Zombie Aqua™ Fixable Viability Kit 423101 from BioLegend

Product Specs
ItemZombie Aqua™ Fixable Viability Kit
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number423101
Quantity100 tests
ApplicationsFC - Quality tested, ICFC, ICC - Verified

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) DNA prime and peptide boost immunization elicits robust neoantigen-specific CD8 + T cell responses and therapeutic protection in mouse tumor modelsOncoImmunologyDecember 31, 2026Pablo Morgado-Cáceres, Francisca Hofmann-Vega, Diego Figueroa, Juan Saavedra-Almarza, Felipe Gálvez-Cancino, Ximena Díaz, Evelyn Menares, Eduardo Roa, Sofia Hidalgo, Manuel Varas-Godoy, et al.in PBS-FBS 2% by 10 min at 4 °C, washed twice with PBS, incubated with Zombie AquaTM (Biolegend, ref 423102) viability dye diluted in PBS by 20 min at 4 °C, washed twice with PBS, incubated with the antibodies

(2) Regulatory T cells sabotage anti-tumor γδ T cells by creating IL-2-deficient environmentsJ Exp MedJuly 6, 2026Rafael Blanco-Domínguez, André Miguel Vaz-Pinto, Leandro Barros, Noella Lopes, Beatriz Henriques-Alves, Mariana Carreira, Carlos Labão-Almeida, Julie C. Ribot, Gonçalo J.L. Bernardes, Bruno Silva-Santos, et al.,BD Biosciences,562077 ,Viability,Zombie Yellow,500,,BioLegend,423103 ,Viability,Zombie Aqua,500,,BioLegend,423101 List of antibodies and dyes used for flow cytometry analyses

(3) Protein-Dependent CD4+ T Cell Specialization and CD8+ T Cell–Driven IL-2 Production in HIViScienceJune 19, 2026Jernej Pušnik, Falko M. Heinemann, Andreas Heinold, Enrico Richter, Stefan Esser, Hendrik StreeckStimulated cells were then washed with PBS and subsequently stained for viability (ZombieAqua, Biolegend, 423102) for 15 min at 4°C. Next, cells were washed with FACS buffer (PBS supplemented with 2% FCS, 0.05% NaN3,

(4) Alveolar Macrophages Undergo Postnatal Transition from a Hyperphagocytic and Proinflammatory Profile to a Tolerogenic ProfileiScienceJune 19, 2026Hang Yu, Shengwen Shang, Qing Xu, Jiabing Tong, Jian Wang, Fengqi Lianti-mouse Ly6G,BioLegend,Cat# 127622; RRID: AB_10643269 Zombie Aqua™ Fixable Viability Kit,BioLegend,Cat#423102; N/A ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(5) Targeting ADAR1 p150 triggers tumor inhibition and antitumor immunity to overcome immunotherapy resistanceiScienceJune 19, 2026Zhichao(Eric) Ai, Yuying Bian, Gang Li, Xiaobin S. Wang, Sining Zhu, Yumeng Li, Xiangyu Yao, Zhipeng Yangradient. Cells were stained with a live/dead viability dye (Zombie Aqua™ Fixable Viability Kit, BioLegend, 423102) followed by surface antigen staining. For intracellular transcription factor and cytokine staining,

(6) Nicotinamide mononucleotide enhances anti-tumor effect by resetting macrophages toward the inflammatory M1-like phenotypeMol Ther OncolJune 18, 2026Haoran Xu, Marcus Chun Tao Wan, Chelsey Chi Ching Wong, Siqi Qin, Yang Wen, Jun Wang, Zhiwei ChenPacific Blue anti-mouse CD4 (Clone: RM 4–5, Cat# 48-0042-82), Zombie Aqua Fixable Viability Kit (BioLegend, Cat# 423102). For tetramer staining assay, PE-conjugated H-2Kd HIV-1 Gag AMQMLKETI (AI9) MHC class I tetramer (MBL)

(7) Protocol for assessing anti-Siglec-9 antibody-mediated tumor phagocytosis by macrophages using pH-sensitive dyes and flow cytometrySTAR ProtocMay 20, 2026Saitong Muneekaew, Thunchanok Nuchphongsai, Pasut Sasithong, Kritayaporn Saiprayong, Kamonphan Rungrueang, Methichit Wattanapanitchdilution),BioLegend,Cat. No. 351502 Zombie Aqua™ Fixable viability dye (1:500 dilution),Biolegend,Cat. No. 423102 Human TruStain FcX (TM) (1:20 dilution),BioLegend,Cat. No. 422302 True-Stain Monocyte Blocker (1:20

