NEBNext Ultra II Directional RNA Second Strand Synthesis Module E7550S from New England Biolabs

Product Specs
ItemNEBNext Ultra II Directional RNA Second Strand Synthesis Module
CompanyNew England Biolabs
Price $398.00 Inquire
Catalog NumberE7550S
Quantity24 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) Bhlhe40 function in activated B and TFH cells restrains the GC reaction and prevents lymphomagenesisThe Journal of experimental medicineFebruary 7, 2022Rauschmeier R, Reinhardt A, Gustafsson C, Glaros V, Artemov AV, Dunst J, Taneja R, Adameyko I, Månsson R, Busslinger M, et al.RNA First Strand Synthesis (E7771; NEB) and NEBNext Ultra II Directional RNA Second Strand Synthesis (E7550; NEB) modules. QIAseq FastSelect rRNA HMR kit (Qiagen) was used to minimize ribosomal RNA contribution

(22) Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programsScience AdvancesDecember 24, 2021Xiaotong Wu et al.NEBNext first-strand (NEB, catalog no. E7771S) and second-strand synthesis modules (NEB, catalog no. E7550S) and then purified with AMPure XP beads (Beckman Coulter, catalog no. A63882). Synthesized cDNA was

(23) Modifying the m(6)A brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic taggingMolecular psychiatryDecember 1, 2021Martinez De La Cruz B, Markus R, Malla S, Haig MI, Gell C, Sang F, Bellows E, Sherif MA, McLean D, Lourdusamy A, et al.NEBNext RNA First strand synthesis module (NEB, E7525S) and Ultra-Directional RNA Second Strand Synthesis Module (NEB, E7550S) respectively. Sequencing libraries from double stranded cDNA were generated using NEBNext® Ultra II DNA Library Prep Kit for Illumina

(24) Limited access to antigen drives generation of early B cell memory while restraining the plasmablast responseImmunitySeptember 14, 2021Glaros V, Rauschmeier R, Artemov AV, Reinhardt A, Ols S, Emmanouilidi A, Gustafsson C, You Y, Mirabello C, Björklund ÅK, et al.Strand Synthesis (NEB Cat E7771) and NEBNext Ultra II Directional RNA Second Strand Synthesis (NEB Cat E7550) modules. QIAseq FastSelect rRNA HMR kit (QIAGEN) was utilized to minimize rRNA contribution to the

(25) Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individualsPLOS ONEAugust 25, 2021Harsha Doddapaneni et al.(E7525L; New England Biolabs Inc.) and NEBNext® Ultra™ II Directional RNA Second Strand Synthesis Module (E7550L; New England Biolabs Inc.). Total RNA in a 15 μl mixture containing random primers and 2X 1st strand

(26) EmPC-seq: Accurate RNA-sequencing and Bioinformatics Platform to Map RNA Polymerases and Remove Background ErrorBIO-PROTOCOLFebruary 20, 2021Yuqing Wang, Tin Hang Chong, Ilona Christy Unarta, Xinzhou Xu, Gianmarco D Suarez, Jiguang Wang, John T Lis, Xuhui Huang, Peter Pak-Hang Cheung18080051) 40. NEBNext® UltraTM II Directional RNA Second Strand Synthesis Module (NEB, catalog number: E7550L) 41. MinElute PCR Purification Kit Print (QIAGEN, catalog number: 28004) 42. NEBNext® Ultra TM II DNA Library Prep

(27) Cystic Fibrosis Lung Transplant Recipients Have Suppressed Airway Interferon Responses during Pseudomonas InfectionCell Reports MedicineAugust 6, 2020Daniel T. Dugger et al.NEBNext RNA First Strand Synthesis Module,New England BioLabs,Cat#E7525 NEBNext Ultra® Second Strand Synthesis Module,New England BioLabs,Cat#E7550 NEBNext Ultra® II DNA Library Prep Kit for Illumina,New England BioLabs,Cat#E7645 GenElute Mammalian Genomic DNA Miniprep

(28) High‐throughput, microscope‐based sorting to dissect cellular heterogeneityMolecular Systems BiologyJune 5, 2020Nicholas Hasle et al.Synthesis (Thermo Fisher 18091050) and NEBNext Ultra II Directional RNA Second Strand Synthesis (NEB E7550), according to the manufacturer's instructions. cDNA was then tagmented with Nextera Tn5 (Illumina;

(29) Genomic Repeats Categorize Genes with Distinct Functions for Orchestrated RegulationCell ReportsMarch 10, 2020J. Yuyang Lu et al.First Strand Synthesis Module,NEB,Cat# E7771L NEBNext Ultra II Second Strand Synthesis Module,NEB,Cat# E7550L NEBNext Ultra II DNA Library Prep Kit,NEB,Cat# E7645 Strep-Tactin XT purification system,IBA Lifesciences,Cat#

