ONE-Glo Luciferase Assay System E6120 from Promega

Product Specs
ItemONE-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6120
Sample TypeMammalian cells
MethodONE-Glo Assay System
Quantity100ml
Detection TargetLuciferase

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Genetic code expansion for single noncanonical amino acid incorporation into SARS-CoV-2 Omicron spike on amber-free lentiviral particlesMethods in EnzymologyJune 25, 2026Wang Xu, Narendra Kumar Gonepudi, Yang Han, Maolin LuA1296 Q5® site-directed mutagenesis kit,NEB,Cat # E0554S ONE-Glo™ luciferase assay system,Promega,Cat # E6120 4x Laemmli sample buffer,Bio-Rad,Cat # 1610747 Experimental Models: Cell Lines,Experimental Models:

(2) Bioorthogonal click-chemistry labeling and visualization of dual noncanonical amino acid-incorporated HIV-1 Env on intact virionsMethods in EnzymologyJune 25, 2026Narendra Kumar Gonepudi, Wang Xu, Harry Baffour Awuah, Maolin LuA1296–1KG Q5® Site-Directed Mutagenesis Kit,NEB,Cat # E0554S ONE-Glo™ Luciferase Assay System,Promega,Cat # E6120 6x Laemmli sample buffer,Thermo scientific,Cat # J61337.AD Experimental Models: Cell Lines,Experimental Models

(3) From iPSC to manufactured iNK cells using CombiCult® screening platformFront Cell Dev BiolJune 11, 2026Marina Tarunina, Sachin Luharia, Matthew Houppermans, Giuseppe D'Agostino, Lam Lam, Michelle Gestwa, Aleksandra Habich-Crayton, Marcia Mata, Juline Guenat, Molly Tregidgo, et al.to plot cytotoxicity graphs. For luciferase-based assay, ONE-Glo Luciferase Assay System (Promega, E6120) was used according to manufacturer’s protocol. Luminescence was measured using CLARIOstar (BMG LABTECH).

(4) ω-3PUFAs Inhibit Gallbladder Cancer Through Enhancing STK31 MethylationMol Nutr Food ResJune 11, 2026Shuang-Xing Li, Wei-Yu Zhu, Hai-Ying Peng, Bin Zhang, Zhu-Ying Lin, Song-Lin Yang, Yi-Yi Zhao, Hui Gao, Shan He, Yuan Liu, et al.cells were established after puromycin selection and validated using ONE‐Glo Luciferase Assay System (E6120, Promega, Madison, USA). The nude mice were also pre‐treated with ω‐3PUFAs (350 mg/kg/day) or corn oil

(5) Live-cell Pick-Seq (LiP-Seq): Interrogating ultra-rare mantle cell lymphoma persistent cells after CART19 therapyBlood AdvancesJune 9, 2026Ran Xu, Gongwei Wu, Siobhan Rice, Matthew Booker, Jenalyn Celine Weekes, Hayden Luke Bell, Yifei Gao, Ajit J. Nirmal, Huiyun Liu, Zoe Ciantra, et al.coincubation, the cell lysate was prepared using the ONE-Glo luciferase assay system (Promega, catalog no. E6120). The luciferase activity was measured with a Synergy Neo2 luminescence microplate reader (Biotek). All

(6) Monocytes Derived From Human Pluripotent Stem Cells Engineered for Detection of PyrogensCell ProliferationMay 21, 2026Jingjing Liu, Bingyu Cai, Tingting Gao, Wumei Wei, Liu Wang, Qi Zhou, Wei Li, Jie Hao, Jun WuLuciferase Reporter Assay The luciferase reporter assay was conducted using the Luciferase Reporter Assay System (E6120, Promega) according to the manufacturer's instructions. Briefly, hESCs or monocyte differentiation from

(7) Predictive modeling of immune escape and antigenic grouping of SARS-CoV-2 variantsJournal of VirologyMay 19, 2026Arshan Nasir, Diana Lee, Laura E. Avena, Daniela Montes Berrueta, Tessa Speidel, Kai Wu, Yadunanda Budigi, Andrea Carfi, Guillaume B. E. Stewart-Jones, Darin Edwardscells for 18 hours at 37°C. At 18 hours after infection, an equal volume of One-Glo reagent (Promega, E6120) was added to the culture medium for readout using a BMG PHERastar-FSX plate reader. The percentage

