Hydrogen Peroxide Assay Kit (Cell-based) ab138874 from Abcam

Product Specs
ItemHydrogen Peroxide Assay Kit (Cell-based)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab138874
Sample TypeAdherent cells, Suspension cells
SensitivityEqual to 3 µM
Assay Time0h 30m
Research AreaMetabolism
Quantity200 Test

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Cambridge CB2 0AX

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Synergistic Induction of Oxidative and Endoplasmic Reticulum Stress by Tempol and ML210 Combination Therapy in B16F10 Melanoma CellsInt J Mol SciMarch 14, 2026Ebru Çelik, Percin Pazarci, Ömer Kokaçya, Halil Mahir KaplanLevels Intracellular H2O2 levels were determined using an intracellular H2O2 assay kit (Abcam, cat # ab138874, Waltham, MA, USA). Known concentrations of standard H2O2 provided in the kit were utilized to generate

(2) Involvement of endolysosome iron in HIV-1 gp120-, morphine-, and iron supplementation-induced disruption of the reactive species interactome and induction of neurotoxicityRedox RepAugust 21, 2025Nirmal Kumar, Peter W. Halcrow, Darius N. K. Quansah, Braelyn Liang, Olimpia Meucci, Jonathan D. GeigerFe2+, CM-H2DCFDA (Invitrogen, Cat. #C6827) for ROS, H2O2 assay kit (Abcam, Boston, MA, U.S.A., Cat. #ab138874) for H2O2, and SF7-AM for H2S. SH-SY5Y Cells (3–6 × 105 cells/well) were added to 24-well plates and

(3) Current understanding of eryptosis: mechanisms, physiological functions, role in disease, pharmacological applications, and nomenclature recommendationsCell Death DisJuly 1, 2025Anton Tkachenko, Mohammad A. Alfhili, Jawaher Alsughayyir, Alessandro Attanzio, Abdulla Al Mamun Bhuyan, Bożena Bukowska, Antonio Cilla, Martha A. Quintanar-Escorza, Michael Föller, Ondrej Havranek, et al.staining).ii.Specific ROS (colorimetric or fluorogenic substrate-based methods):Hydrogen peroxide (H2O2) – Abcam #ab138874; Amplex Red.Superoxide anion (O2-) – Abcam #ab139477 (FCM).Hydroxyl radicals (OH-) – Abcam #ab219931

(4) Mesoporous polydopamine@CeO2 nanoparticles for photoacoustic-guided photothermal therapy in suppressing arthritisiScienceMay 3, 2025Huazhen Liu, Jingjing Wang, Chenyang Zhao, Rui Zhang, Ming Wang, Yuxin Jiang, Desheng Sun, Yanglong Hou, Meng Yangkilling inflammatory cells. Intracellular H2O2 decomposing We used a hydrogen peroxide assay kit (ab138874, Abcam, Sigma-Aldrich) to measure the H2O2 decomposing efficiency of MPDA@CeO2 NPs. Firstly, we added

(5) Cobalt ions induce a cellular senescence secretory phenotype in human synovial fibroblast-like cells that may be an early event in the development of adverse local tissue reactions to hip implantsOsteoarthritis and Cartilage OpenSeptember 1, 2024Michael P. Grant, Raheef Alatassi, Mohamad Omar Diab, Mohammed Abushal, Laura M. Epure, Olga L. Huk, Stephane G. Bergeron, Hee-Jeong Im Sampen, John Antoniou, Fackson Mwalewas performed using a Hydrogen Peroxide Assay kit following manufacturer's guideline (Abcam, Cat. #ab138874). Cells (5000/well) were seeded on Lab-Tek chamber slides (ThermoFisher Scientific, Cat. #ab138874)

(6) Effects of Cannabidiol, ∆9-Tetrahydrocannabinol, and WIN 55-212-22 on the Viability of Canine and Human Non-Hodgkin Lymphoma Cell LinesBiomoleculesApril 19, 2024Saba Omer, Suhrud Pathak, Mahmoud Mansour, Rishi Nadar, Dylan Bowen, Muralikrishnan Dhanasekaran, Satyanarayana R. Pondugula, Dawn Boothetreated 1771 canine B-cell lymphoma cells was measured using the Abcam Hydrogen Peroxide Assay kit (ab138874). A standard curve for H2O2 was obtained using a fluorometric plate reader (BioTek Synergy HT plate

