SBA Clonotyping System-C57BL/6-HRP 5300-05B from SouthernBiotech

Product Specs
ItemSBA Clonotyping System-C57BL/6-HRP
CompanySouthernBiotech
Price $486.00Supplier Page View Company Product Page
Catalog Number5300-05B
Quantity1 kit
ApplicationsELISA
Conjugate/Tag/LabelHRP (Horseradish Peroxidase)
FormatLiquid - UNLB antibody - Borate buffered saline, pH 8.2 HRP conjugates - 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Research AreaImmunology, Secondary Antibodies
HostGoat

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SouthernBiotech

160 Oxmoor Boulevard

Birmingham, Alabama 35209

United States

Phone: (205) 945-1774
Toll-Free U.S. and Canada: (800) 722-2255
Fax: (205) 945-8768

Company Profile

Website: http://www.southernbiotech.com

(1) Ptpn2 limits plasma cell fate and antiviral immunity by integrating B cell receptor and IFN-γ signals in B cellsSci AdvAugust 21, 2026Ana Maria Hincapie, Alexandre Poirier, Isabelle Aubry, Philippe Aumont, Aanya Bhagrath, Noriko Uetani, Bianca Colalillo, Chenyue Wu, Benoit Charbonneau, Stephanie Bussieres-Marmen, et al.experiment). ELISA ELISAs to detect serum antibody isotypes were done using the SBA Clonotyping System-C57BL/6 (cat. no. 5300-05B, Southern Biotech) and the C57BL/6 Mouse Immunoglobulin Panel (cat. no. 5300-01B, Southern Biotech)

(2) Antibody-mediated diversification of primary and secondary humoral immune responsesJ Exp MedJune 1, 2026Dennis Schaefer-Babajew, Laurine Binet, Gabriela S. Silva Santos, Chiara Ruprecht, Lachlan P. Deimel, Mohamed A. ElTanbouly, Dounia Gharrassi, Gabriella Lima Dos Reis, Clara Uhe, Kai-Hui Yao, et al.Jackson ImmunoResearch or Cat.# 1030-05; Southern Biotech), or mouse IgM, IgG3, IgG1, IgG2b, or IgA (Cat.# 5300-05B; Southern Biotech), or IgG2c (115-035-208; Jackson ImmunoResearch), or total Ig (anti-kappa combined

(3) RNA binding protein Pcbp1 maintains mitochondria integrity to promote antibody production and germinal center responseSci AdvApril 10, 2026Lizhen Zhu, Li Lin, Tuan Qi, Geng Li, Zhixing Liang, Jiancheng Yu, Yifan Fu, Yue Peng, Xing Changwashed again with PBST five times and HRP-conjugated anti-mouse IgG1 (0.5 μg/ml; Southern Biotech, 5300-05B), IgG3 (Southern Biotech, 5300-05B) and was added into each well for 1-hour incubation. After sufficiently

(4) The RNA binding protein Arid5a is an activator of TNF signaling in rheumatoid arthritisJCI InsightJanuary 23, 2026Yang Li, Ipsita Dey, Shachi P. Vyas, Alzbeta Synackova, Decheng Li, Erik Lubberts, Dana P. Ascherman, Peter Draber, Sarah L. Gaffenthe upper back. Anti-chicken or anti-mouse IgG1 and IgG2c were measured by ELISA (SouthernBiotech, 5300-05B) on days –3, 18, and 53. Cell culture, cytokines, and inhibitors. HK-2 cells (ATCC) were cultured

(5) B Lymphocyte Protein Factories produced by Hematopoietic Stem Cell Gene EditingbioRxivJanuary 22, 2026Harald Hartweger, Chiara Ruprecht, Kai-Hui Yao, Philippe Laffont, Gabriella Lima Dos Reis, Pengcheng Zhou, Thomas Hägglöf, Laurine Binet, Maximilian Loewe, Jun P. Hong, et al.Cat.# 115–035-071 or Southern Biotech Cat.# 1030–05) or mouse IgM or mouse IgA (Southern Biotech Cat.# 5300–05B). Binding was revealed using 3,3’,5,5’-tetramethylbenzidine substrate (ThermoFisher, Cat.# 34021) and

