FLIPR® Membrane Potential (Blue) Assay Kit R8042 / R8034 from Molecular Devices

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ItemFLIPR® Membrane Potential (Blue) Assay Kit
CompanyMolecular Devices
Price Supplier Page View Company Product Page
Catalog NumberR8042 / R8034

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Molecular Devices

3860 N First Street

San Jose, California 95134

United States

Phone: 408-747-1700

Company Profile

Website: www.moleculardevices.com

(1) Functional characterization of the bovine sour taste receptor Otopetrin 1 (OTOP1) in response to dietary and ruminal acidsBiochemistry and Biophysics ReportsMarch 1, 2026Fuminori Kawabata, Yuko Kawabataequal volume (70 μL) of membrane potential-sensitive dye solution (FLIPR membrane potential assay kit; R8042, Molecular Devices, San Jose, CA, USA) for 30 min at 37 °C. Changes in fluorescence intensity (excitation,

(2) Molecular determinant of low-voltage dependence of human Nav1.7 inactivation revealed by efficacy-based Nav1.7 selective inhibitorNature CommunicationsFebruary 10, 2026Fang Zhao, Chuchu Xi, Jie Li, Kerui Ren, Qinglian Tang, Huaduan Liang, Shilong Yang, Michael X. Zhu, Zhengyu CaoChina). FLIPR Membrane Potential Assay Kit Blue (FMPblue) dye was purchased from Molecular Devices (Cat# R8034, Sunnyvale, CA, USA). Penicillin and streptomycin (Cat# 15140148), trypsin (Cat# 25200072), soybean

(3) Precision A3G base editors for disease modeling and correctionMolecular TherapyJanuary 14, 2026Hongzhi Zeng, Aidi Liu, Tyler C. Daniel, Devin A. Golla, Zhenyu Lu, Rebecca Serodio, Brigid A. Millette, Ananya Lingineni, Peretz Gilberd, Kelly Chee, et al.with the halide-free buffer before being incubated with 100 μl of FLIPR Blue Dye (Molecular Devices, R8042) at a concentration of 0.5 mg.ml in the wash buffer for 30 minutes at 37 °C. Basal fluorescence was

(4) Inhibition of the oncogenic channel Kv10.1 by the antipsychotic drug penfluridolFrontiers in PharmacologySeptember 3, 2025Paulina León-Sánchez, Andrea Lizeth Cedillo-Hernández, María Luisa Durán-Pastén, Cesar Oliver Lara-Figueroa, Arturo Hernández-Cruz, Enoch Luis5% CO2 incubator. 2.2 High-throughput fluorescence assay The FLIPR membrane potential (FMP) assay (R8034, Molecular Devices) was performed as described by Gómez-Herrera et al. (2023) and according to the manufacturer’s

(5) Cocaine chemogenetics blunts drug-seeking by synthetic physiologyNatureAugust 27, 2025Juan L. Gomez, Christopher J. Magnus, Jordi Bonaventura, Oscar Solis, Fallon P. Curry, Marjorie R. Levinstein, Reece C. Budinich, Meghan L. Carlton, Emilya N. Ventriglia, Sherry Lam, et al.potential assay solution was prepared with a bottle of Molecular Devices membrane potential assay kit–Blue (R8034) dissolved in 200 ml of HEPES-buffered Earle’s balanced salt solution (HEBSS). Drug compound plates

(6) Open-source, three-dimensionally printed manifolds for exposure studies using human airway epithelial cellsERJ Open ResearchJune 30, 2025Ryan Singer, Nadia Milad, Elizabeth Ball, Jenny P. Nguyen, Quynh Cao, Ravi Selvaganapathy, Boyang Zhang, Mohammadhossein Dabaghi, Imran Satia, Jeremy A. Hirota(Molecular Devices, product No. i3x) and the FLIPR membrane potential assay kit (Molecular Devices, product No. R8042), following the manufacturers' instructions and using methods previously described [12, 26]. Briefly,

