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Assay Kit
Zombie Aqua(TM) Fixable Viability Kit
Zombie Aqua(TM) Fixable Viability Kit from BioLegend
(5)
Write a review
Citations:
(600)
Supplier Page
Supplier Page from
BioLegend for
Zombie Aqua(TM) Fixable Viability Kit
Description
Zombie Aqua(TM) Fixable Viability Kit
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Zombie Aqua(TM) Fixable Viability Kit
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
423101
Quantity
100 tests
Applications
Flow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC), Immunocytochemistry (ICC)
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BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (600)
(1)
AMPK activation induces immunogenic cell death in AML
Blood Advances
December 26, 2023
Johanna Mondesir, Margherita Ghisi, Laura Poillet, Robert A. Bossong, Oliver Kepp, Guido Kroemer, Jean-Emmanuel Sarry, Jérôme Tamburini, Andrew A. Lane
(
Cell Signaling Technology
, no.
4083S
); Zombie Green (
BioLegend
, no.
423111
); Zombie Aqua (
BioLegend
, no.
423101
); anti-
CALR
antibody, ER marker (Abcam, no. ab 2907); goat anti-rabbit IgG (H+L), secondary antibody
(2)
LFA-1 regulated by IL-2/STAT5 pathway boosts antitumor function of intratumoral CD8+ T cells for improving anti-PD-1 antibody therapy
Oncoimmunology
December 21, 2023
Jiqi Shan, Wei Jing, Yu Ping, Chunyi Shen, Dong Han, Fengsen Liu, Yaqing Liu, Congcong Li, Yi Zhang
cell-surface
staining
, all samples were incubated with a Zombie Aqua Fixable Viability Kit (
BioLegend
, cat. #
423101
) for 15 min at room temperature. To detect cytokine production, cells were treated with a Cell Activation
(3)
Single-cell characterization of human GBM reveals regional differences in tumor-infiltrating leukocyte activation
Elife
December 21, 2023
Philip Schmassmann, Julien Roux, Steffen Dettling, Sabrina Hogan, Tala Shekarian, Tomás A. Martins, Marie-Françoise Ritz, Sylvia Herter, Marina Bacac, Gregor Hutter
and subsequently washed with FACS buffer. To exclude dead cells Zombie Aqua Fixable Viability Kit (#
423102
,
1:100
,
BioLegend
) was added. Acquisition was performed on a CytoFLEX (Beckman). Data was analyzed using
(4)
Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment
Nat Commun
December 20, 2023
Xiaojian Lu, Yanwei Luo, Xichen Nie, Bailing Zhang, Xiaoyan Wang, Ran Li, Guangmin Liu, Qianyin Zhou, Zhizhong Liu, Liqing Fan,
et al.
previously reported using the
flow cytometer
(Cytec, USA)74,77. Zombie Aqua fixable viability kit (catalog:
423101
,
1:200
),
APC-anti-human CD19
(catalog:
302212
,
1:100
), PE-Cy7-anti-human 4-1BB (catalog:
309817
,
1:100
)
(5)
An epitope encoded by uORF of RNF10 elicits a therapeutic anti-tumor immune response
Molecular Therapy - Oncolytics
December 19, 2023
Lili Zeng, Wei Zheng, Jiahui Zhang, Jiawen Wang, Qing Ji, Xinglong Wu, Yaming Meng, Xiaofeng Zhu
were incubated for 5 h at 37°C, stained with Zombie Aqua Fixable Viability Kit (BV525,
BioLegend
, Cat#
423101
), anti-mouse CD8 (PerCP,
BioLegend
, Cat#
100732
), and anti-mouse CD3 (APC-Cy 7, BD
Pharmingen
, Cat#
(6)
Thrombocyte-derived Dickkopf1 promotes macrophage polarization in the Bleomycin-induced lung injury model
Front Immunol
December 15, 2023
Eun-Ah Sung, Min Hee Park, SuJeong Song, Hasan Alanya, Octavian Henegariu, Jinze Liu, E Zeynep Erson-Omay, Patricia J. Sime, Wook-Jin Chae
(
Miltenyi Biotec
). After blocking the Fc Receptor, cells were stained with Zombie Aqua Fixable Viability Kit (
423102
,
BioLegend
). Subsequently, the cells were stained with fluorescent-conjugated antibodies against different
(7)
Hepatitis D infection induces IFN-β-mediated NK cell activation and TRAIL-dependent cytotoxicity
Front Immunol
December 8, 2023
Christopher Groth, Jovana Maric, Irene Garcés Lázaro, Tomáš Hofman, Zhenfeng Zhang, Yi Ni, Franziska Keller, Isabelle Seufert, Maike Hofmann, Christoph Neumann-Haefelin,
et al.
