Zombie Aqua™ Fixable Viability Kit from BioLegend

Product Specs
ItemZombie Aqua™ Fixable Viability Kit
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number423101
Quantity100T
ApplicationsFC,ICFC,ICC

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A commensal protozoan exacerbates acetaminophen-induced liver injury by producing free sphingosineGut MicrobesDecember 31, 2026Xiaoqing Yan, Yusi Shen, Qian Sun, Xiuwen Zhu, Qiuchong Chen, Longxiang Liao, Yuan Zhou, Wanpeng Cheng, Zhen Shi, Zhuanzhuan Liu, et al.with APAP plus sphingosine or TMAO for 24 h, and stained using the Zombie Aqua™ Fixable Viability Kit (423101, Biolegend). For western blot assay, cells were treated with APAP plus sphingosine or TMAO for 12 h,

(2) A NANOBODY molecule that blocks MerTK ectodomain cleavage in vitro and in vivoMAbsDecember 31, 2026Linde Duprez, Ayse Kilic, Hélène Bruwiere, Karim Fekir, Dennis Vlaeminck, Camille De Craene, Daan Geiregat, Kirsten Paesen, Lien Leutenez, Stijn Lambrecht, et al.supplemented with 30 µM HSA. Cells were washed and stained with Zombie Aqua Viability Dye (Biolegend, cat. no. 423101/423102), human Fc-block and mouse anti-FLAG (Sigma-Aldrich, cat. no. F1804) or ABH0074 and anti-mouse

(3) DNA prime and peptide boost immunization elicits robust neoantigen-specific CD8 + T cell responses and therapeutic protection in mouse tumor modelsOncoImmunologyDecember 31, 2026Pablo Morgado-Cáceres, Francisca Hofmann-Vega, Diego Figueroa, Juan Saavedra-Almarza, Felipe Gálvez-Cancino, Ximena Díaz, Evelyn Menares, Eduardo Roa, Sofia Hidalgo, Manuel Varas-Godoy, et al.in PBS-FBS 2% by 10 min at 4 °C, washed twice with PBS, incubated with Zombie AquaTM (Biolegend, ref 423102) viability dye diluted in PBS by 20 min at 4 °C, washed twice with PBS, incubated with the antibodies

(4) Fusobacterium nucleatum-derived succinic acid aggravates colitis by triggering macrophage pro-inflammatory phenotypic transformation via SUCNR1/NF-κB axisGut MicrobesDecember 31, 2026Suqi Zeng, Shanshan Jiang, Jianxuan Sun, Yan Chen, Meiqi Qiu, Jiao Li, Qingzhi Lan, Yu Pu, Zongbiao Tan, Xingzhou Guo, et al.were stained according to the respective antibody manufacturers’ protocols. Zombie Aqua viability dye (423102, BioLegend, USA) was used to discriminate live and dead cells. For BMDM, the same staining protocol

(5) "Locking" CDK9 inhibition by activating integrator function in HIV transcriptioniScienceNovember 20, 2026Zichong Li, Francisco J. Zapatero-Belinchón, Patrik Geleziunas, Melanie OttEasySep Human CD4+ T Cell Isolation Kit,STEMCELL,17952 LIVE/DEAD Cell Stain Kit,Invitrogen,L34955 Zombie Aqua Fixable Viability Kit,BioLegend,423101 ,, Deposited data,Deposited data,Deposited data ,, Transcriptomic effects of combined AZD4573 and

(6) Chronic stress drives tumorigenesis and progression via central nervous system-mediated neuroimmune circuitsInt J Oral SciOctober 30, 2026Zhixin Yang, Tianqing Wang, Xin Tian, Silu Sun, Shuyu Xiang, Yuting Hu, Dan Yang, Ying Wang, Lin Ye, Hao Xu, et al.were washed and resuspended in FACS buffer. Cells were stained with LIVE/DEAD fixable viability dye (423101, BioLegend), CD11b‑FITC (101205, BioLegend), Ly6C‑RB780 (568739, BD Pharmingen), and Ly6G‑APC (127613,

(7) MEK1/2 Signalling establishes an alloreactive effector T cell program through SMAD4 during cardiac allograft rejectionClin Transl MedOctober 27, 2026Jiulu Zhao, Shuan Ran, Sijie Zhou, Yuan Li, Wang Zhan, Longyong Lai, Jikai Cui, Jiahong Xia, Jie Wu30 min at 4°C. Dead cells were identified and excluded using the Zombie Aqua Fixable Viability Kit (423102, BioLegend). Following surface staining, intracellular Ki‐67 and FOXP3 were detected using the intracellular/transcription

