Zombie Aqua™ Fixable Viability Kit 423102 from BioLegend

Product Specs
ItemZombie Aqua™ Fixable Viability Kit
CompanyBioLegend
Price $335.00Supplier Page View Company Product Page
Catalog Number423102
ApplicationsFC - Quality tested, ICFC, ICC - Verified
Quantity500 tests

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) DNA prime and peptide boost immunization elicits robust neoantigen-specific CD8 + T cell responses and therapeutic protection in mouse tumor modelsOncoImmunologyDecember 31, 2026Pablo Morgado-Cáceres, Francisca Hofmann-Vega, Diego Figueroa, Juan Saavedra-Almarza, Felipe Gálvez-Cancino, Ximena Díaz, Evelyn Menares, Eduardo Roa, Sofia Hidalgo, Manuel Varas-Godoy, et al.in PBS-FBS 2% by 10 min at 4 °C, washed twice with PBS, incubated with Zombie AquaTM (Biolegend, ref 423102) viability dye diluted in PBS by 20 min at 4 °C, washed twice with PBS, incubated with the antibodies

(2) A commensal protozoan exacerbates acetaminophen-induced liver injury by producing free sphingosineGut MicrobesDecember 31, 2026Xiaoqing Yan, Yusi Shen, Qian Sun, Xiuwen Zhu, Qiuchong Chen, Longxiang Liao, Yuan Zhou, Wanpeng Cheng, Zhen Shi, Zhuanzhuan Liu, et al.with APAP plus sphingosine or TMAO for 24 h, and stained using the Zombie Aqua™ Fixable Viability Kit (423101, Biolegend). For western blot assay, cells were treated with APAP plus sphingosine or TMAO for 12 h,

(3) Fusobacterium nucleatum-derived succinic acid aggravates colitis by triggering macrophage pro-inflammatory phenotypic transformation via SUCNR1/NF-κB axisGut MicrobesDecember 31, 2026Suqi Zeng, Shanshan Jiang, Jianxuan Sun, Yan Chen, Meiqi Qiu, Jiao Li, Qingzhi Lan, Yu Pu, Zongbiao Tan, Xingzhou Guo, et al.were stained according to the respective antibody manufacturers’ protocols. Zombie Aqua viability dye (423102, BioLegend, USA) was used to discriminate live and dead cells. For BMDM, the same staining protocol

(4) A NANOBODY molecule that blocks MerTK ectodomain cleavage in vitro and in vivoMAbsDecember 31, 2026Linde Duprez, Ayse Kilic, Hélène Bruwiere, Karim Fekir, Dennis Vlaeminck, Camille De Craene, Daan Geiregat, Kirsten Paesen, Lien Leutenez, Stijn Lambrecht, et al.30 µM HSA. Cells were washed and stained with Zombie Aqua Viability Dye (Biolegend, cat. no. 423101/423102), human Fc-block and mouse anti-FLAG (Sigma-Aldrich, cat. no. F1804) or ABH0074 and anti-mouse IgG APC

(5) Bone targeted microenvironment actuated engineered exosomes for precision therapy of IBD associated bone lossBioactive MaterialsOctober 1, 2026Jiang Liu, Cong Wang, Taojin Feng, Siyu Chen, Siliang Ge, Yue Zhao, Ming Chen, Zhongqi Wang, Weier Bao, Pincong Fu, et al.in the presence of brefeldin A. The cells were then stained with Zombie Aqua Fixable Viability Kit (423102, BioLegend) to exclude dead cells and with FITC anti-mouse CD4 (100406, BioLegend) antibody for surface

(6) RNF180 loss stabilizes BCAS4 to enhance PD-L1 expression and immune evasion in hepatocellular carcinomaiScienceSeptember 18, 2026Ke Ye, Xiupeng Cai, Xuefan Dongthen FSC-H/FSC-A to exclude doublets. Live cells were identified as Zombie Aqua-negative (BioLegend, #423101), followed by gating on human CD45+ leukocytes and human CD3+ T cells. CD8A identified the total CD8-lineage

(7) CAR design equips diverse immune cells with unique antigen-specific activation identitiesMolecular Therapy OncologySeptember 17, 2026Ayano Sugiyama-Finnis, Takaharu Kimura, Kiyoko Izawa, Yuko Kawano, Takao Yogo, Hyojung Jeon, Tsuyoshi Fukushima, Ryoji Ito, Satoshi YamazakiA549-CD19+mKate+ cells, staining with Zombie Aqua Fixable Viability Kit (BioLegend, San Diego, California, # 423101) at a 1:500 dilution for 20 min at 4°C was conducted. Following washing in PBS supplemented with 2%