(8) MHY5456, an FXR Agonist, Ameliorates Hepatic Steatosis and Fibrosis in a Mouse Model of MASLDBiomolecules & TherapeuticsMay 1, 2026Mi-Jeong Kim, Hyejin Kang, Jian Yoo, Sugyeong Ha, Jeongwon Kim, Byeong Moo Kim, Da Eun Park, Hae Young Chung, Donghwan Kim, Hyung Ryong Moon, et al.FXR was measured using the One-Glo Luciferase Assay System (Promega, Madison, WI, USA; catalog number E6120). Luminescence was measured as an indicator of FXR transcriptional activity with a luminescence plate

(9) Developing a multimodal therapy for glioblastoma using oncolytic virus delivering CD19 and EGFRvIII antigens and bi-specific CARsNature CommunicationsApril 9, 2026Jia Li, Shyambabu Chaurasiya, Guihua Sun, Qi Cui, Peng Ye, Yue Qin, Tao Zhou, Xiuli Wang, Yuman Fong, Marcela V. Maus, et al.and subjected to a luciferase reporter assay with the ONE-Glo™ Luciferase Assay System (Promega, Cat# E6120). Flow cytometry analysis ESCs and GBM cells were dissociated with Accutase (Gibco, Cat# A1110501)

(10) Quantitative high-throughput screening identifies small-molecule modulators of Wnt/β-catenin signalingFront PharmacolApril 2, 2026Xing Chen, Shu Yang, Srilatha Sakamuru, Chainarong Sukhawanit, Savannah Wood, Masato Ooka, Ruili Huang, Menghang Xiaand 5% CO2. Luciferase activity was measured using ONE-Glo Luciferase Assay reagent (Promega, Cat #E6120) according to the manufacturer’s instructions. Four µL of reagent was added directly to each well, and

(11) Design-driven optimization of low-cost reagent formulations for reproducible and high-yielding cell-free gene expressionNature communicationsMarch 5, 2026Olsen ML, Copeland CE, Sundberg CA, Aw R, Shaver ZM, Rao G, Swartz JR, Karim AS, Jewett MC.with expressed luciferase were diluted tenfold and mixed with ONE-Glo Luciferase Assay System (Promega E6120) in a 15:1 ONE-Glo to diluted reaction ratio. Luminescence was measured on a BioTek Neo2 plate reader

(12) Pharmacological perturbation of splicing elicits SMG1 reduction: Implications for cancer therapyiScienceFebruary 20, 2026Martin Barainka, Angelina Zheleva, Angela Pérez-Cervera, Helena Villanueva, Daniel Meraviglia-Crivelli, Beatriz Moreno, Fernando PastorSYBR™ Green Master Mix,Applied Biosystems,Cat#4385612 ONE-Glo™ Luciferase Assay System,Promega,Cat#E6120 Zombie Green™ Fixable Viability Kit,BioLegend,Cat#423111 ,, Deposited data,Deposited data,Deposited

(13) Development and characterization of immortalized mouse intestinal epithelial cell linesScientific ReportsFebruary 11, 2026Julie Y. Zhou, Scott T. Espenschied, Qiuhe Lu, Paul E. Karell, Kelsey J. Lewis, Kevin P. Newhall, Yi Han, Meera Bambroo, Amalu Job, Thaddeus S. Stappenbeckwithout LPS (500 ng mL−1) for 4 h. At the experimental endpoint, 50 µL of ONE-Glo Reagent (Promega, Cat# E6120) was added to each well, and luminescence was measured using a BioTek Cytation5 plate reader. Signal

(14) Affinity maturation and light-chain-mediated paratope diversification anticipates viral evolutionCell ReportsJanuary 27, 2026John Dingus, Duck-Kyun Yoo, Sachin Kumar, Yajuan Wang, Md Golam Kibria, Shahab Saghaei, Zahra Allahyari, Jessica W. Chen, Natalie M. Caputo, Jason Hwang, et al.L3000008 ExpiFectamine Kit,Thermo Fisher Scientific,Cat# A14525 ONE-Glo luciferase assay system,Promega,Cat# E6120 EZ-Link™ Sulfo-NHS-LC-Biotin,Thermo Fisher Scientific,CAT# 21338 Cell lines,Cell lines,Cell lines HEK293T,ATCC,Cat#

