NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity declineJ Anim Sci BiotechnolApril 22, 2026Mingyue Gao, Junnan Zhang, Boxuan Zhang, Xinwei Jiang, Bowen Niu, Conghao Zhong, Fangren Lan, Wenxin Zhang, Ning Yang, Congjiao Sunmagnesium-based fragmentation buffer (NEBNext® Magnesium RNA Fragmentation Module, New England Biolabs, Cat. No. E6150S). First-strand cDNA synthesis was performed using SuperScript™ II Reverse Transcriptase (Invitrogen,

(2) IGF2BP3-mediated stabilization of RHOA mRNA promotes osteosarcoma proliferation via Hippo signalingTranslational Cancer ResearchMarch 31, 2026Jingbin Wu, Jiahao Xia, Yuan Tao, Yiping Wengto controlled fragmentation through treatment with RNA Fragmentation Reagent (NEB, Beverly, MA, USA; E6150S). Prior to immunoprecipitation, Protein G magnetic beads were prepared by incubating them with anti-N6-methyladenosine (m6A

(3) The bladder cancer m6A landscape is defined by global methylation dilution and focal 3′-UTR hypermethylationEMBO ReportsMarch 23, 2026Jonas Koch, Jinyun Xu, Felix Bormann, Vitor Coutinho Carneiro, Manuel Neuberger, Katja Nitschke, Malin Nientiedt, Philipp Erben, Maurice Stephan Michel, Manuel Rodriguez-Paredes, et al.Kit,Thermo Fisher Scientific,Cat 61006 NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,Cat E6150S RNA Clean & Concentrator-5 kit (DNase I included),Zymo Research,Cat R1013 Glyoxal solution,Sigma-Aldrich,Cat

(4) LENG8 mediates RNA nuclear retention and degradation in eukaryotesMol CellMarch 19, 2026Lusong Tian, Liang Liu, Yoseop Yoon, Lindsey V. Soles, Marielle Valdez, Joshua Jeong, Clinton Yu, Lan Huang, Yongsheng ShiMix,Takara,Cat#638949 Blasticidin,Invivogen,Cat#ant-bl-05 Puromycin,Invivogen,Cat#ant-pr-1 dTAGv-1,Tocris,Cat#6914 Polybrene,Sigma,Cat#TR-1003 Trizol,Invitrogen,Cat#15596026 NEBNext Magnesium RNA fragmentation module,NEB,Cat#E6150S Phusion High-Fidelity PCR Master Mix with HF Buffer,NEB,Cat#M0531L Lonza SF Cell Line 4D-Nucleofector,ThermoFisher,Cat#NC0281111 X

(5) ABA-Induced Transcriptomic Dynamics in Arabidopsis thaliana Anthers: Insights into Pollen Development and FertilityPlants (Basel)March 13, 2026Lu Liu, Huiting Huang, Dexi Shi, Shuo Wang, Ziyi Lin, Fengming Huang, Li Huang, Sue Linusing NEBNext® Magnesium RNA Fragmentation Module (New England Biolabs, Ipswich, MA, USA, Cat. No. E6150S) followed by ribosomal RNA depletion with the Ribo-Zero Gold rRNA Removal Kit (Illumina, San Diego,

(6) Molecular mechanism of gallium nitrate in inhibiting bacterial biofilm formation through pykF modulationPLoS OneMarch 6, 2026Xiaofeng Zhang, Junjie Dong, Bing Wang, Lingqiang Chen, Zhiqiang Gong, Jin Yang, Guizhao Shu, Qi NingmRNA was then fragmented at 94°C for 5–7 min using the NEBNext Magnesium RNA Fragmentation Module (E6150S; New England Biolabs, USA). The fragmented RNA was reverse transcribed into cDNA using SuperScript II Reverse Transcriptase

(7) Stress fiber traction force reshapes chromatin accessibility and YAP binding to direct diverse transcriptional programs in mesenchymal stem cellsMechanobiol MedMarch 4, 2026Jiacheng Lei, Yicen Long, Xiaojing Liu, Zhiqin Chu, Qiang Weimagnesium ion-based RNA fragmentation module (NEBNext Magnesium RNA Fragmentation Module, NEB, cat.E6150S, USA) at 94 °C for 5-7 min. First-strand cDNA was synthesized using SuperScript™ II Reverse Transcriptase

