NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) E7330S from New England Biolabs

Product Specs
ItemNEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible)
CompanyNew England Biolabs
Price $1,236.00 Inquire
Catalog NumberE7330S
Quantity24 reactions
ApplicationsDNA Analysis, Library Prep, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A trans -piRNA network and transcriptional antagonism shape piRNA cluster functionbioRxivApril 9, 2026Yicheng Luo, Daniel Siqueira Siqueira de Oliveira, Peng He, Alexei A. Aravinand purified. Small RNA libraries were generated using the NEBNext Small RNA Library Prep Kit (NEB #E7330S) and sequenced on an Illumina HiSeq 2500 platform. RT-qPCR Approximately 20 ovaries were dissected

(2) The bladder cancer m6A landscape is defined by global methylation dilution and focal 3′-UTR hypermethylationEMBO ReportsMarch 23, 2026Jonas Koch, Jinyun Xu, Felix Bormann, Vitor Coutinho Carneiro, Manuel Neuberger, Katja Nitschke, Malin Nientiedt, Philipp Erben, Maurice Stephan Michel, Manuel Rodriguez-Paredes, et al.,New England Biolabs,Cat M0201S NEBNext Small RNA Library Prep Set for Illumina,New England Biolabs,Cat E7330S NEBNext Multiplex Oligos for Illumina (Index Primer Sets 1 and 3),New England Biolabs,Cat E7335S and

(3) Joint transcriptome-wide and proteomic analysis of the mechanisms underlying the regulation of sheep wool fiber diameterAnimal BioscienceFebruary 1, 2026Lin Yue, Zengkui Lu, Tingting Guo, Bowen Chen, Jianbin Liu, Chao Yuanconstructed, purified, and sequenced using the NEBNext Small RNA Library Prep Set for Illumina kit (NEB#E7330S; NEB). Filtering of low quality reads, The length distribution of the clean sequences in the reference

(4) Conserved shifts in sperm small non-coding RNA profiles during mouse and human agingThe EMBO JournalJanuary 20, 2026Junchao Shi, Xudong Zhang, Chen Cai, Shichao Liu, Jiancheng Yu, Emma R James, Lihua Liu, Benjamin R Emery, Megan R McMurray Bires, Elizabeth Torres-Arce, et al.sourced from the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs, Catalog No. E7330S) and were ligated in sequence. First, a 3′ adapter was added under the following reaction conditions:

(5) Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domainsMolecular CellJanuary 8, 2026Lin Guo, Jacob R. Mann, Jocelyn C. Mauna, Katie E. Copley, Hejia Wang, Jack D. Rubien, Cristian A. Bergmann, Jenny L. Carey, Jessica Merjane, Marilyn Ngo, et al.QuikChange Site-Directed Mutagenesis Kit,Agilent,Cat# 210518 NEBNext® Small RNA Library Prep Set for Illumina®,New England Biolabs,Cat# E7330S Deposited data,, RNA sequencing data,Gene Expression Omnibus,GSE249059.Token:irkdymqknvihfkv "Gel,

(6) Paternal Microplastic Exposure Alters Sperm Small Noncoding RNAs and Affects Offspring Metabolic Health in MiceJournal of the Endocrine SocietyJanuary 7, 2026Seung Hyun Park, Jianfei Pan, Xudong Zhang, Ting-An Lin, Sijie Tang, Xiuchun Li, Sihem Cheloufi, Qi Chen, Tong Zhou, Changcheng ZhouRNase inhibitor). TRIzol Reagent was used again for RNA purification. Adapters (New England Biolabs, E7330S) were ligated to the purified RNA, followed by first-strand cDNA synthesis and library preparation.

