Zombie Aqua™ Fixable Viability Kit 423101 from BioLegend

Product Specs
ItemZombie Aqua™ Fixable Viability Kit
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number423101
Quantity100T
ApplicationsFC,ICFC,ICC

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Fusobacterium nucleatum-derived succinic acid aggravates colitis by triggering macrophage pro-inflammatory phenotypic transformation via SUCNR1/NF-κB axisGut MicrobesDecember 31, 2026Suqi Zeng, Shanshan Jiang, Jianxuan Sun, Yan Chen, Meiqi Qiu, Jiao Li, Qingzhi Lan, Yu Pu, Zongbiao Tan, Xingzhou Guo, et al.were stained according to the respective antibody manufacturers’ protocols. Zombie Aqua viability dye (423102, BioLegend, USA) was used to discriminate live and dead cells. For BMDM, the same staining protocol

(2) A commensal protozoan exacerbates acetaminophen-induced liver injury by producing free sphingosineGut MicrobesDecember 31, 2026Xiaoqing Yan, Yusi Shen, Qian Sun, Xiuwen Zhu, Qiuchong Chen, Longxiang Liao, Yuan Zhou, Wanpeng Cheng, Zhen Shi, Zhuanzhuan Liu, et al.with APAP plus sphingosine or TMAO for 24 h, and stained using the Zombie Aqua™ Fixable Viability Kit (423101, Biolegend). For western blot assay, cells were treated with APAP plus sphingosine or TMAO for 12 h,

(3) DNA prime and peptide boost immunization elicits robust neoantigen-specific CD8 + T cell responses and therapeutic protection in mouse tumor modelsOncoImmunologyDecember 31, 2026Pablo Morgado-Cáceres, Francisca Hofmann-Vega, Diego Figueroa, Juan Saavedra-Almarza, Felipe Gálvez-Cancino, Ximena Díaz, Evelyn Menares, Eduardo Roa, Sofia Hidalgo, Manuel Varas-Godoy, et al.in PBS-FBS 2% by 10 min at 4 °C, washed twice with PBS, incubated with Zombie AquaTM (Biolegend, ref 423102) viability dye diluted in PBS by 20 min at 4 °C, washed twice with PBS, incubated with the antibodies

(4) A NANOBODY molecule that blocks MerTK ectodomain cleavage in vitro and in vivoMAbsDecember 31, 2026Linde Duprez, Ayse Kilic, Hélène Bruwiere, Karim Fekir, Dennis Vlaeminck, Camille De Craene, Daan Geiregat, Kirsten Paesen, Lien Leutenez, Stijn Lambrecht, et al.supplemented with 30 µM HSA. Cells were washed and stained with Zombie Aqua Viability Dye (Biolegend, cat. no. 423101/423102), human Fc-block and mouse anti-FLAG (Sigma-Aldrich, cat. no. F1804) or ABH0074 and anti-mouse

(5) ApolipoproteinE+ Kupffer cells maintain immune homeostasis following liver transplantation by inducing CD8+ T cell exhaustioniScienceOctober 16, 2026Zhuoyu Jia, Xinqiang Li, Xueteng Wang, Xin Zhou, Hailun Cai, Peng Jiang, Ruidong Ding, Huan Liu, Ye Wang, Shipeng Li, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Zombie Aqua™ Fixable Viability Kit,BioLegend,423101 Anti-CD45,BioLegend,Cat# 304018; RRID: AB_389336 Anti-CD11b,BioLegend,Cat# 301402; RRID: AB_314166 Anti-F4/80,BioLegend,Cat#

(6) P2X7R+CD8αβ+ T cells exacerbate postoperative ileus by inducing the loss of enterochromaffin cellsiScienceOctober 16, 2026Fandi Bu, Shiyang Huang, Xiaobao Yang, Luyang Wei, Yingchi Yang, Zhongtao Zhang, Dong Zhang, Dan Tianincubated at 37°C for 30 min. Flow cytometry Zombie Aqua™ Fixable Viability Kits (Biolegend, CAT# 423102) were used to exclude dead cells. Antibody staining was performed at 4°C with fluorochrome-conjugated

(7) Bone targeted microenvironment actuated engineered exosomes for precision therapy of IBD associated bone lossBioactive MaterialsOctober 1, 2026Jiang Liu, Cong Wang, Taojin Feng, Siyu Chen, Siliang Ge, Yue Zhao, Ming Chen, Zhongqi Wang, Weier Bao, Pincong Fu, et al.in the presence of brefeldin A. The cells were then stained with Zombie Aqua Fixable Viability Kit (423102, BioLegend) to exclude dead cells and with FITC anti-mouse CD4 (100406, BioLegend) antibody for surface

