cAMP Assay Kit (Competitive ELISA, Fluorometric) ab138880 from Abcam

Product Specs
ItemcAMP Assay Kit (Competitive ELISA, Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab138880
Sample TypeUrine, Plasma, Tissue Extracts, Cell Lysate, Cell culture media
SensitivityEqual to 1 nM
Assay Time3h 0m
ApplicationsELISA
Quantity1 x 96 Tests, 10 x 96 Tests

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Website: http://www.abcam.com

(1) HHEX–PRKAR2B Axis-Mediated PKA Activation Drives Glucose Metabolism-Dependent Progression of Pancreatic Ductal AdenocarcinomaiScienceFebruary 20, 2026Junxiang Wen, Qiuchen Li, Shuxiang Xu, Wenjun Lu, Fei Wu, Jiatao Lou, Lin Wangmicroplate reader. cAMP assay The level of cAMP was assessed using a cAMP Direct Immunoassay Kit (cat. ab138880, Abcam). Cells were grown in a sterile 96-well plate and incubated overnight in a 37°C/5% CO2 incubator,

(2) Hydrogen Sulfide Deficiency Contributes to Tubular Damage and Calcium Oxalate Crystal Formation in Hyperoxaluria Nephropathy: Role of Osteopontin and Tamm–Horsfall ProteinAntioxidants (Basel)September 5, 2025Chien-Lin Lu, Yi-Shiou Tseng, Wen-Bin Wu, Chun-Hou Liao, Ming-Chieh Maand PKA Activity The cAMP levels and PKA activity were assessed using a commercial kinase assay kit (ab138880 and ab139435, respectively, Abcam, Cambridge, UK) in accordance with the manufacturer’s protocol. MDCK cells

(3) Adenylate cyclase 10 promotes brown adipose tissue thermogenesisiScienceFebruary 21, 2025Anupam Das, Christine Mund, Eman Hagag, Ruben Garcia-Martin, Eleftheria Karadima, Anke Witt, Mirko Peitzsch, Andreas Deussen, Triantafyllos Chavakis, Thomas Noll, et al.dye,Sigma-Aldrich,1024190250 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, cAMP Assay kit,Abcam,ab138880 IDH Assay kit,Abcam,ab102528 OGDH Activity Assay kit,Biomol,G-MAES0236.96 Seahorse XF Cell Mito Stress Test Kit

(4) SIRT6 Ameliorates Cancer Cachexia-Associated Adipose Wasting by Suppressing TNFR2 Signalling in MiceJ Cachexia Sarcopenia MuscleFebruary 19, 2025Kang Xu, Yida Wang, Fang Wang, Yannan Guo, Yu Ren, Vivien Low, Sungyun Cho, Qingfei Liu, Ying Qiu, Xue Li, et al.Solarbio). 2.6 cAMP Levels Analysis cAMP (cyclic AMP) concentrations were quantified by cAMP Assay Kit (ab138880, Abcam) according to the instructions. The fluorescence was measure at λex = 540 nm and λem = 590 nm

(5) Biochanin a modulates steroidogenesis and cellular metabolism in human granulosa cells through TAS2Rs activation: a spotlight on ovarian functionReprod Biol EndocrinolJanuary 25, 2025Francesca Paola Luongo, Sofia Passaponti, Alesandro Haxhiu, Irene Ortega Baño, Rosetta Ponchia, Giuseppe Morgante, Paola Piomboni, Alice Luddiproduction (nM) in the samples was assessed using an ELISA cAMP Direct Immunoassay Detection Kit (Abcam, ab138880) according to the manufacturer’s instructions. ELISA plates were analyzed using a Synergy MX plate reader

(6) GZMA suppressed GPX4-mediated ferroptosis to improve intestinal mucosal barrier function in inflammatory bowel diseaseCell Communication and SignalingOctober 4, 2024Rongwei Niu, Jiaoli Lan, Danxia Liang, Li Xiang, Jiaxin Wu, Xiaoyan Zhang, Zhiling Li, Huan Chen, Lanlan Geng, Wanfu Xu, et al.1:1000 for IF), CD39(ab300065, 1:250 for IF), Human Granzyme A ELISA Kit (ab255728) and cAMP ELISA Kit(ab138880) were purchased from Abcam (UK). Antibodies targeting GPX4(67763-1-Ig, 1:400 for IF, 1:2000 for WB),

