ELISA Coating Buffer (5X) from BioLegend

Product Specs
ItemELISA Coating Buffer (5X)
CompanyBioLegend
Price $19.00Supplier Page View Company Product Page
Catalog Number421701
Quantity30 mL
ApplicationsELISA - Quality tested
Format5X concentrated carbonate buffer, pH 9.5

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Intravitreal AAV vector delivery induces integrin-dependent ocular inflammation, complement activation, and antiviral and DNA damage responsesFrontiers in ImmunologyApril 22, 2026Helena Costa-Verdera, Deepa Jamwal, Emily Fabyanic, Ariel Hippen, Madeleine Salvatore, Aiping Du, Anna Majowicz, David Markusic, Jon E. Chatterton, Virginia Haurigot, et al.Radnor, PA) at 1x109 vg/well in 50µl per well (2x1010 vg/ml) diluted in ELISA coating buffer (Biolegend, #421701). For standard curve, purified IgG (Southern Biotech, Swine IgG-UNLB # 0137-01) was serially diluted

(2) Inhibition of MAPK p38α overcomes the cancer immunosurveillance defect caused by FPR1 loss-of-function mutationCell Rep MedMarch 17, 2026Yuhong Pan, Liwei Zhao, Jiani Liu, Misha Mao, Ai-Ling Tian, Julie Le Naour, Sarah Adriana Scuderi, Hui Pan, Flora Doffe, Donal Naylor, et al.antibodies were diluted 1:500 in 1× ELISA coating buffer (prepared from 5× ELISA coating buffer, Cat# 421701, Biolegend) and applied at 100 μL per well in 96-well high-binding assay plates (Cat# 9018, Corning).

(3) Engineered antibodies that stabilize drug-modified KRASG12C neoantigens enable selective and potent cross-HLA immunotherapyNat CommunDecember 17, 2025Lorenzo Maso, Sarah A. Mosure, Sergio A. Rodriguez-Aponte, Angelina Pizzo, Diamond N. Mensah, Matthew Southard, Samantha Sze, Tanvir Ahmed, Brian Vash, Takamitsu Hattori, et al.HLA Class I ELISA assay (BioLegend) according to the manufacturer’s instructions (BioLegend, 280301, 421701, 280302, 280303, 423501). Antigen characterization p/p*MHCs stability analysis Stability of p*MHCs

(4) Single-Cell Analysis Reveals a Critical Role for Macrophage Epsins in Regulating the Origin of Foam Cells in AtherosclerosisArteriosclerosis, thrombosis, and vascular biologyNovember 1, 2025Arulsamy K, Cui K, Zhu B, Wang B, Eisa-Beygi S, Voronova A, Gao X, Gupta K, Zhang L, Chen K, et al.100 μL/well of capture antibody at a final concentration of 0.5 μg/mL diluted in coating buffer (Cat# 421701, BioLegend). The following day, plates were washed three times with PBS containing 0.05% Tween-20 (PBST)

(5) Neocortical tau propagation is a mediator of clinical heterogeneity in Alzheimer’s diseaseMolecular PsychiatrySeptember 1, 2025Anjalika Chongtham, Aarthi Ramakrishnan, Marissa Farinas, Diede W. M. Broekaart, Joon Ho Seo, Carolyn W. Zhu, Mary Sano, Li Shen, Ana C. Pereira423501) were coated with Tau-5 (Invitrogen, #AHB0042; 0.5 μg/mL) in ELISA Coating Buffer (BioLegend; 421701) overnight at 4 °C. Plates were washed twice with PBST (0.05% Tween 20), blocked with 1% BSA/PBS for

(6) Development of an indirect enzyme-linked immunosorbent assay based on nucleocapsid protein for the detection of swine acute diarrhea syndrome coronavirus antibodyVirol JJune 20, 2025Yu-Jeong Jang, Nam Phuong Le, Eun-Song Lee, Min-Chae Kim, Tae-Kyung Chang, Jung-Eun ParkSciences) were incubated with 100 µl recombinant N protein in ELISA coating buffer (BioLegend, Cat. No. 421701) overnight at 4 °C. The plates were washed three times with phosphate-buffered saline containing 0.05%

