Universal Kinase Activity Kit, 2 Plate EA004 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemUniversal Kinase Activity Kit, 2 Plate
CompanyR&D Systems, a Bio-Techne Brand
Price $673.00Supplier Page View Company Product Page
Catalog NumberEA004
Quantity1 Kit (2 Plates)
ReactivityMulti-Species
StorageStore the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date

Specifications/Features

Available conjugates: Sizes Available: 1 Kit (2 Plates)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) An anti-adhesive compound modulating the production of Staphylococcus aureus cell wall-anchored proteinsNature CommunicationsApril 15, 2026Allison C. Leonard, Ruina Bao, Cindy Menjivar, Megan J. Myers, Telmo O. Paiva, Zhiyong Zheng, Kirsten A. Berry, Kenneth W. Bayles, Ronald S. Flannagan, Yves F. Dufrêne, et al.performed as described previously54 with minor modifications. The Universal Kinase Kit (R&D Systems, Cat #: EA004) was used following the adjusted manufacturer’s instructions. The substrate mix was prepared in the

(2) DNA‐PKcs‐Driven YAP1 Phosphorylation and Nuclear Translocation: a Key Regulator of Ferroptosis in Hyperglycemia‐Induced Cardiac Dysfunction in Type 1 DiabetesAdvanced ScienceApril 25, 2025Junyan Wang, Xing Chang, Chun Li, Jing Gao, Zhijiang Guo, Haowen Zhuang, Lingjun Wang, Yusheng Huang, Wei Wang, Chao Li, et al.Biosite).[ 79 ] These assays were performed utilizing an Universal Kinase Activity Kit (Catalogue No. EA004, Bio‐Techne Corp., Minneapolis, MN, USA), strictly adhering to the manufacturer's guidelines. Molecular Docking

(3) Molecular insights into the structure and function of the Staphylococcus aureus fatty acid kinaseJournal of Biological ChemistryDecember 1, 2024Megan J. Myers, Zhen Xu, Benjamin J. Ryan, Zachary R. DeMars, Miranda J. Ridder, David K. Johnson, Christina N. Krute, Tony S. Flynn, Maithri M. Kashipathy, Kevin P. Battaile, et al.were ordered from Genscript. Kinase activity assay The Universal Kinase Kit (R and D systems. Cat #: EA004) was used following adjusted manufacturer instructions. The substrate mix was prepared in wells of a

(4) Structure-based virtual screening of natural compounds in preventing skin senescence: The role of epigallocatechin gallate in protein kinase C alpha-specific inhibition against UV-induced photoagingHeliyonNovember 15, 2024Cho CH, Sim WJ, Cho NC, Lim W, Lim TG.

(5) DNA-PKcs phosphorylates cofilin2 to induce endothelial dysfunction and microcirculatory disorder in endotoxemic cardiomyopathyResearch (Wash D C)March 26, 2024Yingzhen Du, Pingjun Zhu, Yukun Li, Jiachi Yu, Tian Xia, Xing Chang, Hang Zhu, Ruibing Li, Qingyong Heab111635) were used to perform in vitro kinase assays using an Universal Kinase Activity Kit (Cat. No EA004, Bio-Techne Corp., Minneapolis, MN, USA) according to the manufacturer’s instructions [80]. Immunofluorescence Mouse

(6) Entrectinib inhibits NLRP3 inflammasome and inflammatory diseases by directly targeting NEK7Cell Reports MedicineDecember 19, 2023Xiangyu Jin, Didi Liu, Xinru Zhou, Xianyu Luo, Qian Huang, Yi Huangwas analyzed according to the manufacturer’s instructions of the Kinase Activity Kit (R&D Systems, EA004). LPS-induced systemic inflammation Ten-week-old male C57BL/6 mice were intraperitoneally injected

(7) Blockade of DDR1/PYK2/ERK signaling suggesting SH2 superbinder as a novel autophagy inhibitor for pancreatic cancerCell Death DisDecember 9, 2023Hui Xu, Ming Tan, Guo-Qing Hou, Ya-Zhou Sang, Li Lin, Xiao-Cai Gan, Xuan Cao, An-Dong Liuand absence of SH2 TrM-(Arg)9 was measured using a Universal Kinase Activity Kit (R&D Systems, Cat. #EA004). The assay measures the inorganic phosphate that is released from ADP via substrate phosphorylation

(8) An Fgr kinase inhibitor attenuates sepsis-associated encephalopathy by ameliorating mitochondrial dysfunction, oxidative stress, and neuroinflammation via the SIRT1/PGC-1α signaling pathwayJournal of translational medicineJuly 20, 2023Liu Y, Yang H, Luo N, Fu Y, Qiu F, Pan Z, Li X, Jian W, Yang X, Xue Q, et al.

