NEBNext Ultra II Directional RNA Second Strand Synthesis Module E7550L from New England Biolabs

Product Specs
ItemNEBNext Ultra II Directional RNA Second Strand Synthesis Module
CompanyNew England Biolabs
Price $1,270.00 Inquire
Catalog NumberE7550L
Quantity96 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Transcription elongation can be sufficient, but is not necessary, to advance replication timingEMBO ReportsMarch 24, 2026Athanasios E Vouzas, Takayo Sasaki, Juan Carlos Rivera-Mulia, Jesse L Turner, Amber N Brown, Karen E Alexander, Laura Brueckner, Bas van Steensel, David M GilbertSynthesis Module (NEB, E7771) and the NEBNext Ultra II Directional RNA Second Strand Synthesis Module (NEB, E7550). Libraries were prepared from cDNA using the NEBNext Ultra II Library Prep kit (NEB, E7645), indexed

(2) R-loop editing by DNA cytosine deaminase APOBEC3B modulates the activity of oestrogen receptor enhancersNature CommunicationsFebruary 18, 2026Chi Zhang, Yu-jing Lu, Bingjie Chen, Zhiyan Bai, Qiaoxi Zeng, Alexia Hervieu, Marco P. Licciardello, Konstantinos Mitsopoulos, Bissan Al-Lazikani, Marcello Tortorici, et al.material of the directional second-strand synthesis method following the manufacturer’s instructions (NEB E7550). The resultant DNA was purified using SPRI beads (Thermo Fisher) at a volume ratio of 2.0 and eluted

(3) Overcoming host restrictions to enable continuous passaging of GII.3 human norovirus in human intestinal enteroidsSci AdvFebruary 6, 2026Gurpreet Kaur, Sue E. Crawford, Sara Javornik Cregeen, Anil Surathu, B. Vijayalakshmi Ayyar, Carmen V. Apostol, Hoa Nguyen Phuc, Khalil Ettayebi, Aaya Boussattach, Xi-Lei Zeng, et al.(E7525L; New England Biolabs Inc.) and NEB Next Ultra II Directional RNA Second Strand Synthesis Module (E7550L; New England Biolabs Inc.). The double-strand cDNA was purified using 1.8× volume of AMPure XP beads

(4) Jpx RNA controls Xist induction through spatial reorganization of the mouse X-inactivation centerDevelopmental CellNovember 3, 2025Hyun Jung Oh, Priyojit Das, Roy Blum, Andrea J. Kriz, Hun-Goo Lee, Yong-Woo Lee, Jeannie T. Leesecond-strand synthesis using NEBNext Ultra II Directional RNA Second Strand Synthesis Module (NEB, E7550S) containing a dUTP mix. Libraries were generated according to the manufacturer’s instructions using

(5) Master transcription-factor binding sites constitute the core of early replication control elementsEMBO JJuly 17, 2025Jesse L Turner, Laura Hinojosa-Gonzalez, Takayo Sasaki, Satoshi Uchino, Athanasios Vouzas, Mariella S Soto, Abhijit Chakraborty, Karen E Alexander, Cheryl A Fitch, Amber N Brown, et al.Strand Synthesis Module (NEB, E7771) and NEBNext Ultra II Directional Second Strand Synthesis Kit (NEB, E7550) according to the manufacturer’s instructions. cDNA fragments were then constructed into libraries using

(6) Complete genomic characterization of global pathogens respiratory syntical virus and human norovirus using probe based capture enrichmentSci RepJuly 1, 2025Sravya V Bhamidipati, Anil Surathu, Hsu Chao, Daniel P. Agustinho, Qin Xiang, Kavya Kottapalli, Abirami Santhanam, Zeineen Momin, Kimberly Walker, Vipin K. Menon, et al.(E7525L; New England Biolabs Inc.) and NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (E7550L; New England Biolabs Inc.). Total RNA (5μl) in a 15μl mixture containing random primers and 2X 1st strand

(7) Transcription can be sufficient, but is not necessary, to advance replication timingbioRxivFebruary 5, 2025Athanasios E. Vouzas, Takayo Sasaki, Juan Carlos Rivera-Mulia, Jesse L. Turner, Amber N. Brown, Karen E. Alexander, Laura Brueckner, Bas van Steensel, David M. GilbertSynthesis Module (NEB, E7771) and the NEBNext Ultra II Directional RNA Second Strand Synthesis Module (NEB, E7550). Libraries were prepared from cDNA using the NEBNext Ultra II Library Prep kit (NEB, E7645), indexed

(8) Associations between circulating amino acids and metabolic dysfunction-associated steatotic liver disease in individuals living with severe obesityPhysiol RepJanuary 27, 2025Ina Maltais-Payette, Jérôme Bourgault, Marie-Frédérique Gauthier, Laurent Biertho, Simon Marceau, François Julien, Patricia L. Mitchell, Christian Couture, Francis Brière, Jacques Corbeil, et al.Synthesis (catalogue #E7771S) and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (catalogue #E7550L, New England BioLabs). cDNA was sequenced on an Illumina NovaSeq S4. The reads were mapped to the human

(9) Intra- and Inter-host Evolution of Human Norovirus in Healthy AdultsbioRxivSeptember 10, 2024Sasirekha Ramani, Sara J. Javornik Cregeen, Anil Surathu, Frederick H. Neill, Donna M. Muzny, Harsha Doddapaneni, Vipin K. Menon, Kristi L. Hoffman, Matthew C. Ross, Ginger Metcalf, et al.and strand-specific libraries were prepared utilizing New England Biolabs Inc. modules (E7525L and E7550L) without rRNA depletion or Poyy A+ RNA isolation steps as previously described [28]. Ilumina unique

