ONE-Glo Luciferase Assay System E6120 from Promega

Product Specs
ItemONE-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6120
Detection TargetLuciferase
Sample TypeMammalian cells
MethodONE-Glo Assay System
Quantity100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Quantitative high-throughput screening identifies small-molecule modulators of Wnt/β-catenin signalingFront PharmacolApril 2, 2026Xing Chen, Shu Yang, Srilatha Sakamuru, Chainarong Sukhawanit, Savannah Wood, Masato Ooka, Ruili Huang, Menghang Xiaand 5% CO2. Luciferase activity was measured using ONE-Glo Luciferase Assay reagent (Promega, Cat #E6120) according to the manufacturer’s instructions. Four µL of reagent was added directly to each well, and

(2) Design-driven optimization of low-cost reagent formulations for reproducible and high-yielding cell-free gene expressionNature CommunicationsMarch 5, 2026Meagan L. Olsen, Caroline E. Copeland, Chad A. Sundberg, Rochelle Aw, Zachary M. Shaver, Govind Rao, James R. Swartz, Ashty S. Karim, Michael C. Jewettwith expressed luciferase were diluted tenfold and mixed with ONE-Glo Luciferase Assay System (Promega E6120) in a 15:1 ONE-Glo to diluted reaction ratio. Luminescence was measured on a BioTek Neo2 plate reader

(3) Pharmacological perturbation of splicing elicits SMG1 reduction: Implications for cancer therapyiScienceFebruary 20, 2026Martin Barainka, Angelina Zheleva, Angela Pérez-Cervera, Helena Villanueva, Daniel Meraviglia-Crivelli, Beatriz Moreno, Fernando PastorSYBR™ Green Master Mix,Applied Biosystems,Cat#4385612 ONE-Glo™ Luciferase Assay System,Promega,Cat#E6120 Zombie Green™ Fixable Viability Kit,BioLegend,Cat#423111 ,, Deposited data,Deposited data,Deposited

(4) Development and characterization of immortalized mouse intestinal epithelial cell linesScientific ReportsFebruary 11, 2026Julie Y. Zhou, Scott T. Espenschied, Qiuhe Lu, Paul E. Karell, Kelsey J. Lewis, Kevin P. Newhall, Yi Han, Meera Bambroo, Amalu Job, Thaddeus S. Stappenbeckwithout LPS (500 ng mL−1) for 4 h. At the experimental endpoint, 50 µL of ONE-Glo Reagent (Promega, Cat# E6120) was added to each well, and luminescence was measured using a BioTek Cytation5 plate reader. Signal

(5) Affinity maturation and light-chain-mediated paratope diversification anticipates viral evolutionCell ReportsJanuary 27, 2026John Dingus, Duck-Kyun Yoo, Sachin Kumar, Yajuan Wang, Md Golam Kibria, Shahab Saghaei, Zahra Allahyari, Jessica W. Chen, Natalie M. Caputo, Jason Hwang, et al.L3000008 ExpiFectamine Kit,Thermo Fisher Scientific,Cat# A14525 ONE-Glo luciferase assay system,Promega,Cat# E6120 EZ-Link™ Sulfo-NHS-LC-Biotin,Thermo Fisher Scientific,CAT# 21338 Cell lines,Cell lines,Cell lines HEK293T,ATCC,Cat#

(6) SLC25A11-mediated reprogramming of mitochondrial redox state and lipid peroxidation confers NRF2-dependent ferroptosis resistance in biliary tract cancerCellular & Molecular Biology LettersJanuary 9, 2026Yu-Yu Lin, Han-Hsi Kuo, Zhao-Jing He, Hsin-Yi Chung, Cheorl-Ho Kim, Yi-Ru Pan, Meng-Ju Wu, Ming-Hsien Chan, Chun-Nan Yeh, Nai-Jung Chiang, et al.signals were measured by using a plate reader (Victor3, PerkinElmer) and ONE-Glo Luciferase assay (#E6120, Promega). Relative luciferase activity was calculated by normalizing luciferase activity to cell viability.