(8) CREBBP loss drives immune evasion by epigenetically silencing STING and MHC-I in small cell lung cancerSci AdvOctober 23, 2026Qing Gao, Kehong Wei, Deshen Pan, Chaoliang Xu, Yufei Xi, Yan Zhang, Arnaud Augert, David MacPherson, Deshui Jiasubcutaneous allograft tumors. One to two million cells were stained with Zombie Aqua Fixable Viability Dye (423102, BioLegend). After Fc-receptor blockade with Mouse BD Fc Block (553141, BD Biosciences, RRID: AB_394656),

(9) Inverse Vaccine Nanomimics: In situ Antigen-Specific Immune Tolerance Induction for Durable Cardiac Repair Post-Myocardial InfarctionResearch (Wash D C)October 21, 2026Tian Yue, Jiali Yang, Qing Ma, Jian He, Ying Wang, Zhilu Yang, Jun Houantibody-labeling protocol for FC was as follows: CD16/32 (E-AB-F0997A, Elabscience, China), Zombie Aqua (423101, BioLegend, USA), CD11c-APC (E-AB-F0991E, Elabscience, China), CD80-FITC (E-AB-F0992C, Elabscience,

(10) Type I IFN promotes ILC2-to-ILC1 plasticity in pre-clinical radiation-induced lung injuryNat CommunOctober 20, 2026Jaeho Kim, Haiqing Li, Rifat Mannan, Taylor A. Doherty, Heather M. McGeeremove red blood cells. Cells were stained with Zombie Aqua Fixable Viability Dye (BioLegend, Cat# 423102), followed by Fc receptor blocking using anti-mouse CD16/32 (clone 93, BioLegend, Cat# 101302). Surface

(11) P2X7R+CD8αβ+ T cells exacerbate postoperative ileus by inducing the loss of enterochromaffin cellsiScienceOctober 16, 2026Fandi Bu, Shiyang Huang, Xiaobao Yang, Luyang Wei, Yingchi Yang, Zhongtao Zhang, Dong Zhang, Dan Tianincubated at 37°C for 30 min. Flow cytometry Zombie Aqua™ Fixable Viability Kits (Biolegend, CAT# 423102) were used to exclude dead cells. Antibody staining was performed at 4°C with fluorochrome-conjugated

(12) ApolipoproteinE+ Kupffer cells maintain immune homeostasis following liver transplantation by inducing CD8+ T cell exhaustioniScienceOctober 16, 2026Zhuoyu Jia, Xinqiang Li, Xueteng Wang, Xin Zhou, Hailun Cai, Peng Jiang, Ruidong Ding, Huan Liu, Ye Wang, Shipeng Li, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Zombie Aqua™ Fixable Viability Kit,BioLegend,423101 Anti-CD45,BioLegend,Cat# 304018; RRID: AB_389336 Anti-CD11b,BioLegend,Cat# 301402; RRID: AB_314166 Anti-F4/80,BioLegend,Cat#

(13) Cancers modulate processing and presentation of p53 neoantigens to evade T cell detectionImmunityOctober 13, 2026Koji Haratani, Bruce Reinhold, Jonathan S. Duke-Cohan, Caroline G. Fahey, Kemin Tan, Robert J. Mallis, Alexander Gusev, Kenneth L. Kehl, Jia Luo, Allyson Karmazyn, et al.once (450g, 6 mins), followed by incubation in PBS with Zombie-NIR (423106, BioLegend), Zombie-Aqua (423102, BioLegend), or Zombie-Green (423112, BioLegend) at 0.1:50 at RT for 5 mins. The plates were then spun

(14) ADAM17-mediated CD16 shedding drives monocyte subset misclassification in ex vivo immunological assaysFront ImmunolOctober 13, 2026Soyeon Kwon, Seung Kak Shin, Seungwon RyuPBS. To exclude dead cells, cells were first stained with the Zombie Aqua Fixable Kit (Biolegend, Cat# 423102) for 5 minutes on ice in the dark. Cells were then washed with staining buffer consisting of PBS supplemented

(15) 4-Octyl itaconate inhibits synovial fibroblast activation by targeting Nrf2 to exert anti-inflammatory effects in rheumatoid arthritisEULAR Rheumatology OpenOctober 10, 2026Benedicte B. Andersen, Samuel Kemble, Stinne Greisen, Jacob Thyrsted, Vivien R. Schack, Elisabeth Fuchs, Anne L. Hansen, Thomas B. Poulsen, Christian K. Holm, David Olagnier, et al.method. In flow cytometry, fibroblast engagement was evaluated using the gating strategy Live (BioLegend #423102), CD45-(BioLegend #103147), CD31-(BioLegend #102418) and PDPN+ (BioLegend #127408). Collagen-induced arthritis

