Zombie Aqua™ Fixable Viability Kit 423102 from BioLegend

Product Specs
ItemZombie Aqua™ Fixable Viability Kit
CompanyBioLegend
Price $335.00Supplier Page View Company Product Page
Catalog Number423102
Quantity500 tests
ApplicationsFC - Quality tested, ICFC, ICC - Verified

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) DNA prime and peptide boost immunization elicits robust neoantigen-specific CD8 + T cell responses and therapeutic protection in mouse tumor modelsOncoImmunologyDecember 31, 2026Pablo Morgado-Cáceres, Francisca Hofmann-Vega, Diego Figueroa, Juan Saavedra-Almarza, Felipe Gálvez-Cancino, Ximena Díaz, Evelyn Menares, Eduardo Roa, Sofia Hidalgo, Manuel Varas-Godoy, et al.in PBS-FBS 2% by 10 min at 4 °C, washed twice with PBS, incubated with Zombie AquaTM (Biolegend, ref 423102) viability dye diluted in PBS by 20 min at 4 °C, washed twice with PBS, incubated with the antibodies

(2) Intratumoral Virus-Like Particles Containing a TLR9 Agonist Combined with Systemic αPD-1 Activate Tumor-Specific CD8+ T CellsCancer Res CommunMay 1, 2026Travis D. Fischer, Caitlin D. Lemke-Miltner, George J. Weinerevaluation. Cells were collected and stained with Zombie Aqua Fixable Viability dye (BioLegend, cat. #423101) or Live/Dead Blue Viability dye (Thermo Fisher Scientific, cat. #L23105) according to the manufacturers’

(3) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,VECTASHIELD Vibrance Antifade Mounting Medium,Vector Laboratories,H-1700-10, "Chemical compound, drug",Zombie Aqua Fixable Viability Kit,BioLegend,423101, "Chemical compound, drug",Zombie NIR Fixable Viability Kit,BioLegend,423105, "Chemical compound,

(4) An In Vitro Model of Macrophage SenescenceCurr ProtocApril 22, 2026Grasiela Torres, Utkarsh Tripathi, Ivan Salladay-Perez, Itzetl Avila, Anthony J. Covarrubias(rat‐monoclonal anti CD11b BV650, BioLegend, cat. no. 101239)Viability dye, e.g., Zombie Aqua (Biolegend, cat no. 423101), optional 1.7‐ml tubesCentrifuge (Sorvall ST40R centrifuge with TX‐1000 rotor, or equivalent)Flow cytometer 1After

(5) Insufficient erythrocyte-derived S1P: A pathogenic driver and diagnostic biolipid for tumor progressioniScienceApril 17, 2026Zhenjiang Li, Weilun Huang, Zhouzhou Yao, Juncheng Wang, Wenhao Xiao, Qi Guo, Changhan Chen, Tingting Xie, Zhiyu Yang, Qingyang Liu, et al.reagents,ECOTOP,Cat# EK-5008 Antifade mounting kit,Beyotime,Cat# P0131 Zombie Aqua™ viability dye,BioLegend,Cat# 423101 ,, Deposited data,Deposited data,Deposited data ,, Raw metabolomics data,This paper,Mendeley Data:

(6) Functional segregation of HIF-1α and AhR controls NK cell responsiveness under hypoxiaiScienceApril 17, 2026Sebastiano Giorgetta, Francesco Cortopassi, Theodoros Chanis, Jing Ni, Margareta P. Correia, Carsten Sticht, Volker Ast, Michael Platten, Adelheid Cerwenka, Ana Stojanovic,MBL International,Cat#B002-5 Torin2,Selleckhem,Cat#S2817 Zombie Aqua™ Fixable Viability Dye,Biolegend,Cat#423102 Cytofix/Cytoperm™ buffer,BD Biosciences,Cat#554714 Phosflow Perm buffer III™,BD Biosciencs,Cat#558050 L-Kynurenine,Selleckhem,Cat#S5839 LEAF™

(7) The Effects of Past COVID-19 and Vaccination on Antibody Levels, Cellular Immunity, and Cytokine Production by Peripheral Blood Mononuclear CellsBiomedicinesApril 17, 2026Yulia A. Desheva, Tatiana V. Gupalova, Polina A. Kudar, Galina F. Leontieva, Igor V. Kudryavtsev, Andrey S. Trulioff, Danila S. Guzenkov, Victoria A. Matyushenko, Elena A. Bormotova, Daniil D. Sokolovsky, et al.750 anti-human CD3 Antibody (cat. 344840, clone SK7), and Zombie Aqua™ Fixable Viability Kit (cat. 423102)) at 4 °C for 20 min in the dark for detection live/dead cells. All antibodies were manufactured by