(8) High-quality acinar cell isolation enables single-cell analysis of healthy and injured pancreasCell Reports MethodsMay 18, 2026Nirav Florian Chhabra, Leeanne J. Mundle, Henrik Einwächter, Roland M. Schmidblocking,Biolegend,Cat# 101319; RRID: AB_1574973 Zombie Aqua™ Fixable Viability Kit,Biolegend,Cat# 423101 CD3ε BUV395,BD Biosciences,Cat# 565992; RRID: AB_2739443 CD19 FITC,Biolegend,Cat# 115505; RRID: AB_313640 CD45

(9) Identifying Donor Immune Cells in the Intestinal Mucosa of Chimeric Recipient Mice by ImmunofluorescenceCurr ProtocMay 18, 2026Vinicius Canale, Samuel Asiedu, Fuad Malki, Gang Chen, Meera G. Nair, Declan F. McColeefficiency of BMT. To assess cell viability, cells were stained with Zombie Aqua (BioLegend, cat. #423102) and incubated for 15 min at room temperature in the dark. Fc receptors were then blocked by incubating

(10) Evaluation of Safety and Immunogenicity of High-Dose Quadrivalent Seasonal Influenza Split Vaccine: A Preclinical StudyVaccines (Basel)May 17, 2026Lanxin Jia, Ran Qiu, Jing Liu, Bo Liu, Xuanxuan Nian, Yang Le, Xixin Han, Qingmei Zhang, Xuedan Li, Zheng Gong, et al.for viability using the Zombie Aqua™ Fixable Viability Kit (BioLegend, San Diego, CA, USA; Cat. No. 423102) for 15 min at room temperature in the dark. After staining, cells were washed with Cell staining buffer

(11) Hypoxia Impairs CD8+ T Cell Fitness and Is Associated with a Dysfunctional CD8+ T Cell State in Pancreatic CancerCancers (Basel)May 8, 2026Ashley M. Mello, Marina Pasca di Magliano, Kyoung Eun Leefluorophore-conjugated antibodies for 30 min on ice. Dead cells were excluded by staining with a fixable viability dye (#423102, BioLegend) for 15 min at room temperature. Intracellular staining was carried out using the True-Nuclear Transcription Factor Buffer Set

(12) Engulfment by brain macrophages in a short-lived vertebratebioRxivMay 5, 2026Rahul Nagvekar, Angela N. Pogson, Prateek R. Kalakuntla, Helena J. Barr, Azalia M. Martínez Jaimes, S.V. Perry, Emma K. Costa, Jingxun Chen, Felix Boos, Paloma Navarro Negredo, et al.aspirated and discarded, and the pellet was resuspended in 500 μL PBS with Zombie Aqua™ (BioLegend, 423101) diluted 1:1000 to generate the single-cell suspension. Flow cytometry and data analysis for ex vivo

(13) Identification of characteristic hub genes in the immune-active phase of chronic hepatitis BFront ImmunolMay 4, 2026Fangfang Li, Yi Li, Yirong Du, Chao Fan, Jun Xiong, Fei Hu, Xiaoying Deng, Xiaoyan Wang, Hongyan Shi, Xiangwei Sun, et al.conducted doublet exclusion and live cell gating (using Zombie Aqua™ Fixable Viability Dye, BioLegend, Cat# 423102). CD14/CD16 gating was conducted. CD45+ cells were gated in advance using CD45 antibody (CD45 monoclonal

(14) Intratumoral Virus-Like Particles Containing a TLR9 Agonist Combined with Systemic αPD-1 Activate Tumor-Specific CD8+ T CellsCancer Res CommunMay 1, 2026Travis D. Fischer, Caitlin D. Lemke-Miltner, George J. Weinerevaluation. Cells were collected and stained with Zombie Aqua Fixable Viability dye (BioLegend, cat. #423101) or Live/Dead Blue Viability dye (Thermo Fisher Scientific, cat. #L23105) according to the manufacturers’

