Gibson Assembly Cloning Kit E5510S from New England Biolabs

Product Specs
ItemGibson Assembly Cloning Kit
CompanyNew England Biolabs
Price $207.00 Inquire
Catalog NumberE5510S
Quantity10 reactions
ApplicationsDNA Manipulation
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) hnRNPUL1 has a dead polynucleotide kinase domain that regulates RNA and protein interactionsiScienceApril 17, 2026Carmen V. Apostol, Ang Li, Peter Daniels, Llywelyn Griffith, Johnathan Cooper-Knock, Elisa Aguilar-Martinez, Ivaylo D. Yonchev, Ashleigh G.R. Whelan, Pamela J. Shaw, Ian M. Sudbery, et al.commercial assays,Critical commercial assays ,, Gibson assembly cloning kit,New England Biolabs,Cat# E5510S Q5 Site-Directed Mutagenesis Kit,New England Biolabs,Cat# E0554S ,, Deposited data,Deposited data,Deposited

(2) Heparan sulfate is essential for Drosophila FGF exportbioRxivApril 6, 2026Guilherme Oliveira Barbosa, Christian Solis-Calero, Thomas B. KornbergC-terminal to the FGF domain of Bnl (Sohr et al., 2019), using Gibson Assembly Cloning Kit (NEB Cat#: E5510S), Q5® High-Fidelity DNA Polymerase (NEB #M0491) and pAc5.1/V5-His A linearized by XhoI digestion. Based

(3) Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cellsMolecular Therapy OncologyMarch 19, 2026Juliane Mietz, Lukas Egli, Meike Misiune, Arianna Picozzi, Nicolò Coianiz, Danielle Thompson, Marianna Ponzo, Alisson Charmey, Beat Bornhauser, Chiara F. Magnani, et al.(New England Biolabs, Cat. R3101)-linearized pUC19 (Addgene, Cat. #50005) by Gibson Assembly (New England Biolabs, Cat. E5510S) and introduced into competent NEB5alpha cells (New England Biolabs, Cat. C2987) by heat shock. AAV6

(4) RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1Nucleic Acids ResMarch 17, 2026Parnia Babaei, Alice O Coomer, Kaliya Georgieva, Giulia Guiducci, Elisa Vitiello, Martin Dodel, Eleni Maniati, Hanya Elsayed Eid, Anisha Thind, Sneha Krishnamurthy, et al.cloned upstream of the MS2 stem-loop sequence of the 10xMS2 vector using a Gibson assembly cloning kit (E5510S, NEB) at 50°C for 15 min. The assembled C1QTNF1–AS1–10xMS2 vector was transformed into competent Escherichia coli

(5) Integrating natural and engineered genetic variations to decode regulatory influence on blood traitsCell ReportsFebruary 24, 2026Manuel Tardaguila, Dominique Von Schiller, Michela Colombo, Ilaria Gori, Eve L. Coomber, Thomas Vanderstichele, Paola Benaglio, Chiara Chiereghin, Sebastian Gerety, Dragana Vuckovic, et al.Buffer,Roche,Cat#KK5516 pGEM T easy system,Promega,Cat#A1360 Gibson Assembly® Cloning Kit,New England Biolabs,Cat#E5510S KAPA Illumina SYBR Universal Lib Q. Kit,Roche,Cat#07960140001 MiSeq Reagent Kit v2 300 cycle,Illumina,Cat#MS-102-2002 Qubit

(6) Membrane-associated periodic skeleton regulates major forms of endocytosis in neurons through a signaling-driven positive feedback loopSci AdvFebruary 13, 2026Jinyu Fei, Yuanmin Zheng, Caden LaLonde, Yuan Tao, Ruobo Zhouexpression vector FUGW (Addgene, plasmid #14883), through Gibson assembly reaction (New England Biolabs, E5510S) according to the manufacturer’s instructions. Coding sequences for CAV1, FLOT1, SH3GL1 (synthesized

