SBA Clonotyping System-HRP 5300-05 from SouthernBiotech

Product Specs
ItemSBA Clonotyping System-HRP
CompanySouthernBiotech
Price $480.00Supplier Page View Company Product Page
Catalog Number5300-05
Quantity1 kit
ApplicationsELISA
Conjugate/Tag/LabelHRP (Horseradish Peroxidase)
FormatLiquid - UNLB antibody - Borate buffered saline, pH 8.2 HRP conjugates - 50% Glycerol/50% Phosphate buffered saline, pH 7.4
Research AreaImmunology, Secondary Antibodies
HostGoat

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SouthernBiotech

160 Oxmoor Boulevard

Birmingham, Alabama 35209

United States

Phone: (205) 945-1774
Toll-Free U.S. and Canada: (800) 722-2255
Fax: (205) 945-8768

Company Profile

Website: http://www.southernbiotech.com

(1) Immunogenicity of a Recombinant MVA Vector Vaccine Expressing the Prefusion RSV F Protein in Balb/c MiceVaccines (Basel)March 31, 2026Jinhui Miao, Min Liu, Qun Zhang, Feixia Gao, Yongshan Zheng, Cheng Hewith HRP-conjugated goat anti-mouse IgG1 or IgG2a secondary antibody (SouthernBiotech, Beijing, China, 5300-05) at 37 °C for 1 h. After a final wash step, TMB substrate (InnoReagents, Deqing, China, TMB-S-001) was

(2) Induction of Autoimmune Myocarditis in Diversity Outbred MiceBiology (Basel)February 6, 2026Meghna Sur, Kiruthiga Mone, Shraddha Singh, Mahima T. Rasquinha, Jean-Jack M. Riethoven, Indranil Mukhopadhyay, Raymond A. Sobel, Jay Reddyhorseradish peroxide (HRP)-labeled goat anti-mouse total Ig, IgM, IgG1, IgG2a, IgG2b, and IgG3 (cat. no. 5300-05), as well as IgG2c (cat. no. 1079-05), diluted 1:500 in assay buffer, were added as secondary antibodies

(3) Deletion of Ptpn2 in B cells promotes autoimmunity via TLR and JAK/STAT signalingJCI InsightDecember 22, 2025Bridget N. Alexander, Soojin Kim, Kristen L. Wells, Maya J. Hunter, Kevin P. Toole, Scott M. Wemlinger, Daniel P. Regan, Andrew Getahun, Mia J. Smithanalysis. Serum ELISA. Microtiter plates were coated with 10 μg/mL goat anti-mouse Ig (Southern Biotech, 5300-05), 10 μg/mL recombinant human insulin (Sigma-Aldrich), or 10 μg/mL calf thymus DNA (Invitrogen) in PBS

(4) ST8Sia6 overexpression protects pancreatic β cells from spontaneous autoimmune diabetes in nonobese diabetic miceJ Clin InvestAugust 1, 2025Justin Choe, Paul Belmonte, Sydney Crotts, Thanh Nguyen, David Friedman, Alexi Zastrow, Matthew Rajcula, Brady Hammer, Claire Wilhelm, Michael J. Shapiro, et al.PBS plus 0.005% Tween-20, then incubated with HRP-conjugated goat anti-mouse IgG (Southern Biotech 5300-05) at 1:1,000 in PBS plus 0.05% BSA for 1 hour at room temperature on a shaker. Wells were washed 10 times

(5) ST8Sia6 overexpression protects pancreatic β cells from spontaneous autoimmune diabetes in nonobese diabetic miceJ Clin InvestAugust 1, 2025Justin Choe, Paul Belmonte, Sydney Crotts, Thanh Nguyen, David Friedman, Alexi Zastrow, Matthew Rajcula, Brady Hammer, Claire Wilhelm, Michael J. Shapiro, et al.PBS plus 0.005% Tween-20, then incubated with HRP-conjugated goat anti-mouse IgG (Southern Biotech 5300-05) at 1:1,000 in PBS plus 0.05% BSA for 1 hour at room temperature on a shaker. Wells were washed 10 times

