Zombie Aqua™ Fixable Viability Kit 423101 from BioLegend

Product Specs
ItemZombie Aqua™ Fixable Viability Kit
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number423101
Quantity100 tests
ApplicationsFC - Quality tested, ICFC, ICC - Verified

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A commensal protozoan exacerbates acetaminophen-induced liver injury by producing free sphingosineGut MicrobesDecember 31, 2026Xiaoqing Yan, Yusi Shen, Qian Sun, Xiuwen Zhu, Qiuchong Chen, Longxiang Liao, Yuan Zhou, Wanpeng Cheng, Zhen Shi, Zhuanzhuan Liu, et al.with APAP plus sphingosine or TMAO for 24 h, and stained using the Zombie Aqua™ Fixable Viability Kit (423101, Biolegend). For western blot assay, cells were treated with APAP plus sphingosine or TMAO for 12 h,

(2) DNA prime and peptide boost immunization elicits robust neoantigen-specific CD8 + T cell responses and therapeutic protection in mouse tumor modelsOncoImmunologyDecember 31, 2026Pablo Morgado-Cáceres, Francisca Hofmann-Vega, Diego Figueroa, Juan Saavedra-Almarza, Felipe Gálvez-Cancino, Ximena Díaz, Evelyn Menares, Eduardo Roa, Sofia Hidalgo, Manuel Varas-Godoy, et al.in PBS-FBS 2% by 10 min at 4 °C, washed twice with PBS, incubated with Zombie AquaTM (Biolegend, ref 423102) viability dye diluted in PBS by 20 min at 4 °C, washed twice with PBS, incubated with the antibodies

(3) Bone targeted microenvironment actuated engineered exosomes for precision therapy of IBD associated bone lossBioactive MaterialsOctober 1, 2026Jiang Liu, Cong Wang, Taojin Feng, Siyu Chen, Siliang Ge, Yue Zhao, Ming Chen, Zhongqi Wang, Weier Bao, Pincong Fu, et al.in the presence of brefeldin A. The cells were then stained with Zombie Aqua Fixable Viability Kit (423102, BioLegend) to exclude dead cells and with FITC anti-mouse CD4 (100406, BioLegend) antibody for surface

(4) Naive CD4+ T cells synthesize a hyaluronan-rich glycocalyx that is diminished following activationiScienceJuly 17, 2026Laxmi Prasad Adhikari, Jessica Contreras, Kristen M. Patterson, Meghan M. Moran, Erin N. Howe, Anna H.K. Plaas, Joseph M. Reynolds80190 Pam3CSK4,InvivoGen,Cat#: tlrl-pms Stains-All,Sigma-Aldrich,Cat#: E9379 Zombie-Aqua Fixable Viability Kit,Biolegend,Cat#: 423102 "Pierce 16% Formaldehyde (w/v), Methanol-free",Thermo scientific,Cat#: 28908 TRIzol Reagent,Thermo Fisher Scientific

(5) Regulatory T cells sabotage anti-tumor γδ T cells by creating IL-2-deficient environmentsJ Exp MedJuly 6, 2026Rafael Blanco-Domínguez, André Miguel Vaz-Pinto, Leandro Barros, Noella Lopes, Beatriz Henriques-Alves, Mariana Carreira, Carlos Labão-Almeida, Julie C. Ribot, Gonçalo J.L. Bernardes, Bruno Silva-Santos, et al.,BD Biosciences,562077 ,Viability,Zombie Yellow,500,,BioLegend,423103 ,Viability,Zombie Aqua,500,,BioLegend,423101 List of antibodies and dyes used for flow cytometry analyses

(6) Preclinical evaluation of cysteine protease-inhibitor aloxistatin (E64d) for heart failure therapyJ Mol Med (Berl)June 25, 2026Maria Jordan, Angelika Stucki-Koch, Maximilian Schinke, Lena Willmer, Ingrid Senne, Naisam Abbas, Marco Bentele, Dana Oldenkott, Nico Lachmann, Linda Feldbrügge, et al.Reagent (1:50; Miltenyi Biotec, 130059–901) and Zombie Aqua™ Fixable Viability Kit (1:250; Biolegend #423101) for 10 min at room temperature. Thereafter, cells were stained with HLA-DR-APC (1:100; Biolegend, 307610)

(7) BPTF is essential for vaccine-induced germinal center B cell responsesJ ImmunolJune 20, 2026Alexandria J Sturtz, Birk K Evavold, Zarifeh Heidari Rarani, Wafaa B Alsoussi, Julian Q Zhou, Hanover C Matz, Hiromi Muramatsu, Wren Simkins, Sameer Kumar Malladi, Katherine M McIntire, et al.Zombie NIR Fixable Viability Kit (BioLegend; Cat#423106) and Zombie Aqua Fixable Viability Kit (BioLegend; Cat#423102). Cells were suspended in the master mix and incubated on ice for 30 min. After two P2 washes, cells