(15) Intratumoral Virus-Like Particles Containing a TLR9 Agonist Combined with Systemic αPD-1 Activate Tumor-Specific CD8+ T CellsCancer Res CommunMay 1, 2026Travis D. Fischer, Caitlin D. Lemke-Miltner, George J. Weinerevaluation. Cells were collected and stained with Zombie Aqua Fixable Viability dye (BioLegend, cat. #423101) or Live/Dead Blue Viability dye (Thermo Fisher Scientific, cat. #L23105) according to the manufacturers’

(16) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,VECTASHIELD Vibrance Antifade Mounting Medium,Vector Laboratories,H-1700-10, "Chemical compound, drug",Zombie Aqua Fixable Viability Kit,BioLegend,423101, "Chemical compound, drug",Zombie NIR Fixable Viability Kit,BioLegend,423105, "Chemical compound,

(17) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrowElifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livak, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,VECTASHIELD Vibrance Antifade Mounting Medium,Vector Laboratories,H-1700-10, "Chemical compound, drug",Zombie Aqua Fixable Viability Kit,BioLegend,423101, "Chemical compound, drug",Zombie NIR Fixable Viability Kit,BioLegend,423105, "Chemical compound,

(18) Homeostatic mature dendritic cells instruct fibroblast specialization via Notch2 signaling to establish T cell nichesImmunityApril 23, 2026Nagham Alouche, Hélène Cannelle, Stéphanie Favre, Marija Jokić, José A. Villegas, Sandra Maréchal, Clint De Nolf, Maeva Delacrétaz, Alexandros Sifis, Chloé Chapuis, et al.assays,Critical commercial assays,Critical commercial assays Zombie Aqua Fixable Viability Kit,BioLegend,Cat# 423101 Foxp3/Transcription Factor Staining Buffer Set,eBioscience,Cat# 00-5523-00 BD Cytofix/Cytoperm™ Fixation/Permeabilization

(19) TAF1 Suppresses MHC-I Expression and Correlates with Poor Immunotherapy Response in Small Cell Lung CancerBiomedicinesApril 23, 2026Qing Gao, Kehong Wei, Deshen Pan, Yufei Xi, Chaoliang Xu, Deshui Jiafirst stained for viability with Zombie Aqua Fixable Viability Dye (BioLegend, San Diego, CA, USA, #423102) and then blocked with Mouse BD Fc Block (BD Biosciences, San Jose, CA, USA, #553141). Surface staining

(20) Cationic nanoparticles with disrupting neutrophil extracellular traps inhibit the progression of head and neck squamous cell carcinomaFrontiers in Cell and Developmental BiologyApril 22, 2026Zhaoqiang Zhang, Yujie Kang, Bingxu Lu, Guichao Zhang, Baohan Xie, Yunyi Wang(Catalog No. BA1054; Boster Biological Technology, China), Zombie Aqua Fixable Viability Kit (Catalog No. 423102, BioLegend), APC anti-mouse CD19 Antibody (Catalog No. 115512, BioLegend), Brilliant Violet 421 anti-mouse

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(1)

1/22/2024Reliable Live Dead Dye From BioLegend with Cell Fixation laura denneyI used Zombie Viability dyes to examine dead cell frequency in flow cytometry samples. Samples needed to be fixed with formaldehyde so I used a live/dead dye which allows fixation.
(1)

1/1/2024BioLegend's Fixable Kit for the Distinction of Live and Dead Cells amirah al jawaznehI used the Zombie Aqua Fixable Viability Kit in both human and mouse cells. The product works great and it is available in different fluorochromes (to be adapted to every FACS panel). The staining ... Read Review
(1)

11/10/2020Good Fixable Viability Dye Anonymous ReviewIf you need to fix your cells, this fixable dye allows you to stain them for viability first and then fix them. Here I was checking the percentage of Tregs in my cell population so I needed to fix/... Read Review
(1)

4/20/2020Blue Zombies Gregor WerbaEasy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.
(1)

4/4/2018Clear Identification Of Dead Cells Chris CorkumOur lab routinely utilizes flow cytometry to investigate our research questions. Eliminating dead cells from flow cytometric data is necessary to obtain quality results, especially when working with ... Read Review

Zombie Aqua™ Fixable Viability Kit from BioLegend

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Zombie Aqua™ Fixable Viability Kit from BioLegend

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