(7) Deleterious effect of Pseudomonas aeruginosa on F508del-CFTR rescued by elexacaftor/tezacaftor/ivacaftor is clinical strain-dependent in patient-derived nasal cellsERJ Open ResearchMay 27, 2025Caterina Allegretta, Enza Montemitro, Maria Noemi Sgobba, Valeria Capurro, Emanuela Pesce, Fabiana Ciciriello, Gianfranco La Bella, Martina Rossito, Vanessa Tuccio, Fabio Arena, et al.18]. Fluorescence-based membrane polarisation assay The cells were loaded with FLIPR blue membrane potential dye (cat #R8034; Molecular Devices) dissolved in chloride-free buffer [19]. The plate was read in a fluorescence plate

(8) Identification and development of TRPM4 antagonists to counteract neuronal excitotoxicityiScienceDecember 20, 2024Lars Binkle-Ladisch, Andy Pironet, Andrea Zaliani, Chantal Alcouffe, Daniel Mensching, Undine Haferkamp, Anne Willing, Marcel S. Woo, Alexandre Erdmann, Timm Jessen, et al.volume of 25 μl before addition of 25 μl FLIPR membrane potential (FMP) blue dye (Molecular Devices, R8034). Cells were incubated for 60 min at 37°C before placement into the FLIPR Tetra High-Throughput Cellular Screening

(9) Molecular basis of inhibition of the amino acid transporter B(0)AT1 (SLC6A19)Nature communicationsAugust 22, 2024Xu J, Hu Z, Dai L, Yadav A, Jiang Y, Bröer A, Gardiner MG, McLeod M, Yan R, Bröer S.depolarisation induced by amino acid transport was monitored using a FLIPR® kit (Molecular Devices, R8042 BLUE DYE). Before the assay, CHO-BC cells were seeded out at a density of 60,000 cells/well and maintained

(10) An in vitro assay to investigate venom neurotoxin activity on muscle-type nicotinic acetylcholine receptor activation and for the discovery of toxin-inhibitory moleculesBiochemical PharmacologyOctober 1, 2023Rohit N. Patel, Rachel H. Clare, Line Ledsgaard, Mieke Nys, Jeroen Kool, Andreas H. Laustsen, Chris Ulens, Nicholas R. Casewellunless stated otherwise. One vial of FLIPR membrane potential dye (Component A, Explorer Kit Blue, R8042, Molecular Devices, San Jose, CA, USA) was dissolved in 36 mL assay buffer to create the dye solution.

(11) Fluorescent membrane potential assay for drug screening on Kv10.1 channel: identification of BL-1249 as a channel activatorFrontiers in PharmacologyJuly 24, 2023Mirsha Aseret Gómez-Herrera, Enikar Patlán, Armando Estrada-Garrido, Arturo Hernández-Cruz, Enoch Luis5% CO2 incubator. 2.2 Fluorescence membrane potential assay FLIPR® membrane potential assay BLUE (R8034, Molecular Devices) was performed following the manufacturer’s protocols. Flat-bottom 96-well plates

(12) Electrophysiological characterization of a schistosome transient receptor potential channel activated by praziquantelInternational Journal for ParasitologyJuly 1, 2023Evgeny G. Chulkov, Oleg Palygin, Nawal A. Yahya, Sang-Kyu Park, Jonathan S. MarchantFluorescence measurements of membrane potential A fluorescent membrane potential (Em) dye (Molecular Devices, R8042) was used to measure membrane potential by detecting changes in fluorescence in real-time using a Fluorescence Imaging

(13) 3D bioprinting patient-derived induced pluripotent stem cell models of Alzheimer’s disease using a smart bioinkBioelectronic MedicineMay 24, 2023Claire Benwood, Jonathan Walters-Shumka, Kali Scheck, Stephanie M. Willerthadding Fluorescence Imaging Plate Reader Blue dye (FLIPR) Membrane Potential Assay Kit Blue (cat no. R8042, Molecular Devices, San Hose, Ca, USA) dye to the constructs at a ratio of 1:1 with the cell media (i.e.