(8) Enhanced Th1 Cellular Immunity Induced by an RSV-F mRNA Vaccine Rationally Designed Using NLP AlgorithmsVaccines (Basel)April 16, 2026Zhi-Wu Xia, Qi Tang, Jun-Jie Pan, Jing Liu, Lan-Xin Jia, Guo-Mei Zhang, Man-Ni Xie, Jia-Hao Zheng, Chuan-Shuo Lv, Lei Zhang, et al.final 5 h. First, the cells were stained with a Zombie Aqua Fixable Viability Kit (Biolegend, Cat. #423102, 10 min, RT, BioLegend, Inc., San Diego, CA, USA), followed by APC/Cyanine7 anti-mouse CD3 (Biolegend,

(9) Tet2-driven clonal hematopoiesis drives aortic aneurysm via macrophage-to-osteoclast-like differentiationJournal of Clinical InvestigationApril 15, 2026Jun Yonekawa, Yoshimitsu Yura, Junmiao Luo, Katsuhiro Kato, Shuta Ikeda, Yohei Kawai, Tomoki Hattori, Ryotaro Okamoto, Mari Kizuki, Emiri Miura-Yura, et al.incubation with antibodies, dead cells were excluded from the analysis using Zombie Aqua or Violet (catalogs 423102 and 423113, respectively; BioLegend) according to the manufacturer’s instructions. To determine the

(10) Protocol for isolating stromal cells from lymphoid tissue for performing scRNA-seqSTAR ProtocApril 15, 2026Spyridon Makris, Daniel Shewring, Agnesska C. Benjamin, Sophie E. Acton1:100),BioLegend,"Cat# 103127, RRID:AB_493714" Aqua™ Fixable Viability Kit 500 tests,BioLegend,"Cat# 423102, RRID: N/A" CD31 PerCP-Cy5.5 (dilutions in 1:50),BD Biosciences,"Cat# 562861, RRID:AB_2737847" CD31

(11) A novel photosensitizer-based photodynamic therapy reprograms the Kynurenine-AhR axis to boost antitumor immunity in breast cancerRedox BiolApril 15, 2026Yuetong Liu, Ge Hong, Tianjun Liu, Hong LiuAfter RBC lysis, cells were filtered, washed, and stained with the Zombie Aqua™ Fixable Viability Kit (423101, Biolegend) to identify live and dead cells. For blocking non-specific binding, the cells were incubated

(12) Defined culture conditions improve functional properties of mature iPSC-derived macrophages for therapeutic screeningStem Cell Res TherApril 11, 2026Maximilian Schinke, Ingrid Gensch, Tina Rietschel, Ariane Hai Ha Nguyen, Hortense Slevogt, Lothar Jänsch, Nico LachmannPBS; Miltenyi Biotec #130059-901) and Zombie Aqua™ Fixable Viability Kit (1:250 in PBS; Biolegend #423101) for 10 min at room temperature. Thereafter, the cells were stained with fluorescently labelled antibodies

(13) A fully human pan VL9 HLA-E TCRm antibody enables functional dissection of HLA-E biology and checkpoint signalingiScienceApril 9, 2026Soroush Ghaffari, Katherine Upchurch-Ange, Gizem Oter, Trivendra Tripathi, Susanne Gimlin, Debra Wawro Weidanz, Jim Middelburg, Thorbald van Hall, Jon A. Weidanzkit,Roche,Cat#760-052 Ventana Discovery OmniMap kit,Roche,Cat#760-159 Zombie Aqua Fixable Viability Kit,BioLegend,Cat#423102 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, A20-eGFP-Neo/Fluc-Puro,Imanis

(14) Endothelial Elavl1 Is Required for CD8 T-Cell Persistence in AtherosclerosisArterioscler Thromb Vasc BiolApril 9, 2026Sarah-Anne E. Nicholas, Stephen B. Helming, Antoine Ménoret, Christopher L. Pathoulas, Maria M. Xu, Jessica Hensel, Amy L. Kimble, Brent Heineman, Evan R. Jellison, Bo Reese, et al.Fc Block (InVivoMAb anti-mouse CD16/32, BioXcell BE0307), Live/Dead Zombie Aqua (1:100, Biolegend, 423101), CD45.1 (1:200, Thermo, 25-0453-82, clone A20), CD45.2 (1:200, Biolegend, 109823, clone 104), CD3e

