ONE-Glo Luciferase Assay System E6110 from Promega

Product Specs
ItemONE-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6110
Detection TargetLuciferase
Quantity10ml
Sample TypeMammalian cells
MethodONE-Glo Assay System
Molecular Weight~95kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Intranasal Administration of Isoeugenol Improves Memory Deficits in APP/PS1 Old Mice—A Role Beyond Nrf2 Activation?FASEB JAugust 4, 2026Ana Silva, Sónia Silva, Jéssica Macedo, Patrícia Moreira, Diana Baptista, Joana Bicker, Ana Fortuna, Joana Liberal, Beatriz Rodrigues, Rosa Resende, et al.were then washed once with sterile PBS and lysis buffer (ONE‐Glo Luciferase Assay System; Promega #E6110) was added to the cells and incubated for 20 min at RT protected from light. Luciferase activity was

(2) Transcription Factor MAX Regulates Liver Cancer Cell Growth, Migration, Invasion, and Epithelial–Mesenchymal Transition by Promoting SF3A3 ExpressionWorld Journal of OncologyAugust 1, 2026Gui Zhou, Mei Yu Dai, Xiang Chen, Li Ming Liu, Fa Quan LinAfter 48 h, the cells were lysed and luciferase activity was measured using a luciferase assay kit (#E6110, Promega, Madison, WI, USA) according to the manufacturer’s protocol. The luciferase activity was normalized

(3) A radiopharmaceutical enhances CAR T cells against radio-sensitive and radio-resistant neuroblastoma by tumor sensitization and TME remodelingCell Reports MedicineJuly 21, 2026Constanza Rodriguez, Robert S. Edinger, George Diehl, Jeyshka M. Reyes-González, Woonghee Lee, John Buckley, Jangsuk Oh, Divya Nambiar, Kwamena Baidoo, Reona Okada, et al."RecombiMAb human IgG1 isotype control, anti-hen egg lysozyme",BioXcell,CP174 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, OneGlo assay,Promega,E6110 CellTiter-Glo,Promega,G7572 CCK8 Kit,GenBiosystem,A311 Opal 6-Plex Detection Kit,Akoya Bioscience,NEL871001KT ,, Deposited

(4) Caspase-mediated cleavage events hidden by secondary proteolysis during apoptosisProtein SciJuly 20, 2026Fatemeh Fotouhi, Erik Gomez-Cardona, Jainilkumar Patel, Olivier Julien, Howard Fearnheadprotein was used in triplicate for firefly luciferase activity using the One‐Glo Luciferase Assay System (E6110‐Promega) in a PerkinElmer Victor microplate reader. The firefly luciferase activity was measured for

(5) Next-generation chemogenetic inhibition using a brain-permeant non-prescription agentSignal Transduct Target TherJuly 3, 2026Steven O. Devenish, Sahil D. Patel, Laura V. Ussingkær, Luiz F. Almeida Silva, Olivia Goff, Amy Richardson, Jesse I. Mobbs, Hariprasad Venugopal, David M. Thal, Dimitri M. Kullmannalongside the Glo Lysis buffer (Promega, cat. no. E2661) and ONE-GloTM luciferase (Promega, cat. no. E6110). For each plate, 1 ml of ONE-GloTM luciferase was diluted in 7 ml of Glo Lysis buffer, with 70 μL added

(6) Peptide-MHC-targeted engineered virus-like particles enable selective priming and gene editing of tumor-specific T cellsCell ReportsJune 23, 2026Brian H. Shim, Q. Henry Zhao, Jack A. Queenan, Blake E. Smith, Michael E. Birnbaum, David R. LiuKit,QIAGEN,Cat#12963 MuLV Core Antigen ELISA Kit,Cell Biolabs,Cat#VPK-156 ONE-Glo Luciferase Assay System,Promega,Cat#E6110 MiSeq Reagent Kit v2 (300-cycles),Illumina,Cat#MS-102-2002 MiSeq Reagent Micro Kit v2 (300-cycles),Illumina,Cat#MS-103-1002 NEBuilder HiFi DNA Assembly Master Mix

