Zombie Aqua™ Fixable Viability Kit 423101 from BioLegend

Product Specs
ItemZombie Aqua™ Fixable Viability Kit
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number423101
Quantity100 tests
ApplicationsFC - Quality tested, ICFC, ICC - Verified

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A commensal protozoan exacerbates acetaminophen-induced liver injury by producing free sphingosineGut MicrobesDecember 31, 2026Xiaoqing Yan, Yusi Shen, Qian Sun, Xiuwen Zhu, Qiuchong Chen, Longxiang Liao, Yuan Zhou, Wanpeng Cheng, Zhen Shi, Zhuanzhuan Liu, et al.with APAP plus sphingosine or TMAO for 24 h, and stained using the Zombie Aqua™ Fixable Viability Kit (423101, Biolegend). For western blot assay, cells were treated with APAP plus sphingosine or TMAO for 12 h,

(2) Fusobacterium nucleatum-derived succinic acid aggravates colitis by triggering macrophage pro-inflammatory phenotypic transformation via SUCNR1/NF-κB axisGut MicrobesDecember 31, 2026Suqi Zeng, Shanshan Jiang, Jianxuan Sun, Yan Chen, Meiqi Qiu, Jiao Li, Qingzhi Lan, Yu Pu, Zongbiao Tan, Xingzhou Guo, et al.were stained according to the respective antibody manufacturers’ protocols. Zombie Aqua viability dye (423102, BioLegend, USA) was used to discriminate live and dead cells. For BMDM, the same staining protocol

(3) DNA prime and peptide boost immunization elicits robust neoantigen-specific CD8 + T cell responses and therapeutic protection in mouse tumor modelsOncoImmunologyDecember 31, 2026Pablo Morgado-Cáceres, Francisca Hofmann-Vega, Diego Figueroa, Juan Saavedra-Almarza, Felipe Gálvez-Cancino, Ximena Díaz, Evelyn Menares, Eduardo Roa, Sofia Hidalgo, Manuel Varas-Godoy, et al.in PBS-FBS 2% by 10 min at 4 °C, washed twice with PBS, incubated with Zombie AquaTM (Biolegend, ref 423102) viability dye diluted in PBS by 20 min at 4 °C, washed twice with PBS, incubated with the antibodies

(4) A NANOBODY molecule that blocks MerTK ectodomain cleavage in vitro and in vivoMAbsDecember 31, 2026Linde Duprez, Ayse Kilic, Hélène Bruwiere, Karim Fekir, Dennis Vlaeminck, Camille De Craene, Daan Geiregat, Kirsten Paesen, Lien Leutenez, Stijn Lambrecht, et al.supplemented with 30 µM HSA. Cells were washed and stained with Zombie Aqua Viability Dye (Biolegend, cat. no. 423101/423102), human Fc-block and mouse anti-FLAG (Sigma-Aldrich, cat. no. F1804) or ABH0074 and anti-mouse

(5) Bone targeted microenvironment actuated engineered exosomes for precision therapy of IBD associated bone lossBioactive MaterialsOctober 1, 2026Jiang Liu, Cong Wang, Taojin Feng, Siyu Chen, Siliang Ge, Yue Zhao, Ming Chen, Zhongqi Wang, Weier Bao, Pincong Fu, et al.in the presence of brefeldin A. The cells were then stained with Zombie Aqua Fixable Viability Kit (423102, BioLegend) to exclude dead cells and with FITC anti-mouse CD4 (100406, BioLegend) antibody for surface

(6) Targeting the αvβ5 Integrin Modifies the TGF-β-Rich Tumor Microenvironment of Pancreatic CancerCancer ResAugust 4, 2026Kodai Suzuki, Yuki Kunisada, Yukihito Kuroda, Hotaka Kawai, Tatiana Hurtado de Mendoza, Evangeline S. Mose, Shingo Eikawa, Henri Havia, Tero A. H. Järvinen, Caisheng Lu, et al.(clone RMV-7; Cat# 740755, RRID: AB_2870648; BD Biosciences), or Aqua™ Fixable Viability Kit (Cat# 423101; BioLegend). Intracellular staining of immune cells was performed using the following Abs and a Foxp3

(7) Targeting TOMM40 unleashes immunogenic mitophagy to facilitate antigen presentation and immunotherapy sensitizationJ Immunother CancerJuly 27, 2026Zhiwei Zhang, Yu-An Xie, Chenglin Mu, Shiying Sheng, Jinyuan Zhang, Jie Han, Jiwen Li, Zhengfu He, Xia Liustaining. For viability assessment, cells were stained with a viability dye (Zombie Aqua, BioLegend, Cat# 423101) for 10–15 min at room temperature to exclude dead cells from the analysis. After washing with PBS,

