Q5 Site-Directed Mutagenesis Kit E0554S from New England Biolabs

Product Specs
ItemQ5 Site-Directed Mutagenesis Kit
CompanyNew England Biolabs
Price $215.00 Inquire
Catalog NumberE0554S
Quantity10 reactions
ApplicationsDNA Manipulation
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) PARP4 ADP-ribosylates PIDD1 to complete a phospho/SUMO/PAR-ylation cascade that orchestrates PIDDosome assemblySci AdvApril 29, 2026Richa B. Shah, Yuanyuan Li, Ashley Person, Léonie Frigon, Nina Ðukić, Ela Kini, Raymond Chen, John M. Pascal, Michael S. Cohen, Ivan Ahel, et al.VSV-RAIDD-FL according to the manufacturer’s instructions using a Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). GFP-tagged PARP4, yellow fluorescent protein (YFP)–tagged PARP14 WT, G832E, and R1699A cDNAs were

(2) Piezo1-mediated mechanohydraulic control of cell volume drives cardiac morphogenesisSci AdvApril 24, 2026Christina Vagena-Pantoula, Konstantinos Kalyviotis, Shuyi Feng, Antoine Sanchez, Igor Kondrychyn, Moe Fukumoto, Xiangbin Pan, Thomas Juan, Didier Y. R. Stainier, Periklis Pantazis, et al.KK5101). Mutagenesis Site-directed mutagenesis was performed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, NEB, E0554S) according to the manufacturer’s guidelines. Primers for site-directed mutagenesis were designed using

(3) Polarized SOS activity orchestrates FGF-directed cell migration in vivoCurrent BiologyApril 20, 2026Theresa V. Gibney, Laila Y. Latifi, Jacob I. Mardick, Neal R. Rasmussen, Maria C. Lyons, Meera V. Sundaram, David J. Reiner, Ariel M. PaniQ5 High-Fidelity 2X Master Mix,New England Biolabs,M0492L Q5 Site-Directed Mutagenesis Kit,New England Biolabs,E0554S Key Resources Table

(4) SPNS2 exports sphingosine-1-phosphate and imports glucoseNat CommunApril 20, 2026Cynthia Weigel, Md Lokman Hossen, Ryan D. R. Brown, Can E. Senkal, Christopher D. Green, Jason Newton, Sumit Saha, Elisa N. D. Palladino, Bin Ni, Francesco S. Celi, et al.e.g. substitution of single nucleotides, was performed with the Q5 Site-Directed Mutagenesis Kit (#E0554S, New England Biolabs) and double-stranded SPNS2 (NM_001124758) Human Tagged ORF Clone in pCMV6-AC-GFP.

(5) hnRNPUL1 has a dead polynucleotide kinase domain that regulates RNA and protein interactionsiScienceApril 17, 2026Carmen V. Apostol, Ang Li, Peter Daniels, Llywelyn Griffith, Johnathan Cooper-Knock, Elisa Aguilar-Martinez, Ivaylo D. Yonchev, Ashleigh G.R. Whelan, Pamela J. Shaw, Ian M. Sudbery, et al.Gibson assembly cloning kit,New England Biolabs,Cat# E5510S Q5 Site-Directed Mutagenesis Kit,New England Biolabs,Cat# E0554S ,, Deposited data,Deposited data,Deposited data ,, Uncropped gel images,This paper,https://doi.org/10.17632/rh3prrcskn.1 ,, Experimental models

(6) Chromatin association promotes UBR5-mediated degradation of RbbioRxivApril 17, 2026Shuyuan Zhang, Michael C Lanz, Joshua Konschnik, Jan M Skotheimvariants in cells, we used a doxycycline-inducible Rb cassette35, and performed site-directed mutagenesis (E0554S, NEB) to generate Rb variant plasmids. All the plasmids were PiggyBac transposon vectors containing

(7) AMPK/mTORC2/AKT-473/RUNX2 signaling axis modulates epithelial-mesenchymal transition and bone tropism in breast cancerFront OncolApril 10, 2026Meher Bolisetti Gayatri, Abhayananda Behera, Suresh Chava, Preeti Vyakaranam, Ganesh Venkatraman, Suresh Kumar Rayala, Aramati Bindu Madhava Reddyvector followed by S118A and S118D site-directed mutation using the NEB site-directed mutagenesis kit (#E0554S). Nuclear and cytosolic extraction Cells were treated with the indicated drugs for 6 hours then collected

