Q5 Site-Directed Mutagenesis Kit E0554S from New England Biolabs

Product Specs
ItemQ5 Site-Directed Mutagenesis Kit
CompanyNew England Biolabs
Price $215.00 Inquire
Catalog NumberE0554S
Quantity10 reactions
ApplicationsDNA Manipulation
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The mRNA architecture of the translation termination site primes programmed stop codon readthrough events in DrosophilaRNA BiolDecember 31, 2026Laveena Kansara, Georg Wolfstetter, Daniel Wintermayr, Ioannis Alexopoulos, Alejandra Escos, Marc Friedländer, Ylva EngströmHk, yemanuclein, CG10321 and CG6282 genes were made with the Q5®-site directed mutagenesis kit (NEB, #E0554S) according to the manufacturer’s instructions. The Renilla luciferase control plasmid (Addgene #38093)

(2) Human germline biallelic loss-of-function OSMR variants cause severe allergic diseaseJ Hum ImmunJuly 6, 2026Simran Samra, Mehul Sharma, Julia Körholz, Yihui Liu, Alyssa James, Christina Michalski, Pariya Yousefi, Kate L. Del Bel, Henry Y. Lu, Ashish A. Sharma, et al.FPLCA variants (c.2072T>C, c.1891G>T, c.1538G>A, c.1385A>G), a Q5 site-directed mutagenesis kit (Cat#: E0554S; New England Biolabs) was used according to the manufacturer’s recommendations, with primer pairs noted

(3) Genetic code expansion for single noncanonical amino acid incorporation into SARS-CoV-2 Omicron spike on amber-free lentiviral particlesMethods in EnzymologyJune 25, 2026Wang Xu, Narendra Kumar Gonepudi, Yang Han, Maolin LuY1625 Yeast extract,Sigma,Cat # S3014 Agar,Sigma,Cat # A1296 Q5® site-directed mutagenesis kit,NEB,Cat # E0554S ONE-Glo™ luciferase assay system,Promega,Cat # E6120 4x Laemmli sample buffer,Bio-Rad,Cat # 1610747 Experimental Models

(4) Bioorthogonal click-chemistry labeling and visualization of dual noncanonical amino acid-incorporated HIV-1 Env on intact virionsMethods in EnzymologyJune 25, 2026Narendra Kumar Gonepudi, Wang Xu, Harry Baffour Awuah, Maolin LuYeast extract,Sigma,Cat # S3014–1KG Agar,Sigma,Cat # A1296–1KG Q5® Site-Directed Mutagenesis Kit,NEB,Cat # E0554S ONE-Glo™ Luciferase Assay System,Promega,Cat # E6120 6x Laemmli sample buffer,Thermo scientific,Cat

(5) Induction of broadly neutralizing HIV antibodies by a two-step mechanism informs vaccine designScienceJune 18, 2026Ashwin N. Skelly, Harry B. Gristick, Hui Li, Edem Gavor, Andrew J. Connell, Edward F. Kreider, Lorie Marchitto, Michael P. Hogarty, Maddy L. Newby, Joel D. Allen, et al.D140N) in the plasmid encoding this parental strain using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S). Mutants in this and other backbones were generated using the same strategy. Mutagenized plasmids were

(6) TRK-T3 condensate organization drives growth signalingLife Sci AllianceJune 17, 2026Sindy R Chavez, Savannah M Bogus, Samantha N Cheung, Winnie Zhou, Lilja Gudmundsson, Kaho Kato, Andreas M Ernst5′-TTCAAGGGGTCTTGGCTGG-3′ and using the Q5 site-directed mutagenesis kit as per the manufacturer’s instructions (E0554S; NEB). Sequences were confirmed as above. GRB2-EGFP plasmid was obtained from Addgene (Plasmid #86873).