(6) Design of a Multi-Epitope Vaccine Based on Fasciola gigantica Cathepsin B and Evaluation of Immunological Responses in MiceInt J Mol SciJuly 20, 2025Supanan Chansap, Werachon Cheukamud, Thitikul Suthisintong, Pornanan Kueakhai, Narin Changklungmoaperoxidase (HRP)-conjugated goat anti-mouse IgG1 or IgG2a (Southern Biotech, Birmingham, AL, USA, Cat. No. 5300-05) diluted with PBS at a ratio of 1:500. After five washes with 0.05% PBST, 50 μL of 3, 3′, 5, 5′-tetramethylbenzidine

(7) Development of multi-epitope Cathepsin L driven short peptide vaccine against Fasciola giganticaFront Vet SciMay 22, 2025Supanan Chansap, Werachon Cheukamud, Thitikul Suthisintong, Pornanan Kueakhai, Narin Changklungmoa(HRP)-conjugated with goat anti-mouse IgG1 or IgG2a (Southern Biotech, Birmingham, AL, United States, Cat. No. 5300–05) diluted with PBS at 1:500 to incubated for 2 h at RT. The plates were washed with 0.05% PBST five times,

(8) Deletion of Card9 eliminates the detrimental facets of mycobacterial adjuvantsHeliyonOctober 15, 2024Hideo Mitsuyama, Ei'ichi Iizasa, Akiko Kukita, Shuji Toda, Hiroki Yoshida, Hiromasa Inoue, Hiromitsu Haraperoxidase-conjugated goat anti-mouse IgM, IgG1, and IgG2b antibodies were purchased from Southern Biotech (5300-05). Collagenase Type 4 was obtained from Worthington (CLS-4). ELISA kits for mouse IL-6 (43135), mouse

(9) A Nanobody/Monoclonal Antibody “hybrid” sandwich technology offers an improved immunoassay strategy for detection of African trypanosome infectionsPLOS Neglected Tropical DiseasesJuly 1, 2024Steven Odongo, Bo-Kyung Jin, Hang Thi Thu Nguyen, Magdalena Radwanska, Stefan Magezimmunoglobulin (Ig) horseradish peroxidase (HRP) (SouthernBiotech SBA Clonotyping System-HRP, Cat. No. 5300–05) diluted (1/1000) in 2.5% milk solution was added into the wells (50 μl/well) followed by incubation

(10) A Novel Class of Human ADAM8 Inhibitory Antibodies for Treatment of Triple-Negative Breast CancerPharmaceuticsApril 13, 2024Mineva ND, Pianetti S, Das SG, Srinivasan S, Billiald NM, Sonenshein GE.purity (SDS-PAGE). Isotype and light chain type were determined using an SBA Clonotyping System-HRP kit (5300-05, Southern Biotech, Birmingham, AL, USA). 2.3. ELISA Briefly, 96-well plates, coated with 1 μg/mL rHuADAM8

(11) Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus CD2v and p54International journal of molecular sciencesDecember 26, 2023Wei J, Liu C, He X, Abbas B, Chen Q, Li Z, Feng Z.using an enzyme-linked immunosorbent assay (ELISA) (Southern Biotech, Birmingham, AL, USA, 5300-01 and 5300-05). The safety of the recombinant virus was also evaluated by measuring IL-6 in serum after viral infection.

(12) Immunization of BALB/c Mice with Killed Tachyzoites of Toxoplasma gondii against Acute ToxoplasmosisIranian Journal of ParasitologyDecember 1, 2023Hai-Ting Guo, Jie Tan, Yu-Lin He, Xia Li, Xue Liang, Zhong-Yuan LiBirmingham, USA) based on the manufacturer’s guidance (https://resources.southernbiotech.com/techbul/5300-05.pdf). The optical density of each well was recorded at 450 nm at 10 min after substrate addition by

(13) miR-15/16 clusters restrict effector Treg cell differentiation and functionJournal of Experimental MedicineOctober 2, 2023Jiayi Dong, William J. Huth, Nimi Marcel, Ziyue Zhang, Ling-Li Lin, Li-Fan LuTween-20 in PBS) three times. Bound antibodies were detected by HRP-conjugated anti-mouse IgG1 antibodies (5300-05; 1:1,000; Southern Biotech). The reactions were developed by incubation for 15 min at room temperature