(30) Genomic Profiling of Childhood Tumor Patient-Derived Xenograft Models to Enable Rational Clinical Trial DesignCell ReportsNovember 5, 2019Jo Lynne Rokita et al.,New England Biolabs,E7525S NEBNext Ultra Directional RNA Second Strand Synthesis Module,New England Biolabs,E7550S Uracil-DNA Glycosylase,New England Biolabs,M0280L Phusion High-Fidelity PCR Master Mix,New England Biolabs

(31) Diverse Long RNAs Are Differentially Sorted into Extracellular Vesicles Secreted by Colorectal Cancer CellsCell reportsOctober 16, 2018Hinger SA, Cha DJ, Franklin JL, Higginbotham JN, Dou Y, Ping J, Shu L, Prasad N, Levy S, Zhang B, et al.,New England Biolabs,Cat# E7525S NEBNext Ultra Directional Second Strand Synthesis Kit,New England Biolabs,Cat# E7550S NEBNext DNA Library Prep Master Mix Set,New England Biolabs,Cat# E6040S KAPA Biosystems Library Quantification

(32) PacBio-Based Mitochondrial Genome Assembly of Leucaena trichandra (Leguminosae) and an Intrageneric Assessment of Mitochondrial RNA EditingGenome Biology and EvolutionSeptember 1, 2018Lynsey Kovar et al.RNA First Strand Synthesis Module (#E7771), Ultra II Directional RNA Second Strand Synthesis Module (#E7550), Ultra II DNA Library Prep Kit for Illumina (#E7645), and Multiplex Oligos for Illumina (Index Primers

(33) Mass Azithromycin Distribution and Community Microbiome: A Cluster-Randomized TrialOpen Forum Infectious DiseasesAugust 1, 2018Thuy Doan et al.NEBNext RNA First Strand Synthesis Module (E7525) and NEBNext Ultra Directional RNA Second Strand Synthesis Module (E7550) to generate double-stranded cDNA. The cDNA was converted to Illumina libraries using the NEBNext Ultra

(34) Metagenomic deep sequencing of aqueous fluid detects intraocular lymphomasBritish Journal of OphthalmologyJanuary 1, 2018John Gonzales, Thuy Doan, Jessica G Shantha, Michele Bloomer, Michael R Wilson, Joseph L DeRisi, Nisha AcharyaNEBNext RNA First Strand Synthesis Module (E7525) and NEBNext Ultra Directional RNA Second Strand Synthesis Module (E7550) to generate doublestranded cDNA. The cDNA was converted to Illumina libraries using the NEBNext Ultra

(35) ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germlineNucleic acids researchSeptember 6, 2017Brown KC, Svendsen JM, Tucci RM, Montgomery BE, Montgomery TA.18080-093). Second strand cDNA was synthesized using the NEBNext Second Strand Synthesis Module (NEB, E7550S), which uses dUTP instead of dTTP to preserve strand information. Double-stranded cDNA was end repaired

(36) A Randomized Phase II Neoadjuvant Study of Cisplatin, Paclitaxel With or Without Everolimus in Patients with Stage II/III Triple-Negative Breast Cancer (TNBC): Responses and Long-term Outcome Correlated with Increased Frequency of DNA Damage Response Gene Mutations, TNBC Subtype, AR Status, and Ki67Clinical Cancer ResearchAugust 1, 2017Bojana Jovanović et al.Directional rRNA Reduction RNA-seq library reactions on all samples (Illumina, cat# MRZE724 and NEB cat# E7550L). All samples were sequenced to a depth of 50e6 reads using the Illumina HiSeq 2500 on high output mode

(37) A novel cause of chronic viral meningoencephalitis: Cache Valley virusAnnals of NeurologyJuly 25, 2017Michael R. Wilson et al.NEBNext RNA First Strand Synthesis Module (E7525) and NEBNext Ultra Directional RNA Second Strand Synthesis Module (E7550) to generate double‐stranded complementary DNA (cDNA). The cDNA was converted to Illumina (San Diego,

(38) Illuminating uveitis: metagenomic deep sequencing identifies common and rare pathogensGenome MedicineDecember 1, 2016Thuy Doan et al.NEBNext RNA First Strand Synthesis Module (E7525) and NEBNext Ultra Directional RNA Second Strand Synthesis Module (E7550) to generate double-stranded cDNA. The cDNA was converted to Illumina libraries using the NEBNext Ultra

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NEBNext Ultra II Directional RNA Second Strand Synthesis Module

NEBNext Ultra II Directional RNA Second Strand Synthesis Module from New England Biolabs

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NEBNext Ultra II Directional RNA Second Strand Synthesis Module from New England Biolabs

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