Gibson Assembly Cloning Kit E5510S from New England Biolabs

Product Specs
ItemGibson Assembly Cloning Kit
CompanyNew England Biolabs
Price $207.00 Inquire
Catalog NumberE5510S
Quantity10 reactions
ApplicationsDNA Manipulation
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Microenvironmental arginine restriction sensitizes pancreatic cancers to polyunsaturated fatty acids by suppression of lipid synthesisElifeAugust 5, 2026Patrick B Jonker, Mumina Sadullozoda, Guillaume Cognet, Juan J Apiz Saab, Kelly H Sokol, Violet X Wu, Deepa Kumari, Colin Sheehan, Mete E Ozgurses, Darby Agovino, et al.the primer sequences. These fragments were then assembled using HiFi Gibson assembly master mix (NEB E5510S). The sequence of the resulting vector was confirmed sequencing (Plasmidsaurus). HEK293T cells (Dharmacon)

(2) Decoding strain level interactions between Vibrio cholerae and gut microbiotaVirulenceJuly 30, 2026Guozhong Chen, Shucheng Li, Qinghua Zhao, Yanfei Han, Zhiyuan Zhang, Zhi Liulinearized vector was assembled using Gibson Assembly® Cloning Kit (New England Biolabs (Beijing) Ltd., Cat# E5510S) according to the manufacturer’s instructions. The resulting construct was transformed into E. coli

(3) Fission yeast RPA–TERT–Tpz1 TPP1 complex promotes telomere extension and suppresses telomere recombinationbioRxivJuly 28, 2026Bettina A. Moser, Madeline Points, Sourav Agrawal, Adam C. Didier, Amanda K. Mennie, Ci Ji Lim, Yong-jie Xu, Toru M. Nakamuragenotypes. Point mutations and truncations were generated with Q5 PCR (NEB, M0491L), Gibson Assembly (NEB, E5510S), or site-directed mutagenesis kits Q5 (NEB, E0554S) or QuikChange Lightning (Agilent, 210519) on plasmids

(4) Multi-subunit collaboration enables Smc5/6 to function as a composite SUMO E3 complexNature CommunicationsJuly 23, 2026Xiaoyu Xue, Jiayi Fan, Shibai Li, Sofya Ignatyeva, Patricia Gallegos-Elias, Heather McEntire-Benitez, Xinji Zhu, Tanu Rani Kar, John Epps, Lillian Eliaz, et al.was exchanged with the corresponding wild-type gene using the NEB Gibson-Assembly Cloning Kit (NEB E5510S). All plasmids were confirmed by sequencing. Purification of core-Smc5/6 complex Expression and purification

(5) Toxoplasma TgATG2 works with TgATG9 and TgProp1 to drive autophagic flux for parasite extracellular survival and persistencebioRxivJuly 19, 2026Silvia Masci, Pariyamon Thaprawat, Federica Piro, Tracey L. Schultz, Vern B. Carruthers, Manlio Di CristinaP30/P31. The backbone and insert fragments were joined using Gibson Assembly (New England Biolabs, E5510S) prior to bacterial transformation. The CAT resistance was then replaced with the Tub/DHFR resistance

(6) A pooled image-based CRISPR screen identifies EAF1 as a T. gondii modulator of ESCRT subversionbioRxivJuly 9, 2026Einar B. Olafsson, Christophe-Sebastien Arnold, Jacob A. Kellermeier, Patrick A. Rimple, Hargobinder Kaur, Yifan Wang, Jonathan Z. Sexton, Staffan Svärd, Vern B. Carruthers, Matthew J. O'Mearastrain generation To generate RHCas9DsRed, we first generated pDsRedHXGPRT by Gibson assembly (NEB E5510S) using primers 1-4 (Supplementary Table 14) bridging the chloramphenicol coding region of pDsRedCat25