(7) Low-dose irradiation of the gut improves the efficacy of PD-L1 blockade in metastatic cancer patientsCancer CellMarch 10, 2025Jianzhou Chen, Antonin Levy, Ai-Ling Tian, Xuehan Huang, Guoxin Cai, Marine Fidelle, Conrad Rauber, Pierre Ly, Eugénie Pizzato, Lisa Sitterle, et al.Doxycycline Hyclate,Sigma-Aldrich,D3000000 Recombinant murine IL-2_ELISA standard,BioLegend,575409 ELISA Coating Buffer (5x),BioLegend,421701 HRP-Avidin,BioLegend,405103 1-Step Ultra TMB-ELISA Substrate Solution,Life Technologies,34028 Bile

(8) The guided fire from within: intratumoral administration of mRNA-based vaccines to mobilize memory immunity and direct immune responses against pathogen to target solid tumorsCell discoveryJanuary 2, 2025Li R, Hu JC, Rong L, He Y, Wang X, Lin X, Li W, Wu Y, Kuwentrai C, Su C, et al.Pierce Mem-PER Plus Membrane Protein Extraction Kit, Thermo Fisher, 89842) in coating buffer (BioLegend, 421701) and incubated at 4 °C overnight. The plates were blocked by blocking buffer (BIO-RAD, 1706404, the

(9) Protocol for developing Pseudomonas aeruginosa type III secretion system-neutralizing monoclonal antibodies from human B cellsSTAR ProtocolsDecember 20, 2024Alexandra Albus, Christoph Kreer, Florian Klein, Jan Rybniker, Alexander Simonis14190250 DTT,Promega,Cat.#P1171 EcoRI-HF,NEB,Cat.#R3101 EDTA,Thermo Fisher Scientific,Cat.# AM9260G ELISA coating buffer,BioLegend,Cat.# 421701 Ethanol,Carl Roth,Cat.# 9065.4 Fetal calf serum (FCS),Thermo Fisher Scientific,Cat.# 10270-106 Ficoll-Paque Plus

(10) High-throughput investigation of genetic design constraints in domesticated Influenza A Virus for transient gene deliverybioRxivFebruary 20, 2024Adam Sychla, Christopher S Stach, Shanley N Roach, Amanda N Hayward, Ryan A Langlois, Michael J Smanskiμg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1 × ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML) in PBS, blocked with 200 μL of 3% BSA in

(11) Quantification of Autoreactive Antibodies in Mice upon Experimental Autoimmune EncephalomyelitisJournal of Visualized ExperimentsDecember 18, 2023Jonathan J. Carver, Alessandro Didonnatray,Fisher,S111022, Dissector scissors,Fine Science Tools,14082-09, ELISA coating buffer,BioLegend,421701,Store at 4°C Excel software,Microsoft,,Analysis spreadsheet Forceps,Fine Science Tools,11152-10, "Goat Anti-Mouse IgG, Human ads-HRP

(12) PD-1 Impairs CD8+ T Cell Granzyme B Production in Aged Mice during Acute Viral Respiratory InfectionImmunoHorizonsNovember 1, 2023Parks OB, Antos D, Eddens T, Walters S, Johnson M, Oury TD, Gottschalk RA, Erickson JJ, Williams JV.of TN/94-49 HMPV viral stock per well was diluted in 1× ELISA coating buffer (BioLegend, catalog no. 421701) and plated overnight at 4°C. The remainder of the ELISA was performed as in (32). Flow cytometry staining Flow cytometry

(13) An Animal Model for Chronic Meningeal Inflammation and Inflammatory Demyelination of the Cerebral CortexInternational journal of molecular sciencesSeptember 9, 2023Enz LS, Winkler A, Wrzos C, Dasen B, Nessler S, Stadelmann C, Schaeren-Wiemers N.IFNγ capture antibody (diluted 1:500, 507802, BioLegend), respectively, was diluted in coating buffer (421701, BioLegend) and 100 μL per well was distributed in a 96-well plate (423501, Biolegend). After 18 h of

(14) Tumor and local lymphoid tissue interaction determines prognosis in high-grade serous ovarian cancerCell Reports MedicineJuly 18, 2023Haonan Lu, Hantao Lou, Georg Wengert, Reema Paudel, Naina Patel, Saral Desai, Bill Crum, Kristofer Linton-Reid, Mitchell Chen, Dongyang Li, et al.MAB11012), or 0.8ug/mL anti-human GZMB antibody (R&D, DY2906-05) diluted in coating buffer (Biolegend, 421701). The coated plate was then blocked with 1X Assay Diluent A (Biolegend) and washed with 0.05% PBST.