(7) Identification of competing endogenous RNA networks involved in phrenic nerve stimulation preventing mechanical ventilation induced diaphragm dysfunctionScientific ReportsDecember 15, 2025Mingming Zhang, Fangyuan Li, Min Wang, Lei Li, Wenyan Hao, Xuejiao Duan, Dong Zhangprepared from 1 μg of total RNA per sample using the NEBNext Small RNA Library Prep Kit for Illumina (NEB #E7330S, USA). The procedure involved sequential 3′ and 5′ adapter ligation, reverse transcription to cDNA,

(8) A cholesterol-responsive hepatic tRNA-derived small RNA regulates cholesterol homeostasis and atherosclerosis developmentNat CommunDecember 15, 2025Xiuchun Li, Rebecca Hernandez, Xudong Zhang, Sijie Tang, Xiaohong Yuan, Jing Wu, Kathy Pham, Hukam C. Rawal, Erica C. Heinrich, Shenglong Zhang, et al.(University of California, Riverside, CA) as previously described22,37. The adapters (New England Biolabs, E7330S) were ligated sequentially (3’ adapter, revers transcription primer, then 5’ adapter). First-strand cDNA synthesis

(9) Beyond RNA modification: a novel role for tRNA modifying enzyme in oxidative stress response and metabolismNucleic Acids ResDecember 11, 2025Louna Fruchard, Claudia Sudol, Caroline Rouard, Aurore Treffkorn-Maurau, Léo Hardy, Julia Bos, Magalie Duchateau, Quentin Giai Gianetto, Mariette Matondo, Frédéric Bonhomme, et al.fragments were converted to a library using the NEBNext Small RNA Library Prep Set for Illumina® (NEB ref E7330S, USA) following the manufacturer’s recommendations. The DNA library was quantified using a fluorometer

(10) Technical considerations and review of urinary microRNAs as biomarkers for chronic kidney disease in dogs and catsVeterinary Clinical PathologyDecember 1, 2025Candice P. Chu, Mary B. Nabity(cat. RS‐200‐0012; Illumina, CA, USA) and the NEBNext Small RNA Library Prep Set for Illumina (cat. E7330S; New England Biolabs, MA, USA). TruSeq requires 1 μg of total RNA in 5 μL of nuclease‐free water (200

(11) Probing the function of Streptomyces albidoflavus J1074 gene XNR_5296 for SPOUT family ribose methyltransferaseMicrobiology SpectrumNovember 26, 2025Vasylyna-Marta Tseduliak, Oksana Koshla, Sophie N. Mulartschyk, Virginie Marchand, Yuri Motorin, Mark Helm, Bohdan Ostashfragments were converted to a library using the NEBNext Small RNA Library Prep Set for Illumina (NEB ref E7330S, USA) following the manufacturer’s recommendations. DNA library was quantified using a fluorometer (Qubit 2.0 fluorometer

(12) tRNA Modification Landscapes in Streptococci: Shared Losses and Clade-Specific AdaptationsbioRxivNovember 11, 2025Ho-Ching Tiffany Tsui, Chi-Kong Chan, Yifeng Yuan, Roba Elias, Jingjing Sun, Virginie Marchand, Marshall Jaroch, Guangxin Sun, Irfan Manzoor, Ana Kutchuashvili, et al.converted to sequencing library using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB ref E7330S, USA) following the manufacturer’s recommendations. DNA library was quantified using a fluorometer (Qubit 3.0 fluorometer

(13) ALKBH1 Drives Tumorigenesis and Drug Resistance via tRNA-decoding Reprogramming and Codon-biased TranslationCancer DiscoveryNovember 3, 2025Chao Shen, Yuan Che, Keren Zhou, Kitty Wang, Wei Li, Diyuan Xue, Tong Wu, Lu Yang, Meiling Chen, Yue Sheng, et al.Research), and libraries were prepared using the NEBNext small RNA library prep kit (New England BioLabs, E7330S). Libraries were sequenced on an Illumina Hiseq2500 platform. Proteomics Sample preparation was performed

(14) Nuclear 2'-O-methylation regulates RNA splicing through its binding protein FUBP1Sci AdvOctober 17, 2025Boyang Gao, Bochen Jiang, Zhongyu Zou, Bei Liu, Weijin Liu, Li Chen, Lisheng Zhang, Chuan HeEO0491), with purified RNA used to construct libraries with NEBNext Small RNA Library Prep Set (NEB, E7330S). Sequencing was performed by Illumina NovaSeq 6000 paired-end 50-bp reads. Bioinformatic analysis