(8) c-MYC is an aggregation-prone, amyloidogenic proteinbioRxivApril 9, 2026Ling Lin, Kun-Han Chuang, Chengkai Dai2xHA-MYCΔC+ΔP12, and 2xHA-MYCΔC+ΔP2+ΔP12 were generated using the Q5 Site-Directed Mutagenesis kit (cat#E0554S, New England Biolabs Inc.). pMYC-SEAP was purchased from Clontech Laboratories (Cell signaling pathway

(9) PHIP suppresses NuRD to enable the growth of SWI/SNF-mutant cancersNat CommunApril 7, 2026Hayden A. Malone, Jacquelyn A. Myers, Emma G. Gruss, Marc A. Morgan, Jake D. Friske, Tabitha C. McCarty, John J. Navarro, Sarah Robinson, Rebecca L. Halliburton, Sandra J. Kietlinska, et al.deleted from the Xlone-PHIP construct by using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs E0554S). Cells received the Super PiggyBac Transposase expression vector (System Biosciences, PB210PA-1) and

(10) Heparan sulfate is essential for Drosophila FGF exportbioRxivApril 6, 2026Guilherme Oliveira Barbosa, Christian Solis-Calero, Thomas B. KornbergThe mutations were made on pAc5.1-Bnl:GFP11(7x) with the Q5® Site-Directed Mutagenesis Kit (NEB Cat#: E0554S). A set of four plasmids were created to track the biosynthetic pathway of Bnl by GFP reconstitution.

(11) G protein Gαq subunits engage targets in the nucleus involved in chromatin remodeling and gene expressionJournal of Biological ChemistryApril 1, 2026Joseph Loomis, Naincy Chandan, Michael Burroughs, Saji Abraham, Rongxi Zhang, Yiyi Cao, Matthew J. Brody, Gregory G. Tall, Alan V. SmrckaC-terminal V5-tag in these constructs using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). This resulted in POI-V5-NP or POI-V5-SmB constructs in pLenti6.3-V5/DEST, such as SMARCD3-V5-NP. Plasmid

(12) ZXDB Drives Macrophage Inflammatory Programming in Sepsis-Induced Acute Kidney Injury by Recruiting EIF4A3 to Enhance ACACA TranslationFASEB JMarch 31, 2026Haiyang Wu, Wei Gang, Li Wang, Jing Sun, Shuangxi Li, Zhiyong Guodeleted aa 151–300) was made using the site‐directed mutagenesis kit (Q5 Site‐Directed Mutagenesis Kit, E0554S; NEB). Plasmids were transferred into cells using Lipofectamine 3000 (L3000015; Invitrogen) following

(13) NLGN3 autism variants have distinct functional impact on synapses and sleep behavior in DrosophilabioRxivMarch 30, 2026Rebekah E. Townsley, Jonathan C. Andrews, Saurabh Srivastav, Sharayu V. Jangam, Shabab B. Hannan, Oguz Kanca, Shinya Yamamoto, Michael Francis Wanglerp.R597W) were generated using NEBase Changer Q5 site-directed mutagenesis kit (New England Biolabs Inc #E0554S) and verified by Sanger sequencing. Reference and variant coding sequences (CDS) were subcloned into

(14) Microvillus inclusion disease-associated MYO5B deficiency impairs endosome-to-mitochondrion iron transferGastroenterol Rep (Oxf)March 27, 2026Chang Sun, Changsen Leng, Mingyue Sun, Yuan Lin, Mingqian Xu, Águeda Martinez-Barriocanal, Albert Gerding, Jeroen Kuipers, Jim de Leeuw, Barbara M Bakker, et al.USA). MYO5B variants were generated by using the pENTR1A plasmid. The Q5 Site-Directed Mutagenesis Kit (E0554S; New England Biolabs, Ipswich, MA, USA) with primers designed with the NEBaseChanger tool was used to

(15) Chromatin dynamics of the Klf4 locus in mouse pluripotent cellsScientific ReportsMarch 27, 2026Jente van Staalduinen, Hélène Kabbech, Selçuk Yavuz, Ridvan Cetin, Agnese Loda, Wiggert van Cappellen, Adriaan Houtsmuller, Kerstin Wendt, Ihor Smal, Frank Grosveldbp 5’ of the PAM site) via a Q5 Site-Directed Mutagenesis reaction (New England Biolabs, Catalog no: E0554S). For the Rad23b promoter and second control region, gBlocks Gene Fragments (synthesized by IDT, see

