ELISA Wash Buffer (20X) 421601 from BioLegend

Product Specs
ItemELISA Wash Buffer (20X)
CompanyBioLegend
Price $141.00Supplier Page View Company Product Page
Catalog Number421601
ApplicationsELISA
Quantity500 mL
Format20X concentrated Phosphate buffered saline (pH7.2) with Tween-20

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Highly Effective mRNA-LNP Vaccine Against Respiratory Syncytial Virus (RSV) in Multiple ModelsVaccines (Basel)June 10, 2025Huarong Bai, Xueliang Yu, Yue Gao, Qin Li, Baigang Wen, Rongkuan Huthe specific antigen from the refrigerator at 4 °C and washed once with washing solution (BIOLEGEND, 421601, San Diego, CA, USA). Then, 100 µL of blocking solution was added to each well, and the plate was sealed

(2) A monoclonal anti-hemagglutinin stem antibody modified with zanamivir protects against both influenza A and B virusesProceedings of the National Academy of Sciences of the United States of AmericaApril 15, 2025Liu X, Balligand T, Le Gall C, Ploegh HL.NA at 4 °C overnight. The plates were then washed three times with ELISA wash buffer (catalog number 421601, BioLegend), blocked with blocking buffer (1% bovine serum albumin in PBS) for 1 h at room temperature

(3) Inhibition of ULK1/2 and KRASG12C controls tumor growth in preclinical models of lung cancereLifeAugust 30, 2024Phaedra C Ghazi, Kayla T O'Toole, Sanjana Srinivas Boggaram, Michael T Scherzer, Mark R Silvis, Yun Zhang, Madhumita Bogdan, Bryan D Smith, Guillermina Lozano, Daniel L Flynn, et al.a 4°C refrigerated centrifuge. ELISA plates were washed twice with 1× ELISA wash buffer (BioLegend, 421601), blocked for 1 hr at room temperature (RT) with 1× ELISA dilutant buffer (BioLegend Cat# 421203), then

(4) A novel method for identifying SARS-CoV-2 infection mutants via an epitope-specific CD8+ T cell testBiosafety and HealthJune 1, 2024Congling Qiu, Bo Peng, Chanchan Xiao, Pengfei Chen, Lipeng Mao, Xiaolu Shi, Zhen Zhang, Ziquan Lv, Qiuying Lv, Xiaomin Zhang, et al.(BioLegend, Cat#270302, US) at RT (18–25 °C) for 16-18 h, washed 3 times with washing buffer (BioLegend Cat#421601, US) and blocked with dilution buffer (0.5 mol/L Tris pH 8.0, 1 mol/L NaCl, 1 % BSA, 0.2 % Tween 20)

(5) Identification and affinity enhancement of T-cell receptor targeting a KRASG12V cancer neoantigenCommunications BiologyApril 29, 2024Mengyu Zhang, Wei Xu, Lingjie Luo, Fenghui Guan, Xiangyao Wang, Pei Zhu, Jianhua Zhang, Xuyu Zhou, Feng Wang, Sheng Yeincubated at 37 °C for 1 hour. Post-incubation, the plates were washed with 1× wash buffer (Biolegend, Cat. 421601). Subsequently, 100 microliters of diluted HRP-conjugated anti-human β2-microglobulin antibody (Biolegend,

(6) Insufficient epitope-specific T cell clones are responsible for impaired cellular immunity to inactivated SARS-CoV-2 vaccine in older adultsNat AgingApril 1, 2023Xiao C, Ren Z, Zhang B, Mao L, Zhu G, Gao L, Su J, Ye J, Long Z, Zhu Y, et al.at room temperature (18–25 °C) for 16–18 hours, washed three times with washing buffer (BioLegend, 421601) and blocked with dilution buffer (0.5 M Tris pH 8.0, 1 M NaCl, 1% BSA and 0.2% Tween 20) at room temperature

(7) Production and purification of infectious diseases-associated tetramer for staining of CD8(+) T cells from PBMCsSTAR protocolsMarch 18, 2022Ye J, Lei W, Xiao C, Su X, Gao J, Li X, Li W, Wang P, Jiang Z, Chen G.(DTT),Beyotime,Cat#ST-043 EDTA ph8.0 (0.5 M),KeyGen BioTECH,Cat#KGR0084 ELISA Wash Buffer 20× concentrate,BioLegend,Cat#421601 ELISA Coating Buffer (5×),BioLegend,Cat#421701 Glutathione oxidized,Sangon biotech,Cat#A610228-0001 Glutathione