(8) Synergistic nanoparticle dyad improving tumor immunogenicity and reshaping tolerogenic dendritic cells in hepatocellular carcinomaMater Today BioJuly 23, 2026Chang Liu, Xiejun Zhao, Hui Song, Yiwen Yuan, Jia Ma, Yingtong Dong, Tianzhao Xu, Xiaojiao Li, Xinghui Liusorter, and analyzed by the FlowJo software (Tree Star). Zombie Aqua™ Fixable Viability Kit (BioLegend, 423101), anti-CD45-APC/Cy7 (eBioscience, 47045182), anti-mouse-CD45-FITC (BioLegend, 103108), anti-CD4-PE (Biolegend,

(9) Increased Expression of HLA-DR and CD69 on Peripheral CD4+ T Cells Predicts Better Clinical Outcomes in Cutaneous MelanomaImmunotargets TherJuly 22, 2026Ana Tomás, José Maximino, Hugo Nunes, Rute Salvador, Rafael Luís, Cheila Brito, Diana P Saraiva, Emanuel Gouveia, Carolina Pereira, Filipe Gonçalves, et al.× 106 cells) or tumor suspensions were stained with Zombie Aqua™ Fixable Viability Dye (BioLegend, 423102; 1:200) in PBS 1× for 30 min, washed in PBS with 2% FBS and 1 mM EDTA, and incubated with surface antibodies

(10) Endothelial Senescence-Associated Secretory Signaling Promotes Macrophage Extracellular Traps Formation and Contributes to the Exacerbation of Combined Lung InjuryInt J Biol SciJuly 20, 2026Wentao Xie, Hongsen Liao, Yali Dai, Lin Chen, Yujie Li, Zhuo Cheng, Chunmeng ShiFc receptor blocking reagent (Biolegend, 156604), stained with Zombie Aqua live/dead dye (Biolegend, 423101), and then stained for surface markers. For intracellular targets, cells were fixed and permeabilized

(11) Whole blood exchange-lymphoplasmapheresis combined transfusion as an immunotherapy in systemic lupus erythematosusiScienceJuly 17, 2026Zhimin Zhang, Junpeng Zhao, Siya Pei, Wenyu Yin, Bijuan Li, Ying Jiang, Ning Liat 37°C for 6–8 h (eBioscience, Cat# 00–4975). Zombie Aquat Fixable Viability Kit (BioLegend, CAT # 423101) was used to exclude the dead cells. Single-cell suspensions were blocked with Human TruStain-FcXTM

(12) Naive CD4+ T cells synthesize a hyaluronan-rich glycocalyx that is diminished following activationiScienceJuly 17, 2026Laxmi Prasad Adhikari, Jessica Contreras, Kristen M. Patterson, Meghan M. Moran, Erin N. Howe, Anna H.K. Plaas, Joseph M. Reynolds80190 Pam3CSK4,InvivoGen,Cat#: tlrl-pms Stains-All,Sigma-Aldrich,Cat#: E9379 Zombie-Aqua Fixable Viability Kit,Biolegend,Cat#: 423102 "Pierce 16% Formaldehyde (w/v), Methanol-free",Thermo scientific,Cat#: 28908 TRIzol Reagent,Thermo Fisher Scientific

(13) Fluorescence tracking CD8 T cells from tumor-draining lymph nodes to irradiated tumors and their activation in the tumor environmentJ ImmunolJuly 17, 2026David Friedman, Gwen Kramer, Alejandro F Alice, Jason Baird, Sina Ramin, Terry Medler, Kristina H Young, Madeleine G Kaiser, Clemens Grassberger, Marka R Crittenden, et al.as previously described,9 2 × 106 cells were stained with Zombie Aqua Viability Dye from BioLegend (#423102) in PBS for 10 min on ice, then Fc receptors were blocked with α-CD16/CD32 antibodies from BD Biosciences

(14) Identification, characterization, and expansion of innate lymphoid cells derived from canine peripheral blood mononuclear cellsFront ImmunolJuly 15, 2026Yeganeh Mehrani, Lily Chan, Charly McKenna, Mikaela Eng-Toogood, Fernando de Paula Freitas, Emma Vanderboon, J. Paul Woods, Byram W. Bridle, Khalil Karimipolyclonal antibody to prevent nonspecific binding, followed by incubation with a Live/Dead Fixable Dye (423101/423102, BioLegend) to exclude dead cells. Surface marker labeling was performed by incubating PBMCs