(7) PHLDA1 is a shared diagnostic and key mediator of inflammatory fibrosis in heart and kidneyFrontiers in ImmunologyFebruary 5, 2026Lei Hua, Liangru Shen, Yongshou Tao, Chentong Wang, Xiaohang Shaosubsequent experiments, both cell types were immortalized through lentiviral transduction of SV40LT (E5510S, New England Biolabs) and following our previous protocol (21). Immortalized cell types were cultured

(8) Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epibolyDevelopmentFebruary 1, 2026Haoyu Wan, Sifa Quibria, Ernest Iu, Sirma Damla User, Sergey V. Plotnikov, Ashley E. E. BrucepFP2 using the Gibson assembly method (Gibson et al., 2009) with the Gibson Assembly Cloning Kit (NEB, E5510S). For yolk-specific expression of full-length Camsap2a, the plasmid pzTol2[Exp]-{wnt8 promoter}>{camsap2a}

(9) Tunable Chemical and Optical Control of ER-Plasma Membrane Contact Site Geometry and Dynamics with High-Fidelity VisualizationbioRxivJanuary 31, 2026Shaoqing Zhang, Jinyu Fei, Yuanmin Zheng, Ruobo Zhou(New England Biolabs, M0491L), and plasmids were assembled using Gibson assembly (New England Biolabs, E5510S) according to the manufacturer’s instructions. Unless otherwise noted, constructs were cloned into mammalian expression

(10) BAF complex-independent gene activation by SS18::SSXbioRxivJanuary 31, 2026Afroditi Sotiriou, Jinxiu Li, Sanya Middha, Jake A. Ward, Selina Troester, Lianghao Mao, Martin Schneider, Dario Frey, Eliza C. Wray, Sara Bocedi, et al.plasmid. For cloning of the miniTurbo-V5-SS18::SSX2 plasmid, SS18::SSX2 was cloned by Gibson assembly (NEB, E5510S) into BamHI (NEB) digested pENTR221_miniTurbo_V546. Primers to amplify SS18::SSX2 contained Gibson compatible

(11) Dual-functioning Targeted ADAM17 Blocker CD16 (TAB16) mediates selective ADAM17 inhibition in NK cells and engages overexpressed ADAM17 in tumor cells to induce cytotoxicityFrontiers in ImmunologyJanuary 30, 2026Kate J. Dixon, Robert Hullsiek, Sam Wang, Ryan R. Friess, Anders W. Matson, Yvette Soignier, Alexander J. Lenvik, Jianming Wu, Geoffrey T. Hart, Martin Felices, et al.backbone using the Gibson Assembly HiFi Kit (New England Biolabs, Ipswich, Massachusetts, USA, Cat. No. E5510S) according to the manufacturer’s instructions. E. coli DH5-α transformants were selected on kanamycin,

(12) Microtubule detyrosination links inflammasome activation to apoptotic cell death in macrophages upon influenza A virus infectionJournal of VirologyJanuary 20, 2026Joyeeta Kar, Mikako Hirohama, Kotono Nakayama, SangJoon Lee, Atsushi Kawaguchi5′-GATCCTTGCGGCCGCTCAGACCCGGATCTGGTACCCGT-3′, and then cloned into the pCDH plasmid using Gibson Assembly reagent (New England Biolabs; E5510S). The cDNA of VASH1ΔN was amplified from pCDH-GFP-VASH1 as a template using the primers (5′-CCGTCTACACCTGTCCCTGA-3′

(13) A Chemically Inducible Multimerization System for Tunable and Background-Free RTK ActivationAnalytical ChemistryJanuary 20, 2026Yuanmin Zheng, Jinyu Fei, Abhirup Chakrabarti, Ruobo Zhousynthesized by commercial providers. Constructs were assembled using Gibson assembly (New England Biolabs, E5510S) according to the manufacturer’s instructions. The coding sequence for human FGFR1 was obtained from

(14) Molecular determinants of Smc5/6 association with DNA junctionsNature CommunicationsJanuary 17, 2026Jeremy T-H. Chang, Victoria Miller-Browne, Gabriella N. L. Chua, Jian Zheng, Emily C. Beckwitt, Shibai Li, Bryce J. Katch, Michael E. O’Donnell, Shixin Liu, Xiaolan Zhaomutations (see text) was exchanged with the wild-type Smc6 gene using the Gibson Assembly Cloning Kit (NEB E5510S). All plasmids were sequenced to confirm correct construction. Expression and purification of both types

