ELISpot Flex: Mouse IFN-γ (ALP) 3321-2A from Mabtech AB

Product Specs
ItemELISpot Flex: Mouse IFN-γ (ALP)
CompanyMabtech AB
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3321-2A
Reactivitymouse
MethodELISpot
Quantityfor 4 plate
ApplicationsELISpot
Detection TargetIFN-γ
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFN-g, If2f, Ifg
Conjugate/Tag/LabelALP
TypeStreptavidin-ALP
Formatwithout plates
ComponentsCapture mAb (AN18),Biotinylated detection mAb (R4-6A2),Streptavidin-ALP

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Mabtech AB

Box 1233

Nacka Strand, SE-131 28

Sweden

Phone: +46 8 716 27 00
+46 8 716 27 01

Company Profile

Website: http://www.mabtech.com

(1) Immunogenicity and Therapeutic Efficacy of a Sendai-Virus-Vectored HSV-2 Vaccine in Mouse and Guinea Pig ModelsVaccines (Basel)November 24, 2023Xiuxiu Ren, Wenhao Su, Shishi Li, Tingting Zhao, Qiufang Huang, Yinan Wang, Xiaojie Wang, Xiaohuan Zhang, Jiangbo Weiwere resuspended, counted, and adjusted to 1 × 107 cell/mL with RPMI 1640 ready for use. IFN-γ (Cat.No.3321-2A, Mabtech, Nacka Strand, Sweden), IL-2 (Cat.No.3441-2A, Mabtech,), and IL-4 (Cat.No.3311-2A, Mabtech,)

(2) Development of HPV16 mouse and dog models for more accurate prediction of human vaccine efficacyLaboratory Animal ResearchJune 12, 2023Emmanuelle Totain, Loïc Lindner, Nicolas Martin, Yolande Misseri, Alexandra Iché, Marie-Christine Birling, Tania Sorg, Yann Herault, Alain Bousquet-Melou, Pascale Bouillé, et al.the manufacturer's recommendations (Mabtech). For murine splenocytes, the Mouse IFN-γ ELISpot BASIC (3321-2A) kit was used. For canine PBMCs, the horse IFN-gamma ELISpot BASIC (3117-2A) kit was used. In brief,

(3) COVID-19 mRNA vaccine protects against SARS-CoV-2 Omicron BA.1 infection in diet-induced obese mice through boosting host innate antiviral responsesEBioMedicineMarch 1, 2023Chen Y, Song W, Li C, Wang J, Liu F, Ye Z, Ren P, Tong Y, Li J, Ou Z, et al.protein at 37 °C for 48 h, IFN-γ producing cells were determined using a mouse IFN-γ ELISpot BASIC kit (3321-2A, Mabtech, Inc., Stockholm, Sweden) following the manufacturer's instructions.32 RNA sequencing and

(4) Germline IgM predicts T cell immunity to PneumocystisJCI InsightSeptember 8, 2022Kristin Noell, Guixiang Dai, Dora Pungan, Anna Ebacher, Janet E. McCombs, Samuel J. Landry, Jay K. Kolls21). Mice were euthanized at week 2 or week 6 for lung IFN-γ Elispsot assay (MabTech, product code 3321-2A) (13) as a measure of Th1 response or measurement of IL-5 in supernatant for type 2 responses using

(5) CD47 blockade enhances the efficacy of intratumoral STING-targeting therapy by activating phagocytesThe Journal of experimental medicineNovember 1, 2021Kosaka A, Ishibashi K, Nagato T, Kitamura H, Fujiwara Y, Yasuda S, Nagata M, Harabuchi S, Hayashi R, Yajima Y, et al.T cell numbers were visualized by anti-mouse IFN-γ enzyme-linked immunospot assay (ELISpot, catalog no. 3321-2A; Mabtech) according to the manufacturer’s protocol. BCIP/NBT plus substrate (Mabtech) was used for detection.