(7) ARHGAP18 complexes with both YAP and Merlin and is required for basal actin bundlesMol Biol CellJune 30, 2026Emma C. Murray, Gillian M. Hodge, Khanh Pham, Shane Fraher, Leighton S. Lee, Cameron A.R. Mitchell, Namya Manoj, Christine E. Schaner Tooley, Yongho Bae, Andrew T. Lombardocloned into the mammalian expression vector PQCXIP using NEB Gibson Assembly cloning Kit (Catalogue # E5510S). Transformations were done into OneShot TOP10 bacteria (Thermo Fisher; Catalogue # C404010), selected

(8) Endocytic motif on HIV-1 Env regulates cleavage status and antibody neutralization of cell-free and cell-to-cell infectionJournal of VirologyJune 23, 2026Dania M. Figueroa Acosta, Tony R. Valencia, Hongru Li, Benjamin K. Chenand XhoI-HF prior to insertion of mutant Env sequences by Gibson assembly (New England BioLabs, Cat. E5510S). All constructs were verified by Sanger sequencing across all amplified regions. Cell culture 293T cells

(9) Multiplexed cytokine and antigen mRNA administration generates durable anti-tumor immunity against pancreatic cancerNature CommunicationsJune 19, 2026Chaitanya N. Parikh, Kelly D. DeMarco, Nikita Bhalerao, Hadiya K. Giwa, Griffin I. Kane, Ronnie W. Dinnell, Boyang Ma, Haruka Mori, Meghan L. Brassil, Katherine C. Murphy, et al.promoter, 5’ UTR, 3’ UTR, and 110 bp poly A tail using Gibson Assembly (New England Biolabs; Cat. No. E5510S) according to the manufacturer’s protocol. Sequences for the pUC19 vector backbone and cloned cytokine

(10) Human XIRP1 is a new podosome protein targeting cytosolic bacteria as part of the IFN-γ defense programJ ImmunolJune 15, 2026Rodolfo Urbano, Alexander S Low, Aglaia Ntokou, Eui-Soon Park, Kyle Tretina, Alexandru Tunaru, Ryan G Gaudet, John D MacMickingwere cloned into HpaI-digested pMSCVpuro using the Gibson Assembly Cloning Kit (New England Biolabs, E5510S). Plasmids were transformed into E. coli NEB 5-alpha and the purified plasmids verified by Sanger sequencing

(11) Deciphering small sequence differences in T cell receptor-antigen pairingNature CommunicationsJune 13, 2026Yi Han, Yuqiu Yang, James Zhu, Farjana J. Fattah, Mitchell S. von Itzstein, Minying Zhang, Casey Bermack, Peixin Jiang, Shailbala Singh, Yanhua Tian, et al.assembly reaction was carried out using the Gibson assembly kit purchased from New England Biolabs (E5510S) according to the provided protocols. HEK293T cells were transiently transfected with 1 µg each of CD3

(12) Engineering novel AAV capsids by broadly attenuated and subsequent muscle-specific tropism in mice and NHPsMolecular Therapy AdvancesJune 11, 2026Yue Pan, Yujian Zhong, Huan Chen, Youwei Zhang, Zhiyong Dai, Junlin Chen, Keqin Tan, Xiaoqu Chen, Danlan Qiu, Longxiang Sheng, et al.AAV.Zero1, AAV.Zero2, and AAV.Zero3 were constructed by Gibson assembly (New England Biolabs [NEB], E5510S). The ∼5,000 bp plasmid backbone was recovered from restriction digestion of SmiI and BshTI of pAAV2-RC.

(13) Genetic diversity and regulatory features of human-specific NOTCH2NL duplicationsCell GenomicsJune 10, 2026Taylor D. Real, Prajna Hebbar, DongAhn Yoo, Francesca Antonacci, Ivana Pačar, Danilo Dubocanin, Mark Diekhans, Gregory J. Mikol, Oyeronke G. Popoola, Benjamin J. Mallory, et al.commercial assays ,, PacBio MAS-seq Kinnex kit,PacBio,PN: 103-072-000 Gibson Assembly Cloning kit,NEB,Cat#E5510S High Efficiency Transformation Protocol,NEB,Cat#C2987H Monarch Plasmid Miniprep kit,NEB,Cat#T10104 ,, Deposited