(7) Agonists and hydrogen peroxide mediate hyperoxidation of β2-adrenergic receptor in airway epithelial cells: Implications for tachyphylaxis to β2-agonists in constrictive airway disordersBiomedicine & PharmacotherapyDecember 1, 2023Kirti Singh, Razan L. Teyani, Nader H. MoniriH2O2 detection reagents were purchased from Thermo Fisher Scientific (A12222) (Waltham, MA) and Abcam (ab138874) (Waltham, MA), respectively. DiaAlk (PubChem CID: 134688955) (1-(tert-Butyl) 2-(2-methyl-4-(prop-2-yn-1-yloxy)

(8) PLAC8-Mediated Activation of NOX4 Signalling Restores Angiogenic Function of Endothelial Colony-Forming Cells in Experimental HypoxiaCellsSeptember 6, 2023Pun SH, O'Neill KM, Edgar KS, Gill EK, Moez A, Naderi-Meshkin H, Malla SB, Hookham MB, Alsaggaf M, Madishetti VV, et al.superoxide, while hydrogen peroxide production was quantified using a commercially available assay kit (ab138874, Abcam), according to the manufacturer’s instructions. For superoxide assay, cells were seeded on a

(9) Soluble epoxide hydrolase maintains steady-state lipid turnover linked with autocrine signaling in peritoneal macrophagesiScienceAugust 18, 2023Feng Liu, Xueying Diao, Haolun Cong, Eriko Suzuki, Keiji Hasumi, Hiroshi TakeshimaLtd.,CH5-24 "Latex beads, fluorescent red",Sigma-Aldrich,L3030 Hydrogen peroxide assay kit (Cell-based),Abcam,ab138874 Mouse TNF-α quantikine ELISA Kit,R&D Systems,MTA00B ,, Deposited data,Deposited data,Deposited data ,, Raw

(10) Rebalance of the Polyamine Metabolism Suppresses Oxidative Stress and Delays Senescence in Nucleus Pulposus CellsOxidative Medicine and Cellular LongevityFebruary 7, 2022Hui Che, Cheng Ma, He Li, Fenglei Yu, Yifan Wei, Hailong Chen, Jun Wu, Yongxin Ren(ab48508), anti-gamma H2A.X (ab81299), IgG H&L Alexa Fluor 488 (ab150113), Hydrogen Peroxide Assay Kit (ab138874, ab102500), and Total Polyamine Assay Kit (ab239728) were from Abcam (Cambridge, USA); Trizol reagent

(11) Tanshinone‑IIA inhibits myocardial infarct via decreasing of the mitochondrial apoptotic signaling pathway in myocardiocytesInternational Journal of Molecular MedicineJune 29, 2021Yeqing Fang, Chengcheng Duan, Shaoyuan Chen, Zhenguo Liu, Bimei Jiang, Wen Ai, Lei Wang, Peiyi Xie, Hongcheng Fang1:1,000; ab118970; Abcam), reactive oxygen species (ROS; 1:1,000; ab186027; Abcam), H2O2 (1:1,000; ab138874; Abcam) overnight at 4°C. Myocardiocytes were washed with PBS and incubated with corresponding anti-rabbit

(12) Arginase-II promotes melanoma migration and adhesion through enhancing hydrogen peroxide production and STAT3 signalingJournal of cellular physiologyDecember 1, 2020Yu Y, Ladeiras D, Xiong Y, Boligan KF, Liang X, von Gunten S, Hunger RE, Ming XF, Yang Z.Hydrogen Per- Ninety percent of confluent melanoma cell layers were wounded by oxide Assay Kit (Abcam, ab138874), respectively, according to the scratching with a sterile 200 μl pipette tip. Then, the cells continued manufacturers'

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Hydrogen Peroxide Assay Kit (Cell-based) from Abcam

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Hydrogen Peroxide Assay Kit (Cell-based) from Abcam

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