(6) T-cell-derived IFN-γ suppresses T follicular helper cell differentiation and antibody responsesThe EMBO journalMay 1, 2025Sala E, Nelli M, Laura C, Di Lucia P, Beccaria CG, Bono EB, Mangione M, Marotta D, Sperto V, Grillo M, et al.the secondary anti-mouse IgM, IgG1 or IgG3 conjugated with horseradish peroxidase (SouthernBiotech # 5300-05B) was added and the plates were incubated for 1 h at room temperature. After washing, the binding of

(7) Multi‐Target Peptide Nanofiber Immunotherapy Diminishes Complement Anaphylatoxin Activity in Acute InflammationAdvanced Healthcare MaterialsJanuary 1, 2025Helena Freire Freire Haddad, Emily F. Roe, Vinicius Xie Xie Fu, Elizabeth J. Curvino, Joel H. Collier1:5000 dilution was used to detect total IgG. SBA Clonotyping System-C57BL/6-HRP (Southern Biotech, Cat #5300–05B) was used to detect IgA (1:1000 dilution) and IgG subclasses (1:5000 dilution). Plates were developed

(8) Tuning Helical Peptide Nanofibers as a Sublingual Vaccine Platform for a Variety of Peptide EpitopesAdvanced Healthcare MaterialsJanuary 1, 2025Emily F Roe, Helena Freire Freire Haddad, Kat M Lazar, Peiying Liu, Joel H Collier1:5000 and added to the plate for 45 min. IgA HRP-conjugated detection antibody (Southern Biotech, 5300–05B) was diluted 1:1000. TMB (ThermoFisher, 00–4201-56) and 1 m phosphoric acid were used to develop plates.

(9) Supramolecular Peptide Self-Assemblies Facilitate Oral ImmunizationACS biomaterials science & engineeringMay 13, 2024Curvino EJ, Woodruff ME, Roe EF, Freire Haddad H, Cordero Alvarado P, Collier JH.antigen-specific antibodies were detected using HRP-conjugated goat anti-mouse antibodies (Southern Biotech, 5300-05B). Antibody titers were determined using an absorbance value of 0.2 OD above background where titer 2

(10) Engaging natural antibody responses for the treatment of inflammatory bowel disease via phosphorylcholine-presenting nanofibresNature biomedical engineeringMay 1, 2024Curvino EJ, Roe EF, Freire Haddad H, Anderson AR, Woodruff ME, Votaw NL, Segura T, Hale LP, Collier JH.goat anti-mouse antibodies at a 1:5000 dilution in 1% BSA in 1X PBST (IgG1, IgG2b, IgG2c, IgG3, & IgM: 5300-05B). Absorbance values were then measured using a plate reader with SoftMax Pro 6.5.1 software. Area under

(11) The ZFP36 family of RNA binding proteins regulates homeostatic and autoreactive T cell responsesScience immunologyOctober 28, 2022Cook ME, Bradstreet TR, Webber AM, Kim J, Santeford A, Harris KM, Murphy MK, Tran J, Abdalla NM, Schwarzkopf EA, et al.were coated with isotype-specific capture antibodies from the SBA Clonotyping System (SouthernBiotech 5300-05B) in carbonate buffer (34.88 mM NaHCO3, 15.00 mM Na2CO3, pH 9.7) at 4 °C overnight. Following blocking