(14) Intranasal boosting with RBD-HR protein vaccine elicits robust mucosal and systemic immune responsesGenes & DiseasesAugust 3, 2023Li Chen, Wenyan Ren, Hong Lei, Jiayu Wang, Haiying Que, Dandan Wan, Aqu Alu, Dandan Peng, Minyang Fu, Weiqi Hong, et al.subtypes and IgA detection, goat anti-mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, and IgA secondary antibodies (5300-05, Southern Biotech, USA; ab97255, Abcam, UK) were used at a dilution of 1:10,000. Endpoint titers were

(15) The extracellular matrix of lymph node reticular fibers modulates follicle border interactions and germinal center formationiScienceMay 19, 2023Song J, Deshpande T, Zhang X, Hannocks MJ, Lycke N, Cardell SL, Sorokin L.assays,Critical commercial assays,Critical commercial assays ,, Clonotyping System Kit,Southern Biotech,Cat # 5300-05 EasySep Mouse B-cell isolation kit,Stem Cell Technologies,Cat # 19854A RNAscope® Multiplex Fluorescent

(16) A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 InteractomesMolecular & cellular proteomics : MCPNovember 1, 2022Song DA, Alber S, Doron-Mandel E, Schmid V, Albus CA, Leitner O, Hamawi H, Oses-Prieto JA, Dezorella N, Burlingame AL, et al.isotyping kit, according to the manufacturer’s instructions (SBA Clonotyping System-HRP, SouthernBiotech, 5300-05). Monoclonal antibodies were purified by Protein A affinity purification, using an AKTA PURE system.

(17) Influenza Virus-like Particle-Based Hybrid Vaccine Containing RBD Induces Immunity against Influenza and SARS-CoV-2 VirusesVaccinesJune 14, 2022Bommireddy R, Stone S, Bhatnagar N, Kumari P, Munoz LE, Oh J, Kim KH, Berry JTL, Jacobsen KM, Jaafar L, et al.ThermoFisher Scientific/Pierce (Cat#31436). The antibody isotyping kit was purchased from Southern Biotech (Cat#5300-05). FITC-conjugated streptavidin was purchased from BD Biosciences (Cat#554060). Human COVID-19 convalescent

(18) The ASCIZ-DYNLL1 Axis Is Essential for TLR4-Mediated Antibody Responses and NF-κB Pathway ActivationMolecular and cellular biologyNovember 22, 2021Liu R, King A, Tarlinton D, Heierhorst J.horseradish peroxidase (HRP)‐labeled goat anti-mouse IgM, IgG1, IgG2, or IgG3 antibodies (Southern Biotech, 5300-05). For quantification, reference wells were coated with goat anti-mouse Ig capture antibody and purified

(19) An AAV-based, room-temperature-stable, single-dose COVID-19 vaccine provides durable immunogenicity and protection in non-human primatesCell host & microbeSeptember 8, 2021Zabaleta N, Dai W, Bhatt U, Hérate C, Maisonnasse P, Chichester JA, Sanmiguel J, Estelien R, Michalson KT, Diop C, et al.performed but using the secondary antibodies from SBA Clonotyping System-HRP kit (SouthernBiotech, 5300-05, RRID:AB_2796080) diluted accordingly to manufacturer’s instructions. Cynomolgus macaque samples were

(20) The History of Anti-Trypanosome Vaccine Development Shows That Highly Immunogenic and Exposed Pathogen-Derived Antigens Are Not Necessarily Good Target Candidates: Enolase and ISG75 as ExamplesPathogensAugust 19, 2021Stefan Magez, Zeng Li, Hang Thi Thu Nguyen, Joar Esteban Pinto Torres, Pieter Van Wielendaele, Magdalena Radwanska, Jakub Began, Sebastian Zoll, Yann G.-J. Sterckxperoxidase-labeled specific secondary antibodies (Southern Biotech, Birmingham, AL, USA, kit Cat. No. 5300-05). TMB (Sigma, St. Louis, MO, USA Cat. No. T0440) substrate conversion was measured at 450 nm (with 570

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SBA Clonotyping System-HRP from SouthernBiotech

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SBA Clonotyping System-HRP from SouthernBiotech

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