ONE-Glo Luciferase Assay System E6130 from Promega

Product Specs
ItemONE-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6130
Quantity1L
Sample TypeMammalian cells
MethodONE-Glo Assay System
Detection TargetLuciferase

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A ligand-mimetic anti-TREM2 agonist antibody elevates soluble TREM2 and ameliorates pathology in mouse models of Alzheimer’s disease and multiple sclerosisJournal of NeuroinflammationFebruary 23, 2026Buxin Chen, Hsueh-Chung Lu, Lei Huang, Jean Wang, Matthew Bujold, Lingyun Tang, Xin Du, Yubin Wangincubation, luciferase activity was measured using Promega ONE-Glo™ Luciferase Assay System (Promega, #E6130) according to the manufacturer’s protocol. Hybridomas secreting TREM2-specific IgG with agonistic activity

(2) Macroalgae-Inspired Brominated Chalcones as Cosmetic Ingredients with the Potential to Target Skin InflammagingMar DrugsJuly 2, 2025Ana Jesus, Sara Gimondi, Sónia A. Pinho, Helena Ferreira, Nuno M. Neves, Andreia Palmeira, Emília Sousa, Isabel F. Almeida, Maria T. Cruz, Honorina Cidade96-well plates were washed with 1× PBS. An equal volume of 1× PBS and lysis buffer (One-GLO Luciferase, cat. no. E6130, Promega, Madison, WI, USA) was added to the wells, and the plates were incubated for 20 min at room

(3) Structural insights into CXCR4 modulation and oligomerizationNature structural & molecular biologyFebruary 1, 2025Saotome K, McGoldrick LL, Ho JH, Ramlall TF, Shah S, Moore MJ, Kim JH, Leidich R, Olson WC, Franklin MC.CO2. At the conclusion of the incubations, the luciferase activity was detected using OneGlo (Promega, E6130) and luminescence was recorded by an EnVision Plate reader using EnVision Manager version 1.14 (Perkin

(4) Structures of complete extracellular assemblies of type I and type II Oncostatin M receptor complexesNature communicationsNovember 12, 2024Zhou Y, Stevis PE, Cao J, Ehrlich G, Jones J, Rafique A, Sleeman MW, Olson WC, Franklin MC.1:3 dilution series overnight. To measure luciferase activity, an equal volume of OneGlo (Promega, #E6130) was added to each well, followed by placement of a white adhesive bottom seal (PerkinElmer/Revvity,

(5) A PD-1-targeted, receptor-masked IL-2 immunocytokine that engages IL-2Rα strengthens T cell-mediated anti-tumor therapiesCell Reports MedicineOctober 15, 2024Jiaxi Wu, Nicolin Bloch, Aaron Y. Chang, Ramandeep Bhavsar, Qingqing Wang, Alison Crawford, David J. DiLillo, Kristin Vazzana, Katja Mohrs, Drew Dudgeon, et al."Tumor Dissociation Kit, mouse",Miltenyi Biotec,Cat# 130-096-730 ONE-Glo™ Luciferase Assay System,Promega,Cat# E6130 EasySep™ Direct Human PBMC Isolation Kit,STEMCELL Technologies,Cat# 19654 EasySep™ Human T cell Isolation Kit

(6) No Evidence of SARS‐CoV‐2 Infection in Urban Wildlife of Hokkaido, JapanTransboundary and Emerging DiseasesAugust 13, 2024Anastasiia Kovba, Naganori Nao, Michito Shimozuru, Mariko Sashika, Chihiro Takahata, Kei Sato, Keiya Uriu, Masami Yamanaka, Masanao Nakanishi, Genta Ito, et al.days postinfection, infected cells were lysed with a One-Glo luciferase assay system (Promega, Cat# E6130), a Bright-Glo luciferase assay system (Promega, Cat# E2650), or a britelite plus Reporter Gene Assay

