ELISpot Pro: Human IFN-γ (ALP) 3420-2APT-2 from Mabtech AB

Product Specs
ItemELISpot Pro: Human IFN-γ (ALP)
CompanyMabtech AB
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3420-2APT-2
Reactivityhuman
Quantity2 plate
MethodELISpot
ApplicationsELISpot
Detection TargetIFN-γ
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFG, IFI, IMD69
Conjugate/Tag/LabelALP
TypeOne-step ALP
Formatpre-coated plates
ComponentsAnti-CD3 mAb,Substrate (BCIP/NBT-plus),Pre-coated MSIP clear plates (mAb 1-D1K),Enzyme-conjugated detection mAb (7-B6-1-ALP)

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Mabtech AB

Box 1233

Nacka Strand, SE-131 28

Sweden

Phone: +46 8 716 27 00
+46 8 716 27 01

Company Profile

Website: http://www.mabtech.com

(1) Evaluation of T cell responses to naturally processed variant SARS-CoV-2 spike antigens in individuals following infection or vaccinationCell ReportsMay 30, 2023Zixi Yin, Ji-Li Chen, Yongxu Lu, Beibei Wang, Leila Godfrey, Alexander J. Mentzer, Xuan Yao, Guihai Liu, Dannielle Wellington, Yiqi Zhao, et al.Commercial Assays,Critical Commercial Assays,Critical Commercial Assays ,, ELISpot assay,Mabtech AB,Cat#3420-2APT-2 ,, Experimental Models: Cell Lines,Experimental Models: Cell Lines,Experimental Models: Cell Lines ,, SARS-CoV-2

(2) Safety and immunogenicity of an inactivated whole virion SARS-CoV-2 vaccine, TURKOVAC, in healthy adults: Interim results from randomised, double-blind, placebo-controlled phase 1 and 2 trialsVaccineJanuary 9, 2023Aykut Ozdarendeli, Zafer Sezer, Shaikh Terkis Islam Pavel, Ahmet Inal, Hazel Yetiskin, Busra Kaplan, Muhammet Ali Uygut, Adnan Bayram, Mumtaz Mazicioglu, Gamze Kalin Unuvar, et al.response at day 43, by interferon (IFN)-γ ELISPOT assay using a commercial kit (Human IFN-γ ELISpotPRO [3420-2APT-2]; Mabtech, Sweden). The results were expressed as the number of spot-forming cells (SFCs) per 100 000

(3) Antibody decay, T cell immunity and breakthrough infections following two SARS-CoV-2 vaccine doses in inflammatory bowel disease patients treated with infliximab and vedolizumabNature CommunicationsDecember 1, 2022Simeng Lin, Nicholas A. Kennedy, Aamir Saifuddin, Diana MuÃ±oz Sandoval, Catherine J. Reynolds, Rocio Castro Seoane, Sherine H. Kottoor, Franziska P. Pieper, Kai-Min Lin, David K. Butler, et al.specific T cell responses IFN-γ T cell ELISpot assays were performed using pre-coated plates (Mabtech 3420-2APT) and using the protocol described previously28,32. Two-hundred thousand cells were seeded per well and

(4) COVID-19 vaccine-induced antibody and T-cell responses in immunosuppressed patients with inflammatory bowel disease after the third vaccine dose (VIP): a multicentre, prospective, case-control studyThe Lancet Gastroenterology & HepatologyNovember 1, 2022James L Alexander, Zhigang Liu, Diana MuÃ±oz Sandoval, Catherine Reynolds, Hajir Ibraheim, Sulak Anandabaskaran, Aamir Saifuddin, Rocio Castro Seoane, Nikhil Anand, Rachel Nice, et al.after the third vaccine dose. IFN-γ T-cell ELISpot assays were done using precoated plates (Mabtech 3420-2APT, MabTech, Nacka Strand, Sweden) and using the protocol described previously.6, 20, 21 200 000 cells

(5) Immunodeficiency syndromes differentially impact the functional profile of SARS-CoV-2-specific T cells elicited by mRNA vaccinationImmunitySeptember 13, 2022Yu Gao et al.(<1%). The assay was performed using pre-coated plates and reagents from a human IFN-γ ELISpotPRO kit (3420-2APT-2, Mabtech). PBMCs were stimulated for 20 hours in assay plates incubated at 37°C and 5% CO2. Spots were

(6) Immune boosting by B.1.1.529 ( Omicron) depends on previous SARS-CoV-2 exposureScienceJuly 15, 2022Catherine J. Reynolds et al.

(7) Effectiveness and Durability of COVID-19 Vaccination in 9447 Patients With IBD: A Systematic Review and Meta-AnalysisClinical Gastroenterology and HepatologyJuly 1, 2022Anuraag Jena, Deepak James, Anupam K. Singh, Usha Dutta, Shaji Sebastian, Vishal Sharma,Anti-SARS-CoV-2 spike T cell responses: IFN-γ T cell ELISpot assays were performed using pre-coated plates (Mabtech 3420-2APT).A response below 2 SDs of the media only control wells was deemed to be a null response Mayorga Ayala