Fixable Viability Kit (
Biolegend
, Cat.:
423105
) Zombie Aqua™ Fixable Viability Kit (
Biolegend
, Cat.:
423101
), fixable viability dye e780 (eBioscience, Cat.: 65-0865) or the BD Horizon™
Fixable Viability Stain 700
(8)
Innate and Adaptive Cell-Mediated Immune Responses to a COVID-19 mRNA Vaccine in Young Children
Open Forum Infect Dis
December 2, 2023
Adriana Weinberg, Michael J Johnson, Krystle Garth, Elena W Y Hsieh, Ross Kedl, Daniela Weiskopf, Mattie Cassaday, Cody Rester, Berenice Cabrera-Martinez, Ryan M Baxter,
et al.
incubation
at 37 °C in a humidified 5% CO2 atmosphere, cells were stained with zombie aqua (
Biolegend
423101
) viability dye and monoclonal antibodies recognizing the following cell surface markers:
CD19
(BV605,
(9)
Isolation of single cells from individual mouse ovaries for flow cytometry and functional analysis
STAR Protocols
December 1, 2023
Enitome E. Bafor, Toni Martin, Jeffrey Carrell, Megan Karwan, Adrienne E. Kimmel, Uyi.G. Omogiade, Michael Sanford, Howard A. Young, Julio C. Valencia
90 μL of FACS buffer. Use immediately.•Viability dye (Zombie Aqua Fixable Viability Kit,
BioLegend
#
423102
).•RPMI medium.CRITICAL: Prepare FBS under sterile conditions. Filter the solution through a 75 mm rapid
(10)
Discovery of a new marker to identify myeloid cells associated with metastatic breast tumours
Cancer Cell International
November 18, 2023
Ansooya A. Bokil, Mathieu Le Boulvais Børkja, Camilla Wolowczyk, Apsana Lamsal, Wenche S. Prestvik, Unni Nonstad, Kristine Pettersen, Sonja B. Andersen, Anna M. Bofin, Geir Bjørkøy,
et al.
the cell suspensions were washed twice, and samples were stained with Zombie Aqua (
BioLegend
, Cat #
423101
) for 10 min on ice, according to the manufacturer’s instructions. After surface
staining
, cells were
(11)
A rat liver cell atlas reveals intrahepatic myeloid heterogeneity
iScience
November 17, 2023
Delaram Pouyabahar, Sai W. Chung, Olivia I. Pezzutti, Catia T. Perciani, Xinle Wang, Xue-Zhong Ma, Chao Jiang, Damra Camat, Trevor Chung, Manmeet Sekhon,
et al.
RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Zombie Aqua™ Fixable Viability Kit,
BioLegend
,Cat#
423101
Mouse anti-rat CD45 BV786;
Clone
: OX-1,
BD Biosciences
,Cat#
740914
; RRID: AB_2740556 Mouse anti-rat
(12)
Mice with FVB-derived sequence on chromosome 17 succumb to disseminated virus infection due to aberrant NK cell and T cell responses
iScience
November 17, 2023
Taylor N. Tibbs, Lauren J. Donoghue, Ashlyn A. Buzzelli, Ichiro Misumi, Maggie DeMonia, Martin T. Ferris, Samir N.P. Kelada, Jason K. Whitmire
enzyme and buffer,
ThermoFisher Scientific
,Cat#
ER0911
Zombie Aqua Fixable Viability Dye,
Biolegend
,Cat#
423101
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,,
Foxp3
Permeabilization/Fixation
(13)
Resveratrol Attenuates 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Mediated Induction of Myeloid-Derived Suppressor Cells (MDSC) and Their Functions
Nutrients
November 3, 2023
Wurood Hantoosh Neamah, Alex Rutkovsky, Osama Abdullah, Kiesha Wilson, Ryan Bloomquist, Prakash Nagarkatti, Mitzi Nagarkatti
Kits were purchased from StemCell Technologies (Vancouver, Canada). Zombie Aqua Fixable Viability Kit (
423102
) was purchased from
BioLegend
. The XFp glycolytic rate
assay
kit was purchased from Agilent Technologies
(14)
PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models
The Journal of clinical investigation
November 1, 2023
Baldrighi M, Doreth C, Li Y, Zhao X, Warner E, Chenoweth H, Kishore K, Umrania Y, Minde DP, Thome S,
et al
.
counter (Chemometec). Collected cell samples were then stained with Zombie Aqua viability dye (
BioLegend
,
423102
) for 10 minutes at room temperature in the dark. Then, samples were stained with the desired antibodies
(15)
Iron overload induces cerebral endothelial senescence in aged mice and in primary culture in a sex‐dependent manner
Aging Cell
November 1, 2023
Brian Noh, Maria Pilar Blasco‐Conesa, Syed Mushfiqur Rahman, Sheelu Monga, Rodney Ritzel, Gary Guzman, Yun‐Ju Lai, Bhanu Priya Ganesh, Akihiko Urayama, Louise D. McCullough,
et al.