(7) AS03 adjuvant enhances the magnitude, persistence, and clonal breadth of memory B cell responses to a plant-based COVID-19 vaccine in humansScience ImmunologyApril 5, 2024Lilit Grigoryan, Yupeng Feng, Lorenza Bellusci, Lilin Lai, Bushra Wali, Madison Ellis, Meng Yuan, Prabhu S. Arunachalam, Mengyun Hu, Sangeeta Kowli, et al.hours later and luciferase activity was measured using One-Glo luciferase assay system (Promega, Cat# E6130). Virus-only and cells-only controls were used in these assays. The IC50 titers were calculated using

(8) Factors Associated with Neutralizing Antibody Responses following 2-Dose and 3rd Booster Monovalent COVID-19 Vaccination in Japanese People Living with HIVVirusesApril 2, 2024Isaac Ngare, Toong Seng Tan, Mako Toyoda, Takeo Kuwata, Soichiro Takahama, Eriko Nakashima, Naoya Yamasaki, Chihiro Motozono, Teruhisa Fujii, Rumi Minami, et al.density of 22,000 cells/well and incubated for 48 h. A luciferase substrate (ONE-Glo, Promega, cat # E6130, Madison, MI, USA) equipped with a lysis agent was added to the culture supernatant in accordance with

(9) Single-cell analysis of immune recognition in chronic myeloid leukemia patients following tyrosine kinase inhibitor discontinuationLeukemiaJanuary 1, 2024Huuhtanen J, Adnan-Awad S, Theodoropoulos J, Forstén S, Warfvinge R, Dufva O, Bouhlal J, Dhapola P, Duàn H, Laajala E, et al.measured with Novocyte Quanteon. Viability assay for co-culture ONE-Glo™ Luciferase assay (Promega, E6130) was performed to monitor cell viability after co-culture. Co-culture plates without GolgiStop and anti-CD107a

(10) VSIG4 interaction with heparan sulfates inhibits VSIG4-complement bindingGlycobiologyAugust 14, 2023Ebstein SY, Rafique A, Zhou Y, Krasco A, Montalvo-Ortiz W, Yu L, Custodio L, Adam RC, Bloch N, Lee K, et al.C3b/iC3b and a dose response of heparin or HS at 37°C for 5.5 h. One-glo luciferase assay buffer (Promega #E6130) was then added to wells and luciferase was imaged on an Envision. Where indicated, Jurkat cells were

(11) Virological characteristics of the SARS-CoV-2 XBB variant derived from recombination of two Omicron subvariantsNature communicationsMay 16, 2023Tamura T, Ito J, Uriu K, Zahradnik J, Kida I, Anraku Y, Nasser H, Shofa M, Oda Y, Lytras S, et al.plate. At 2 d.p.i., the infected cells were lysed with a One-Glo luciferase assay system (Promega, Cat# E6130), a Bright-Glo luciferase assay system (Promega, Cat# E2650), or a britelite plus Reporter Gene Assay

(12) Convergent evolution of SARS-CoV-2 Omicron subvariants leading to the emergence of BQ.1.1 variantNature communicationsMay 11, 2023Ito J, Suzuki R, Uriu K, Itakura Y, Zahradnik J, Kimura KT, Deguchi S, Wang L, Lytras S, Tamura T, et al.plate. At 2 d.p.i., the infected cells were lysed with a One-Glo luciferase assay system (Promega, Cat# E6130), a Bright-Glo luciferase assay system (Promega, Cat# E2650), or a britelite plus Reporter Gene Assay

(13) Systems biological assessment of the temporal dynamics of immunity to a viral infection in the first weeks and months of lifemedRxivFebruary 3, 2023Florian Wimmers, Allison R. Burrell, Yupeng Feng, Hong Zheng, Prabhu S. Arunachalam, Mengyun Hu, Sara Spranger, Lindsay Nyhoff, Devyani Joshi, Meera Trisal, et al.h later, and luciferase activity was measured using One-Glo luciferase assay system (Promega, Cat# E6130). The assay of each sample was performed in duplicate, and the 50% neutralization titer was calculated