(7) Carcinoembryonic antigen potentiates non-small cell lung cancer progression via PKA-PGC-1ɑ axisMolecular BiomedicineMay 24, 2024Juan Lei, Lei Wu, Nan Zhang, Xudong Liu, Jiangang Zhang, Liwen Kuang, Jiongming Chen, Yijiao Chen, Dairong Li, Yongsheng Litransfection. ELISA Intracellular cAMP expression in A549 cells were measured using a cAMP ELISA kit (Cat No. ab138880, Abcam) according to the manufacturer’s instruction. Immunohistochemistry Formalin-fixed paraffin-embedded

(8) Dimerization of melanocortin 4 receptor controls puberty onset and body size polymorphismFront Endocrinol (Lausanne)November 10, 2023Ruiqi Liu, Mike Friedrich, Katherina Hemmen, Kerstin Jansen, Mateus C. Adolfi, Manfred Schartl, Katrin G. HeinzecAMP response of selected constructs was monitored using cAMP Direct Immunoassays Kit (Fluorometric) (ab138880, Abcam, Cambridge, United Kingdom). HEK293T cells were seeded at 50 000 cells/well in a poly-D-lysine

(9) IL-6 translation is a therapeutic target of human cytokine release syndromeJournal of Experimental MedicineNovember 6, 2023Yuzhuo Yang, Yajing Zhang, Xiaoyan Xing, Gang Xu, Xin Lin, Yao Wang, Meixia Chen, Chunmeng Wang, Bin Zhang, Weidong Han, et al.were lysed by cell lysis buffer provided by the cAMP Direct Immunoassay Detection Kit (Fluorometric; ab138880; Abcam), and cAMP was measured according to the manufacturer’s instructions. Western blotting Cells

(10) Calcium and bicarbonate signaling pathways have pivotal, resonating roles in matching ATP production to demandeLifeJune 5, 2023Greiser M, Karbowski M, Kaplan AD, Coleman AK, Verhoeven N, Mannella CA, Lederer WJ, Boyman L.isolated cardiac mitochondria (0.5 mg per sample) using cAMP Direct Immunoassay Kit (Fluorometric) (Abcam, ab138880). Mitochondria samples were treated with 0 or 10 or 15 mM [HCO3−], and 0 or 25 μM forskolin for 30 min,

(11) Alkaline Dilution Alters Sperm Motility in Dairy Goat by Affecting sAC/cAMP/PKA Pathway ActivityInternational Journal of Molecular SciencesJanuary 16, 2023Qifu He, Feng Gao, Shenghui Wu, Shaowen Wang, Zhiming Xu, Xuerui Xu, Tianyang Lan, Kang Zhang, Fusheng Quandescribed by Jaiswal and Conti [64]. For the cAMP evaluation, a cAMP Direct Immunoassay Fluorometric Kit (ab138880, Abcam, Cambridge, United Kingdom) was used, following the manufacturer’s specifications. Fluorescence

(12) PAQR8 promotes breast cancer recurrence and confers resistance to multiple therapiesBreast Cancer ResearchJanuary 3, 2023Saisai Chen, Matt R. Paul, Christopher J. Sterner, George K. Belka, Dezhen Wang, Peining Xu, Amulya Sreekumar, Tien-chi Pan, Dhruv K. Pant, Igor Makhlin, et al.(#926-32219). cAMP Intracellular cAMP levels were measured using a fluorometric competitive ELISA assay kit (Abcam #ab138880) following manufacturer’s instructions. Liquid chromatography-high resolution mass spectrometry Sphingolipid

(13) Autophagy regulates neuronal excitability by controlling cAMP/protein kinase A signaling at the synapseThe EMBO journalNovember 17, 2022Overhoff M, Tellkamp F, Hess S, Tolve M, Tutas J, Faerfers M, Ickert L, Mohammadi M, De Bruyckere E, Kallergi E, et al.autophagy‐deficient mice was measured using the cAMP Direct Immunoassays Kit (Flourometric) from Abcam (ab138880) following the manufacturers' instructions. Hippocampi of Atg5flox:CamKIIα‐Cre WT and KO mice were dissected