(10) Characterizing a complex CT-rich haplotype in intron 4 of SNCA using large-scale targeted amplicon long-read sequencingnpj Parkinson's DiseaseJuly 26, 2024Pilar Alvarez Jerez, Kensuke Daida, Francis P. Grenn, Laksh Malik, Abigail Miano-Burkhardt, Mary B. Makarious, Jinhui Ding, J. Raphael Gibbs, Anni Moore, Xylena Reed, et al.(catalog number E7771L) followed by the NEB Ultra II Directional Second Strand Module (catalog number E7550L). cDNA was converted by standard, ligation-based library preparation and each library amplified for

(11) Evolution of an Extended Pathogenicity Motif in VP2 of Infectious Pancreatic Necrosis Virus Isolates from Farmed Rainbow Trout in TurkeyVirusesJune 20, 2024Cuneyt Tamer, Kristina Ulrich, Nicholas Di Paola, Hanne Nur Kurucay, Harun Albayrak, Manfred WeidmannNEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (New England Biolabs, Ipswich, MA, USA, Cat No: E7550L UK) following the manufacturer’s instructions, and ds cDNAs were cleaned by using RNAClean XP (Beckman Coulter

(12) G-quadruplex folding in Xist RNA antagonizes PRC2 activity for step-wise regulation of X-chromosome inactivationMolecular CellMay 16, 2024Yong Woo Lee, Uri Weissbein, Roy Blum, Jeannie T. Lee,New England Biolabs,Cat#E7645S NEBNext Ultra II directional RNA Second Strand Synthesis Module,New England Biolabs,Cat#E7550S NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina,New England Biolabs,Cat#E6240S NEBNext Poly(A) mRNA Magnetic Isolation Module

(13) Inter and intra-host diversity of RSV in hematopoietic stem cell transplant adults with normal and delayed viral clearanceVirus EvolutionJanuary 22, 2024Vasanthi Avadhanula, Daniel Paiva Agustinho, Vipin Kumar Menon, Roy F Chemaly, Dimpy P Shah, Xiang Qin, Anil Surathu, Harshavardhan Doddapaneni, Donna M Muzny, Ginger A Metcalf, et al.(E7525L; New England Biolabs Inc.) and NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (E7550L; New England Biolabs Inc.). Total RNA in a 15 μl mixture containing random primers and 2X 1st strand

(14) Multi-omics indicators of long-term survival benefits after immune checkpoint inhibitor therapyCell Reports MethodsOctober 23, 2023Jie Zhao, Yiting Dong, Hua Bai, Fan Bai, Xiaoyan Yan, Jianchun Duan, Rui Wan, Jiachen Xu, Kailun Fei, Jie Wang, et al.Synthesis Module,NEB,Cat#E7771L NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module,NEB,Cat#E7550L Hieff NGS Ultima DNA Library Prep Kit for MGI,Yeasen,Cat#13310ES98 DNBSEQ OneStep DNB Make Reagent

(15) T-RHEX-RNAseq – a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cellsBMC GenomicsApril 17, 2023Charlotte Gustafsson, Julia Hauenstein, Nicolai Frengen, Aleksandra Krstic, Sidinh Luc, Robert MånssonStrand Synthesis (NEB cat#E7771) and NEBNext Ultra II Directional RNA Second Strand Synthesis (NEB cat#E7550) modules according to manufacturer’s instructions. In rRNA blocked samples, QIAseq FastSelect -rRNA

(16) Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individualsbioRxivSeptember 22, 2022Harsha Doddapaneni et al.(E7525L; New England Biolabs Inc.) and NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (E7550L; NEB). Total RNA in a 15 μl mixture containing random primers and 2X 1st strand cDNA synthesis buffer

(17) Chromatin organizer SATB1 controls the cell identity of CD4(+) CD8(+) double-positive thymocytes by regulating the activity of super-enhancersNature communicationsSeptember 22, 2022Feng D, Chen Y, Dai R, Bian S, Xue W, Zhu Y, Li Z, Yang Y, Zhang Y, Zhang J, et al.ProtoScript II Reverse Transcriptase and the second-strand cDNA was synthesized using Second Strand Synthesis Enzyme Mix (NEB, E7550L). The purified double-stranded cDNA was subjected to end repair, 3’-dA tailing, and adapter ligation.

(18) Oligonucleotide Capture Sequencing of the SARS-CoV-2 Genome and Subgenomic Fragments from COVID-19 IndividualsbioRxivJune 29, 2022Harsha Doddapaneni et al.(E7525L; New England Biolabs Inc.) and NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (E7550L; NEB). Total RNA in a 15 μl mixture containing random primers and 2X 1st strand cDNA synthesis buffer

(19) Retinoblastoma protein regulates carcinogen susceptibility at heterochromatic cancer driver lociLife science allianceApril 1, 2022Wong KM, King DA, Schwartz EK, Herrera RE, Morrison AJ.preparation was performed with NEBNext Ultra II Directional RNA Second Strand Synthesis Module (Cat. no. E7550; NEB) and NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (Cat. no. E6240; NEB) as instructed

(20) Genomic Profiling of Childhood Tumor Patient-Derived Xenograft Models to Enable Rational Clinical Trial DesignSSRN Electronic JournalApril 1, 2022Jo Lynne Rokita et al.New England Biolabs E7525S NEBNext Ultra Directional RNA Second Strand Synthesis Module New England Biolabs E7550S Uracil-DNA Glycosylase New England Biolabs M0280L Phusion High-Fidelity PCR Master Mix New England Biolabs

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NEBNext Ultra II Directional RNA Second Strand Synthesis Module

NEBNext Ultra II Directional RNA Second Strand Synthesis Module from New England Biolabs

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NEBNext Ultra II Directional RNA Second Strand Synthesis Module from New England Biolabs

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