ONE-Glo Luciferase Assay System E6110 from Promega

Product Specs
ItemONE-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE6110
Detection TargetLuciferase
Quantity10ml
Sample TypeMammalian cells
MethodONE-Glo Assay System
Molecular Weight~95kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) CD4 T-cell platform for delivering interferons as an antiviral countermeasure with a focus on SARS-CoV-2Mol MedMarch 28, 2026Xiaohe Liu, Marvin A. Ssemadaali, Harikrishnan Radhakrishnan, Juan Arredondo, Harold S. Javitz, Satya Dandekar, Parijat BhatnagarCellTiter-Glo™ Luminescent Cell Viability Assay Kit (Promega, Cat# PR-G7570) or One-Glo® assay (Promega, Cat #E6110). HEK-Blue IFN-α/β cells (InvivoGen, Cat# hkb-ifnab) or HEK-Blue IFN-λ cells (InvivoGen, Cat# hkb-ifnl)

(2) Rhabdoid tumors as a novel target for PSMA-directed CAR T cell therapyMolecular Therapy OncologyMarch 19, 2026Aroshi Mitra, Tianyi Zhou, Jacky Wu, Tran Nguyen, Chengtai Yu, Amrita Barua, Luping Huang, Xiaoting Jiang, Rajkumar Venkatramani, Moonsoo M. Jin, et al.enzyme-linked immunosorbent assay (ELISA). To assess cytotoxicity, One-Glo luciferase reagent (Promega, E6110) was added to each well, and bioluminescence was measured using the Synergy Neo2 Multi-Mode Reader (BioTek). ELISA Supernatants

(3) Targeting the FGF19/FGFR4 positive feedback loop to disrupt ERK-mediated tumor progression in head and neck cancerJournal of Translational MedicineMarch 13, 2026Yi-Fang Yang, Pei-Lun Yu, Chih-Yu Chou, Yu-Hsuan LinA plate reader (FLUOstar Omega; BMG Labtech GmbH) and a ONE-Glo™ Luciferase Assay System (Promega, #E6110) were used to assess RFP fluorescence and luciferase activity. FGF19 treatment The TW1.5 cells were

(4) CD28 co-stimulatory domain enhances efficacy of CER T cell therapy compared to 4-1BB in an ovarian cancer mouse modelScientific ReportsMarch 11, 2026Nolan J. Beatty, Min Ma, Payal Goala, Shannon McSain, Sae Bom Lee, Justin C. Boucher, Meredith L. Stone, Yun Pyo Kang, Eduardo Cortes-Gomez, Jianmin Wang, et al.incubation with ONE-Glo™ Luciferase Assay System according to manufacturer’s instructions (Promega; E6110). ID8 luciferase cells were then retrovirally transduced with mouse FSHR H29 virus tagged with GFP and

(5) Metabolic stress sensing by epithelial RXRα links westernization of diet with Crohn’s diseaseCell MetabolismMarch 3, 2026Moritz Meyer, Felix Grabherr, Christina Plattner, Michel V. Hadjihannas, Zhigang Rao, Valentin Marteau, Víctor Alonso López-Agudelo, Julian Schwärzler, Lisa Mayr, Almina Jukic, et al.fatty acids and a positive and negative control for 24h. ONE-Glo™ Luciferase Assay System (Promega, E6110) was used according to manufacturer’s recommendations for luminescence quantification. Briefly, 100μl

(6) Rapid receptor internalization potentiates CD7-targeted lipid nanoparticles for efficient mRNA delivery to T cells and in vivo CAR T-cell engineeringbioRxivJanuary 30, 2026Jianhao Zeng, Tyler Ellis Papp, Awurama Akyianu, Alejandra Bahena, Lanfranco Leo, Faris Halilovic, Hamideh Parhizquantified by measuring luciferase activity using the ONE-Glo™ Luciferase Assay System (Promega, Cat# E6110) according to the manufacturer’s instructions. Humanized mouse model All animal studies were conducted

