NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) E7330S from New England Biolabs

Product Specs
ItemNEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible)
CompanyNew England Biolabs
Price $1,236.00 Inquire
Catalog NumberE7330S
Quantity24 reactions
ApplicationsDNA Analysis, Library Prep, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Quantitative profiling of RNA modifications enriched in non-membrane-bound cellular structures using APEX-RNA-MSCell Chemical BiologyJune 22, 2026Kyung W. Seo, Dhruv Y. Dhingani, Ralph E. Kleinerprotocols47,48. RNA-seq libraries were generated with NEBNext Small RNA Library Prep kit for Illumina (NEB, E7330S), and amplified cDNAs were submitted for Illumina sequencing at the Princeton Genomics Core Facility.

(2) Platelet-rich plasma cues program M2 macrophage–derived exosome mimetics to regenerate the tendon–bone interfaceJournal of NanobiotechnologyJune 2, 2026Chihao Lin, Hongyi Jiang, Linjie Chen, Liang Zhu, Yuhan Wang, Hanting Shen, Zhongnan Lin, Junfeng Shi, Jilong Wang, Junjie Deng, et al.RNA per sample was processed with the NEBNext Small RNA Library Prep Set for Illumina (Cat. No. NEB#E7330S, NEB, USA) as recommended. The procedure involved ligating adapters to both ends of the RNA molecules,

(3) Structural and biochemical characterization of yeast Tcd enzymes installing the post-transcriptional modification ct6A in tRNANucleic Acids ResMay 5, 2026Julia Hirschmann, Rachel Sonntag, Matthias Heiss, Ewa Wegrzyn, Wolfgang Heinemeyer, Thomas Carell, Eva M Huberfragments were converted to library using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB ref. E7330S, USA) following the manufacturer’s recommendations. DNA library was quantified using a fluorometer (Qubit 2.0 fluorometer

(4) A trans -piRNA network and transcriptional antagonism shape piRNA cluster functionbioRxivApril 9, 2026Yicheng Luo, Daniel Siqueira Siqueira de Oliveira, Peng He, Alexei A. Aravinand purified. Small RNA libraries were generated using the NEBNext Small RNA Library Prep Kit (NEB #E7330S) and sequenced on an Illumina HiSeq 2500 platform. RT-qPCR Approximately 20 ovaries were dissected

(5) The bladder cancer m6A landscape is defined by global methylation dilution and focal 3′-UTR hypermethylationEMBO ReportsMarch 23, 2026Jonas Koch, Jinyun Xu, Felix Bormann, Vitor Coutinho Carneiro, Manuel Neuberger, Katja Nitschke, Malin Nientiedt, Philipp Erben, Maurice Stephan Michel, Manuel Rodriguez-Paredes, et al.,New England Biolabs,Cat M0201S NEBNext Small RNA Library Prep Set for Illumina,New England Biolabs,Cat E7330S NEBNext Multiplex Oligos for Illumina (Index Primer Sets 1 and 3),New England Biolabs,Cat E7335S and

(6) Pseudouridine selects RNAs for extracellular transportbioRxivMarch 8, 2026Alessandro Scacchetti, Tuan D. Tran, Emily J. Shields, Lauren N. Reich, John F. Doherty, Julia A. Tasca, Grace E. Lee, Alejandro A. Vilcaes, Richard Lauman, Natali L. Chanaday, et al.libraries were prepared using the NEBnext small RNA library kit for Illumina (New England Biolabs, Cat. No E7330S), following the standard protocol with the following parameters: 1) 1:2 dilution of 3’SR Adaptor, SR

(7) DICER cleavage fidelity is governed by 5′-end binding pocketsNatureMarch 4, 2026Minh Khoa Ngo, Cong Truc Le, Tuan Anh Nguyengel. Library preparation was performed using the NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S). In brief, the purified small RNA fragments were first ligated to an adenylated 3′ adapter (AppAGATCGGAAGAGCACACGTCT-NH2).

(8) Functional characterization of the 9q34.13 locus identifies RAPGEF1 as modulating risk for melanoma and nevi via RAS activationbioRxivFebruary 12, 2026Rohit Thakur, Mai Xu, Alexandra M. Thornock, Joshuah Yon, Samuel Anyaso-Samuel, Martin Lauss, Thomas Rehling, Linh Bui-Raborn, Hayley Sowards, Gerard Duncan, et al.instruction. miRNA sequencing libraries were constructed using NEB Next Small RNA Library Prep kit (E7330s) and the sequencing was performed on HiSeq2500. All samples show the proportion of Q30 or higher bases

(9) Joint transcriptome-wide and proteomic analysis of the mechanisms underlying the regulation of sheep wool fiber diameterAnimal BioscienceFebruary 1, 2026Lin Yue, Zengkui Lu, Tingting Guo, Bowen Chen, Jianbin Liu, Chao Yuanconstructed, purified, and sequenced using the NEBNext Small RNA Library Prep Set for Illumina kit (NEB#E7330S; NEB). Filtering of low quality reads, The length distribution of the clean sequences in the reference

(10) Conserved shifts in sperm small non-coding RNA profiles during mouse and human agingThe EMBO JournalJanuary 20, 2026Junchao Shi, Xudong Zhang, Chen Cai, Shichao Liu, Jiancheng Yu, Emma R James, Lihua Liu, Benjamin R Emery, Megan R McMurray Bires, Elizabeth Torres-Arce, et al.sourced from the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs, Catalog No. E7330S) and were ligated in sequence. First, a 3′ adapter was added under the following reaction conditions:

(11) Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domainsMolecular CellJanuary 8, 2026Lin Guo, Jacob R. Mann, Jocelyn C. Mauna, Katie E. Copley, Hejia Wang, Jack D. Rubien, Cristian A. Bergmann, Jenny L. Carey, Jessica Merjane, Marilyn Ngo, et al.QuikChange Site-Directed Mutagenesis Kit,Agilent,Cat# 210518 NEBNext® Small RNA Library Prep Set for Illumina®,New England Biolabs,Cat# E7330S Deposited data,, RNA sequencing data,Gene Expression Omnibus,GSE249059.Token:irkdymqknvihfkv "Gel,

(12) Paternal Microplastic Exposure Alters Sperm Small Noncoding RNAs and Affects Offspring Metabolic Health in MiceJournal of the Endocrine SocietyJanuary 7, 2026Seung Hyun Park, Jianfei Pan, Xudong Zhang, Ting-An Lin, Sijie Tang, Xiuchun Li, Sihem Cheloufi, Qi Chen, Tong Zhou, Changcheng ZhouRNase inhibitor). TRIzol Reagent was used again for RNA purification. Adapters (New England Biolabs, E7330S) were ligated to the purified RNA, followed by first-strand cDNA synthesis and library preparation.

(13) Identification of competing endogenous RNA networks involved in phrenic nerve stimulation preventing mechanical ventilation induced diaphragm dysfunctionScientific ReportsDecember 15, 2025Mingming Zhang, Fangyuan Li, Min Wang, Lei Li, Wenyan Hao, Xuejiao Duan, Dong Zhangprepared from 1 μg of total RNA per sample using the NEBNext Small RNA Library Prep Kit for Illumina (NEB #E7330S, USA). The procedure involved sequential 3′ and 5′ adapter ligation, reverse transcription to cDNA,

(14) A cholesterol-responsive hepatic tRNA-derived small RNA regulates cholesterol homeostasis and atherosclerosis developmentNat CommunDecember 15, 2025Xiuchun Li, Rebecca Hernandez, Xudong Zhang, Sijie Tang, Xiaohong Yuan, Jing Wu, Kathy Pham, Hukam C. Rawal, Erica C. Heinrich, Shenglong Zhang, et al.(University of California, Riverside, CA) as previously described22,37. The adapters (New England Biolabs, E7330S) were ligated sequentially (3’ adapter, revers transcription primer, then 5’ adapter). First-strand cDNA synthesis

(15) Beyond RNA modification: a novel role for tRNA modifying enzyme in oxidative stress response and metabolismNucleic Acids ResDecember 11, 2025Louna Fruchard, Claudia Sudol, Caroline Rouard, Aurore Treffkorn-Maurau, Léo Hardy, Julia Bos, Magalie Duchateau, Quentin Giai Gianetto, Mariette Matondo, Frédéric Bonhomme, et al.fragments were converted to a library using the NEBNext Small RNA Library Prep Set for Illumina® (NEB ref E7330S, USA) following the manufacturer’s recommendations. The DNA library was quantified using a fluorometer

(16) Technical considerations and review of urinary microRNAs as biomarkers for chronic kidney disease in dogs and catsVeterinary clinical pathologyDecember 1, 2025Chu CP, Nabity MB.(cat. RS‐200‐0012; Illumina, CA, USA) and the NEBNext Small RNA Library Prep Set for Illumina (cat. E7330S; New England Biolabs, MA, USA). TruSeq requires 1 μg of total RNA in 5 μL of nuclease‐free water (200

(17) Probing the function of Streptomyces albidoflavus J1074 gene XNR_5296 for SPOUT family ribose methyltransferaseMicrobiology SpectrumNovember 26, 2025Vasylyna-Marta Tseduliak, Oksana Koshla, Sophie N. Mulartschyk, Virginie Marchand, Yuri Motorin, Mark Helm, Bohdan Ostashfragments were converted to a library using the NEBNext Small RNA Library Prep Set for Illumina (NEB ref E7330S, USA) following the manufacturer’s recommendations. DNA library was quantified using a fluorometer (Qubit 2.0 fluorometer

(18) tRNA Modification Landscapes in Streptococci: Shared Losses and Clade-Specific AdaptationsbioRxivNovember 11, 2025Ho-Ching Tiffany Tsui, Chi-Kong Chan, Yifeng Yuan, Roba Elias, Jingjing Sun, Virginie Marchand, Marshall Jaroch, Guangxin Sun, Irfan Manzoor, Ana Kutchuashvili, et al.converted to sequencing library using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB ref E7330S, USA) following the manufacturer’s recommendations. DNA library was quantified using a fluorometer (Qubit 3.0 fluorometer

(19) ALKBH1 drives tumorigenesis and drug resistance via tRNA decoding reprogramming and codon-biased translationCancer DiscovNovember 3, 2025Chao Shen, Yuan Che, Keren Zhou, Kitty Wang, Wei Li, Diyuan Xue, Tong Wu, Lu Yang, Meiling Chen, Yue Sheng, et al.Research), and libraries were prepared using the NEBNext small RNA library prep kit (New England BioLabs, E7330S). Libraries were sequenced on an Illumina Hiseq2500 platform. Proteomics Sample preparation was performed

(20) ALKBH1 Drives Tumorigenesis and Drug Resistance via tRNA-decoding Reprogramming and Codon-biased TranslationCancer DiscoveryNovember 3, 2025Chao Shen, Yuan Che, Keren Zhou, Kitty Wang, Wei Li, Diyuan Xue, Tong Wu, Lu Yang, Meiling Chen, Yue Sheng, et al.Research), and libraries were prepared using the NEBNext small RNA library prep kit (New England BioLabs, E7330S). Libraries were sequenced on an Illumina Hiseq2500 platform. Proteomics Sample preparation was performed

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NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible)

NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) from New England Biolabs

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NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) from New England Biolabs

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