(14) The SARS-CoV-2 spike S375F mutation characterizes the Omicron BA.1 variantiScienceDecember 22, 2022Kimura I, Yamasoba D, Nasser H, Zahradnik J, Kosugi Y, Wu J, Nagata K, Uriu K, Tanaka YL, Ito J, et al.Bright-Glo Luciferase Assay System (Promega, cat# E2620) or a One-Glo luciferase assay system (Promega, cat# E6130) and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega)

(15) Monoclonal antibodies against S2 subunit of spike protein exhibit broad reactivity toward SARS-CoV-2 variantsJournal of biomedical scienceDecember 22, 2022Ko SH, Chen WY, Su SC, Lin HT, Ke FY, Liang KH, Hsu FF, Kumari M, Fu CY, Wu HC.medium was replaced with DMEM containing 10% FBS for 48 h. The ONE-Glo™ luciferase substrate (Promega; E6130) was used to generate luminescence signal (relative light units, RLU) that was detected with a spectrophotometer

(16) Screening Method for the Identification of Compounds That Activate Pregnane X ReceptorCurrent ProtocolsDecember 5, 2022Caitlin Lynch, Srilatha Sakamuru, Menghang Xia(Millipore Sigma, cat. No. 294136, CAS Number 14866-33-2) ONE-Glo Luciferase Assay System (Promega, cat. No. E6130) Biological Safety Cabinet (Labconco, cat. No. 302610101 or equivalent) Vacuum filter system, 500 mL

(17) Grainyhead 1 acts as a drug-inducible conserved transcriptional regulator linked to insulin signaling and lifespanNature CommunicationsDecember 1, 2022Giovanna Grigolon et al.CLARIOstar microplate reader (BMG LABTECH) and the ONE-Glo Luciferase Assay System (Promega Corporation, #E6130) according to the manufacturers’ instructions. Details for individual assay setups are as follows: Primary

(18) Virological characteristics of the SARS-CoV-2 Omicron BA.2.75 variantCell Host & MicrobeNovember 9, 2022Akatsuki Saito, Tomokazu Tamura, Jiri Zahradnik, Sayaka Deguchi, Koshiro Tabata, Yuki Anraku, Izumi Kimura, Jumpei Ito, Daichi Yamasoba, Hesham Nasser, et al.plate. At 2 d.p.i., the infected cells were lysed with a One-Glo luciferase assay system (Promega, Cat# E6130) or a Bright-Glo luciferase assay system (Promega, Cat# E2650), and the luminescent signal was measured

(19) Comprehensive profiling of the human viral exposome in households containing an at-risk child with mitochondrial disease during the 2020-2021 COVID-19 pandemicClin Transl MedNovember 6, 2022Eliza M. Gordon-Lipkin, Christopher S. Marcum, Shannon Kruk, Elizabeth Thompson, Sophie E. M. Kelly, Heather Kalish, Lorenza Bellusci, Surender Khurana, Kaitlyn Sadtler, Peter J. McGuireh later, and luciferase activity was measured using ONE‐Glo luciferase assay system (Promega, Cat# E6130). The assay of each sample was performed in duplicate, and the 50% neutralization titre was calculated

(20) Dissecting Naturally Arising Amino Acid Substitutions at Position L452 of SARS-CoV-2 SpikeJournal of virologyOctober 26, 2022Tan TS, Toyoda M, Ode H, Barabona G, Hamana H, Kitamatsu M, Kishi H, Motozono C, Iwatani Y, Ueno T.later by measuring the luciferase activity with a One-Glo luciferase assay system (Promega, catalog no. E6130) in a Centro XS3 LB960 luminometer (Berthold Technologies). Data were normalized to WT D614G (5 ng)

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ONE-Glo Luciferase Assay System

ONE-Glo Luciferase Assay System from Promega

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ONE-Glo Luciferase Assay System from Promega

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