PURExpress(aa, tRNA) Kit E6840S from New England Biolabs

Product Specs
ItemPURExpress(aa, tRNA) Kit
CompanyNew England Biolabs
Price $389.00 Inquire
Catalog NumberE6840S
Quantity10 reactions
ApplicationsProteomics & Glycomics
Temperature-80 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multiple Adenylate-Forming Enzymes Contribute to Biosynthesis of the DPO Quorum-Sensing AutoinducerACS Chemical BiologyFebruary 20, 2026Delaney M. Lacey, Gabriel D. D’Agostino, Emilee E. Shine, Bonnie L. Basslerfreeze-thaw cycles and held at -80 °C. tRNAAll was obtained from the PURExpress (Δ (aa, tRNA) Kit #E6840S, NEB). Reactions were carried out as above without pyrophosphatase and with 1 μM of tRNAAll, tRNAAla,

(2) Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free systemNat CommunJuly 5, 2025Fanjun Li, Amogh Kumar Baranwal, Sebastian J. Maerkladded the sfGFP template (4 nM) and various concentrations of uniform IVT tRNAs or E. coli tRNAs (NEB, E6840S) to the ΔtRNA PURE system and incubated reactions at 30 °C for 16 h while measuring fluorescence produced

(3) Membraneless protocell confined by a heat flowNature PhysicsJune 26, 2025Alexander Floroni, Noël Yeh Martín, Thomas Matreux, Laura I. Weise, Sheref S. Mansy, Hannes Mutschler, Christof B. Mast, Dieter Braunallow the inflow and outflow of water or feeding solution (amino acids and tRNAs provided in the kit; E6840S, NEB) at the top of the thermophoretic chambers through a 1-mm-diameter open channel connecting the

(4) Expanded ribosomal synthesis of non-standard cyclic backbones in vitroNat CommunMay 28, 2025Kanghun Lee, Hyeongwoo Park, Ravi Kumar Devarapalli, Dahye Im, Jongcheol Seo, Joongoo Leefrom being aminoacylated and participating in translation. The PURExpressTM Δ (aa, tRNA) kit (NEB, E6840S) was used for in vitro ribosomal synthesis and the reaction mixtures were incubated at 37 °C for 2 h.

(5) Inducible auto-phosphorylation regulates a widespread family of nucleotidyltransferase toxinsNature CommunicationsSeptember 4, 2024Tom J. Arrowsmith, Xibing Xu, Shangze Xu, Ben Usher, Peter Stokes, Megan Guest, Agnieszka K. Bronowska, Pierre Genevaux, Tim R. Blowerabsence or presence of the toxin. To pre-incubate MenT1 with tRNA, a PURExpress (Δaa, tRNA) kit (NEB, E6840S) was used. 0.33 µl tRNA from the kit was incubated with 0, 2.5, 5, and 10 µM MenT1 or MenT1-p for 3

(6) Cheminformatics-Guided Cell-Free Exploration of Peptide Natural ProductsJournal of the American Chemical SocietyMarch 27, 2024Jarrett M. Pelton, Joshua E. Hochuli, Patric W. Sadecki, Takayuki Katoh, Hiroaki Suga, Leslie M. Hicks, Eugene N. Muratov, Alexander Tropsha, Albert A. Bowerscyclization. Lastly, translations were carried out with either a standard (NEB PURExpress product #E6840S) or a customized PURE kit (PUREfrex systems product #PFC-Z9901), which allowed for optimized concentrations

(7) Contribution of tRNA sequence and modifications to the decoding preferences of E. coli and M. mycoides tRNAGlyUCC for synonymous glycine codonsNucleic Acids ResearchFebruary 9, 2024Maria Kompatscher, Karolina Bartosik, Kevin Erharter, Raphael Plangger, Fabian Sebastian Juen, Christoph Kreutz, Ronald Micura, Eric Westhof, Matthias D Erlacherdepletion of E. coli tRNA bulk Native E. coli tRNA bulk included in the PURExpress® Δ(aa, tRNA) Kit (NEB, E6840S) was depleted for up to three tRNAGly isoacceptors by RNase H. The protocol for the selective depletion