(15) SLC25A11-mediated reprogramming of mitochondrial redox state and lipid peroxidation confers NRF2-dependent ferroptosis resistance in biliary tract cancerCellular & Molecular Biology LettersJanuary 9, 2026Yu-Yu Lin, Han-Hsi Kuo, Zhao-Jing He, Hsin-Yi Chung, Cheorl-Ho Kim, Yi-Ru Pan, Meng-Ju Wu, Ming-Hsien Chan, Chun-Nan Yeh, Nai-Jung Chiang, et al.signals were measured by using a plate reader (Victor3, PerkinElmer) and ONE-Glo Luciferase assay (#E6120, Promega). Relative luciferase activity was calculated by normalizing luciferase activity to cell viability.

(16) Targeting β-catenin degradation with GSK3β inhibitors induces cell death in acute lymphoblastic leukemiaNature CancerJanuary 8, 2026Kadriye Nehir Cosgun, Huda Jumaa, Mark E. Robinson, Zhangliang Cheng, Salim Oulghazi, Kohei Kume, David Fonseca Arce, Nikol Agadzhanian, Klaus M. Kistner, Etienne Leveille, et al.fetal calf serum. Luciferase activity was measured by adding 100 µl of ONE-Glo buffer from Promega (E6120) using a GloMax plate reader (Promega). Cell viability assay In total, 10,000–40,000 cells were seeded

(17) P329G-engager: a universal mix & match antibody-based adaptor platform for cancer immunotherapyMAbsDecember 16, 2025Marlena Surowka, Diana Darowski, Idil Hutter-Karakoc, Christina Claus, Claudia Ferrara-Koller, Anne Freimoser-Grundschober, Thomas Hofer, Johannes Sam, Reto Gianotti, Andrzej Sobieniecki, et al.After the incubation time, 40 µl of a luciferase substrate, ONE-Glo Luciferase Assay reagent (Promega #E6120) was used, allowing for a measurement of relative luminescence units (RLU). Readout was performed using

(18) SARS-CoV-2 Serological Surveillance of Both Vaccinated and Unvaccinated Zoo Animals with the Identification of a Sloth Bear and a Tapir with Previous InfectionVirusesOctober 31, 2025Marie Arvidson, Yashaswi Raj Subedi, Sandipty Kayastha, Angel Mitchell, Kami Alvarado, Xufang Deng, Karen Terio, Matthew Allender, Leyi Wangthe cells were measured for luciferase activity using the ONE-Glo reagent (Promega, Madison, WI, USA, E6120). Briefly, 50 µL of media was removed from all wells, leaving around 50 µL in each well. 50 µL One-Glo

(19) Benzoxazole Derivatives as Potent FXR and PPARα Dual Agonists With Anti-Fibrotic and Metabolic Regulatory EffectsMedComm (2020)October 26, 2025Mi-Jeong Kim, Dong-Gyun Han, Hyeon Seo Park, Sugyeong Ha, Sang Gyun Noh, Jeongwon Kim, Ji-An Yoo, Byeong Moo Kim, Khas-Erdene Battogtokh, Soohwan Oh, et al.PPARα were measured using the One‐Glo Luciferase Assay System (Promega, Madison, WI; catalog number E6120). Luminescence was recorded using a luminescence plate reader (Berthold Technologies GmbH & Co., Bad

(20) Selective agonists of KIR and NKG2A to evade missing self response of natural killer cellsScientific ReportsSeptember 29, 2025Satoshi Hiura, Yuto Kuwasaki, Yosuke Nishikawa, Takako Kimura, Sayaka Yoshida, Makiko Nakayama, Tomohiro Makino, Suguru Uenofor 2.5–8 h, luciferase activity of each well was detected using a Luciferase Assay System (Promega, E6120), in accordance with the manufacturer’s instructions. The percentage of specific lysis was calculated

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ONE-Glo Luciferase Assay System

ONE-Glo Luciferase Assay System from Promega

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ONE-Glo Luciferase Assay System from Promega

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