(16) Selective regulation of transsynaptic alignment and postsynaptic assembly by a novel NCAM family synaptic adhesion moleculebioRxivMarch 16, 2026Paola Van der Linden Costello, Marina N. Wennerberg, Jerrik A. Rydbom, Scott Gratz, Lauren F. Fennema, Kate M. O'Connor-Giles, Heather T. Broihier(5’-TTGCTGGCGATGGGGCAACA-3’) into the pU6–3-gRNA plasmid (DGRC 1362) via site-directed mutagenesis (NEB Cat #E0554S). The homology-directed repair and gRNA plasmids were then injected into vasa>Cas9 embryos (BDSC 51324;

(17) Oncogenic SOX9-TRAIP signaling drives gastric cancer progression by mediating the degradation of the CPEB3-mTORC1 tumor suppressor axisWorld Journal of Surgical OncologyMarch 14, 2026Yun Zhou, Jin Zhu, Canhui Ouyang, Wei Liu, Lixing Huang, Lingting Zou, Bin Liu, Zhiqiong Luhuman genomic DNA (Promega) and inserted into the pGL3-Basic vector. The Q5 Site-Directed Mutagenesis Kit (NEB, Cat#E0554S) was employed to introduce a specific point mutation (CCATTGTTC to CCAAAATTC) into the SOX9 binding

(18) The yeast phosphofructokinase β-subunit has RNA unwinding activity and modulates cell cycle progressionNucleic Acids ResMarch 12, 2026Waleed S Albihlal, Ana M Matia-González, Tobias Schmidt, Wieland Mayer, Joe Bryant, Martin Bushell, Dierk Niessing, Matteo Barberis, Alexander Schmidt, Jürgen J Heinisch, et al.(New England Biolabs, M0554S), LR Clonase II plus kit (Thermo Fisher, 12538120), Q5 site-directed Mutagenesis kit (NEB, E0554S), Dynabeads™ mRNA DIRECT™ Purification kit (Thermo Fisher, 61011), Pierce™ bicinchoninic acid (BCA)

(19) microRNA-422a promotes HIV replication and innate immune evasion by targeting MECP2Molecular Therapy Nucleic AcidsMarch 12, 2026Li Du, Jean-Noël Billaud, Sushama Telwatte, Nikhila Kadiyala, Prerna Dabral, Mohamed S. Bouzidi, John Guatelli, Steven A. Yukl, Satish K. Pillaiwithin the two predicted target sites using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). The following primers were used for site mutations: For MECP2 3′UTR SITE1 Mutant: forward 5′-TCAAGTCTAGCGTAGTGCAGCA-3′,

(20) CXCR4-tropic HIV-1 infection in an immunocompetent monkey modelNature CommunicationsMarch 9, 2026Nicholas R. Meyerson, Vanessa L. Bauer, Will T. Fattor, Cody J. Warren, Arturo Barbachano-Guerrero, Melisa J. Weiss, Obaiah Dirasantha, Bridget L. Burris, Adetunji S. Adesina, Joseph L. Timpona, et al.(https://nebasechanger.neb.com) and the standard protocol of the Q5 Site-Directed Mutagenesis Kit (New England Biolabs #E0554S). The sequences of HIVnom1, HIVnom2, and HIVnom3 can be found at GenBank with accession numbers PX925169,

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(1)

1/24/2024Mutagenesis Kit From New England Biolabs Enables Rapid, Site-Specific Mutagenesis of Double-Stranded Plasmid DNA Aditi KothariThe Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase... Read Review
(1)

4/14/2023Great Kit for Site-Specific Mutation YONG MThe Q5® Site-Directed Mutagenesis Kit was purchased to create the point mutation in plasmid. The protocol is very clear and the steps is pretty simple and fast. Site mutation is successfully created.

Q5 Site-Directed Mutagenesis Kit

Q5 Site-Directed Mutagenesis Kit from New England Biolabs

New England Biolabs

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Q5 Site-Directed Mutagenesis Kit from New England Biolabs

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