NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) RBM15B recognizes H3K79me2 to guide selective m6A-modification of mRNA and enhance oncoprotein translation in MLL-r leukemiaThe EMBO JournalFebruary 2, 2026Tian-Qi Chen, Yu-Meng Sun, Shun-Xin Zhu, Xiao-Tong Chen, Ke-Jia Pu, Heng-Jing Huang, Qi Pan, Jun-Yi Lian, Wei Huang, Ke Fang, et al.Dynabeads Oligo(dT)25 (Promega, Z5300). mRNA was fragmented by incubation at 94 °C for 4 min with fragment buffer (NEB, E6150S). Two hundred nanograms of fragmented RNA were ordered through RNA protection, deamination, and the

(22) Targeting DAP5 Disrupts Alternate Mode of Translational Initiation in Tregs and Potentiates Antitumor ImmunityAdvanced ScienceFebruary 1, 2026Xiaojiang Lai, Guihong Pan, Xixi Feng, Jun Zhang, Yunxia Li, Shanmeizi Zhao, Zhenming Lin, Yun Feng, Wei Bao, Hongyan Yang, et al.RNAs were fragmented into ≈200 bp pieces with the NEBNext Magnesium RNA Fragmentation Module (NEB, Cat#E6150S) and purified with the Monarch RNA Cleanup Kit (NEB, #T2040L). The purified RNA fragments were dissolved

(23) Investigation of myofiber composition changes and molecular mechanisms in rabbit meat quality development during growthBMC GenomicsJanuary 31, 2026Guohua Song, TongYan Zhu, Zhen Zhen, LiYe Chang, Ahamba Ifeanyi Solomon, Jie Jie, ShuHui Wang, Song Bing, XiangGui Dong, ZhanJun Renshort fragments at high temperature using the NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. E6150S, USA) and subsequently reverse transcribed to generate six libraries for mRNA, lncRNA, and circRNA.

(24) Evaluation of Artemisia annua-Scutellaria baicalensis Herb Pair Carbon Dots Interfering NLRP3 Inflammatory Pathway For Effective Against H1N1 Viral Pneumonia in Mice ModelInternational Journal of NanomedicineJanuary 23, 2026Qianyun Zhang, Xinyi Huang, Huimei Kong, Qiongyao Liang, Xiaoqing Lv, Yuan Ma, Jingbo Wang, Jing Chenhigh-temperature fragmentation using the NEBNext® Magnesium RNA Fragmentation Module (NEB, Cat. No. E6150S, USA) at 94 °C for 5–7 min. The fragmented RNA was used for cDNA synthesis using SuperScriptTM II Reverse

(25) Evaluation of Artemisia annua-Scutellaria baicalensis Herb Pair Carbon Dots Interfering NLRP3 Inflammatory Pathway For Effective Against H1N1 Viral Pneumonia in Mice ModelInt J NanomedicineJanuary 23, 2026Qianyun Zhang, Xinyi Huang, Huimei Kong, Qiongyao Liang, Xiaoqing Lv, Yuan Ma, Jingbo Wang, Jing Chenhigh-temperature fragmentation using the NEBNext® Magnesium RNA Fragmentation Module (NEB, Cat. No. E6150S, USA) at 94 °C for 5–7 min. The fragmented RNA was used for cDNA synthesis using SuperScriptTM II Reverse

(26) BRD2 bridges TFIID and MOF-H4K16ac-containing nucleosomes to promote transcriptional initiationMolecular CellJanuary 22, 2026Bin Zheng, Ruxuan Qiu, Sarah Gold, Marta Iwanaszko, Yuki Aoi, Benjamin Charles Howard, Madhurima Das, Ali Shilatifardfrom Drosophila S2 was added. Mixed RNA was fragmented using Magnesium RNA Fragmentation Module (NEB, #E6150S), followed by biotinylation of 4sU using Biotin-XX-MTSEA (Biotium, #90066) in 20% DMF. Biotinylated