(8) Synergistic Therapeutic Effects of Low Dose Decitabine and NY-ESO-1 Specific TCR-T Cells for the Colorectal Cancer With Microsatellite StabilityFrontiers in OncologyJune 14, 2022Ganjun Yu, Wenying Wang, Xiaobo He, Jia Xu, Rongrong Xu, Tao Wan, Yanfeng WuUSA) at 1:4000 dilution. IFN-γ ELISPOT Assay For IFN-γ production assay by ELISPOT kit (MabTech, 3420-2APT-2, USA), untreated T cells, and T cells transduced with mock or TCR gene (ctrl-T or TCR-T) were used as

(9) HLA‐DR polymorphism in SARS‐CoV‐2 infection and susceptibility to symptomatic COVID‐19ImmunologyMay 1, 2022Stuart Astbury et al.described [19, 20, 21]. T‐cell ELISpot analysis was carried out using pre‐coated ELISpot plates (Mabtech 3420‐2APT), read on an AID classic ELISpot plate reader (Autoimmun Diagnostika GMBH) and analysed as previously

(10) Elevated CD21low B Cell Frequency Is a Marker of Poor Immunity to Pfizer-BioNTech BNT162b2 mRNA Vaccine Against SARS-CoV-2 in Patients with Common Variable ImmunodeficiencyJournal of Clinical ImmunologyMay 1, 2022Peter Bergman, David Wullimann, Yu Gao, Emilie Wahren Borgström, Anna-Carin Norlin, Sara Lind Enoksson, Soo Aleman, Hans-Gustaf Ljunggren, Marcus Buggert, C. I. Edvard Smithaccording to the manufacturer’s protocol using plates and reagents from a human IFN-γ ELISpotPRO kit (3420-2APT-2, Mabtech). PBMCs were stimulated for 20 h in assay plates incubated at 37 °C and 5% CO2. Spots were

(11) T-cell immune responses following vaccination with mRNA BNT162b2 against SARS-CoV-2 in patients with chronic lymphocytic leukemia: results from a prospective open-label clinical trialHaematologicaApril 1, 2022Lisa Blixt et al.ELISpot assay was applied as previously described,11 using plates and reagents from a human IFNg ELISpot kit (3420-2APT-2, Mabtech). Briefly, 2.5x105 peripheral blood mononuclear cells (PBMC)/well were seeded and supplemented

(12) A Personalized Neoantigen Vaccine in Combination with Platinum-Based Chemotherapy Induces a T-Cell Response Coinciding with a Complete Response in Endometrial CarcinomaCancersNovember 18, 2021Harari A, Sarivalasis A, de Jonge K, Thierry AC, Huber F, Boudousquie C, Rossier L, Orcurto A, Imbimbo M, Baumgaertner P, et al.IFNγ Enzyme-Linked ImmunoSpot (ELISpot) assays were performed using pre-coated 96-well ELISpot plates (3420-2APT-10 Mabtech, Stockholm, Södermanland and Uppland, Sweden) [37]. Then, 100,000 cells were re-challenged (in

(13) Prediction of neo-epitope immunogenicity reveals TCR recognition determinants and provides insight into immunoeditingCell Reports MedicineFebruary 16, 2021Julien Schmidt et al.

(14) Discordant neutralizing antibody and T cell responses in asymptomatic and mild SARS-CoV-2 infectionScience ImmunologyDecember 23, 2020Catherine J. Reynolds et al.involving T cell stimulation with proteins or MEP peptide pools, pre-coated ELISpot plates (Mabtech 3420-2APT) were washed x4 with sterile PBS and were blocked with R10 for 1h at room temperature. 200,000 PBMC were

(15) COVID-19 vaccine BNT162b1 elicits human antibody and TH1 T cell responsesNatureOctober 22, 2020Ugur Sahin et al.systems Fixable Viability Dye/eF780/eBioscience/65-0865-14/2185428/1:1,666 ELISpotPro kit/cat. no. 3420-2APT-10/lot no. 370/Mabtech: Primary anti-IFNg antibody/clone c1-D1K/pre-coated plates Secondary anti-IFNg antibody/clone

(16) Robust T Cell Immunity in Convalescent Individuals with Asymptomatic or Mild COVID-19CellOctober 1, 2020Sekine T, Perez-Potti A, Rivera-Ballesteros O, Strålin K, Gorin JB, Olsson A, Llewellyn-Lacey S, Kamal H, Bogdanovic G, Muschiol S, et al.

(17) Immune recognition of somatic mutations leading to complete durable regression in metastatic breast cancerNature MedicineJune 4, 2018Nikolaos Zacharakis et al.

(18) An immunogenic personal neoantigen vaccine for patients with melanomaNatureJuly 13, 2017Ott PA, Hu Z, Keskin DB, Shukla SA, Sun J, Bozym DJ, Zhang W, Luoma A, Giobbie-Hurder A, Peter L, et al.

(19) Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapyNatureJune 16, 2016Kranz LM, Diken M, Haas H, Kreiter S, Loquai C, Reuter KC, Meng M, Fritz D, Vascotto F, Hefesha H, et al.

(20) Checkpoint blockade cancer immunotherapy targets tumour-specific mutant antigensNatureNovember 27, 2014Gubin MM, Zhang X, Schuster H, Caron E, Ward JP, Noguchi T, Ivanova Y, Hundal J, Arthur CD, Krebber WJ, et al.

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ELISpot Pro: Human IFN-γ (ALP) from Mabtech AB

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ELISpot Pro: Human IFN-γ (ALP) from Mabtech AB

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