(6) T cell-mediated anti-tumor immunity cooperatively induced by TGFβR1 antagonism and gemcitabine counteracts reformation of the stromal barrier in pancreatic cancerMolecular Cancer TherapeuticsOctober 1, 2021Dandan Li et al.immobilized cytokine as ImmunoSpots was carried out according to the manufacturer's instructions (catalog no. 3321–2A, Mabtech), ELISpots were read and quantified in an ImmunoSpot S6 universal analyzer. T regulatory cells

(7) Single-dose intranasal vaccination elicits systemic and mucosal immunity against SARS-CoV-2iScienceSeptember 24, 2021An X, Martinez-Paniagua M, Rezvan A, Sefat SR, Fathi M, Singh S, Biswas S, Pourpak M, Yee C, Liu X, et al.assays,Critical commercial assays,Critical commercial assays ,, Mouse IFN-γ ELISpot BASIC kit (ALP),Mabtech,Cat#3321-2A Mouse IgG/IgA Double-Color ELISPOT,ImmunoSpot,Cat#mIgGIgA-DCE-1M/2 IgA Mouse Uncoated ELISA Kit,Invitrogen,Cat#88-50450-88 BD™

(8) Inclusion of cGAMP within virus-like particle vaccines enhances their immunogenicityEMBO reportsAugust 4, 2021Chauveau L, Bridgeman A, Tan TK, Beveridge R, Frost JN, Rijal P, Pedroza-Pacheco I, Partridge T, Gilbert-Jaramillo J, Knight ML, et al.UFC500396 2′‐3′ cGAMP ELISA kit,Cayman chemical,Cat # 501700 Mouse IFNγ ELISPOT BASIC (ALP) kit,Mabtech,Cat # 3321‐2A Mouse IgG Basic ELISPOT BASIC (ALP) kit,Mabtech,Cat # 3825‐2A Red blood cell lysis buffer,Sigma,Cat

(9) A Novel HAGE/WT1-ImmunoBody® Vaccine Combination Enhances Anti-Tumour Responses When Compared to Either Vaccine AloneFrontiers in OncologyJune 28, 2021Rukaia Almshayakhchi, Divya Nagarajan, Jayakumar Vadakekolathu, Barbara-Ann Guinn, Stephen Reeder, Victoria Brentville, Rachael Metheringham, A. Graham Pockley, Lindy Durrant, Stephanie McArdleharvested from immunised mice in an ex vivo IFN-γ ELISpot assay (ELISpot kit for mouse IFN-γ/MABTECH, 3321-2A). The assay was performed as previously described (29). Briefly 0.5x106 of freshly isolated splenocytes

(10) Recombinant oncolytic adenovirus expressing a soluble PVR elicits long-term antitumor immune surveillanceMolecular therapy oncolyticsMarch 26, 2021Zhang H, Zhang Y, Dong J, Li B, Xu C, Wei M, Wu J, Wei J.USA). IFN-γ ELISpot assay Immune activity in the TME was measured with a mouse IFN-γ ELISpot assay kit (3321-2A, Mabtech, Sweden), according to the kit protocol. In brief, the plate was coated with mouse IFN-γ capture

(11) SARS-CoV-2 spike glycoprotein vaccine candidate NVX-CoV2373 immunogenicity in baboons and protection in miceNature communicationsJanuary 14, 2021Tian JH, Patel N, Haupt R, Zhou H, Weston S, Hammond H, Logue J, Portnoff AD, Norton J, Guebre-Xabier M, et al.were performed following manufacturer’s procedures for mouse IFN-γ and IL-5 ELISpot kits (3321-2H and 3321-2A, Mabtech, Cincinnati, OH). Briefly, 105 splenocytes in a volume of 200 µL were stimulated with NVX-CoV2373

(12) Development of a multi-antigenic SARS-CoV-2 vaccine candidate using a synthetic poxvirus platformNature communicationsNovember 30, 2020Chiuppesi F, Salazar MD, Contreras H, Nguyen VH, Martinez J, Park Y, Nguyen J, Kha M, Iniguez A, Zhou Q, et al.ELISpot T-cell detection by IFNγ ELISpot assay was performed according to the manufacturer’s instructions (3321-2A, Mabtech). ELISpot PVDF plates (MSIPS4W10, Millipore) were pre-activated with ethanol and coated with