(27) CAR-T manufacturing reduces heterogeneity between CIDP and multiple myeloma patient-derived T cellsTheranosticsJanuary 22, 2026Xu Wang, Pu Wang, Qi Zhou, Xianzheng Wei, Yuhang Jin, Ying Liao, Xuan Zhao, Rui Hou, Sijin Li, Zhangchun Guan, et al.cat#25-61005) for mRNA enrichment. Libraries were constructed via magnesium fragmentation (NEB, cat#E6150S), cDNA synthesis, and end repair, yielding 300 ± 50 bp fragments. Paired-end 150 bp sequencing was conducted

(28) An Integrative Small RNA–Degradome–Transcriptome Analysis Reveals Mechanisms of Heat-Induced Anther Indehiscence in PepperBiology (Basel)January 12, 2026Gang Lei, Tao Li, Kunhua Zhou, Xinjie Yuan, Yueqin Huang, Gege Li, Yu Fang, Rong Fang, Xuejun Chenpurifying, the mRNA was fragmented into small pieces via the Magnesium RNA Fragmentation Module (NEB, cat. E6150S, Ipswich, MA, USA). Then, the cleaved mRNA fragments were used to form the final cDNA library according

(29) Vagus nerve stimulation alleviates S. aureus-induced mastitis by regulating gut microbiota S24-7-PPARγ and NF-ΚB/NLRP3 signaling in miceJournal of NeuroinflammationDecember 23, 2025Yuhong He, Yue Zhang, Lihua Zhao, Zeming Zhou, Nier Su, Can Zhang, Keyi Wang, Lei Jin, Bin Yang, Xiaoyu Hu, et al.mRNA was fragmented (94°C, 5–7 min) employing the NEBNext® Magnesium RNA Fragmentation Module (NEB, E6150S). First-strand cDNA synthesis utilized SuperScript™ II Reverse Transcriptase (Invitrogen, 1896649) with

(30) Polymorphism-driven transcriptomic changes in anthelmintic metabolism pathways of Anisakis simplex s.s. L3 larvaeParasit VectorsDecember 19, 2025Mateusz Maździarz, Iwona Polak, Lukasz Paukszto, Monika Szczecińska, Elżbieta Łopieńska-Biernatmanufacturer’s instructions. After removal of the ribosomal RNAs, the remaining RNAs were fragmented (Cat. No. E6150S; NEBNext® Magnesium RNA Fragmentation Module, New England Biolabs, Ipswich, MA, USA) into short fragments

(31) CX3CL1 deficiency ameliorates acute kidney injury by inhibiting macrophage mitochondrial dysfunction and mtDNA-cGAS-STING signalingCell Death DiscoveryDecember 13, 2025Qiming Gong, Fahui Liu, Yuqing Huang, Dehui Li, Tingting Zhou, Chen Zeng, Yan Jiang, Huang Wei, Yong Xu(Thermo Fisher, 25-61005) and fragmented with the NEBNext Magnesium RNA Fragmentation Module (NEB, E6150S) at 94 °C. First-strand cDNA synthesis was performed using SuperScript II (Invitrogen, 1896649), followed

(32) Nuclear PD-L1 drives IFN-γ-promoted lung metastasis of triple-negative breast cancer via POLR2A-mediated transcriptional activation of LY6EBreast Cancer ResearchDecember 12, 2025Xu Wang, Qi Zhou, Pu Wang, Shunshun Bao, Xianzheng Wei, Xuan Zhao, Rui Hou, Sijin Li, Zhangchun Guan, Wen Ma, et al.#25-61005) for mRNA enrichment. Libraries were constructed via magnesium-mediated fragmentation (NEB, #E6150S), cDNA synthesis, and end repair, yielding 300 ± 50 bp fragments [27]. PE150 sequencing was performed

(33) Epstein-Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1-TSC22D1 AxisMicroorganismsDecember 11, 2025Yea Rim An, Jaehun Jung, Kyeong Min Kwon, Jun Yeob Kim, Min-Hyeok Lee, Ju Yeon Lee, Minho Lee, Suk Kyeong LeemRNA was fragmented into 100–200 nt fragments using the NEBNext Magnesium RNA Fragmentation Module (#E6150S, New England Biolabs, Ipswich, MA, USA) and subsequently purified using the Monarch RNA Cleanup Kit