(15) IL7R depletion mitigates neuroinflammation and ischemic stroke via AKT dephosphorylation dependent LCP1 suppression in miceNeurotherapeuticsJuly 14, 2026Qianqian Yin, Shaomeng Chen, Decao Yang, Ruoxi Li, Hang Hang, Chan Xu, Xiaoyue Wang, Jinxin Li, Jingyi Zhao, Jianing Wang, et al.101301, Biolegend, USA). After 10 min of incubation, we used Zombie Aqua™ Fixable Viability Kit (Cat# 423101, Biolegend, USA) for live and dead staining. After 10 min of incubation, we performed cell marker staining

(16) Harnessing human immune system models to validate NADPH oxidase 1 inhibition as treatment for hepatocellular carcinomaFront PharmacolJuly 14, 2026Zenzi De Vos, Lander Heyerick, Aline Baekelandt, Lara Morren, Anne Hoorens, Hasan Eker, Luís Abreu de Carvalho, Filip Gryspeerdt, Frederik Berrevoet, Hannah Stocks, et al.blood and 1/15 for tissue. All antibodies, FcR blocks (mouse #156604, human #422302), live/dead stain (#423102) and counting beads (#424902) were purchased at Biolegend. Samples were measured on the BD FACSSymphony

(17) Bcl11b dose-dependently regulates positive selection of CD8 T cells to the virtual memory fatebioRxivJuly 9, 2026Tom Sidwell, Ellen V RothenbergCell viability was marked using fixable viability dyes (Thermo Fisher L34990 and 65–0866-14, Biolegend 423101) stained in PBS for 15 minutes on ice protected from light, or with propidium iodide (Sigma P4170) or

(18) Phenylalanine exacerbates psoriasiform inflammation through NF‑κB‑mediated dendritic cell activation and Th17 polarizationInt J Mol MedJuly 8, 2026Yaohan Xu, Jing Pan, Jie Chen, Yutao Zhu, Miaolian Cai, Zeyu Ma, Siji Chen, Tianze Yu, Yushu Wei, Jingying Pan, et al.404-9668-80; eBioscience; Thermo Fisher Scientific, Inc.). The Zombie Aqua™ Fixable Viability Kit (cat. no. 423101; BioLegend, Inc.) was also used. After washing, the cells were assayed with a CytoFLEX LX flow cytometer

(19) Regulatory T cells sabotage anti-tumor γδ T cells by creating IL-2-deficient environmentsJ Exp MedJuly 6, 2026Rafael Blanco-Domínguez, André Miguel Vaz-Pinto, Leandro Barros, Noella Lopes, Beatriz Henriques-Alves, Mariana Carreira, Carlos Labão-Almeida, Julie C. Ribot, Gonçalo J.L. Bernardes, Bruno Silva-Santos, et al.,BD Biosciences,562077 ,Viability,Zombie Yellow,500,,BioLegend,423103 ,Viability,Zombie Aqua,500,,BioLegend,423101 List of antibodies and dyes used for flow cytometry analyses

(20) CD38 defines a therapeutically targetable pathogenic T cell population for precision immunotherapy in autoimmune diabetesbioRxivJuly 3, 2026Shiva Pathak, Rizwan Ahmed, Nadine Nagy, Sooyeon Lee, Cameron S. Bader, Shobha Regmi, Bettina P. Iliopoulou, Pin-I Chen, Biki Gupta, Alejandro Villar-Prados, et al.transcriptomic analysis. Flow cytometry Live cells were identified by Live/Dead Aqua (BioLegend; Cat. 423102) and stained with antibodies at a final dilution of 1:200. The following antibodies were used for flow cytometry

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(1)

1/22/2024Reliable Live Dead Dye From BioLegend with Cell Fixation laura denneyI used Zombie Viability dyes to examine dead cell frequency in flow cytometry samples. Samples needed to be fixed with formaldehyde so I used a live/dead dye which allows fixation.
(1)

1/1/2024BioLegend's Fixable Kit for the Distinction of Live and Dead Cells amirah al jawaznehI used the Zombie Aqua Fixable Viability Kit in both human and mouse cells. The product works great and it is available in different fluorochromes (to be adapted to every FACS panel). The staining ... Read Review
(1)

11/10/2020Good Fixable Viability Dye Anonymous ReviewIf you need to fix your cells, this fixable dye allows you to stain them for viability first and then fix them. Here I was checking the percentage of Tregs in my cell population so I needed to fix/... Read Review
(1)

4/20/2020Blue Zombies Gregor WerbaEasy to use viability dye with emission spectrum that integrates nicely with Brilliant Violet dyes.
(1)

4/4/2018Clear Identification Of Dead Cells Chris CorkumOur lab routinely utilizes flow cytometry to investigate our research questions. Eliminating dead cells from flow cytometric data is necessary to obtain quality results, especially when working with ... Read Review

Zombie Aqua™ Fixable Viability Kit from BioLegend

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Zombie Aqua™ Fixable Viability Kit from BioLegend

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