Gibson Assembly Cloning Kit E5510S from New England Biolabs

Product Specs
ItemGibson Assembly Cloning Kit
CompanyNew England Biolabs
Price $207.00 Inquire
Catalog NumberE5510S
Quantity10 reactions
ApplicationsDNA Manipulation
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Endocytic motif on HIV-1 Env regulates cleavage status and antibody neutralization of cell-free and cell-to-cell infectionJournal of VirologyJune 23, 2026Dania M. Figueroa Acosta, Tony R. Valencia, Hongru Li, Benjamin K. Chenand XhoI-HF prior to insertion of mutant Env sequences by Gibson assembly (New England BioLabs, Cat. E5510S). All constructs were verified by Sanger sequencing across all amplified regions. Cell culture 293T cells

(2) Human XIRP1 is a new podosome protein targeting cytosolic bacteria as part of the IFN-γ defense programJ ImmunolJune 15, 2026Rodolfo Urbano, Alexander S Low, Aglaia Ntokou, Eui-Soon Park, Kyle Tretina, Alexandru Tunaru, Ryan G Gaudet, John D MacMickingwere cloned into HpaI-digested pMSCVpuro using the Gibson Assembly Cloning Kit (New England Biolabs, E5510S). Plasmids were transformed into E. coli NEB 5-alpha and the purified plasmids verified by Sanger sequencing

(3) Engineering novel AAV capsids by broadly attenuated and subsequent muscle-specific tropism in mice and NHPsMolecular Therapy AdvancesJune 11, 2026Yue Pan, Yujian Zhong, Huan Chen, Youwei Zhang, Zhiyong Dai, Junlin Chen, Keqin Tan, Xiaoqu Chen, Danlan Qiu, Longxiang Sheng, et al.AAV.Zero1, AAV.Zero2, and AAV.Zero3 were constructed by Gibson assembly (New England Biolabs [NEB], E5510S). The ∼5,000 bp plasmid backbone was recovered from restriction digestion of SmiI and BshTI of pAAV2-RC.

(4) Genetic diversity and regulatory features of human-specific NOTCH2NL duplicationsCell GenomicsJune 10, 2026Taylor D. Real, Prajna Hebbar, DongAhn Yoo, Francesca Antonacci, Ivana Pačar, Danilo Dubocanin, Mark Diekhans, Gregory J. Mikol, Oyeronke G. Popoola, Benjamin J. Mallory, et al.commercial assays ,, PacBio MAS-seq Kinnex kit,PacBio,PN: 103-072-000 Gibson Assembly Cloning kit,NEB,Cat#E5510S High Efficiency Transformation Protocol,NEB,Cat#C2987H Monarch Plasmid Miniprep kit,NEB,Cat#T10104 ,, Deposited

(5) D-alanine aminotransferase (Dat) promotes Staphylococcus aureus colonization fitness on human nasal respiratory epitheliumbioRxivJune 2, 2026Andrea I. Boyd, Katia A. Quintanilla, Isabel F. Escapa, Millie A. Lewis, Leah A. Kafer, Xi-Lei Zeng, Sarah E. Blutt, Carolyn B. Ibberson, Katherine P. Lemonwas linearized with SmaI (NEB R0141S) following the manufacturer’s instructions. Gibson Assembly (NEB E5510S) was performed using a 5-fold molar excess of each fragment relative to the linearized pJB38. Assembly

(6) CRISPR activation of endogenous PKD1 increases polycystin-1 levels and suppresses cellular features of ADPKDbioRxivMay 27, 2026Anubhav Chakraborty, Meekha M. Varghese, Darren P. Wallace, Christopher J. Ward, Alan S.L. YuLenti-GG-hUbc-dsRED plasmid (gift from Charles Gersbach; Addgene plasmid, 84034) using Gibson assembly (NEB, E5510S) to create individual mouse lentiviral sgRNA transfer plasmids (pLV-Pkd1-sgRNA-hUbc-dsRED) that were