(8) Alveolar Macrophages Undergo Postnatal Transition from a Hyperphagocytic and Proinflammatory Profile to a Tolerogenic ProfileiScienceJune 19, 2026Hang Yu, Shengwen Shang, Qing Xu, Jiabing Tong, Jian Wang, Fengqi Lianti-mouse Ly6G,BioLegend,Cat# 127622; RRID: AB_10643269 Zombie Aqua™ Fixable Viability Kit,BioLegend,Cat#423102; N/A ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(9) Targeting ADAR1 p150 triggers tumor inhibition and antitumor immunity to overcome immunotherapy resistanceiScienceJune 19, 2026Zhichao(Eric) Ai, Yuying Bian, Gang Li, Xiaobin S. Wang, Sining Zhu, Yumeng Li, Xiangyu Yao, Zhipeng Yangradient. Cells were stained with a live/dead viability dye (Zombie Aqua™ Fixable Viability Kit, BioLegend, 423102) followed by surface antigen staining. For intracellular transcription factor and cytokine staining,

(10) Protein-Dependent CD4+ T Cell Specialization and CD8+ T Cell–Driven IL-2 Production in HIViScienceJune 19, 2026Jernej Pušnik, Falko M. Heinemann, Andreas Heinold, Enrico Richter, Stefan Esser, Hendrik StreeckStimulated cells were then washed with PBS and subsequently stained for viability (ZombieAqua, Biolegend, 423102) for 15 min at 4°C. Next, cells were washed with FACS buffer (PBS supplemented with 2% FCS, 0.05% NaN3,

(11) Nicotinamide mononucleotide enhances anti-tumor effect by resetting macrophages toward the inflammatory M1-like phenotypeMol Ther OncolJune 18, 2026Haoran Xu, Marcus Chun Tao Wan, Chelsey Chi Ching Wong, Siqi Qin, Yang Wen, Jun Wang, Zhiwei ChenPacific Blue anti-mouse CD4 (Clone: RM 4–5, Cat# 48-0042-82), Zombie Aqua Fixable Viability Kit (BioLegend, Cat# 423102). For tetramer staining assay, PE-conjugated H-2Kd HIV-1 Gag AMQMLKETI (AI9) MHC class I tetramer (MBL)

(12) Fungal metabolite-based immunotherapy overcomes tumor-associated macrophage immunosuppressionCell Reports MedicineJune 16, 2026Jing Hu, Xiaoman Ju, Biping Deng, Kaicheng Tang, Dongchen Yuan, Xiaofan Sun, Lingmei Kong, Yixin Xu, Zhuo Fu, Stephane Koda, et al.2286861 CSFE,Beyotime,Cat #C0051 7AAD (Clone N/A),BioLegend,Cat # 420404 Zombie Aqua™ (Clone N/A),BioLegend,Cat # 423102 123 count eBeads ebioscience,Thermo Fisher,Cat # 01-1234-42 Human M-CFS protein,Abbkine,Cat # PRP1937 Human

(13) When Protection Turns Pathogenic: Dual Compartment Functions of Myeloid YB-1 in Renal IRIInt J Mol SciJune 10, 2026Anna Leitz, Yili Chen, Xiyang Liu, Yingying Gao, Jialin Wang, Ina Verena Martin, Rafaela Rawinski, Rafael Kramann, Tammo Ostendorf, Ute Raffetsederprofiles, singlet discrimination, and exclusion of dead cells using Zombie Aqua™ viability dye (Biolegend; 423101, San Diego, CA, USA). In the gated populations CD16 MFI was calculated. 4.7. Statistical Analyses Data

(14) ROS-Triggered Self-Aggregation of a β-Elemene Olefin-Rich Nanoemulsion for Mitochondrial-Targeted Metabolic Reprogramming and Colitis Inflammation AlleviationInt J NanomedicineJune 8, 2026Zhiyuan Luo, Luyu Jia, Yu Tang, Yifan Huang, Zhanni Ma, Youpeng Li, Xiaojing Zhang, Pengfei Zhang, Tian Xiefollowing reagents and antibodies from BioLegend (USA) were used: Zombie Aqua™ Fixable Viability Kit (423101), and TruStain FcX™ anti‑mouse CD16/32 (Clone 93, Cat#101319). Fluorochrome‑conjugated antibodies included:

(15) Panaxynol mitigates chemotherapy-induced intestinal mucositis by improving the colonic microenvironment in murine modelsAmerican Journal of Physiology-Gastrointestinal and Liver PhysiologyJune 1, 2026Brooke M. Bullard, Brandon N. VanderVeen, Thomas D. Cardaci, Sierra J. McDonald, Arianna V. Bastian, Nathaniel B. Willis, Mingming Xu, Jie Li, Joseph F. Pierre, Lorne J. Hofseth, et al.Cat#149052), F4/80: naïve macrophages (BioLegend; Cat#123114), Zombie Aqua: live/dead (ZA; BioLegend; Cat#423102), NK1.1: NK-cells (BD Biosciences; Cat#741233), B220: B-cells (BD Biosciences; Cat#612972), CD31: endothelial cells