(13) Japanese Encephalitis Virus Vaccination Elicits Cross-Reactive HLA-Class I-Restricted CD8 T Cell Response Against Zika Virus InfectionFrontiers in ImmunologySeptember 25, 2020Marion Tarbe et al.ELISpot assay. Mouse IFNγ ELISpot Assay Mouse IFNγ ELISpot assay was performed using the ELISpot kit (3321-2A, Mabtech) according to the manufacturer’s instruction. Briefly, the day before the experiment, plates

(14) CD4⁺ T Cells Recognize Conserved Influenza A Epitopes through Shared Patterns of V-Gene Usage and Complementary Biochemical FeaturesCell reportsJuly 14, 2020Greenshields-Watson A, Attaf M, MacLachlan BJ, Whalley T, Rius C, Wall A, Lloyd A, Hughes H, Strange KE, Mason GH, et al.Commercial Assays ,, IFN-γ ELISpot (human),Mabtech,Cat#3420-2A IFN-γ ELISpot (mouse),Mabtech,Cat#3321-2A BirA biotin-protein ligase kit,Avidity,Cat#BirA500 Pierce Protein A IgG Plus Orientation Kit,Thermo Fisher

(15) An HER2-Displaying Virus-Like Particle Vaccine Protects from Challenge with Mammary Carcinoma Cells in a Mouse ModelVaccinesMay 20, 2019Nika L, Cuadrado-Castano S, Asthagiri Arunkumar G, Grünwald-Gruber C, McMahon M, Koczka K, García-Sastre A, Krammer F, Grabherr R.interferon (IFN)γ ELISpot assay was performed according to the manufacturer’s instructions (MabTech, 3321-2A). Briefly, ELISpot plates (EMD Millipore, MAIPS4510) were coated with 15 µg/mL antimouse IFNγ capture

(16) Fatty acid transport protein 2 reprograms neutrophils in cancerNatureMay 1, 2019Veglia F, Tyurin VA, Blasi M, De Leo A, Kossenkov AV, Donthireddy L, To TKJ, Schug Z, Basu S, Wang F, et al.

(17) The Genetic Adjuvants Interleukin-12 and Granulocyte-Macrophage Colony Stimulating Factor Enhance the Immunogenicity of an Ebola Virus Deoxyribonucleic Acid Vaccine in MiceThe Journal of infectious diseasesNovember 22, 2018Suschak JJ, Bagley K, Shoemaker CJ, Six C, Kwilas S, Dupuy LC, Schmaljohn CS.

(18) CD8⁺ T cells mediate the antitumor activity of frankincense and myrrh in hepatocellular carcinomaJournal of translational medicineMay 21, 2018Xu C, Lu X, Liu W, Chen A, Meng G, Zhang H, Li B, Zhang Y, Wu J, Wei J.enzyme‑linked immunosorbent spot (ELISpot) assay Immunity activity in the TME was measured with mouse IFN-γ ELISpot Kit (3321-2A, Mabtech, Sweden) according to the guidelines. In brief, the plate was coated with mouse INF-γ capture

(19) Promoting the accumulation of tumor-specific T cells in tumor tissues by dendritic cell vaccines and chemokine-modulating agentsNature protocolsFebruary 1, 2018Obermajer N, Urban J, Wieckowski E, Muthuswamy R, Ravindranathan R, Bartlett DL, Kalinski P.

(20) Neoadjuvant oncolytic virotherapy before surgery sensitizes triple-negative breast cancer to immune checkpoint therapyScience translational medicineJanuary 3, 2018Bourgeois-Daigneault MC, Roy DG, Aitken AS, El Sayes N, Martin NT, Varette O, Falls T, St-Germain LE, Pelin A, Lichty BD, et al.

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ELISpot Flex: Mouse IFN-γ (ALP) from Mabtech AB

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Mabtech AB

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ELISpot Flex: Mouse IFN-γ (ALP) from Mabtech AB

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