverse Transcription Kit,Life Technologies,Cat#4366596 TaqMan Preamp Master Mix

(10) Mapping the tRNA modification landscape of Bartonella henselae Houston I and Bartonella quintana ToulouseFront MicrobiolMarch 13, 2024Samia Quaiyum, Jingjing Sun, Virginie Marchand, Guangxin Sun, Colbie J. Reed, Yuri Motorin, Peter C. Dedon, Michael F. Minnick, Valérie de Crécy-LagardRNAs were then end-repaired and subjected to NEBNExt Small RNA Library Prep Set for Illumina (NEB, E7330L), according to manufacturer’s recommendations. Sequencing was performed in SR50 mode, with sequencing

(11) H3K36 methylation maintains cell identity by regulating opposing lineage programmesNature cell biologyAugust 1, 2023Hoetker MS, Yagi M, Di Stefano B, Langerman J, Cristea S, Wong LP, Huebner AJ, Charlton J, Deng W, Haggerty C, et al.reads per sample on average. Small RNA-seq libraries were generated using the NEB small RNA library kit E7330L (New England Biolabs), sequencing was run on the NextSeq 2000 as PE50 on a P3 type flowcell to ~30 million

(12) Small RNA shuffling between murine sperm and their cytoplasmic droplets during epididymal maturationDevelopmental CellMay 1, 2023Hetan Wang, Zhuqing Wang, Tong Zhou, Dayton Morris, Sheng Chen, Musheng Li, Yue Wang, Huili Zheng, Weineng Fu, Wei Yanconstructed using NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) (NEB, Cat#E7330L) according to the manufacturer’s instructions, as described previously58. Duplicate samples were used

(13) miR‑30c reduces myocardial ischemia/reperfusion injury by targeting SOX9 and suppressing pyroptosisExperimental and Therapeutic MedicineMarch 10, 2023Jia Nie, Wenjing Zhou, Shouyang Yu, Song Cao, Haiying Wang, Tian Yusmall RNA libraries using the NEB Next Multiplex Small RNA Library Prep Set for Illumina (catalog # E7330L, New England Biolabs, Inc.), index codes were added to attribute sequences for each sample. Agilent

(14) Culex Mosquito Piwi4 Is Antiviral against Two Negative-Sense RNA VirusesVirusesDecember 10, 2022Elizabeth Walsh, Tran Zen B. Torres, Claudia Rückertlibraries were prepared using the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs, #E7330L, Ipswich, MA, USA), following the manufacturer’s protocol. The 3′ adaptor was ligated for 18 h at 16

(15) MicroRNA-4516 in Urinary Exosomes as a Biomarker of Premature Ovarian InsufficiencyCellsSeptember 7, 2022Umair Z, Baek MO, Song J, An S, Chon SJ, Yoon MS.Korea). A microRNA library was constructed by using the NEB Next Multiplex Small RNA Library Prep Kit (E7330L, New England BioLabs, Inc., Beverly, MA, USA) according to the manufacturer’s instructions. Briefly,

(16) Intramolecular ligation method (iLIME) for pre-miRNA quantification and sequencingRNAJuly 1, 2022Minh Ngoc Le, Cong Truc Le, Tuan Anh NguyenSmall RNA Library Preparation Kit (Lau and Lee 2012), the NEBNext Small RNA Library Prep Set (NEB, E7330L); and the SMARTer smRNA Kit (Takara, 635031). However, pre-miRNA sequencing is complex since ligating

(17) Ablation of the miR-465 Cluster Causes a Skewed Sex Ratio in MiceFront Endocrinol (Lausanne)May 23, 2022Zhuqing Wang et al.Small RNA libraries were constructed using NEBNext® Small RNA Library Prep Set for Illumina® (Cat. # E7330L, NEB) according to the manufacturer’s instructions. miRNA qPCR was performed as described (14). All

(18) Perinatal Exposure to Nicotine Alters Sperm RNA Profiles in RatsFront Endocrinol (Lausanne)May 4, 2022Hetan Wang, Jie Liu, Jianjun Gao, Wei Yan, Virender K. Rehanconstructed using NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible) (NEB, Cat No. E7330L) according to the manufacturer’s instructions, as described previously (43), and sequenced using HiSeq

(19) Discovery and Verification of Extracellular miRNA Biomarkers for Non-invasive Prediction of Pre-eclampsia in Asymptomatic WomenCell Reports MedicineAugust 31, 2020Srimeenakshi Srinivasan et al.,Agilent Technologies,5067-4626 NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible),New England Biolabs,E7330L ,, Deposited Data,Deposited Data,Deposited Data ,, "Small RNA-seq data, miRNA",This paper,"See https://www.ncbi.nlm.nih.gov/gap/,

(20) Control of Early B Cell Development by the RNA N⁶-Methyladenosine MethylationCell reportsJune 30, 2020Zheng Z, Zhang L, Cui XL, Yu X, Hsu PJ, Lyu R, Tan H, Mandal M, Zhang M, Sun HL, et al.ZR small-RNA PAGE Recovery Kit,Zymo Research,Cat#: R1070 NEBNext Small RNA Library Prep Set for Illumina,New England Biolabs,Cat#: E7330L Deposited Data,Deposited Data,Deposited Data "Raw data: RNA-seq, ATAC-seq and ribosome profiling of

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NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible)

NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) from New England Biolabs

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NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) from New England Biolabs

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