(16) WDR82 suppresses breast cancer progression by inhibiting ERK-driven chemokine expression and neutrophil infiltrationInt J Biol SciMay 29, 2026Qinyi Yu, Jiang Yang, Fengjiao Lu, Wenxiu Liu, Zeyao Han, Yuanchao Bao, Xiaorui Xu, Jialei Xu, Wanfeng Gao, Boyi Cong, et al.using FACS (BD, LSRFortessaTM X-20 or Sony, MA900). The antibodies included Zombie-Aqua (BioLegend, 423102), APC/Cyanine7 anti-CD45 (BioLegend, 157617), PerCP/Cyanine5.5 anti-CD3 (BioLegend, 100218), Brilliant

(17) Self-Dimerization Induced Proximity Targeting Chimeras (SDIPTAC) Lead to DCAF1 Loss of Function and Inhibition of HIV ReplicationJournal of Medicinal ChemistryMay 28, 2026Mark F. Mabanglo, Smriti Srivastava, Yusuke Matsui, Zichong Li, Mahmoud Noureldin, Justin P. Pogmore, Laurent Hoffer, Fatemeh Taherian, Taraneh Hajian, Sarah Tucker, et al.After 4 days, cells were either unstained (Figure , Panel 1) or stained with Zombie Aqua (BioLegend 423101, for Figure , Panels 2 and 3) and analyzed by FACS for viability (FSC/SSC gating for Figure , Panel1,

(18) Protocol for assessing anti-Siglec-9 antibody-mediated tumor phagocytosis by macrophages using pH-sensitive dyes and flow cytometrySTAR ProtocMay 20, 2026Saitong Muneekaew, Thunchanok Nuchphongsai, Pasut Sasithong, Kritayaporn Saiprayong, Kamonphan Rungrueang, Methichit Wattanapanitchdilution),BioLegend,Cat. No. 351502 Zombie Aqua™ Fixable viability dye (1:500 dilution),Biolegend,Cat. No. 423102 Human TruStain FcX (TM) (1:20 dilution),BioLegend,Cat. No. 422302 True-Stain Monocyte Blocker (1:20

(19) TMPRSS2-induced Golgi disruption restricts the incorporation of virus envelope glycoproteins into virionsEMBO ReportsMay 19, 2026Sayuri Seki, Shigeyoshi Harada, Akiko Sugimoto-Ishige, Midori Unno, Machie Sakuma, Shinsuke Ito, Hiroyuki Yamamoto, Aki Tanabe, Saori Matsuoka, Chieko Makino-Okamura, et al.LTX with Plus reagent,Thermo Fisher Scientific,15338030 Zombie Aqua Fixable Viability Kit,BioLegend,423101 BD Cytofix/Cytoperm,BD Biosciences,554723 BD Perm/Wash,BD Biosciences,554723 Mojo Sort buffer,BioLegend,480017 Sucrose,Sigma,S0389-500G RIPA

(20) Interleukin-4-producing type 2 innate lymphoid cells in the lymph node promote proallergic Tfh13 cell differentiationImmunityMay 19, 2026DongUk Lee, Meng-Ping Lu, Priya Rajamanimuthu, Mrinmoy Das, Enxhi Ferraj, Courtney Fisher, Peri Matatia, Chun-Wei Chen, Raif S. Geha, Caroline L. Sokol, et al.Buffer Set,Thermo Fisher Scientific,Cat# 00-5523-00 Zombie Aqua™ Fixable Viability Kit,BioLegend,Cat# 423102 EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit,STEMCELL Technologies,Cat# 19860 EasySep™

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(1)

1/22/2024Reliable Live Dead Dye From BioLegend with Cell Fixation laura denneyI used Zombie Viability dyes to examine dead cell frequency in flow cytometry samples. Samples needed to be fixed with formaldehyde so I used a live/dead dye which allows fixation.
(1)

1/1/2024BioLegend's Fixable Kit for the Distinction of Live and Dead Cells amirah al jawaznehI used the Zombie Aqua Fixable Viability Kit in both human and mouse cells. The product works great and it is available in different fluorochromes (to be adapted to every FACS panel). The staining ... Read Review
(1)

11/10/2020Good Fixable Viability Dye Anonymous ReviewIf you need to fix your cells, this fixable dye allows you to stain them for viability first and then fix them. Here I was checking the percentage of Tregs in my cell population so I needed to fix/... Read Review
(1)

4/20/2020Blue Zombies Gregor WerbaEasy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.
(1)

4/4/2018Clear Identification Of Dead Cells Chris CorkumOur lab routinely utilizes flow cytometry to investigate our research questions. Eliminating dead cells from flow cytometric data is necessary to obtain quality results, especially when working with ... Read Review

Zombie Aqua™ Fixable Viability Kit from BioLegend

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Zombie Aqua™ Fixable Viability Kit from BioLegend

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