(15) Human Metapneumovirus Reinfection in Aged Mice Recapitulates Increased Disease Severity in Elderly Humans Infected with Human MetapneumovirusImmunoHorizonsJune 1, 2023Parks OB, Eddens T, Zhang Y, Oury TD, McElroy A, Williams JV.served as control. IgG HMPV ELISA A total of 1 μg of TN/94-49 HMPV in 1× ELISA coating buffer (Cat# 421701; BioLegend) was plated per well overnight at 4°C. The remainder of the ELISA was performed as in Alvarez

(16) Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8(+) T cellsNature immunologyMay 1, 2023Corria-Osorio J, Carmona SJ, Stefanidis E, Andreatta M, Ortiz-Miranda Y, Muller T, Rota IA, Crespo I, Seijo B, Castro W, et al.homemade-ELISA: coating antibody: anti-mouse PD-1 (R&D, AF1021, final concentration of 2 µg ml−1; BioLegend, 421701). Detection antibody: anti-hIgG4-HRP (clone HP6023, Abcam; 1:2,000 dilution). IL-2v homemade ELISA:

(17) Alleviation of arthritis through prevention of neutrophil extracellular traps by an orally available inhibitor of protein arginine deiminase 4Scientific reportsFebruary 23, 2023Gajendran C, Fukui S, Sadhu NM, Zainuddin M, Rajagopal S, Gosu R, Gutch S, Fukui S, Sheehy CE, Chu L, et al.chicken type II collagen (catalog number: 20012, Chondrex) in ELISA coating buffer (catalog number: 421701, BioLegend, Inc., San Diego, CA) overnight at 4 °C. After washing with PBS plus 0.05% Tween-20, the

(18) Primary and secondary functions of HLA-E are determined by stability and conformation of the peptide-bound complexesCell ReportsJune 14, 2022Lucy C. Walters, Daniel Rozbesky, Karl Harlos, Max Quastel, Hong Sun, Sebastian Springer, Robert P. Rambo, Fiyaz Mohammed, E. Yvonne Jones, Andrew J. McMichael, et al.Medical Center, Leiden, The Netherlands",https://ccb.lumc.nl/ ELISA Coating buffer(x5),Biolegend,Cat#421701 ELISA Wash Buffer (x20),Biolegend,Cat#421501 TMB High sensitivity Substrate,Biolegend,Cat#421501 Stop

(19) Antibody profiling of environmental protein toxins and virulence factors reveals the prevalence, persistence, and genetics of antibody responsesImmunityJune 1, 2022Julia W. Angkeow, Daniel R. Monaco, Athena Chen, Thiagarajan Venkataraman, Sahana Jayaraman, Cristian Valencia, Brandon M. Sie, Thomas Liechti, Payam Noroozi Farhadi, Gabriela Funez-dePagnier, et al.S7653 Tween-20,Sigma-Aldrich,Cat. # P1379 NP-40,Sigma-Aldrich,Cat. # 492016 ELISA Coating Buffer (5X),BioLegend,Cat. # 421701 Pierce Streptavidin Magnetic Beads,ThermoFisher,Cat. # 88817 QuickPlex 96-Well Plate Plate Pack,Meso

(20) Production and purification of infectious diseases-associated tetramer for staining of CD8(+) T cells from PBMCsSTAR protocolsMarch 18, 2022Ye J, Lei W, Xiao C, Su X, Gao J, Li X, Li W, Wang P, Jiang Z, Chen G.BioTECH,Cat#KGR0084 ELISA Wash Buffer 20× concentrate,BioLegend,Cat#421601 ELISA Coating Buffer (5×),BioLegend,Cat#421701 Glutathione oxidized,Sangon biotech,Cat#A610228-0001 Glutathione reduced,Sangon biotech,Cat#A600229-0005 High

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ELISA Coating Buffer (5X) from BioLegend

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ELISA Coating Buffer (5X) from BioLegend

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