(8) MenT nucleotidyltransferase toxins extend tRNA acceptor stems and can be inhibited by asymmetrical antitoxin bindingNat CommunAugust 17, 2023Xibing Xu, Ben Usher, Claude Gutierrez, Roland Barriot, Tom J. Arrowsmith, Xue Han, Peter Redder, Olivier Neyrolles, Tim R. Blower, Pierre Genevauxby Image Lab software (Bio-Rad). To pre-incubate MenT1 with tRNA, a PURExpress Δ(aa, tRNA) kit (NEB, E6840S) was used. 0.33 µl tRNA from the kit was incubated with 0, 2.5, 5, 10 µM MenT1 for 3 h at 37 °C, then

(9) Expanding the substrate scope of pyrrolysyl-transfer RNA synthetase enzymes to include non-α-amino acids in vitro and in vivoNature ChemistryJuly 1, 2023Riley Fricke, Cameron V. Swenson, Leah Tang Roe, Noah Xue Hamlish, Bhavana Shah, Zhongqi Zhang, Elise Ficaretta, Omer Ad, Sarah Smaga, Christine L. Gee, et al.X-Val-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (XV-Flag, where X = 7, 13, 14 or 15) was carried out using the PURExpress Δ (aa, tRNA) Kit (NEB, E6840S) according to a previous protocol with slight modifications17. The XV-Flag peptides were produced from

(10) Suppression of Formylation Provides an Alternative Approach to Vacant Codon Creation in Bacterial In Vitro TranslationAngewandte Chemie International EditionDecember 1, 2020Minglong Liu, Vito Thijssen, Seino A. K. JongkeesInformation Chemicals were purchased from Sigma Aldrich, Alfa Aesar and used without further purification. PURExpress (E6840S) was purchased from New England Biolabs. UPLC-MS experiments were performed on an Agilent 6560 Ion Mobility

(11) Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitroNature CommunicationsDecember 1, 2020Joongoo Lee, Kevin J. Schwarz, Do Soon Kim, Jeffrey S. Moore, Michael C. Jewettendogenous tRNAs from being aminoacylated and used in translation. The PURExpressTM Δ (aa, tRNA) kit (NEB, E6840S) was used for polyamide synthesis reaction and the reaction mixtures were incubated at 37 °C for 3 h.

(12) Defects in the Assembly of Ribosomes Selected for β-Amino Acid IncorporationBiochemistryNovember 12, 2019Fred R. Ward, Zoe L. Watson, Omer Ad, Alanna Schepartz, Jamie H. D. CateIn vitro transcription/translation of (fMVF-Flag was carried out using the combination of PureExpress (ΔtRNA, Δaa (E6840S)) and PureExpress (Δribosome (E3313S)) kits (NEB) with the following modifications. To generate the

(13) Fail-safe genetic codes designed to intrinsically contain engineered organismsNucleic Acids ResearchNovember 4, 2019Jonathan Calles, Isaac Justice, Detravious Brinkley, Alexa Garcia, Drew Endywas prepared by supplementing a PURExpress in vitro expression system lacking tRNAs (PURE ΔtRNA, NEB# E6840S). A standard-encoded expression system was built by supplementing PURE ΔtRNA with control tRNAs supplied

(14) Directed Evolution of Glycopeptides Using mRNA DisplayMethods in EnzymologySeptember 23, 2017Satoru Horiya, Jennifer K. Bailey, Isaac J. Kraussincluding commercial BL21 ribosomes, order PURExpress Δ(amino acids, tRNA) as NEB standard catalog item E6840S, and modify this recipe accordingly. Using in-house PURE system: prepare components described in the

(15) Mechanism of orphan subunit recognition during assembly quality controlCellJanuary 1, 0001"Yagita Y, Zavodszky E, Peak-Chew SY, Hegde RS."Synthesis Kit,New England Biolabs,Cat#E6800S "PURExpress Δ (aa, tRNA) Kit",New England Biolabs,Cat#E6840S ,, Deposited data,Deposited data,Deposited data ,, Proteomics data,This paper,PRIDE: PXD042773 "Cryo-EM

Be the First to Write a Review!

PURExpress(aa, tRNA) Kit

PURExpress(aa, tRNA) Kit from New England Biolabs

New England Biolabs

Citations (15)

Reviews

PURExpress(aa, tRNA) Kit from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map