(8) Space radiation promotes clonal hematopoiesis and hematologic disease upon aging in a driver gene and sex-specific manneriScienceAugust 21, 2026Megan A. Evans, Heather Doviak, Ariel H. Polizio, Jesse D. Cochran, Leslie R. Kim, Eunbee Park, Mark C. Thel, Christopher R. Xie, Ajay R. Srinivasan, Lucila Marino, et al.Amersham ECL Prime Western Blotting Detection Reagent,Cytiva,Cat No. RPN2232 Streptavidin BV650 [bone marrow 2],BioLegend,Cat No. 423102 Zombie Aqua Fixable Viability Stain,BioLegend,Cat No. 423101 eBioscience 1 × RBC lysis buffer,"Invitrogen,

(9) Preclinical Development of ARV-2001, an Intradermally Administered mRNA-Lipid Nanoparticle Immunotherapeutic for the Treatment of HPV-16-Positive Cervical High-Grade Squamous Intraepithelial LesionsVaccines (Basel)August 19, 2026Zhengxiang He, Huabin Zhu, Ju Hyeong Jeon, Jianzhu Chen, Gregory M. Glenn, Renhuan Xuintracellular staining. In brief, cells were stained with Zombie Aqua Fixable viability dye (BioLegend, Cat: 423102) in PBS for 20 min at room temperature (RT). Cells were then fixed and permeabilized with Foxp3/Transcription

(10) Tumor-distal mediastinal lymph nodes harbor tumor-related progenitor exhausted T cells in lung cancer after neoadjuvant chemoimmunotherapyCell Rep MedAugust 18, 2026Bin Zhang, Pengpeng Zhang, Zhanshuo Zhang, Jiping Xie, Xiaonan Cui, Chen Chen, Qiang Zhang, Yuechen Cui, Leina Sun, Shuo Wang, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, Zombie viability dye,BioLegend,Cat# 423102 PMABD,Biosciences,Cat# 550583 Permeabilization buffer,eBioscience,Cat# 00-5523-00 Collagenase IV,Biosharp,Cat#

(11) Glucagon-like peptide-1 receptor agonist prevents pulmonary fibrosis following acute COVID-19 infection associated with type 2 diabetesJournal of VirologyAugust 18, 2026Runhong Zhou, Ming Yue, Qing Shen, Na Liu, Yuting Chen, Pui Wang, Kyungmin Kim, Ranyao Yang, Honglin Chen, Kwok-Yung Yuen, et al.APC-Fire750 anti-CD45. All cells were also stained with dead cell dye (Zombie Aqua, Biolegend Cat# 423102). Stained cells were acquired by FACSAriaIII Flow Cytometer (BD Biosciences) and analyzed with FlowJo

(12) Nilotinib induces immunogenic cuproptosis to potentiate cancer immunotherapyCell Reports MedicineAugust 18, 2026Lei Yao, Deze Zhao, Yu Meng, Yihuang Liu, Hui Su, Danyao Chen, Ziyu Guo, Daishi Li, Linfeng Li, Qian Zhou, et al.Cat# 101320). Cell viability was assessed with the Zombie Aqua Fixable Viability Kit (BioLegend, Cat# 423102). For intracellular cytokine staining, cells were stimulated in vitro for 5 h with Cell Stimulation

(13) Evolution of SPP1+ cavity macrophage-mediated immunotherapy resistance in peritoneal metastasis of colorectal cancerCell Reports MedicineAugust 18, 2026Xiaomeng Dai, Chunyu Lai, Libing Hong, Yuzhi Jin, Jinlin Cheng, Haimeng Yan, Xuqi Sun, Bin Li, Chuan Liu, Hangyu Zhang, et al.#156604), viability dyes [Zombie NIR APC-Cy7 (BioLegend #423105), and Zombie NIR BV510 (BioLegend #423101)], and the following fluorescently conjugated antibodies: APCCD206(BioLegend #141708), PE-arginase 1

(14) CCL5 exposure promotes acute and transient megakaryopoiesis and hematopoietic progenitor expansionStem Cell ReportsAugust 11, 2026Maria N. Barrachina, Dong H. Lee, Virginia Camacho, Karen G. Wang, Ethan Walsey, Estelle Carminita, Isabelle C. Becker, Siobhan Branfield, Daniela Freire, Emma Nikols, et al.BioLegend), and anti-CD19 (115510, BioLegend) as described. Live cells were distinguished using Zombie Aqua (423102, BioLegend). HSPC populations were identified by spectral flow cytometry (Cytek Aurora) as previously