(7) Polymer-lipid hybrid nanoparticle enhances mRNA delivery and T cell-mediated immunitybioRxivJanuary 29, 2026Xiaolei Cai, Min Chen, Guoshuai Cao, Nicholas Asby, Derek Elli, Haley Gula, Vlad Nicolaescu, Duy-Thuc Nguyen, Xiaodan Huang, Tanushree Dangi, et al.plated into 96-well plates to quantify the FLuc expression by ONE-Glo™ FLuc Assay System (Promega #E6110) and microplate reader (BioTek) and the signals were normalized to day-1 signal of LNP-treated group. In vitro

(8) Targeting and dissociating HIF2α from the molecular chaperone Hsp70 triggers apoptosis in kidney cancerCommunications MedicineJanuary 12, 2026Jennifer A. Heritz, Sarah J. Backe, Angela Pacherille, Sara J. Cayaban, Michael F. Basin, Britannia Smith, Rebecca A. Sager, Michael Daneshvar, Dawn E. Post, Mark R. Woodford, et al.additional 8 h. siRNA targeting HIF2α was used as a control. One-Glo Luciferase reagent (Promega, Cat# E6110) was added for 30 min and luminescence was read using Tecan SPARK. Data was normalized to DMSO control. Synthesis

(9) TRBC2-targeting antibody–drug conjugates for the treatment of T cell cancersNature CancerDecember 22, 2025Jiaxin Ge, Joshua Urban, Sarah R. DiNapoli, Bum Seok Lee, Taha Ahmedna, Tushar D. Nichakawade, Brian J. Mog, Steve Lu, Xuyang Li, Nikita Marcou, et al.for luciferase-expressing cells was assessed using the ONE-Glo luciferase assay (Promega, cat. no. E6110). Cell viability for cells not expressing luciferase was assessed using the CellTiter 96 AQueous One Solution Cell Proliferation Assay

(10) Species-specific AAVR dominates species-tropism of adeno-associated virus (AAV) vectorsResearchSquareDecember 5, 2025Mark Kay, Yuqian Jiang, Yiming Liu, Yuqing Jing, Feijie Zhang, Angela Linchanged back to full cell culture media. Luciferase assay ONE-Glo luciferase assay system (Promega #E6110) was used for testing luciferase assay for most experiments. With cultured cells, wells were washed

(11) Telocytes deliver essential Wnts directly to murine intestinal stem cells via synapse-like contactsDevelopmental CellNovember 17, 2025Gediminas Greicius, Lorenz Mittermeier, Ruanyi Liang, Kristmundur Sigmundsson, Yarn Kit Chan, Pei-Ju Liao, Alexander Ludwig, David M. Virshupassays,Critical commercial assays,Critical commercial assays ONE-Glo™ Luciferase Assay System,Promega,Cat#E6110 ,, Experimental models: Cell lines,, Human: STF cells,Coombs et al.85,N/A Human: HEK293FT cells,Thermo Fisher SCIENTIFIC

(12) A peptide display system identifies a potent mutant β-melanocyte-stimulating hormone agonist of melanocortin-4 receptorCell GenomicsNovember 12, 2025Ann Lin, Kaitlyn Spees, Raeline Valbuena, Jakob Wirbel, Aravind Natarajan, Nora Enright, Ami S. Bhatt, Michael C. Bassikcoupled receptor. MC4R activation was measured using the ONE-Glo Luciferase Assay (Promega, cat no. E6110). Peptide display assay To measure MC4R activation by the cis-displayed peptides, 100,000 MC4R HTLA

(13) CEA-4-1BBL: CEACAM5-Targeted 4-1BB Ligand Fusion Proteins for Cis Co-Stimulation with CEA-TCBAntibodies (Basel)November 7, 2025Christina Claus, Claudia Ferrara-Koller, Johannes Sam, Sabine Lang, Rosmarie Albrecht, Regula B. Buser, Esther Bommer, Grégory La Sala, Valeria G. Nicolini, Sara Colombetti, et al.titrated in 1:5 dilution steps). Afterwards, One-Glo Luciferase reagent (Promega, Walldorf, Germany E6110) was added, and luminescence was measured for 0.5 s/well using a Tecan Spark plate reader. Human PBMC