(8) The Impact of SF3B1 Mutations on the Tumor Microenvironment and Response to ImmunotherapyCancer MedSeptember 28, 2026Kyung-Jin Cho, Byungho LimDead cells were excluded from the analysis using a fixable viability dye (Zombie Aqua; BioLegend, Cat#423101), and all flow cytometry data were gated on live cells. Flow cytometry analysis was performed using

(9) AI-Designed TREM1-Targeted LYTAC Nanoparticles Reprogram the Neuroimmune Microenvironment in Traumatic Brain InjuryAdv Sci (Weinh)September 27, 2026Yimin Huang, Yuan Liu, Huan Peng, Yu Ni, Zhengqiao Jiang, Chenxuan Yu, Xincheng Zhang, Huayu Kang, Yanchao Liu, Juan Chen, et al.(TreeStar). To exclude dead cells and cell aggregates, the Zombie Aqua Fixable Viability Kit (Cat# 423101, BioLegend, California, USA) and FSC‐H/FSC‐A characteristics were used. 5.23 EdU Staining Experiment Upon

(10) CXCL3 Antagonism Reverses Systemic Antitumor Immune Memory Impairment Associated With High Tumor Burden and Sensitizes Anti-PD-1 TherapyAdv Sci (Weinh)September 27, 2026Ji Wang, Xinyu Tang, Zeju Li, Qi Qi, Chang Sun, Wen Sun, Weiya Zhang, Yue Yin, Ya Deng, Peiwen Ling, et al.blood immune cells from mice. Resuspended cells were stained with Zombie Aqua Fixable Viability Kit (423101/423102, BioLegend, USA) for 20 min at 25°C in 100 µL of PBS. Cell surface antigens were stained with

(11) High-Density Type I Collagen Promotes IFN-γ+ CD8+ T Cell Exhaustion via SAT1-ASS1-Mediated Glutamine Accumulation and Ferroptosis in Triple-Negative Breast CancerAdv Sci (Weinh)September 27, 2026Jinyan Wang, Shuangyue Pan, Lu Bai, Bin Li, Tiantian Liu, Jianing Cao, Mengdi Yang, Heda Zhang, Duancheng Guo, Wenxiang Zhi, et al.USA) at 37°C for 4 h. The samples were stained with Live/Dead dye (Zombie Aqua Fixable Viability Kit, 423101, Biolegend) for 15 min at room temperature. After being labeled with antibodies against cell surface

(12) A Multi-Targeting Chimeric Antigen Receptor-T Cell Platform to Overcome Antigen Heterogeneity in the Treatment of Non-Small Cell Lung CancerAdv Sci (Weinh)September 27, 2026Lina Wang, Yanlei Zhang, Shuixiu Peng, Chenchen Hou, Huaqi Guo, Tianyu Zhou, Alex H. Chang, Lifeng Yan, Weining XiongBioLegend; and Alexa Fluor 647 anti‐TIM‐3 (FAB2365R), from R&D Systems. Zombie Aqua Live/Dead Staining (Cat# 423101, BioLegend) was utilized to identify viable cells. Isotype‐matched control mAbs were included in all

(13) Mesenchymal Stem Cells Ameliorate Acute-on-Chronic Liver Failure Associated With Expansion of Immunoregulatory Hepatic NKp46+DX5- NK CellsJ Immunol ResSeptember 24, 2026Jing Xiong, Weizhen Weng, Dengna Lin, Huijuan Cao, Shaoquan Zhang, Junfeng Chen, Jing Zhang, Juan Gao, Ildiko Gyory, Zhiliang Gao, et al.(BioLegend #137608), and CD49b (DX5; BioLegend #108907). A fixable viability dye (Zombie; BioLegend #423101) was included to exclude dead cells. NK cell subsets—defined as live (Zombie−), lineage‐negative (CD3−CD19−CD14−),

(14) Systemic delivery of phagocytosis-shielded retroviral vectors enables in vivo HSC gene therapy for sickle cell diseaseCell Stem CellSeptember 24, 2026Denise Klatt, Adele Mucci, Boya Liu, Marie Peschers, Giulia Schiroli, Axel Schambach, John Manis, Myriam Armant, David T. Scadden, Els Verhoeyen, et al.Human TruStain FcX,BioLegend,Cat# 422302 Mouse TruStain FcX,BioLegend,Cat# 101320 Zombie Aqua Viability Dye,BioLegend,Cat# 423102 Critical commercial assays,Critical commercial assays,Critical commercial assays RNeasy® Mini Kit,Qiagen,Cat#

(15) RNF180 loss stabilizes BCAS4 to enhance PD-L1 expression and immune evasion in hepatocellular carcinomaiScienceSeptember 18, 2026Ke Ye, Xiupeng Cai, Xuefan Dongthen FSC-H/FSC-A to exclude doublets. Live cells were identified as Zombie Aqua-negative (BioLegend, #423101), followed by gating on human CD45+ leukocytes and human CD3+ T cells. CD8A identified the total CD8-lineage