(8) A Spike Protein-Based Subunit SARS-CoV-2 Vaccine for Pets: Safety, Immunogenicity, and Protective Efficacy in Juvenile CatsFrontiers in Veterinary ScienceMarch 14, 2022Kairat Tabynov, Madiana Orynbassar, Leila Yelchibayeva, Nurkeldi Turebekov, Toktassyn Yerubayev, Nurali Matikhan, Tlektes Yespolov, Nikolai Petrovsky, Kaissar Tabynovshaker, Biosan) for 1 h at room temperature. The plates were washed four times with ELISA Wash Buffer (# 421601, BioLegend). Juvenile cat serum samples were titrated twofold from dilutions 1:160–1:163,840, and 100

(9) An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmissionNPJ vaccinesFebruary 23, 2022Tabynov K, Turebekov N, Babayeva M, Fomin G, Yerubayev T, Yespolov T, Li L, Renukaradhya GJ, Petrovsky N, Tabynov K.shaker, BIOSAN) for 1 h at room temperature. The plates were washed four times with ELISA Wash Buffer (# 421601, BioLegend). Mouse serum samples were titrated two-fold from dilutions 1:125 to 1:32,000 (after prime

(10) Evaluating TNF-α and Interleukin-2 (IL-2) Levels in African American Primary Open-Angle Glaucoma PatientsGenesDecember 25, 2021Alapati T, Sagal KM, Gudiseva HV, Pistilli M, Pyfer M, Chavali VRM, O'Brien JM.on each plate. The plate was washed four times with at least 300 µL of Wash Buffer (Biolegend Cat. #421601, Biolegend, San Diego, CA, USA). Residual buffer was blotted by firmly tapping the plate upside down

(11) CD8⁺ T-Cell Epitope Variations Suggest a Potential Antigen HLA-A2 Binding Deficiency for Spike Protein of SARS-CoV-2Frontiers in immunologyJanuary 1, 2021Qiu C, Xiao C, Wang Z, Zhu G, Mao L, Chen X, Gao L, Deng J, Su J, Su H, et al.California, USA) at room temperature (18–25°C) for 16–18 h, washed 3 times with Washing Buffer (BioLegend Cat#421601, San Diego, California, USA), and blocked with Dilution Buffer (0.5 M Tris, pH 8.0, 1 M NaCl, 1% BSA,

(12) Destabilizing single chain major histocompatibility complex class I protein for repurposed enterokinase proteolysisScientific reportsSeptember 10, 2020Lim J.overnight. The next day, unbound anti-human β2m was washed out with 1xELISA wash buffer (BioLegend, cat no. 421601) diluted in water and blocked with 1 × ELISA diluent B (BioLegend, cat no. 421205) diluted in phosphate

(13) Nasal vaccination with poly(β-amino ester)-poly(d,l-lactide-co-glycolide) hybrid nanoparticlesInternational journal of pharmaceuticsAugust 30, 2017Sinani G, Sessevmez M, Koray Gök M, Özgümüş S, Okyar A, Oya Alpar H, Cevher E.

(14) DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer modelGene TherapyOctober 1, 2014T-Y Weng, M-C Yen, C-T Huang, J-J Hung, Y-L Chen, W-C Chen, C-Y Wang, J-Y Chang, M-D LaiDiego, CA, USA) were used with modiﬁcation. The coating buffer (cat. no. 79008), wash buffer (cat. no. 421601), assay diluent (cat. no. 78888) and substrate solution F (cat. no. 79057) were purchased from BioLegend

(15) Ultrasensitive isolation, identification and quantification of DNA-protein adducts by ELISA-based RADAR assayNucleic acids researchJuly 1, 2014Kiianitsa K, Maizels N.

(16) Effect of dibenzepin hydrochloride on the oxygen, glucose uptake and oxidative phosphorylation of rat brain in vitroIl Farmaco; edizione scientificaSeptember 1, 1977A Velasco-Martín, R Pérez Bejarano, J L González de Zárate, I Saiz-Martínez-Acitores, A Moreno González

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ELISA Wash Buffer (20X) from BioLegend

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ELISA Wash Buffer (20X) from BioLegend

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