(14) Modeling and addressing on-target/off-tumor toxicity of claudin 18.2 targeted immunotherapiesNature CommunicationsNovember 1, 2025Elizabeth J. Carstens, Kazuki Takahashi, Naoya Sakamoto, Martina De Vizio, Micaela Morgado, Shahryar Khoshtinat Nikkhoi, Abhishek Mangipudi, Canh Hiep Nguyen, Tate Weltzin, Izuma Nakayama, et al.incubated for 72 h. Cell viability was determined by a luciferase-dependent assay with OneGlo substrate E6110 (Promega Madison, WI, USA), where % cytotoxicity = (BLI Control− BLISAMPLE)/BLIMAX; BLI Control = mean

(15) Lifespan‐Regulated CAR‐Macrophages from Myeloid Progenitors for Enhanced Colorectal Cancer TherapyAdvanced ScienceNovember 1, 2025Chuancheng Gao, Fangling Hong, Yao Dong, Yong Fu, Yunong Ma, Xuedi Sun, Junfeng Zhang, Jiangning Chen, Zhen Huangbioluminescence was detected according to the instructions of the ONE‐Glo™ Luciferase Assay System (cat. no.: E6110, Promega, Wisconsin, USA). The tumor cell lysis ratio (%) was calculated using the following formula:

(16) AI-Validated Brain Targeted mRNA Lipid Nanoparticles with Neuronal TropismACS NanoOctober 21, 2025Mor Sela, Gal Chen, Haim Kadosh, Tomer Kagan, Raneen Nicola, Sally Turutov, Yuval Richtman, Lin Zhige, Mia R. Albalak Menasherov, Shaked Kagan, et al.BioTechnologies, US) LNPs for 16 h. To measure luciferase expression, 20 μL of ONE-Glo Luciferase Assay System (E6110; Promega, US) was added to the media. Finally, the luminescent signal of the plates was measured using

(17) Targeting mutated KRAS by HLA-A*02:01 restricted anti-KRAS TCR-mimic CAR and bispecific T cell engagerJournal of Molecular MedicineOctober 1, 2025Saber Ebrahimi, Benedikt J. Lohnes, Shamsul A. Khan, Matthias Peipp, Ernesto Bockamp, Christian Klein, Hinrich Abken, Catherine Wölfel, Matthias Theobald, Udo F. Hartwigwas then aspirated from the cells. The cells were resuspended in 100 mL of ONE-Glo Luciferase Assay (E6110, Promega). After 30 min of incubation, the relative luminescence units (RLU) were determined using a

(18) Encephalomyocarditis virus protein 2B* interacts with 14-3-3 proteins through a phosphorylated C-terminal binding motifmBioSeptember 10, 2025Samantha K. Nguyen, Nadine Anders, Stephen Holmes, Henry G. Barrow, Nina Lukhovitskaya, Aminu S. Jahun, Iliana Georgana, Laura G. Caller, James R. Edgar, Stephen C. Graham, et al.thawed simultaneously, and firefly luciferase activity was measured (One-Glo Luciferase System, Promega E6110). Quantitative RT-PCR Following transfection of MEF cells, the media were changed prior to the addition

(19) Hemizygous IL2RG Variants Impair IL-2-Induced STAT5 Phosphorylation and Transcriptional Activity Causing X-Linked Severe Combined ImmunodeficiencyThe Application of Clinical GeneticsSeptember 6, 2025Ning Zhang, Yi-Lin Sang, Wu Zhu, Yu-Rong Wang, Yan-Yan Yu, Ya-Hui Chen, Juan Du, Wen-Bin He, Yue-Qiu Tan, Fu-Yan Wangthen lysed, and luciferase activity was measured using the ONE-Glo™ Luciferase Assay System (Promega, E6110, Madison, WI, USA), according to the manufacturer’s recommendations. Immunoprecipitation-Mass Spectrometry HEK293 cells

(20) NAD+ Metabolism-Mediated SURF4-STING Axis Enhances T-Cell Anti-Tumor Effects in the Ovarian Cancer MicroenvironmentCell Death DisAugust 23, 2025Jiacheng Shen, Fangfang Xu, Tingwei Liu, Yingjun Ye, Shaohua Xuculture plates for co-culture for 24 h. The next day, 100 μL of ONE-Glo Luciferase Assay System reagent (E6110, Promega) was added to each well. After standing for 3 min to ensure complete cell lysis, the luminescence

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ONE-Glo Luciferase Assay System

ONE-Glo Luciferase Assay System from Promega

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ONE-Glo Luciferase Assay System from Promega

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