(7) A protein interactome for the last eukaryotic common ancestor illuminates the biochemical basis of modern genetic diseasesCell GenomicsJune 10, 2026Rachael M. Cox, Ophelia Papoulas, Shirlee Shril, Chanjae Lee, Tynan P. Gardner, Zoya T. Ansari, Anna M. Battenhouse, Muyoung Lee, Kevin Drew, Claire D. McWhite, et al.respectively. The pCS10R MCC GFP-EFHC2 R133H construct was generated by site-directed mutagenesis (NEB, #E0554S) from pCS10R MCC GFP-EFHC2. Capped mRNAs were synthesized using the mMESSAGE mMACHINE SP6 transcription kit

(8) Endoplasmic reticulum-resident α-glucosidase II drives non-small cell lung cancer progression via regulation of secretory glycoproteinsJCI InsightJune 8, 2026Shike Wang, Na Ding, Angelo Chen, Derrick Cardin, Yuting Xu, Kate Grimley, William K. Russell, Jun Xu, Jonathan M. Kurie, Guan-Yu Xiao, et al.manufacturer’s instructions for the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, catalog E0554S). Constructs expressing Flag-tagged LH2 and GFP-tagged TIMP1 have been described previously (32). Lentivirus

(9) CONSERVATION AND DIVERGENCE WITHIN THE ARABIDOPSIS TPL/TPR COREPRESSOR FAMILYbioRxivJune 5, 2026Benjamin L.R. Downing, Maria Pattichis, Fabian E. Vaistij, Mohamed Farawila, Edward Ghannam, Linda Nguyen, Katherine Denby, Alexander Leydon, Jennifer Nemhauser23 (TPR2/TPR3), and 4 (TPR4) clades were synthesized using Q5 Site-Directed Mutagenesis (NEB, Cat #E0554S) and introduced into the appropriate AtARCSc backbone by Golden Gate assembly. All variant sequences

(10) Mechanistic design of cell-penetrating disruptors for phospho-dependent TACC3-CHC interactionStructure (London, England : 1993)June 4, 2026Gunning V, Batchelor M, Alexander KK, Walko M, Dill TC, Burgess SG, Royle SJ, Kennedy EJ, Bayliss R.mutations K506A and K507A were introduced by site-directed mutagenesis using Q5 mutagenesis kit (NEB, E0554S). GB1-TACC3 is the small protein GB1 104 fused to a short, disordered peptide sequence from human TACC3

(11) Modulation of Oncogenic KRAS Signaling by Branched Actin-driven Cell Membrane ProtrusionsResearchSquareJune 3, 2026Gaudenz Danuser, Gabriel Gihana, Kushal Bhatt, Bo-Jui Chang, Vasanth Siruvallur Murali, Felix Zhou, Jungsik Noh, Roshan Ravishankar, Hazel Borges, Jinlong Lin, et al.competent cells using the KLD reaction of the Q5 site directed mutagenesis kit (New England Biolabs, E0554S). The plasmid was sequenced for verification. The genes cloned into the pLVX vector were inserted after

(12) Application of Post-Glycosylation Modifying Enzymes for Mass Spectrometry Imaging of Modified N -Glycans In SituJournal of the American Society for Mass SpectrometryJune 3, 2026Julia E. Dreifus, Léa Chuzel, Edwin E. Escobar, Grace Grimsley, Hongxia Bai, Andrew J. Hanneman, Samantha L. Fossa, Vanessa Gregory, Richard J. Martin, Richard R. Drake, et al.Site-directed mutagenesis of BT3109p was performed using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) was used to convert the catalytic serine to a cysteine (S57C), enabling conversion to formyl glycine

(13) CTCF directly binds G-quadruplex structures to regulate genome topology and gene expressionProceedings of the National Academy of SciencesJune 2, 2026Daniela Samaniego-Castruita, Isabella Han, Roxroy C. Morgan, Samantha Carpenter, Bryce Williams, Abhijit Chakraborty, Ishwar Radhakrishnan, Ferhat Ay, Samuel A. Myers, Anjana Rao, et al.CTCF was performed following the Q5 Site-Directed Mutagenesis Kit from New England BioLabs (NEB Cat# E0554S) with modifications. Forward and reverse primers designed for the mutation were synthesized by Integrated