(16) Rejuvenating endogenous antitumor immunity via a chimeric receptor-engineered oncolytic virus targeting tumor-associated macrophagesSci AdvSeptember 18, 2026Chaolong Lin, Shaopeng Li, Rui Lou, Wenzhong Teng, Yang Tian, Chenxi Feng, Naizhen Wang, Yipeng Rao, Yaning Qin, Lu Li, et al.CXCL9+) (table S2). The cell suspensions were stained with Zombie Aqua™ Fixable Viability Kit (BioLegend, 423102) for 10 minutes at 4°C. Fc receptors were then blocked using anti-CD16/32 (BD Biosciences, 553141) for

(17) Chronic chikungunya is associated with decreased anti-CHIKV antibody titers, neutralizing capacity, and antibody-dependent cellular cytotoxicityiScienceSeptember 18, 2026Vinit Upasani, Sokchea Lay, Sievleang Heng, Alvino Maestri, Sreymom Ken, Sopheak Sorn, Rithea Leang, Sowath Ly, Veasna Duong, Tineke Cantaertallele",Promega,G7010 NK cell Isolation kit,Miltenyi Biotec,130–092-657 Zombie Aqua™ Fixable Viability Kit,Biolegend,423102 True-Nuclear™ Transcription Factor Buffer Set,Biolegend,424401 EZ-Link Maleimide-PEO Solid-phase Biotinylation

(18) CAR design equips diverse immune cells with unique antigen-specific activation identitiesMolecular Therapy OncologySeptember 17, 2026Ayano Sugiyama-Finnis, Takaharu Kimura, Kiyoko Izawa, Yuko Kawano, Takao Yogo, Hyojung Jeon, Tsuyoshi Fukushima, Ryoji Ito, Satoshi YamazakiA549-CD19+mKate+ cells, staining with Zombie Aqua Fixable Viability Kit (BioLegend, San Diego, California, # 423101) at a 1:500 dilution for 20 min at 4°C was conducted. Following washing in PBS supplemented with 2%

(19) Granzyme B PET Imaging Reveals Lgmn+ Macrophage-Mediated Immune Evasion and Guides Immunotherapy in EGFR-TKI-Resistant NSCLCAdv Sci (Weinh)September 16, 2026Dongliang Wang, Wenqin Luo, Qiufang Liu, Wenxuan Tang, Bin Zhu, Jiwei Chen, Qian Zhou, Shuang Tang, Xiaoping Xu, Shaoli Songwith anti‐CD16/32 antibody (101320, BioLegend, 1:50), and dead cells were labeled using live/dead dye (423102, BioLegend). To quantify the infiltration of cytotoxic T lymphocyte, anti‐CD45 (103134, biolegend, 1:100),

(20) Preclinical evaluation of nitrated α-synuclein-specific CAR-Treg therapy in Parkinson’s diseaseCell Reports MedicineSeptember 15, 2026Valentina Ugalde, Daniela Elgueta, Sandra Espinoza, Ornella Chovar-Vera, Ernesto López, Alejandra Catenaccio, Francisca Álvarez-Astudillo, Dafne Franz, Vanesa Sanchez-Guajardo, María Inés Becker, et al.Foxp3 staining kit,Invitrogen,Cat# 00-5523-00 Zombie Aqua (ZAq) fixable viability kit,Biolegend,Cat# 423102 "NucleoBond Xtra Maxi EF, Maxi kit for endotoxin-free plasmid DNA",Macherey-Nagel,Cat# 740424.10 Imject Maleimide Activated Blue Carrier Protein

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(1)

1/22/2024Reliable Live Dead Dye From BioLegend with Cell Fixation laura denneyI used Zombie Viability dyes to examine dead cell frequency in flow cytometry samples. Samples needed to be fixed with formaldehyde so I used a live/dead dye which allows fixation.
(1)

1/1/2024BioLegend's Fixable Kit for the Distinction of Live and Dead Cells amirah al jawaznehI used the Zombie Aqua Fixable Viability Kit in both human and mouse cells. The product works great and it is available in different fluorochromes (to be adapted to every FACS panel). The staining ... Read Review
(1)

11/10/2020Good Fixable Viability Dye Anonymous ReviewIf you need to fix your cells, this fixable dye allows you to stain them for viability first and then fix them. Here I was checking the percentage of Tregs in my cell population so I needed to fix/... Read Review
(1)

4/20/2020Blue Zombies Gregor WerbaEasy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.
(1)

4/4/2018Clear Identification Of Dead Cells Chris CorkumOur lab routinely utilizes flow cytometry to investigate our research questions. Eliminating dead cells from flow cytometric data is necessary to obtain quality results, especially when working with ... Read Review

Zombie Aqua™ Fixable Viability Kit from BioLegend

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Zombie Aqua™ Fixable Viability Kit from BioLegend

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