(15) Fusobacterium nucleatum Enhances Intestinal Adaptation of Vibrio cholerae via Interspecies Biofilm FormationMicroorganismsJanuary 16, 2026Guozhong Chen, Jiamin Chen, Xiangfeng Wang, Dingming Guo, Zhi LiuWaltham, MA, USA), and the linearized vector was assembled using Gibson Assembly® Cloning Kit (Cat: E5510S, New England Biolabs (Beijing) Ltd., Beijing, China) according to the manufacturer’s instructions. The

(16) The ubiquitin ligase KLHL6 drives resistance to CD8+ T cell dysfunctionNatureJanuary 14, 2026Hongcheng Cheng, Yapeng Su, Xiaoli Pan, Yue Xu, Ermei Xie, Jing Du, Daniel G. Chen, Xiaomeng Dai, Raphael Gottardo, Philip D. Greenberg, et al.amplified through PCR and cloned into BbsI-digested pSL21-mCherry vector using Gibson Assembly Kit (NEB, E5510S). The product of Gibson Assembly reaction was then introduced into TG1 Electrocompetent Cells (Biosearch

(17) Microbiota utilization of intestinal amino acids modulates cancer progression and anticancer immunityCell Host & MicrobeJanuary 14, 2026Shanshan Qiao, Ting-Ting Li, Mingeum Jeong, Chuanfa Liu, Sayaka Mizutani, Sung-Min Hwang, Yaxin Li, Mengze Lyu, Kazuhiro Nishiyama, Yu-Ang Tang, et al.3446-005-01 DNA Clean and Concentrator,Zymo Research,Cat # D4003 Gibson Assembly Cloning Kit,NEB,Cat # E5510S Instant Sticky-end Ligase,NEB,Cat # M0370S Plasmid Midiprep Kit,Zymo Research,Cat # D4201 PrimeScript™

(18) The mechanism of cell-cycle-dependent proteasomal degradation of archaeal ESCRT-III homolog CdvB in SulfolobusThe EMBO JournalJanuary 9, 2026Yin-Wei Kuo, Jovan Traparić, Sherman Foo, Buzz Baumbackbone at both 5’ and 3’ ends. Fragments were assembled using Gibson Assembly (New England Biolabs, E5510S) in pSVAara-FX-stop plasmid double-digested with NcoI and XhoI. Similarly, cloning of saci_0800-HA overexpression

(19) Modelling EWS::FLI1 protein fluctuations reveal determinants of tumor plasticity in Ewing sarcomaEMBO Molecular MedicineJanuary 3, 2026Veveeyan Suresh, Christoph Hafemeister, Andri Konstantinou, Sarah Grissenberger, Caterina Sturtzel, Martha M Zylka, Florencia Cidre-Aranaz, Andrea Wenninger-Weinzierl, Karla Queiroz, Dorota Kurek, et al.EcoRV-digested pUC57 plasmid backbone (Genscript, Cat#SD1176) using the Gibson Assembly® Cloning Kit (NEB, Cat#E5510S). For in vivo mouse experiments, Luc2 expressing dual marker selection vector (pRP[Exp]-EGFP/Hygro-CAG>Luc2,

(20) The role of kinase domain dimerization in EGFR activationStructureDecember 19, 2025Zaritza O. Petrova, Long Han, Yuko Tsutsui, Joshua B. Sheetz, Kumar D. Ashtekar, Mark A. LemmonE0554S QuikChange Site Directed Mutagenesis Kit,Agilent,Cat#: 200519 Gibson Assembly® Cloning Kit,NEB,Cat#: E5510S Deposited data,Deposited data,Deposited data KDDICRΔC cryo-EM structure,This study,PDB: 9P9U KDDICRΔC

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Gibson Assembly Cloning Kit

Gibson Assembly Cloning Kit from New England Biolabs

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Gibson Assembly Cloning Kit from New England Biolabs

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