(12) Preclinical Enzyme Replacement Therapy with a Recombinant β-Galactosidase-Lectin Fusion for CNS Delivery and Treatment of GM1-GangliosidosisCellsAugust 19, 2022Jason Andrew Weesner et al.by ELISA using the SBA Clonotyping System-C57BL/6-HRP (Southern Biotech, Birmingham, AL, USA, Cat#: 5300-05B) following the manufactured instructions. Briefly, plates were coated overnight with 2.5 μg/mL of mβ-Gal:RTB

(13) NFAM1 Promotes Pro-Inflammatory Cytokine Production in Mouse and Human MonocytesFrontiers in immunologyJanuary 1, 2021Juchem KW, Gounder AP, Gao JP, Seccareccia E, Yeddula N, Huffmaster NJ, Côté-Martin A, Fogal SE, Souza D, Wang SS, et al.ELISAs were performed on NP(23)-BSA coated plates using SBA Clonotyping System-C57BL/6-HRP (Catalog #5300-05B; Southern Biotech) according to manufacturer’s recommendations. The SBA Clonotyping System-C57BL/6-HRP

(14) A mouse model of Citrobacter rodentium oral infection and evaluation of innate and adaptive immune responsesSTAR protocolsDecember 18, 2020Wang W, Li Y, Guo X.Pierce BCA protein assay kit,Thermo Fisher Scientific,Cat# 23225 SBA Clonotyping System-B6/C57J-HRP,SouthernBiotech,Cat# 5300-05B ,, Experimental models: mouse model,Experimental models: mouse model,Experimental models: mouse model ,, C57BL/6

(15) Cell-Penetrating Nanoparticles Activate the Inflammasome to Enhance Antibody Production by Targeting Microtubule-Associated Protein 1-Light Chain 3 for DegradationACS NanoMarch 24, 2020Motao Zhu, Libo Du, Ruifang Zhao, Helen Y. Wang, Yuliang Zhao, Guangjun Nie, Rong-Fu Wangmeasured using HRP-conjugated anti-mouse (C57BL/6) IgG (H+L) and IgG1 antibodies from Southern Biotech (5300–05B). In Vitro LC3-Au4.5 Binding. To check whether LC3 could directly bind to Au4.5 nanoparticles, 500

(16) Sensing Microbial Viability through Bacterial RNA Augments T Follicular Helper Cell and Antibody ResponsesImmunityMarch 20, 2018Barbet G, Sander LE, Geswell M, Leonardi I, Cerutti A, Iliev I, Blander JM.,Thermo Fisher,Cat#10296028 Brefeldin A,Sigma,Cat#B7651-5MG SBA Clonotyping System C57BL/6-HRP,SouthernBiotech,Cat#5300-05B Maxima SYBR Green/ROX qPCR Master Mix (2X),Thermo Fisher,Cat#K0222 Deposited Data,Deposited Data,Deposited

(17) New adenovirus-based vaccine vectors targeting Pfs25 elicit antibodies that inhibit Plasmodium falciparum transmissionMalaria JournalDecember 1, 2017Kathleen A. McGuire, Kazutoyo Miura, Christopher M. Wiethoff, Kim C. Williamsonsecondary antibodies (1:500 dilution) added were specific to IgG1, IgG2a or IgG3 (Southern Biotech, cat # 5300-05B, Birmingham, AL, USA). Determining the relative avidity of Pfs25-specific serum antibodies The avidity

(18) Follicular Helper T Cells are Essential for the Elimination of Plasmodium InfectionEBioMedicineOctober 1, 2017Pérez-Mazliah D, Nguyen MP, Hosking C, McLaughlin S, Lewis MD, Tumwine I, Levy P, Langhorne J.described (Quin and Langhorne, 2001), with the SBA Clonotyping System-C57BL/6-HRP (SouthernBiotech Cat# 5300-05B). A pool of plasma from NIMR C57BL/6 mice challenged at least six times with P. chabaudi was used as

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SBA Clonotyping System-C57BL/6-HRP from SouthernBiotech

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SBA Clonotyping System-C57BL/6-HRP from SouthernBiotech

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