(7) An RNF4-Based Tool for Tracking Subcellular Localization of PolySUMOylation During Cellular StressBiomoleculesMay 20, 2026Joseph S. Floramo, Yaguang Zhao, Lorna Cohen, Kristin Gallik, David Brass, Tao Yang(University of Dundee). Inserts were cloned into pCMV-eGFP-C3 vectors using a Gibson Assembly kit (Lot#E5510S, New England Biolabs, Inc., Ipswich, MA, USA). For viral production, GFP-tagged WT or MT RNF4 polySIM

(8) Microenvironmental arginine restriction sensitizes pancreatic cancers to polyunsaturated fatty acids by suppression of lipid synthesisbioRxivMay 4, 2026Patrick B. Jonker, Mumina Sadullozoda, Guillaume Cognet, Juan J. Apiz Saab, Kelly H. Sokol, Violet X. Wu, Deepa Kumari, Colin Sheehan, Mete E. Ozgurses, Darby Agovino, et al.the primer sequences. These fragments were then assembled using HiFi Gibson assembly master mix (NEB E5510S). The sequence of the resulting vector was confirmed sequencing (Plasmidsaurus). HEK293T cells (Dharmacon)

(9) Prothrombinase processivity is conferred by substrate allosteryThe EMBO JournalApril 22, 2026Fatma Işık Üstok, Alexandre Faille, Alan J Warren, James A Huntingtonreagents","Chemicals, enzymes and other reagents" Gibson Assembly Cloning Kit,New England Biolabs,Cat♯ E5510S Q5 Hot Start High Fidelity 2X Master Mix,New England Biolabs,Cat♯ M0494S "QIAprep Spin, miniprep kit",QIAGEN,Cat♯

(10) hnRNPUL1 has a dead polynucleotide kinase domain that regulates RNA and protein interactionsiScienceApril 17, 2026Carmen V. Apostol, Ang Li, Peter Daniels, Llywelyn Griffith, Johnathan Cooper-Knock, Elisa Aguilar-Martinez, Ivaylo D. Yonchev, Ashleigh G.R. Whelan, Pamela J. Shaw, Ian M. Sudbery, et al.commercial assays,Critical commercial assays ,, Gibson assembly cloning kit,New England Biolabs,Cat# E5510S Q5 Site-Directed Mutagenesis Kit,New England Biolabs,Cat# E0554S ,, Deposited data,Deposited data,Deposited

(11) Heparan sulfate is essential for Drosophila FGF exportbioRxivApril 6, 2026Guilherme Oliveira Barbosa, Christian Solis-Calero, Thomas B. KornbergC-terminal to the FGF domain of Bnl (Sohr et al., 2019), using Gibson Assembly Cloning Kit (NEB Cat#: E5510S), Q5® High-Fidelity DNA Polymerase (NEB #M0491) and pAc5.1/V5-His A linearized by XhoI digestion. Based

(12) Exonic enhancers are a widespread class of dual-function regulatory elementsNature CommunicationsApril 2, 2026Jean-Christophe Mouren, Magali Torres, Antoinette van Ouwerkerk, Iris Manosalva, Frederic Gallardo, Salvatore Spicuglia, Benoit Ballesterusing the Nucleospin Gel Cleanup kit (Macherey-Nagel, cat. #740609.50). For Gibson Assembly (NEB, cat. #E5510S), 150 ng of digested vector and 40 ng of the purified PCR_A product were incubated in two separate 20

(13) Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cellsMolecular Therapy OncologyMarch 19, 2026Juliane Mietz, Lukas Egli, Meike Misiune, Arianna Picozzi, Nicolò Coianiz, Danielle Thompson, Marianna Ponzo, Alisson Charmey, Beat Bornhauser, Chiara F. Magnani, et al.(New England Biolabs, Cat. R3101)-linearized pUC19 (Addgene, Cat. #50005) by Gibson Assembly (New England Biolabs, Cat. E5510S) and introduced into competent NEB5alpha cells (New England Biolabs, Cat. C2987) by heat shock. AAV6