(14) D-alanine aminotransferase (Dat) promotes Staphylococcus aureus colonization fitness on human nasal respiratory epitheliumbioRxivJune 2, 2026Andrea I. Boyd, Katia A. Quintanilla, Isabel F. Escapa, Millie A. Lewis, Leah A. Kafer, Xi-Lei Zeng, Sarah E. Blutt, Carolyn B. Ibberson, Katherine P. Lemonwas linearized with SmaI (NEB R0141S) following the manufacturer’s instructions. Gibson Assembly (NEB E5510S) was performed using a 5-fold molar excess of each fragment relative to the linearized pJB38. Assembly

(15) 3’UTR regulation of axon translation and optic nerve regenerationMolecular NeurodegenerationMay 29, 2026Minjuan Bian, Emma L. Huie, Xin Xia, Michael Nahmou, Savana A. Maxfield, Liang Li, Liang Liu, Ziming Luo, Christina Tsien, Katherine J. Healzer, et al.synthesized and cloned directly into pmyrDendra backbone using Gibson Assembly (New England Biolabs, E5510S). The coding region of mouse Src with constitutive active mutation in the kinase domain (Y527F) followed

(16) RACK1 governs a dual metabolic switch in lung adenocarcinoma through c-Src/G6PD and TRIM21/LDHA AxesCell Death & DiseaseMay 29, 2026Huimin Wan, Chao Li, Mengqian Xia, Lin Dong, Tingting Lin, Weiwei Guo, Fanglei Jiao, Jingjing Lu, Zhongliang GuoExtraction Kit (Tiangen, DP103). Ligation was performed using the Gibson Assembly Master Mix (NEB, #E5510S). The assembled products were transformed into DH5α competent cells (Vazyme, C502) and plated on LB

(17) CRISPR activation of endogenous PKD1 increases polycystin-1 levels and suppresses cellular features of ADPKDbioRxivMay 27, 2026Anubhav Chakraborty, Meekha M. Varghese, Darren P. Wallace, Christopher J. Ward, Alan S.L. YuLenti-GG-hUbc-dsRED plasmid (gift from Charles Gersbach; Addgene plasmid, 84034) using Gibson assembly (NEB, E5510S) to create individual mouse lentiviral sgRNA transfer plasmids (pLV-Pkd1-sgRNA-hUbc-dsRED) that were

(18) Unmasking pathogen traits for chronic colonization in neurogenic bladderCell ReportsMay 26, 2026Seth A. Reasoner, Brendan T. Frainey, Owen F. Hale, Alexandra Borden, M. Kyle Graham, Elise Turner, Lucas R. Brenes, Carl B.W. Soderstrom, Hamilton Green, Jonathan E. Schmitz, et al.VITEK® MS PRIME,bioMérieux,N/A Electroporation Cuvettes,BioRad,Cat # 1652089 Gibson Assembly mix,New England Biolabs,Cat #E5510S Silver Stain Kit,Pierce/Thermo Scientific,Cat # 24612 Deposited data,Deposited data,Deposited data 16S

(19) PKR condensation at viral replication complexes initiates its activationCell reportsMay 26, 2026Blomqvist EK, Bracci N, Winstone H, Watkins JM, Weiss SR, Burke JM.Bioscience,NU-1619-633 Critical commercial assays,Critical commercial assays,Critical commercial assays Gibson Assembly Kit,NEB,E5510S Duolink® Proximity Ligation Assay In Situ (Mouse/Rabbit),Millipore Sigma,DUO92101-1KT QuikChange XL

(20) An RNF4-Based Tool for Tracking Subcellular Localization of PolySUMOylation During Cellular StressBiomoleculesMay 20, 2026Joseph S. Floramo, Yaguang Zhao, Lorna Cohen, Kristin Gallik, David Brass, Tao Yang(University of Dundee). Inserts were cloned into pCMV-eGFP-C3 vectors using a Gibson Assembly kit (Lot#E5510S, New England Biolabs, Inc., Ipswich, MA, USA). For viral production, GFP-tagged WT or MT RNF4 polySIM

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Gibson Assembly Cloning Kit

Gibson Assembly Cloning Kit from New England Biolabs

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Gibson Assembly Cloning Kit from New England Biolabs

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