(14) Protocol for 3D Bioprinting Mesenchymal Stem Cell-derived Neural Tissues Using a Fibrin-based BioinkBio-protocolMay 5, 2023Perez MR, Masri NZ, Walters-Shumka J, Kahale S, Willerth SM.multiple freeze/thaw cyclesFLIPR Membrane Potential Assay Kit Blue (Molecular Devices, catalog number: R8042); storage: component A: RT; component B: 4 °CPotassium chloride (KCl) (Caledon, catalog number: 5920170);

(15) Correlation of Electrophysiological and Fluorescence-Based Measurements of Modulator Efficacy in Nasal Epithelial Cultures Derived from People with Cystic FibrosisCellsApril 17, 2023Tarini N. A. Gunawardena, Zoltán Bozóky, Claire Bartlett, Hong Ouyang, Paul D. W. Eckford, Theo J. Moraes, Felix Ratjen, Tanja Gonska, Christine E. Bearmeasurement of membrane potential using a fluorescent, voltage-sensitive small molecule—the FLIPR® dye (R8034, Molecular Devices). Following the addition of the FLIPR® dye (excitation; 510–545 nm, emission; 565–625

(16) Macrocycle-stabilization of its interaction with 14-3-3 increases plasma membrane localization and activity of CFTRNature CommunicationsDecember 1, 2022Loes M. Stevers et al.growth medium was removed from the cells by inverting the plate and FMP dye (Molecular Devices, Part #R8042) including the potentiator genistein (Sigma, G6649) was added back in 70 μl of low Cl- containing buffer

(17) A bead-based GPCR phosphorylation immunoassay for high-throughput ligand profiling and GRK inhibitor screeningCommun BiolNovember 9, 2022Johanna Kaufmann, Nina Kathleen Blum, Falko Nagel, Anna Schuler, Julia Drube, Carsten Degenhart, Julian Engel, Jan Eicke Eickhoff, Pooja Dasgupta, Sebastian Fritzwanker, et al.used to wash the cells. A membrane potential dye (FLIPR Membrane Potential kit BLUE, Molecular Devices R8034) was reconstituted according to the manufacturer’s instructions. To each well, 90 μl of the HBSS/HEPES

(18) Unbiased Millivolts Assay of Mitochondrial Membrane Potential in Intact CellsMethods in Molecular BiologyJuly 22, 2022Chad A. Lerner, Akos A. GerencserStore 100 μl aliquots at −20 °C (see Notes 3 and 10). FLIPR Membrane Potential Assay Explorer Kit (#R8042, Molecular Devices), dissolve one vial in 10 ml H2O. Store at −20 °C, aliquoted for one time use size

(19) CFTR interactome mapping using the mammalian membrane two‐hybrid high‐throughput screening systemMolecular Systems BiologyFebruary 14, 2022Sang Hyun Lim et al.cocktail,Roche,11873580001 Clarity Western ECL Substrate,Bio‐Rad,1705061 Blue membrane potential dye,Molecular devices,R8034 384 well plates,Falcon,736‐2044 96 well plates,Sarstedt,83.3924 12 well plates,SPL Life Sciences,30012 PureCol®‐coated

(20) High-throughput cell-based assays for identifying antagonists of multiple smoking-associated human nicotinic acetylcholine receptor subtypesSLAS DiscoveryJanuary 21, 2022Michelle Kassner, J. Brek Eaton, Nanyun Tang, Joachim L. Petit, Nathalie Meurice, Hongwei Holly Yin, Paul WhiteakerELx405 Select CW Plate Washer. Twenty-five microliters of 0.5x FLIPR Membrane Potential dye (Molecular Devices, R8034; prepared in wash buffer) was added to the plates using a microfill. The plates were further incubated

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FLIPR® Membrane Potential (Blue) Assay Kit from Molecular Devices

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FLIPR® Membrane Potential (Blue) Assay Kit from Molecular Devices

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