(34) Molecular Network Analysis of HBV Persistent Infection from the Perspective of Whole TranscriptomeBiomoleculesDecember 1, 2025Qiuping Chen, Congying Tang, Haiyang Hu, Yichen Peng, Jibin Liu, Peijie Wu, Quansheng Feng, Yuming Jiang, Baixue LiCA, USA). Subsequently, fragment the RNA using the NEBNext® Magnesium RNA Fragmentation Module (NEB, E6150S, Ipswich, MA, USA) under high temperature and divalent cation conditions. Using SuperScript™ II reverse transcriptase

(35) SARS-CoV-2 and HCoV-OC43 regulate host m6A modification via activation of the mTORC1 signalling pathway to facilitate viral replicationEmerging microbes & infectionsDecember 1, 2025Zhou S, Hui X, Wang W, Zhao C, Jin M, Qin Y, Chen M.(Thermo Fisher). The mRNA was then fragmented using the NEBNext® Magnesium RNA Fragmentation Module (NEB, E6150S) at 86°C for 7 min according to the manufacturer’s instructions, with 10% of the mRNA saved as input.

(36) Targeting Fibrotic Scarring by Mechanoregulation of Il11ra1 + /Itga11 + Fibroblast Patterning Promotes Axon Growth after Spinal Cord InjuryAdvanced ScienceNovember 1, 2025Longyou Xiao, Kaixi Shi, Wen Li, Jialin Liu, Pengfei Xie, Zijun Hu, Yu Dai, Haiyan Weng, Qiuju Yuan, Wutian Wu, et al.Fragmentation was performed at 94 °C for 5–7 min using the NEBNext Magnesium RNA Fragmentation Module (E6150S, NEB, USA). First‐strand cDNA synthesis was performed using SuperScript II (1 896 649, Thermo Fisher

(37) Arsenic regulates ALKBH1 abundance and substrate specificity to promote translation and tumorigenicityCell ReportsOctober 28, 2025Emma Wilkinson, Yan-Hong Cui, Zizhao Yang, Michelle Verghese, Seungwon Yang, Ming Sun, Houxiang Zhu, Jack Peterson, Jiangbo Wei, Yingming Zhao, et al.61012). 1 μg of mRNA was fragmented to ∼200 nt using the NEBNext Magnesium RNA Fragmentation Module (NEB, E6150S) according to the manufacturer’s instructions. 5% of the fragmented mRNA was saved as input. mRNA fragments

(38) Prognostic relevance of PRSS2 and its immune correlates in papillary thyroid carcinomaOpen Med (Wars)October 23, 2025Wei Lin, Linwen Zeng, Xiaoxiao Jiang, Xiangdong Kong, Jianming Gong, Ming Wufollowed by fragmentation with the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs, E6150S). cDNA was generated from the fragmented RNA and amplified to construct sequencing libraries with an

(39) Concentration-dependent formation of intersegment interactions in the viral inclusions of influenza A virus infected cellsiScienceOctober 17, 2025Naoki Takizawa, Koichi Higashi, Risa Karakida Kawaguchi, Yasuhiro Gotoh, Tetsuya Hayashi, Ken Kurokawahydrochloride,Sigma-Aldrich,A4330-5MG ISOGEN II,NIPPON GENE,311-07361 NEBNext Magnesium RNA Fragmentation Module,New England Biolabs,E6150S CIAP,Takara Bio,2250A T4 polynucleotide kinase,Toyobo,PNK-111 ReverTra Ace,Toyobo,TRT-101 Taq polymerase,Roche,11146165001 Dynabeads MyOne Streptavidin C1

(40) RNA m1A methyltransferase TRMT61A promotes colorectal tumorigenesis by enhancing ONECUT2 mRNA stability and is a potential therapeutic targetCancer CommunicationsOctober 16, 2025Xiaoting Zhang, Na Qin, Fenfen Ji, Hao Su, Haiyun Shang, Hongyan Chen, Dan Huang, Qing Li, Jing Ren, Weixin Liu, et al.fragmented into ∼200 bp pieces with the New England Biolabs Next® Magnesium RNA Fragmentation Module (#E6150S, New England Biolabs) and then purified with Ethanol precipitation. One‐tenth of purified RNAs were

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