(7) Targeting β-catenin degradation with GSK3β inhibitors induces cell death in acute lymphoblastic leukemiaNature CancerJanuary 8, 2026Kadriye Nehir Cosgun, Huda Jumaa, Mark E. Robinson, Zhangliang Cheng, Salim Oulghazi, Kohei Kume, David Fonseca Arce, Nikol Agadzhanian, Klaus M. Kistner, Etienne Leveille, et al.fetal calf serum. Luciferase activity was measured by adding 100 µl of ONE-Glo buffer from Promega (E6120) using a GloMax plate reader (Promega). Cell viability assay In total, 10,000–40,000 cells were seeded

(8) P329G-engager: a universal mix & match antibody-based adaptor platform for cancer immunotherapyMAbsDecember 16, 2025Marlena Surowka, Diana Darowski, Idil Hutter-Karakoc, Christina Claus, Claudia Ferrara-Koller, Anne Freimoser-Grundschober, Thomas Hofer, Johannes Sam, Reto Gianotti, Andrzej Sobieniecki, et al.After the incubation time, 40 µl of a luciferase substrate, ONE-Glo Luciferase Assay reagent (Promega #E6120) was used, allowing for a measurement of relative luminescence units (RLU). Readout was performed using

(9) SARS-CoV-2 Serological Surveillance of Both Vaccinated and Unvaccinated Zoo Animals with the Identification of a Sloth Bear and a Tapir with Previous InfectionVirusesOctober 31, 2025Marie Arvidson, Yashaswi Raj Subedi, Sandipty Kayastha, Angel Mitchell, Kami Alvarado, Xufang Deng, Karen Terio, Matthew Allender, Leyi Wangthe cells were measured for luciferase activity using the ONE-Glo reagent (Promega, Madison, WI, USA, E6120). Briefly, 50 µL of media was removed from all wells, leaving around 50 µL in each well. 50 µL One-Glo

(10) Benzoxazole Derivatives as Potent FXR and PPARα Dual Agonists With Anti-Fibrotic and Metabolic Regulatory EffectsMedComm (2020)October 26, 2025Mi-Jeong Kim, Dong-Gyun Han, Hyeon Seo Park, Sugyeong Ha, Sang Gyun Noh, Jeongwon Kim, Ji-An Yoo, Byeong Moo Kim, Khas-Erdene Battogtokh, Soohwan Oh, et al.PPARα were measured using the One‐Glo Luciferase Assay System (Promega, Madison, WI; catalog number E6120). Luminescence was recorded using a luminescence plate reader (Berthold Technologies GmbH & Co., Bad

(11) Selective agonists of KIR and NKG2A to evade missing self response of natural killer cellsScientific ReportsSeptember 29, 2025Satoshi Hiura, Yuto Kuwasaki, Yosuke Nishikawa, Takako Kimura, Sayaka Yoshida, Makiko Nakayama, Tomohiro Makino, Suguru Uenofor 2.5–8 h, luciferase activity of each well was detected using a Luciferase Assay System (Promega, E6120), in accordance with the manufacturer’s instructions. The percentage of specific lysis was calculated

(12) Site-1 protease is a negative regulator of sarcolipin promoter activityCommunications BiologySeptember 24, 2025Isha Sharma, Meredith O. Kelly, Katelyn Hanners, Ella S. Shin, Muhammad G. Mousa, Shelby Ek, Gretchen A. Meyer, Rita T. Brookheartpost-transfection, luciferase assays were performed using One-Glo Luciferase Assay System (Promega, Cat # E6120) as per manufacturer’s instructions. Luminescence followed by GFP fluorescence was quantified with a

(13) Targeting glioblastoma using oncolytic viruses delivered by human pluripotent stem cell-derived neural progenitor cellsMolecular Therapy OncologySeptember 18, 2025Jianfei Chao, Qi Cui, Peng Ye, Shyambabu Chaurasiya, Jonas Cerneckis, Yuman Fong, Yanhong Shimeasured 24 and 48 h after treatment using the ONE-Glo Luciferase Assay System (Promega, catalog no. E6120). GSC transplantation and oncolytic virus treatment All animal-related work was performed under Institutional