(14) Lysophosphatidylcholine (17:0) Improves HFD-Induced Hyperglycemia & Insulin Resistance: A Mechanistic Mice Model StudyDiabetes Metab Syndr ObesNovember 15, 2022Li Bao, Ying Zhang, Sichao Yan, Dan Yan, Dechun Jiangmanufacturer’s instructions. Cyclic adenosine monophosphate (cAMP) was measured using a cAMP Assay Kit (ab138880, Abcam, USA). Insulin Tolerance Test (ITT) and Oral Glucose Tolerance Test (OGTT) ITT was performed

(15) Hepatic thyroid hormone signalling modulates glucose homeostasis through the regulation of GLP-1 production via bile acid-mediated FXR antagonismNature CommunicationsOctober 27, 2022Ying Yan, Zhoumin Niu, Chao Sun, Peng Li, Siyi Shen, Shengnan Liu, Yuting Wu, Chuyu Yun, Tingying Jiao, Sheng Jia, et al.extracted from frozen intestine tissue samples by lysis buffer and analyzed by a mouse cAMP Assay kit (ab138880, Abcam). Intracellular cAMP was extracted from STC-1, NCI-H716 cells and intestinal organoids, and then

(16) Phosphodiesterase 4D contributes to angiotensin II-induced abdominal aortic aneurysm through smooth muscle cell apoptosisExperimental & Molecular MedicineAugust 23, 2022Ran Gao et al.(Supplementary Fig. 12a–d). Measurement of cAMP A cAMP Direct Fluorometric Immunoassays Kit (Abcam, ab138880) was used to measure the cAMP level according to the manufacturer’s instructions. For in vitro cAMP

(17) Conjugated secondary 12α-hydroxylated bile acids promote liver fibrogenesisEBioMedicineApril 1, 2021Xie G, Jiang R, Wang X, Liu P, Zhao A, Wu Y, Huang F, Liu Z, Rajani C, Zheng X, et al.cAMP was extracted, and determined using a cAMP Assay Kit according to the manufacturer's instruction (ab138880, Abcam). Cell proliferation assays Number of liver cells was determined using a WST-8-based Cell Counting

(18) Novel curcumin analog (cis-trans curcumin) as ligand to adenosine receptors A2A and A2B: potential for therapeuticsPharmacological ResearchMarch 1, 2021Luke J. Hamilton, Michaela Walker, Mahesh Pattabiraman, Haizhen A. Zhong, Brandon Luedtke, Surabhi Chandraincubate for 2h. The rest of the experiment was performed according to the manufacturer’s protocol (ab138880 cAMP Direct Immunoassay Kit, Abcam, Cambridge, UK). The plate was read with a Synergy H1 microplate

(19) Protective Effects of Oroxylin A against Doxorubicin-Induced Cardiotoxicity via the Activation of Sirt1 in MiceOxidative Medicine and Cellular LongevityJanuary 19, 2021Wen-Bin Zhang, Yong-Fa Zheng, Yao-Gui Wu(colorimetric, ab65348), protein kinase A (PKA, ab139435) activity, and cAMP level (competitive ELISA, ab138880) were provided by Abcam (Cambridge, UK). 2.2. Animals and Treatment. All animals’ experimental protocols

(20) Adaptive Thermogenesis in Mice Is Enhanced by Opsin 3-Dependent Adipocyte Light SensingCell reportsJanuary 21, 2020Nayak G, Zhang KX, Vemaraju S, Odaka Y, Buhr ED, Holt-Jones A, Kernodle S, Smith AN, Upton BA, D'Souza S, et al.cells were then harvested to quantify cAMP levels by direct immunoassay (fluorometric kit by Abcam, ab138880) as per manufacturer’s instructions. For ex vivo cAMP quantification from harvested tissue, dissected

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cAMP Assay Kit (Competitive ELISA, Fluorometric) from Abcam

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cAMP Assay Kit (Competitive ELISA, Fluorometric) from Abcam

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