(8) Comprehensive Profiling of N 6 ‐methyladnosine (m 6 A) Readouts Reveals Novel m 6 A Readers That Regulate Human Embryonic Stem Cell DifferentiationAdvanced ScienceMarch 1, 2026Zhou Huang, Rucong Liu, Zibaguli Wubulikasimu, Wanqing Zhao, Jiaqi Huang, Jiaxuan Wang, Tianyuan Zhang, Rui Fan, Wei Kong, Qinghua Cui, et al.purification kit (Thermo fisher, catalog#AM1908). RNA was incubated with fragmentation buffer (NEB, catalog#E6150S) for 4 min at 94°C and glyoxal solution and DMSO were added, and the mixture was incubated for 30 min

(9) RBM15B recognizes H3K79me2 to guide selective m6A-modification of mRNA and enhance oncoprotein translation in MLL-r leukemiaThe EMBO JournalFebruary 2, 2026Tian-Qi Chen, Yu-Meng Sun, Shun-Xin Zhu, Xiao-Tong Chen, Ke-Jia Pu, Heng-Jing Huang, Qi Pan, Jun-Yi Lian, Wei Huang, Ke Fang, et al.Dynabeads Oligo(dT)25 (Promega, Z5300). mRNA was fragmented by incubation at 94 °C for 4 min with fragment buffer (NEB, E6150S). Two hundred nanograms of fragmented RNA were ordered through RNA protection, deamination, and the

(10) Targeting DAP5 Disrupts Alternate Mode of Translational Initiation in Tregs and Potentiates Antitumor ImmunityAdvanced ScienceFebruary 1, 2026Xiaojiang Lai, Guihong Pan, Xixi Feng, Jun Zhang, Yunxia Li, Shanmeizi Zhao, Zhenming Lin, Yun Feng, Wei Bao, Hongyan Yang, et al.RNAs were fragmented into ≈200 bp pieces with the NEBNext Magnesium RNA Fragmentation Module (NEB, Cat#E6150S) and purified with the Monarch RNA Cleanup Kit (NEB, #T2040L). The purified RNA fragments were dissolved

(11) Investigation of myofiber composition changes and molecular mechanisms in rabbit meat quality development during growthBMC GenomicsJanuary 31, 2026Guohua Song, TongYan Zhu, Zhen Zhen, LiYe Chang, Ahamba Ifeanyi Solomon, Jie Jie, ShuHui Wang, Song Bing, XiangGui Dong, ZhanJun Renshort fragments at high temperature using the NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. E6150S, USA) and subsequently reverse transcribed to generate six libraries for mRNA, lncRNA, and circRNA.

(12) Evaluation of Artemisia annua-Scutellaria baicalensis Herb Pair Carbon Dots Interfering NLRP3 Inflammatory Pathway For Effective Against H1N1 Viral Pneumonia in Mice ModelInternational Journal of NanomedicineJanuary 23, 2026Qianyun Zhang, Xinyi Huang, Huimei Kong, Qiongyao Liang, Xiaoqing Lv, Yuan Ma, Jingbo Wang, Jing Chenhigh-temperature fragmentation using the NEBNext® Magnesium RNA Fragmentation Module (NEB, Cat. No. E6150S, USA) at 94 °C for 5–7 min. The fragmented RNA was used for cDNA synthesis using SuperScriptTM II Reverse

(13) CAR-T manufacturing reduces heterogeneity between CIDP and multiple myeloma patient-derived T cellsTheranosticsJanuary 22, 2026Xu Wang, Pu Wang, Qi Zhou, Xianzheng Wei, Yuhang Jin, Ying Liao, Xuan Zhao, Rui Hou, Sijin Li, Zhangchun Guan, et al.cat#25-61005) for mRNA enrichment. Libraries were constructed via magnesium fragmentation (NEB, cat#E6150S), cDNA synthesis, and end repair, yielding 300 ± 50 bp fragments. Paired-end 150 bp sequencing was conducted