(16) Functional Divergence of Regulatory and Conventional Bovine Neutrophils following Mycobacterium bovis InfectionJournal of Innate ImmunityOctober 9, 2026Maïa Saint-Vanne, Badreddine Bounab, Sebastien Eymieux, Emma Perdriau, Patricia Cunha, Florence Carreras, Romain Roullier, Yves Le Vern, Julien Pichon, Emilie Doz-Deblauwe, et al.eFluor 780,–,–,"1:10,000",65-0865-14,Thermo Fisher Scientific Viability dye Zombie Aqua,–,–,1:500,423102,BioLegend Sulforhodamine-FLICA Polycaspase,–,–,1:250,ICT916,Bio-Rad CellROX Green,–,–,5 µm,C10444,Thermo Fisher Scientific

(17) Kupffer cells are key mediators of antigen-specific immune tolerance by peptide-coupled red blood cellsSci AdvOctober 9, 2026Vasileia Kalaitzaki, Pietro Oldrati, Filipa M. Ferreira, Antonios Katsoulas, Ahmed Ali, Livio Baselgia, Yun Liu, Jincheng Fang, Roman Sankowski, Mai Matsushita, et al.centrifugation, the cell pellet was resuspended in 100 μl of Zombie NIR Fixable Viability Kit (1:1000 in PBS; #423102, BioLegend) and stained for 20 min (4°C in the dark). After topping up with PBS, the cells were washed,

(18) Maternal-fetal type I interferon signaling drives TREM2 dysregulation and synaptic dysfunction in neurodevelopmental disordersNeuronOctober 7, 2026Matteo Bizzotto, Shih Feng You, Matteo Tamborini, Elisa Faggiani, Raffaella Morini, Antonella Borreca, Rui Zhang, Ekaterina Aladyeva, Aasritha Nallapu, Claudio Ferrari, et al.CD11bhighCD45int and CX3CR1+P2yr12+ cells. The dead cells were gated out by staining with zombie aqua (cat. 423102, Biolegend). Appropriate antibody IgG isotype controls were used for all staining. FACS analysis was

(19) GULP1 Fuels Resistance to PD-1 Blockade through Lipid-Mediated Metabolic Reprogramming of Tumor-Associated Macrophages in Gastric CancerCancer CommunicationsOctober 6, 2026Zihao Zhang, Wenxin Xu, Kuan Yu, Anran Bao, Yifan Cao, Mincheng Yu, Qiang Yu, Yufei Zhao, Liang Gong, Peiyi Xie, et al.detached with trypsin and subsequently resuspended in PBS. After labeling with a fixable viability dye (423101, Zombie Aqua Fixable Viability Kit, BioLegend), the cells were fixed and permeabilized using the Fixation and Permeabilization Solution

(20) Bone targeted microenvironment actuated engineered exosomes for precision therapy of IBD associated bone lossBioactive MaterialsOctober 1, 2026Jiang Liu, Cong Wang, Taojin Feng, Siyu Chen, Siliang Ge, Yue Zhao, Ming Chen, Zhongqi Wang, Weier Bao, Pincong Fu, et al.in the presence of brefeldin A. The cells were then stained with Zombie Aqua Fixable Viability Kit (423102, BioLegend) to exclude dead cells and with FITC anti-mouse CD4 (100406, BioLegend) antibody for surface

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(1)

1/22/2024Reliable Live Dead Dye From BioLegend with Cell Fixation laura denneyI used Zombie Viability dyes to examine dead cell frequency in flow cytometry samples. Samples needed to be fixed with formaldehyde so I used a live/dead dye which allows fixation.
(1)

1/1/2024BioLegend's Fixable Kit for the Distinction of Live and Dead Cells amirah al jawaznehI used the Zombie Aqua Fixable Viability Kit in both human and mouse cells. The product works great and it is available in different fluorochromes (to be adapted to every FACS panel). The staining ... Read Review
(1)

11/10/2020Good Fixable Viability Dye Anonymous ReviewIf you need to fix your cells, this fixable dye allows you to stain them for viability first and then fix them. Here I was checking the percentage of Tregs in my cell population so I needed to fix/... Read Review
(1)

4/20/2020Blue Zombies Gregor WerbaEasy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.
(1)

4/4/2018Clear Identification Of Dead Cells Chris CorkumOur lab routinely utilizes flow cytometry to investigate our research questions. Eliminating dead cells from flow cytometric data is necessary to obtain quality results, especially when working with ... Read Review

Zombie Aqua™ Fixable Viability Kit from BioLegend

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Zombie Aqua™ Fixable Viability Kit from BioLegend

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