(15) Galectin-9high Neutrophils Exacerbate Radiation-Induced FrailtyAging CellApril 7, 2026Zhuo Cheng, Le Ma, Yan Chen, Jialun Li, Yali Dai, Wentao Xie, Yingjie Li, Tianlang Xu, Zhihe Hu, Weilin Huang, et al.blocking reagent (Biolegend #156604) to block and were labeled with Zombie Aqua live/dead dye (Biolegend #423101) for 10 min at room temperature, subsequent staining with fluorescently labeled antibodies. For intracellular

(16) Sex shapes CD64 expression and vaccine-induced monocytic responsesBiol Sex DifferApril 5, 2026Charlotte Sophie Hansen, Julie Sellau, Anastasia Langanz, Leonie Marie Weskamm, Nele Wichern, Annika Bea, Johannes Brandi, Helena Fehling, Nils Groth, Melanie Lütkemeyer, et al.#305036 CX3CR1,PE-Cy7,1:25,2A9-1,BioLegend #341612 HLA-DR,BUV395,1:27,G46-6,BD #564040 Viability,Zombie Aqua,1:1000,,BioLegend #423101 Flow cytometry panel for phenotypic characterization of freshly isolated human PBMCs from whole blood Measured

(17) A stromal PAI1-tPA axis orchestrates immunosuppression in pancreatic cancerSci AdvApril 3, 2026Tenzin Ngodup, Brynn Elson, Ashley M. Mello, Sean Hannifin, Miranda Liu, Yaqing Zhang, Jiaqi Shi, Yatrik M. Shah, Daniel A. Lawrence, Marina Pasca di Magliano, et al.antibodies for 30 min on ice. Dead cells were excluded by staining with a fixable viability stain (no. 423102, BioLegend) for 15 min on ice. Staining for intracellular Foxp3 was performed using mouse Treg cell

(18) Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activitybioRxivApril 3, 2026Oriana Y. Teran Pumar, Elton L VanNoy, Abigail Haffey, Durga Prasad Gannamedi, Christine Isabelle Rafie, Dylan Scott Lykke Lykke Harwood, Julia R. Benedetti, Christine Ann Pittman Pittman Ballard, Erika Ciervo, Pedro Henrique Assenza Tavares Assenza Tavares Coroa, et al.aliquoted for flow staining. After processing, all cells were stained with Zombie Aqua (Biolegend #423101) at 1:500 in PBS for 10 minutes to distinguish live populations and then incubated in FcR mouse blocking

(19) SELL Marks an Effector-Deficient CD8+ T Cell Subset That Promotes Intracerebral Hemorrhage and Responds to Rutin TherapyHum MutatApril 2, 2026Yan Huang, Haochen Xu, Congxia Bai, Jing Liu, Yingying Sun, Jingzhou Chenprotein‐free, Tris‐free PBS. Viability assessment employed Zombie Aqua fixable dye (1:1000 dilution; BioLegend #423101) with 15‐min ambient temperature incubation protected from light. Postwash surface epitope labeling

(20) Non-Glycosylated, Legumain-Cleavable ISACs Drive Potent Anti-Tumor Immunotherapy via a Bystander EffectMolecular Cancer TherapeuticsApril 2, 2026Anqi Zhang, Mohan Reddy Mullapudi, Lina Wu, Michele Yi, Mohammad Asikur Rahman, Aiman A. Yaseen, H. Kayla Zhong, Tracy A. Brooks, L. Nathan TumeyFlow cytometric analysis Cells were stained on ice for 30 minutes with Zombie NIR Fixable Viability Dye (BioLegend, cat. #423101) and a synthesized Cy5-labeled anti-TROP2 antibody. Following staining, samples were washed twice and

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(1)

12/5/2023Viability Kit From BioLegend Requires Titration as It Provides Bright Signal Samantha ChinI have used other Zombie Viability dyes in the past and here have Zombie Aqua to stain for the viability of my tumor cells. Using Zombie Aqua, I was able to determine what percentage of my tumor cells... Read Review
(1)

4/20/2021Decent Fixable Viability Dye for Flow Cytometry Vincent GiacaloneHuman PBMCs were isolated from blood, cultured in various conditions and stained for analysis by flow cytometry
(1)

4/20/2020Blue Zombies Gregor WerbaEasy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.

Zombie Aqua™ Fixable Viability Kit from BioLegend

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Zombie Aqua™ Fixable Viability Kit from BioLegend

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