(7) Ultrasound priming gated by solid tumor hallmarks to guide CAR-T therapySci AdvJune 12, 2026Tianze Guo, Ziyue Zhu, Yunjia Qu, Xi Yu, Linshan Zhu, Yuxuan Wang, Kunshu Liu, Jiaxin Cui, Xue Dong, Chi-Woo Yoon, et al.for specified durations, luminescence was measured using the One-Glo Luciferase Assay kit (Promega, E6110), and cytotoxicity was calculated as the percentage reduction in luminescence relative to target-only

(8) Coronavirus protein interaction mapping in bat and human cells reveals network rewiring governing immune evasion and zoonotic potentialCell Host & MicrobeJune 10, 2026Jyoti Batra, Magdalena Rutkowska, Yuan Zhou, Chengjin Ye, Rithika Adavikolanu, Janet M. Young, Durga Anand, Sooraj Verma, Haripriya Parthasarathy, Martin Gordon, et al.FLO-MIN106D RiboPure kit,Invitrogen,Cat# AM1924 ONE-Glo Luciferase Assay System kit,Promega,Cat# E6110 miRNeasy mini kit,QIAGEN,Cat# 217004 P3 nucleofector kit,Lonza,Cat# V4XP-3032 SE Cell Line 4D-Nucleofector® X Kit S

(9) A Transgenic Mouse Model to Track MRC1-High Macrophages Using In Vivo Optical ImagingInt J Mol SciMay 12, 2026Chintan Chawda, Giorgia Zambito, Natasa Gaspar, Christopher Schliehe, Pieter J. M. Leenen, Clemens Löwik, Laura Mezzanotteresuspended in the provided buffer from the ONE-Glo™ Luciferase Assay System (Promega, Madison, WI, USA, #E6110), followed by taking the readings in the IVIS Spectrum imaging system. In the case of KPC tumor-bearing

(10) Low-Strength Type I Interferon Signaling Promotes CAR T Cell Treatment EfficacyCancer Immunol ResMay 11, 2026Erting Tang, Yifei Hu, Guoshuai Cao, Duy-Thuc Nguyen, Nicholas W. Asby, Nada S. Aboelella, Hanna A. Ruiz, Xiaolei Cai, Wenbo Zhang, Yu Zhao, et al.co-cultures were established with E/T ratio of 1:1. After 6 hours, One-Glo Luciferase substrate (Promega, E6110) was added to each well. Residual tumor cells were quantified via luciferase activity. As a positive

(11) CD4 T-cell platform for delivering interferons as an antiviral countermeasure with a focus on SARS-CoV-2Mol MedMarch 28, 2026Xiaohe Liu, Marvin A. Ssemadaali, Harikrishnan Radhakrishnan, Juan Arredondo, Harold S. Javitz, Satya Dandekar, Parijat BhatnagarCellTiter-Glo™ Luminescent Cell Viability Assay Kit (Promega, Cat# PR-G7570) or One-Glo® assay (Promega, Cat #E6110). HEK-Blue IFN-α/β cells (InvivoGen, Cat# hkb-ifnab) or HEK-Blue IFN-λ cells (InvivoGen, Cat# hkb-ifnl)

(12) Rhabdoid tumors as a novel target for PSMA-directed CAR T cell therapyMolecular Therapy OncologyMarch 19, 2026Aroshi Mitra, Tianyi Zhou, Jacky Wu, Tran Nguyen, Chengtai Yu, Amrita Barua, Luping Huang, Xiaoting Jiang, Rajkumar Venkatramani, Moonsoo M. Jin, et al.enzyme-linked immunosorbent assay (ELISA). To assess cytotoxicity, One-Glo luciferase reagent (Promega, E6110) was added to each well, and bioluminescence was measured using the Synergy Neo2 Multi-Mode Reader (BioTek). ELISA Supernatants

(13) Targeting the FGF19/FGFR4 positive feedback loop to disrupt ERK-mediated tumor progression in head and neck cancerJournal of Translational MedicineMarch 13, 2026Yi-Fang Yang, Pei-Lun Yu, Chih-Yu Chou, Yu-Hsuan LinA plate reader (FLUOstar Omega; BMG Labtech GmbH) and a ONE-Glo™ Luciferase Assay System (Promega, #E6110) were used to assess RFP fluorescence and luciferase activity. FGF19 treatment The TW1.5 cells were