(14) Palmitoylated importin α recruits PKCε to the plasma membrane to drive breast cancer cell motilitybioRxivJune 1, 2026M. Kathryn Malone, Christopher W. BrownleePKCε-HA-ΔNLS mutant. The PKCε-HA-ΔNLS mutant was generated using the Q5 site-directed mutagenesis kit (E0554S, New England Biolabs) with primer sets F: 5’-aaagcgCTCGCTGCTGGTGCTG-3’ and R: 5’-gccgccTTGGCCACTGTTGGTG-3’

(15) SFPQ prevents MDA5-mediated activation of innate immunity and preserves cell viabilityCell ReportsMay 26, 2026Huirong Zhang, Runjie Yuan, Praneet K. Sandhu, Justin T. Landis, Dirk P. Dittmer, Blossom Damaniamutant plasmids were PCR amplified from full-length SFPQ with the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) using the indicated primers (sequences are listed in Table S3). HUVEC-Cas9-expressing cells were transfected

(16) Microtubule-templated actin assembly by septin9 drives apical expansion of epithelial cellsbioRxivMay 23, 2026Angelo Arrigo, Victoria Hua, Elias T. Spiliotis, Saurabh Kulkarni,New England Biolabs,R0189S KpnI-HF,New England Biolabs,R3142S Q5 Site-Directed Mutagenesis Kit,New England Biolabs,E0554S mMESSAGE mMACHINE™ SP6 Transcription Kit,Invitrogen,AM1340 Purification Kits,Purification Kits,Purification

(17) Interactomics of SARS-CoV-2 Macrodomain 1 Reveals Putative Clients of ADP-Ribosyl Hydrolase ActivityVirusesMay 22, 2026Crissey D. Cameron, Grace Heilmann, Brynn K. Roman, Lars PlateTable 1 and Q5 site-directed mutagenesis according to manufacturer protocols (NEB, Ipswich, MA, USA, E0554S). Site-directed mutagenesis was then conducted using PFU Ultra II Fusion HS DNA polymerase (Agilent,

(18) Novel bispecific T-cell engagers overcoming acquired EGFR resistanceMAbsMay 19, 2026Lennart Kühl, Ann-Kathrin Löffler, Oliver Seifert, Dennis Michler, Miriam Kuhlmann, Giulio Russo, Philipp Kuhn, Helena Nowack, André Frenzel, Thomas Schirrmann, et al.Mutations in the ECD of human EGFR were introduced by site-directed mutagenesis (New England Biolabs #E0554S). Recombinant protein was isolated from supernatants using protein A resin (Fc-comprising molecules;

(19) Epistasis and background dependence in the evolution of Omicron variants of the SARS-CoV-2 spike proteinMol Biol EvolMay 16, 2026Alief Moulana, Thomas Dupic, Michael M Desaiselection and a yeast mating factor for protein localization. Using site-directed mutagenesis (NEB #E0554S), we generated additional plasmids expressing RBD variants Wuhan Hu-1 + Q498R (pAB102), Wuhan Hu-1 +

(20) Drug-induced changes in connectivity to midbrain dopamine cells revealed by rabies monosynaptic tracingElifeMay 15, 2026Katrina Bartas, Pieter Derdeyn, Guilian Tian, Jose J Vasquez, Ghalia Azouz, Cindy M Yamamoto, May Hui, Kevin T BeierRRID:Addgene_232922", Commercial assay or kit,Q5 Site-directed mutagenesis kit,New England Biolabs,E0554S, Commercial assay or kit,Luna Universal One-Step RT-qPCR Kit,New England Biolabs,E3005L, "Chemical

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(1)

1/24/2024Mutagenesis Kit From New England Biolabs Enables Rapid, Site-Specific Mutagenesis of Double-Stranded Plasmid DNA Aditi KothariThe Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase... Read Review
(1)

4/14/2023Great Kit for Site-Specific Mutation YONG MThe Q5® Site-Directed Mutagenesis Kit was purchased to create the point mutation in plasmid. The protocol is very clear and the steps is pretty simple and fast. Site mutation is successfully created.

Q5 Site-Directed Mutagenesis Kit

Q5 Site-Directed Mutagenesis Kit from New England Biolabs

New England Biolabs

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Q5 Site-Directed Mutagenesis Kit from New England Biolabs

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