(14) An Integrative Small RNA–Degradome–Transcriptome Analysis Reveals Mechanisms of Heat-Induced Anther Indehiscence in PepperBiology (Basel)January 12, 2026Gang Lei, Tao Li, Kunhua Zhou, Xinjie Yuan, Yueqin Huang, Gege Li, Yu Fang, Rong Fang, Xuejun Chenpurifying, the mRNA was fragmented into small pieces via the Magnesium RNA Fragmentation Module (NEB, cat. E6150S, Ipswich, MA, USA). Then, the cleaved mRNA fragments were used to form the final cDNA library according

(15) Vagus nerve stimulation alleviates S. aureus-induced mastitis by regulating gut microbiota S24-7-PPARγ and NF-ΚB/NLRP3 signaling in miceJournal of NeuroinflammationDecember 23, 2025Yuhong He, Yue Zhang, Lihua Zhao, Zeming Zhou, Nier Su, Can Zhang, Keyi Wang, Lei Jin, Bin Yang, Xiaoyu Hu, et al.mRNA was fragmented (94°C, 5–7 min) employing the NEBNext® Magnesium RNA Fragmentation Module (NEB, E6150S). First-strand cDNA synthesis utilized SuperScript™ II Reverse Transcriptase (Invitrogen, 1896649) with

(16) Polymorphism-driven transcriptomic changes in anthelmintic metabolism pathways of Anisakis simplex s.s. L3 larvaeParasit VectorsDecember 19, 2025Mateusz Maździarz, Iwona Polak, Lukasz Paukszto, Monika Szczecińska, Elżbieta Łopieńska-Biernatmanufacturer’s instructions. After removal of the ribosomal RNAs, the remaining RNAs were fragmented (Cat. No. E6150S; NEBNext® Magnesium RNA Fragmentation Module, New England Biolabs, Ipswich, MA, USA) into short fragments

(17) CX3CL1 deficiency ameliorates acute kidney injury by inhibiting macrophage mitochondrial dysfunction and mtDNA-cGAS-STING signalingCell Death DiscoveryDecember 13, 2025Qiming Gong, Fahui Liu, Yuqing Huang, Dehui Li, Tingting Zhou, Chen Zeng, Yan Jiang, Huang Wei, Yong Xu(Thermo Fisher, 25-61005) and fragmented with the NEBNext Magnesium RNA Fragmentation Module (NEB, E6150S) at 94 °C. First-strand cDNA synthesis was performed using SuperScript II (Invitrogen, 1896649), followed

(18) Nuclear PD-L1 drives IFN-γ-promoted lung metastasis of triple-negative breast cancer via POLR2A-mediated transcriptional activation of LY6EBreast Cancer ResearchDecember 12, 2025Xu Wang, Qi Zhou, Pu Wang, Shunshun Bao, Xianzheng Wei, Xuan Zhao, Rui Hou, Sijin Li, Zhangchun Guan, Wen Ma, et al.#25-61005) for mRNA enrichment. Libraries were constructed via magnesium-mediated fragmentation (NEB, #E6150S), cDNA synthesis, and end repair, yielding 300 ± 50 bp fragments [27]. PE150 sequencing was performed

(19) Epstein-Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1-TSC22D1 AxisMicroorganismsDecember 11, 2025Yea Rim An, Jaehun Jung, Kyeong Min Kwon, Jun Yeob Kim, Min-Hyeok Lee, Ju Yeon Lee, Minho Lee, Suk Kyeong LeemRNA was fragmented into 100–200 nt fragments using the NEBNext Magnesium RNA Fragmentation Module (#E6150S, New England Biolabs, Ipswich, MA, USA) and subsequently purified using the Monarch RNA Cleanup Kit

(20) Molecular Network Analysis of HBV Persistent Infection from the Perspective of Whole TranscriptomeBiomoleculesDecember 1, 2025Qiuping Chen, Congying Tang, Haiyang Hu, Yichen Peng, Jibin Liu, Peijie Wu, Quansheng Feng, Yuming Jiang, Baixue LiCA, USA). Subsequently, fragment the RNA using the NEBNext® Magnesium RNA Fragmentation Module (NEB, E6150S, Ipswich, MA, USA) under high temperature and divalent cation conditions. Using SuperScript™ II reverse transcriptase

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NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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