(14) RSRC2 is a novel RNA-binding protein that safeguards mitotic fidelity by interacting with the lncRNA C1QTNF1-AS1Nucleic Acids ResMarch 17, 2026Parnia Babaei, Alice O Coomer, Kaliya Georgieva, Giulia Guiducci, Elisa Vitiello, Martin Dodel, Eleni Maniati, Hanya Elsayed Eid, Anisha Thind, Sneha Krishnamurthy, et al.cloned upstream of the MS2 stem-loop sequence of the 10xMS2 vector using a Gibson assembly cloning kit (E5510S, NEB) at 50°C for 15 min. The assembled C1QTNF1–AS1–10xMS2 vector was transformed into competent Escherichia coli

(15) Integrating natural and engineered genetic variations to decode regulatory influence on blood traitsCell ReportsFebruary 24, 2026Manuel Tardaguila, Dominique Von Schiller, Michela Colombo, Ilaria Gori, Eve L. Coomber, Thomas Vanderstichele, Paola Benaglio, Chiara Chiereghin, Sebastian Gerety, Dragana Vuckovic, et al.Buffer,Roche,Cat#KK5516 pGEM T easy system,Promega,Cat#A1360 Gibson Assembly® Cloning Kit,New England Biolabs,Cat#E5510S KAPA Illumina SYBR Universal Lib Q. Kit,Roche,Cat#07960140001 MiSeq Reagent Kit v2 300 cycle,Illumina,Cat#MS-102-2002 Qubit

(16) Membrane-associated periodic skeleton regulates major forms of endocytosis in neurons through a signaling-driven positive feedback loopSci AdvFebruary 13, 2026Jinyu Fei, Yuanmin Zheng, Caden LaLonde, Yuan Tao, Ruobo Zhouexpression vector FUGW (Addgene, plasmid #14883), through Gibson assembly reaction (New England Biolabs, E5510S) according to the manufacturer’s instructions. Coding sequences for CAV1, FLOT1, SH3GL1 (synthesized

(17) PHLDA1 is a shared diagnostic and key mediator of inflammatory fibrosis in heart and kidneyFrontiers in ImmunologyFebruary 5, 2026Lei Hua, Liangru Shen, Yongshou Tao, Chentong Wang, Xiaohang Shaosubsequent experiments, both cell types were immortalized through lentiviral transduction of SV40LT (E5510S, New England Biolabs) and following our previous protocol (21). Immortalized cell types were cultured

(18) Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epibolyDevelopmentFebruary 1, 2026Haoyu Wan, Sifa Quibria, Ernest Iu, Sirma Damla User, Sergey V. Plotnikov, Ashley E. E. BrucepFP2 using the Gibson assembly method (Gibson et al., 2009) with the Gibson Assembly Cloning Kit (NEB, E5510S). For yolk-specific expression of full-length Camsap2a, the plasmid pzTol2[Exp]-{wnt8 promoter}>{camsap2a}

(19) Tunable Chemical and Optical Control of ER-Plasma Membrane Contact Site Geometry and Dynamics with High-Fidelity VisualizationbioRxivJanuary 31, 2026Shaoqing Zhang, Jinyu Fei, Yuanmin Zheng, Ruobo Zhou(New England Biolabs, M0491L), and plasmids were assembled using Gibson assembly (New England Biolabs, E5510S) according to the manufacturer’s instructions. Unless otherwise noted, constructs were cloned into mammalian expression

(20) BAF complex-independent gene activation by SS18::SSXbioRxivJanuary 31, 2026Afroditi Sotiriou, Jinxiu Li, Sanya Middha, Jake A. Ward, Selina Troester, Lianghao Mao, Martin Schneider, Dario Frey, Eliza C. Wray, Sara Bocedi, et al.plasmid. For cloning of the miniTurbo-V5-SS18::SSX2 plasmid, SS18::SSX2 was cloned by Gibson assembly (NEB, E5510S) into BamHI (NEB) digested pENTR221_miniTurbo_V546. Primers to amplify SS18::SSX2 contained Gibson compatible

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Gibson Assembly Cloning Kit

Gibson Assembly Cloning Kit from New England Biolabs

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Gibson Assembly Cloning Kit from New England Biolabs

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