(15) ALKBH8-mediated codon-specific translation promotes colorectal tumorigenesisNat CommunOctober 13, 2025Yu Qian, Canlan Wu, Saisai Wei, Sujun Yan, Junxuan Peng, Lei Yu, Yunyi Gao, Jingyu Hou, Wentao Yu, Zhanghui Chen, et al.B0201S, NEB). Sequencing libraries were generated with the Small RNA Library Prep Set for Illumina (E7330S, NEB) following the manufacturer’s instructions. Single-cell RNA sequencing of mouse colorectal tumors Colorectal tumors

(16) Tumor Intrinsic METTL5 Modulates ATF4 Translation to Prevent T Cell‐Induced Ferroptosis in Ovarian CancerAdvanced ScienceOctober 3, 2025Jiakai Hou, Cheng‐Wei Ju, Nicholas A. Egan, Yanjun Wei, Yunfei Wang, Minghao Dang, Tianyi Zhou, Leilei Shi, Ningbo Zheng, Si Chen, et al.samples were used to prepare libraries for sequencing by using the NEBNext Small RNA Library Prep Kit (#E7330S, New England Biolabs) according to the manufacturer's instructions. An Illumina NovaSeqX at The University

(17) Blood flow-restricted resistance training modulates miRNAs to improve early hypertensive cardiac functionPLOS OneSeptember 25, 2025Zhaowen Tan, Hao Zhu, Yan Zhao, Yuchan Zheng, Jie Zhang[8]. 2.6. MiRNA sequencing The NEBNext Small RNA Library Prep Set for Illumina Kit (Cat. No. NEB#E7330S, NEB, USA) was used to construct miRNA libraries: 1 µg of total RNA was amplified via junction ligation,

(18) Bone remodeling stimulated by Wnt-mediated mitophagy regulated extracellular vesicles in subchondral bone contributes to osteoarthritis developmentTheranosticsSeptember 21, 2025Yuyuan Gu, Qirong Zhou, Shihao Sheng, Huijian Yang, Dan Huang, Qin Zhang, Hao Zhang, Zijian Cao, Yuanwei Zhang, Zuhao Li, et al.(Ambion). Small RNA libraries were then constructed using the Small RNA Library Prep Set for Illumina (NEB#E7330S) following the supplier's protocol, and quality was verified on the Agilent 2100 Bioanalyzer. Differential

(19) Specificity and mechanism of tRNA cleavage by the AriB Toprim nuclease of the PARIS bacterial immune systemPhilosophical Transactions of the Royal Society B: Biological SciencesSeptember 4, 2025Svetlana Belukhina, Baptiste Saudemont, Florence Depardieu, Tom Lorthios, Tinashe P. Maviza, Alexei Livenskyi, Marina Serebryakova, Maria Aleksandrova, Elizaveta Ukholkina, Nadezhda Burmistrova, et al.preparation was performed using the commercial kit NEBNext® Small RNA Library Prep Set for Illumina (no. E7330S, NEB, USA) following the manufacturer’s instructions. DNA libraries were quantified using a Qubit 2.0 fluorometer

(20) Copy number determination of sperm-borne small RNAs implied in the intergenerational inheritance of metabolic syndromesRNAAugust 1, 2025Lisa Koenig, Victoria Guggenberger, Kristeli Eleftheriou, Zsuzsanna Pinter, Alessandro Marotto, Christoph R. Kreutz, Mark Wossidlo, Virginie Marchand, Yuri Motorin, Matthias R. Schaeferwere directly converted to a library using the NEBNext Small RNA Library Prep Set for Illumina (NEB E7330S, USA), following the manufacturer's recommendations. MSC04–06, corresponding to small RNA pools 1, 2,

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NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible)

NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) from New England Biolabs

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NEBNext Small RNA Library Prep Set for Illumina (Multiplex Compatible) from New England Biolabs

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