(14) CD28 co-stimulatory domain enhances efficacy of CER T cell therapy compared to 4-1BB in an ovarian cancer mouse modelScientific ReportsMarch 11, 2026Nolan J. Beatty, Min Ma, Payal Goala, Shannon McSain, Sae Bom Lee, Justin C. Boucher, Meredith L. Stone, Yun Pyo Kang, Eduardo Cortes-Gomez, Jianmin Wang, et al.incubation with ONE-Glo™ Luciferase Assay System according to manufacturer’s instructions (Promega; E6110). ID8 luciferase cells were then retrovirally transduced with mouse FSHR H29 virus tagged with GFP and

(15) Metabolic stress sensing by epithelial RXRα links westernization of diet with Crohn’s diseaseCell MetabolismMarch 3, 2026Moritz Meyer, Felix Grabherr, Christina Plattner, Michel V. Hadjihannas, Zhigang Rao, Valentin Marteau, Víctor Alonso López-Agudelo, Julian Schwärzler, Lisa Mayr, Almina Jukic, et al.fatty acids and a positive and negative control for 24h. ONE-Glo™ Luciferase Assay System (Promega, E6110) was used according to manufacturer’s recommendations for luminescence quantification. Briefly, 100μl

(16) Rapid receptor internalization potentiates CD7-targeted lipid nanoparticles for efficient mRNA delivery to T cells and in vivo CAR T-cell engineeringbioRxivJanuary 30, 2026Jianhao Zeng, Tyler Ellis Papp, Awurama Akyianu, Alejandra Bahena, Lanfranco Leo, Faris Halilovic, Hamideh Parhizquantified by measuring luciferase activity using the ONE-Glo™ Luciferase Assay System (Promega, Cat# E6110) according to the manufacturer’s instructions. Humanized mouse model All animal studies were conducted

(17) Polymer-lipid hybrid nanoparticle enhances mRNA delivery and T cell-mediated immunitybioRxivJanuary 29, 2026Xiaolei Cai, Min Chen, Guoshuai Cao, Nicholas Asby, Derek Elli, Haley Gula, Vlad Nicolaescu, Duy-Thuc Nguyen, Xiaodan Huang, Tanushree Dangi, et al.plated into 96-well plates to quantify the FLuc expression by ONE-Glo™ FLuc Assay System (Promega #E6110) and microplate reader (BioTek) and the signals were normalized to day-1 signal of LNP-treated group. In vitro

(18) Targeting and dissociating HIF2α from the molecular chaperone Hsp70 triggers apoptosis in kidney cancerCommunications MedicineJanuary 12, 2026Jennifer A. Heritz, Sarah J. Backe, Angela Pacherille, Sara J. Cayaban, Michael F. Basin, Britannia Smith, Rebecca A. Sager, Michael Daneshvar, Dawn E. Post, Mark R. Woodford, et al.additional 8 h. siRNA targeting HIF2α was used as a control. One-Glo Luciferase reagent (Promega, Cat# E6110) was added for 30 min and luminescence was read using Tecan SPARK. Data was normalized to DMSO control. Synthesis

(19) TRBC2-targeting antibody–drug conjugates for the treatment of T cell cancersNature CancerDecember 22, 2025Jiaxin Ge, Joshua Urban, Sarah R. DiNapoli, Bum Seok Lee, Taha Ahmedna, Tushar D. Nichakawade, Brian J. Mog, Steve Lu, Xuyang Li, Nikita Marcou, et al.for luciferase-expressing cells was assessed using the ONE-Glo luciferase assay (Promega, cat. no. E6110). Cell viability for cells not expressing luciferase was assessed using the CellTiter 96 AQueous One Solution Cell Proliferation Assay

(20) Species-specific AAVR dominates species-tropism of adeno-associated virus (AAV) vectorsResearchSquareDecember 5, 2025Mark Kay, Yuqian Jiang, Yiming Liu, Yuqing Jing, Feijie Zhang, Angela Linchanged back to full cell culture media. Luciferase assay ONE-Glo luciferase assay system (Promega #E6110) was used for testing luciferase assay for most experiments. With cultured cells, wells were washed

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ONE-Glo Luciferase Assay System

ONE-Glo Luciferase Assay System from Promega

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ONE-Glo Luciferase Assay System from Promega

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