(15) Pseudomonas aeruginosa infection causes lysosomal dysfunction in the cystic fibrosis bronchial epitheliumInfection and ImmunityAugust 11, 2026Emma Lea Matthews, Meghan June Hirsch, Kuen-You Tsai, Hannah J. McIntire-Ray, Viviana Acevedo Rua, Angela N. Morales, Luke I. Jones, Jarrod W. Barnes, Megan R. Kiedrowski, Stefanie KrickCells were resuspended and then incubated with 1:10 diluted live-dead stain Zombie Aqua (BioLegend 423102) in PBS + EDTA on ice for 10 min in the dark. Cells were spun at 800 × g for 10 min at 4°C, washed with

(16) PARP Inhibitors plus Anlotinib as Bridging Therapy for Armored CAR-T Cells in Ovarian Cancer Enhances Infiltration and Antitumor EfficacyAdv Sci (Weinh)August 10, 2026Huayi Li, Minghua Xiang, Qiuyang Xu, Jiahao Liu, Xiaofei Jiao, Wei Mu, Xiaoying Lv, Kangjia Tao, Yu Xu, Dongchen Zhou, et al.resulting single‐cell suspensions were washed with PBS, stained with a Zombie Aqua Fixable Viability Kit (#423102, BioLegend, San Diego, CA, USA) to exclude non‐viable cells, and incubated with mouse or human TruStain FcX

(17) Subclinical cholestasis is a hallmark of gut dysbiosis causing resistance to cancer immunotherapyCancer CellAugust 10, 2026Anne-Laure Mallard de La Varende, Ai-Ling Tian, Simon Thomas, Imran Lahmar, Meriem Messaoudene, Sijing Li, Omar Motiño, Hortense Guillaume-dit-Taunière, Valerio Iebba, Yoan Hurtado, et al.proteins","Chemicals, peptides, and recombinant proteins" ,, Zombie Aqua™ Fixable Viability Kit,Biolegend,Cat# 423102 LIVE/DEAD™ Fixable Aqua dye,Thermo Fisher Scientific,Cat# L34966 "CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry

(18) Microbial and metabolic signatures of humanized microbiome mice associate with Tlr2 expression and Th17 immune programmingFront ImmunolAugust 7, 2026Alexis N. Cox-Holmes, George B. H. Green, Anna Claire E. Potier, Yong Wang, Dongquan Chen, Casey D. Morrow, Braden C. McFarland155837), CD45-APC (Biolegend, CAT# 103112; 1:200), and Zombie Aqua fixable viability die (Biolegend, CAT# 423101; 1:500) for 30 min at 4˚C. Cells were sorted for live CD45+ single cells on a BD FACS ARIA SORP in the

(19) Targeting the αvβ5 Integrin Modifies the TGF-β-Rich Tumor Microenvironment of Pancreatic CancerCancer ResAugust 4, 2026Kodai Suzuki, Yuki Kunisada, Yukihito Kuroda, Hotaka Kawai, Tatiana Hurtado de Mendoza, Evangeline S. Mose, Shingo Eikawa, Henri Havia, Tero A. H. Järvinen, Caisheng Lu, et al.(clone RMV-7; Cat# 740755, RRID: AB_2870648; BD Biosciences), or Aqua™ Fixable Viability Kit (Cat# 423101; BioLegend). Intracellular staining of immune cells was performed using the following Abs and a Foxp3

(20) Respiratory Klebsiella pneumoniae infection transiently alters neutrophil responsiveness and enhances distal allergic inflammationFront ImmunolAugust 4, 2026Xilan Xiao, Jing Li, Tongqian Wu, Shirong Yan, Yan Wang, Rongtao Yang, Ziqian Guo, Wengui Gong, Zhicheng Hu, Fang Yuto flow cytometric analysis. 1 μl of prepared Zombie Aqua Fixable Viability Kit (BioLegend, Cat. No. 423101, USA) was added and incubated for 15 min at room temperature, 2 μL Fc receptors were blocked with an

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(1)

12/5/2023Viability Kit From BioLegend Requires Titration as It Provides Bright Signal Samantha ChinI have used other Zombie Viability dyes in the past and here have Zombie Aqua to stain for the viability of my tumor cells. Using Zombie Aqua, I was able to determine what percentage of my tumor cells... Read Review
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4/20/2021Decent Fixable Viability Dye for Flow Cytometry Vincent GiacaloneHuman PBMCs were isolated from blood, cultured in various conditions and stained for analysis by flow cytometry
(1)

4/20/2020Blue Zombies Gregor WerbaEasy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.

Zombie Aqua™ Fixable Viability Kit from BioLegend

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Zombie Aqua™ Fixable Viability Kit from BioLegend

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