#
124010
). The fixable viability dye Zombie Aqua was used for live/dead discrimination (
Biolegend
, #
423102
). Cells were then washed in FACS buffer, fixed in 2% paraformaldehyde for 8 m, and washed once more
(16)
Immune synapse formation promotes lipid peroxidation and MHC-I upregulation in licensed dendritic cells for efficient priming of CD8+ T cells
Nature Communications
October 25, 2023
Diego Calzada-Fraile, Salvador Iborra, Marta Ramírez-Huesca, Inmaculada Jorge, Enrico Dotta, Elena Hernández-García, Noa Martín-Cófreces, Estanislao Nistal-Villán, Esteban Veiga, Jesús Vázquez,
et al.
for flow cytometry
. In this case, the live/dead probe used was Zombie Aqua Fixable Viability Kit (Cat#
423101
,
BioLegend
). For biotin detection, an anti-biotin PE antibody (in MHC-I) or Streptavidin-BV421 (in lipid
(17)
Features and protective efficacy of human mAbs targeting Mycobacterium tuberculosis arabinomannan
JCI Insight
October 23, 2023
Yanyan Liu, Tingting Chen, Yongqi Zhu, Aisha Furey, Todd L. Lowary, John Chan, Stylianos Bournazos, Jeffrey V. Ravetch, Jacqueline M. Achkar
phycoerythrin-labeled streptavidin (
405204
,
BioLegend
) to identify the AM-positive cells. Zombie Aqua (
423101
,
BioLegend
) was included to exclude dead cells. Single live B cells that were AM+
CD3
–
CD14
–
CD19
+
CD27
+IgG+
(18)
Conditional blastocyst complementation of a defective Foxa2 lineage efficiently promotes the generation of the whole lung
eLife
October 20, 2023
Akihiro Miura, Hemanta Sarmah, Junichi Tanaka, Youngmin Hwang, Anri Sawada, Yuko Shimamura, Takehiro Otoshi, Yuri Kondo, Yinshan Fang, Dai Shimizu,
et al.
Epcam
-BV711 (
BioLegend
,
118233
, 1/50), or
Epcam
-BV421 (
BioLegend
,
118225
, 1/50), Aqua Zombie (
BioLegend
,
423101
, 1/100),
CD45
-BV605 (
BioLegend
,
103104
, 1/50) for 30 min on ice. After
staining
, cells were washed twice
(19)
Mitochondrial transplantation ameliorates doxorubicin-induced cardiac dysfunction via activating glutamine metabolism
iScience
October 20, 2023
Xiaolei Sun, Hang Chen, Rifeng Gao, Ya Huang, Yanan Qu, Heng Yang, Xiang Wei, Shiyu Hu, Jian Zhang, Peng Wang,
et al.
APC anti-mouse Ly-6G
Antibody (
BioLegend
) (Cat#
127614
), Zombie Aqua Fixable Viability Kit (
BioLegend
) (Cat#
423102
). The obtained results were expressed as the percent or
cell number
per microgram of tissue.
Flow cytometric analysis
(20)
Intravenous BCG vaccination reduces SARS-CoV-2 severity and promotes extensive reprogramming of lung immune cells
iScience
October 20, 2023
Singh AK, Wang R, Lombardo KA, Praharaj M, Bullen CK, Um P, Gupta M, Srikrishna G, Davis S, Komm O,
et al
.
were washed again using ice-cold PBS and stained using Zombie Aqua™ Fixable Viability Kit (
Biolegend
;
423101
) for 20 min at room temperature. Cells were subsequently washed and resuspended in FACS buffer (1% BSA,
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Reviews
Write a Review
(1)
1/22/2024
Reliable Live Dead Dye From BioLegend with Cell Fixation
laura denney
I used Zombie Viability dyes to examine dead cell frequency in flow cytometry samples. Samples needed to be fixed with formaldehyde so I used a live/dead dye which allows fixation.
(1)
1/1/2024
BioLegend's Fixable Kit for the Distinction of Live and Dead Cells
amirah al jawazneh
I used the Zombie Aqua Fixable Viability Kit in both human and mouse cells. The product works great and it is available in different fluorochromes (to be adapted to every FACS panel). The staining ...
Read Review
(1)
11/10/2020
Good Fixable Viability Dye
Anonymous Review
If you need to fix your cells, this fixable dye allows you to stain them for viability first and then fix them. Here I was checking the percentage of Tregs in my cell population so I needed to fix/...
Read Review
(1)
4/20/2020
Blue Zombies
Gregor Werba
Easy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.
(1)
4/4/2018
Clear Identification Of Dead Cells
Chris Corkum
Our lab routinely utilizes flow cytometry to investigate our research questions. Eliminating dead cells from flow cytometric data is necessary to obtain quality results, especially when working with ...
Read Review
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Zombie Aqua(TM) Fixable Viability Kit from BioLegend
Supplier Page
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