(14) Plasma Functionalization Enables Diffusion Control of Reactive Oxygen SpeciesSmallSeptember 1, 2025Paula Navascués, Flaela Kalemi, Flavia Zuber, Philipp Meier, Ludovica M. Epasto, Michał Góra, Barbara Hanselmann, Svetlana Kucher, Enrica Bordignon, Qun Ren, et al.emission 590 nm) whereas skin sensitization (fold‐induction) was evaluated with One‐Glo Reagent (Promega, E6120, integration time = 1 s/well). Subsequently, EC1.5 values (fold‐induction) and IC50 values of dose‐dependent

(15) Mathematical modeling suggests 14-3-3 proteins modulate RAF paradoxical activationPLOS Computational BiologyAugust 1, 2025Gaurav Mendiratta, Kodye Abbott, Yao-Cheng Li, Jingting Yu, Peter Carlip, Melinda Tong, Jianfeng Huang, Maxim N. Shokhirev, Thomas McFall, Geoffrey M. Wahl, et al.hours. RAF inhibitors were added at indicated concentrations and incubated for 2 hours. One-Glo (Promega E6120) was added to one plate and CellTiter-Glo 2.0 (Promega G9242) was added to another plate. Four technical

(16) Using Reporter Gene Assays to Screen and Identify Chemical Compounds that Modulate Estrogen Receptor ActivityCurrent ProtocolsJuly 28, 2025Masato Ooka, Srilatha Sakamuru, J. David Furlow, Menghang Xiacat. no. H7904) Test compounds of interest One-Glo luciferase assay reagent (Promega, cat. no. E6120) CellTiter-fluor (Promega, cat. no. G6082) Biological safety cabinet (Labconco, cat. no. 302610101,

(17) Requirement for Cyclin D1 Underlies Cell-Autonomous HIF2 Dependence in Kidney CancerCancer discoveryJuly 3, 2025Shirole NH, Kesar D, Lee Y, Goodale A, Syamala S, Kukreja S, Li R, Qiu X, Yu W, Goldman S, et al.determined by neomycin resistance, was confirmed using the ONE-Glo Luciferase Assay System (Promega, E6120) as per the manufacturer’s instructions. (i) For the experiments in Supplementary Fig. S10, the 786-O-Fluc–expressing

(18) Enhancing CAR T‐cell therapy manufacturing efficiency through semi‐automated bioprocessingClin Transl ImmunologyJune 2, 2025Jason Isaacson, Prajakta Bhanap, Nicholas Putnam, Jasmine Padilla, Nujhat Fatima, Max Dotson, Danny Hayoun, Moloud Ahmadi, Gertrude Nonterah, Yongchang Jiassay The cytotoxicity assay was performed using the Promega One‐Glo™ Luciferase Assay System (Cat. No. E6120, Promega Corporation, Madison, WI, USA). Following culture, NALM6 cells, unmodified control cells and

(19) A Selective MAP3K1 Inhibitor Facilitates Discovery of NPM1 as a Member of the NetworkMoleculesApril 30, 2025Lidia Boghean, Sarbjit Singh, Kiran K. Mangalaparthi, Smitha Kizhake, Lelisse Umeta, Donn Wishka, Paul Grothaus, Akhilesh Pandey, Amarnath Natarajanallowed to equilibrate and attain room temperature. A total of 110 μL of ONE-Glo luciferase reagent (Cat# E6120; Promega; Madison, WI, USA) was added per well, and the plates were shaken well. Luminescence (1000

(20) Pan-Variant SARS-CoV-2 Vaccines Induce Protective Immunity by Targeting Conserved EpitopesAdvanced science (Weinheim, Baden-Wurttemberg, Germany)April 1, 2025Shah M, Moon SU, Shin JY, Choi JH, Kim D, Woo HG.h. The luciferase activity was measured according to ONE‐Glo Luciferase Assay System (Promega, USA, E6120) manufacturer's instructions, and a Synergy HTX Fluorometer (BioTek, USA) was used without attenuation.

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ONE-Glo Luciferase Assay System

ONE-Glo Luciferase Assay System from Promega

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ONE-Glo Luciferase Assay System from Promega

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