(9) ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitudeCommunications biologyJuly 28, 2022Li H, Ou J, Li Y, Xu N, Li Q, Wu P, Peng C, Tang YC, Chang HC.FLAG peptide (150 ng/μl, Sigma). Kinase assays were carried out with a Universal kinase activity kit (EA004, R&D Systems) at 1:100 CaMKII to Autocamtide-2 Peptide (sc-3029, Santa Cruz Biotechnology), or 1:4 CaMKII

(10) Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearanceNature communicationsJuly 16, 2022Wrobel L, Hill SM, Djajadikerta A, Fernandez-Estevez M, Karabiyik C, Ashkenazi A, Barratt VJ, Stamatakou E, Gunnarsson A, Rasmusson T, et al.kinase assay In vitro kinase assay was performed using the Universal Kinase Activity Kit (R&D systems, #EA004) according to the manufacturer’s instructions. A substrate mix containing purified FLAG-VPS34 (10 ng)

(11) Yeast-produced fructosamine-3-kinase retains mobility after ex vivo intravitreal injection in human and bovine eyes as determined by Fluorescence Correlation SpectroscopyInternational journal of pharmaceuticsJune 10, 2022Minnaert AK, van Schie L, Grootaert H, Himpe J, Devos S, Weyts W, De Keersmaecker H, Braeckmans K, Van Aken E, Delanghe JR, et al.

(12) Overexpression of PpSnRK1α in Tomato Increased Autophagy Activity under Low Nutrient StressInternational journal of molecular sciencesMay 13, 2022Luo J, Yu W, Xiao Y, Zhang Y, Peng F.SnRK1 activity was determined with a Universal Kinase Activity Kit (R&D Systems, Minneapolis, MN, USA, EA004) by using AMARA polypeptide as the substrate [59]. The procedure was carried out according to the manufacturer’s

(13) A negative feedback loop of TOR signaling balances growth and stress-response trade-offs in plantsCell reportsApril 5, 2022Jamsheer K M, Jindal S, Sharma M, Awasthi P, S S, Sharma M, Mannully CT, Laxmi A.

(14) Protocol for determining the regulation of lipid kinases and changes in phospholipids in vitroSTAR ProtocolsDecember 1, 2021Cansu Karabiyik, David C. Rubinszteinassays,Critical commercial assays,Critical commercial assays ,, Universal In Vitro Assay Kit,R&D Systems,#EA004 Pierce HA magnetic beads,ThermoFisher,#88836 Pierce BCA Protein Assay Kit,ThermoFisher,#23225 ,, Experimental models

(15) Investigation of the specificity and mechanism of action of the ULK1/AMPK inhibitor SBI-0206965The Biochemical journalAugust 13, 2021Ahwazi D, Neopane K, Markby GR, Kopietz F, Ovens AJ, Dall M, Hassing AS, Gräsle P, Alshuweishi Y, Treebak JT, et al.60 min. Adenosine kinase activity was measured using the Universal Kinase Activity kit (R&D System, EA004) using adenosine (MerckMilliporeSigma, #A9251) as substrate according to the manufacturer protocol. Protein

(16) A Potential Role for Fructosamine-3-Kinase in Cataract TreatmentInternational journal of molecular sciencesApril 7, 2021De Bruyne S, van Schie L, Himpe J, De Somer F, Everaert I, Derave W, Van den Broecke C, Huizing M, Bostan N, Speeckaert M, et al.

(17) Discovery of a novel small-molecule inhibitor of Fam20C that induces apoptosis and inhibits migration in triple negative breast cancerEuropean journal of medicinal chemistryJanuary 15, 2021Zhao R, Fu L, Yuan Z, Liu Y, Zhang K, Chen Y, Wang L, Sun D, Chen L, Liu B, et al.

(18) NEK9, a novel effector of IL-6/STAT3, regulates metastasis of gastric cancer by targeting ARHGEF2 phosphorylationTheranosticsJanuary 1, 2021Lu G, Tian S, Sun Y, Dong J, Wang N, Zeng J, Nie Y, Wu K, Han Y, Feng B, et al.

(19) Overexpression of PpSnRK1α in tomato enhanced salt tolerance by regulating ABA signaling pathway and reactive oxygen metabolismBMC Plant BiologyDecember 1, 2020Wen-Ru Wang, Jia-Hui Liang, Gui-Fang Wang, Mao-Xiang Sun, Fu-Tian Peng, Yuan-Song Xiaoactivity was determined by the Universal Kinase Activity Kit (R&D Systems, Minneapolis, MN, United States, EA004) by using AMARA polypeptide as the substrate [59]. Determination of antioxidant capacity and the content

(20) Alleviating chronic ER stress by p38-Ire1-Xbp1 pathway and insulin-associated autophagy in C. elegans neuronsPLOS GeneticsSeptember 28, 2020Liying Guan, Zhigao Zhan, Yongzhi Yang, Yue Miao, Xun Huang, Mei Dingphosphorylation of IRE-1 by active PMK-3 was monitored using a Universal Kinase Activity Kit (Catalog Number EA004, R&D Systems). Purified IRE-1 and 200μM ATP were mixed with purified PMK-3 and coupling phosphatase

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Universal Kinase Activity Kit, 2 Plate from R&D Systems, a Bio-Techne Brand

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Specifications/Features

R&D Systems, a Bio-Techne Brand

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Universal Kinase Activity Kit, 2 Plate from R&D Systems, a Bio-Techne Brand

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