CatchPoint® Cyclic-AMP Fluorescent Assay kit from Molecular Devices

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ItemCatchPoint® Cyclic-AMP Fluorescent Assay kit
CompanyMolecular Devices
Price Supplier Page View Company Product Page
Catalog NumberCatchPoint

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Molecular Devices

3860 N First Street

San Jose, California 95134

United States

Phone: 408-747-1700

Company Profile

Website: www.moleculardevices.com

(1) Identification of signaling pathways modifying human dopaminergic neuron development using a pluripotent stem cell-based high-throughput screening automated system: purinergic pathways as a proof-of-principleFrontiers in PharmacologyJune 26, 2023Claire Boissart, Marie Lasbareilles, Johana Tournois, Laure Chatrousse, Thifaine Poullion, Alexandra Benchouaplates and treated for 1 h with 10 µM C08. Cells were lysed and cAMP quantified in the lysate using the CatchPoint Cyclic-AMP Fluorescent Assay kit following the manufacturer’s instructions (Molecular Devices, CA, United States). Compound selection

(2) A cAMP signalosome in primary cilia drives gene expression and kidney cyst formationEMBO reportsAugust 3, 2022Hansen JN, Kaiser F, Leyendecker P, Stüven B, Krause JH, Derakhshandeh F, Irfan J, Sroka TJ, Preval KM, Desai PB, et al.primary cilia (see above). ELISA‐based cAMP measurements Total cAMP levels were determined using a CatchPoint™ assay (Molecular Devices, #R8089) according to the manufacturer’s instructions. mIMCD‐3 cells were

(3) Leukocyte cytokine responses in adult patients with mitochondrial DNA defectsJournal of Molecular MedicineJune 1, 2022Kalpita R. Karan, Caroline Trumpff, Marissa Cross, Kristin M. Engelstad, Anna L. Marsland, Peter J. McGuire, Michio Hirano, Martin Picardwere aspirated and washed 3 × times with 1 × wash buffer. After the final wash, 100 μL of prepared CatchPoint HRP Development Solution was added to the wells and incubated for 10 min at RT. The plates were read

(4) The IgG3 subclass of β1-adrenergic receptor autoantibodies is an endogenous biaser of β1AR signalingMolecular Biology of the CellApril 1, 2021Maradumane L. Mohan, Yuji Nagatomo, Prasenjit Prasad Saha, Sromona D. Mukherjee, Timothy Engelman, Rommel Morales, Stanley L. Hazen, W. H. Wilson Tang, Sathyamangla V. Naga Naga Prasadperformed using whole cell lysates. The assay was done using the standard manufacturer’s protocol. The Catchpoint cAMP fluorescence assay kit from Molecular Devices (San Jose, CA) was used to measure the cAMP levels

(5) Small molecule AZD4635 inhibitor of A(2A)R signaling rescues immune cell function including CD103(+) dendritic cells enhancing anti-tumor immunityJournal for immunotherapy of cancerJuly 1, 2020Borodovsky A, Barbon CM, Wang Y, Ye M, Prickett L, Chandra D, Shaw J, Deng N, Sachsenmeier K, Clarke JD, et al.was removed and cells lysed in Cell Lysis Buffer (Molecular Devices). cAMP was quantified using the CatchPoint Cyclic-AMP Fluorescent Assay Kit (Molecular Devices) following manufacturer’s instructions. Animal studies All animal studies were

(6) Nanobody-directed targeting of optogenetic tools to study signaling in the primary ciliumeLifeJune 24, 2020Hansen JN, Kaiser F, Klausen C, Stüven B, Chong R, Bönigk W, Mick DU, Möglich A, Jurisch-Yaksi N, Schmidt FI, et al.same as described above. ELISA-based cAMP measurements Total cAMP levels were determined using a CatchPoint assay (Molecular Devices) according to manufacturer’s instructions mIMCD-3, mIMCD-3 bPAC-mCherry, or

(7) A Cardiovascular Disease-Linked Gut Microbial Metabolite Acts via Adrenergic ReceptorsCellMarch 5, 2020Ina Nemet et al.Scientific. cAMP Assay in MEG01, Platelets and HEK293 Cells Intracellular cAMP levels were measured using CatchPoint Cyclic-AMP Fluorescent Assay Kit (Molecular Devices). Briefly, MEG01 cells were washed with 1X HBSS buffer with HEPES (20 mM; pH 7.4)

(8) A multicenter, randomized, rater-blinded, parallel-group, phase 3 study to compare the efficacy, safety, and immunogenicity of biosimilar RGB-10 and reference once-daily teriparatide in patients with osteoporosisOsteoporosis InternationalOctober 1, 2019H. Hagino, R. Narita, Y. Yokoyama, M. Watanabe, M. TomomitsuRockville, MD, USA) and biotinylated RGB-10 and SULFO-TAG-labeled RGB-10. A validated cell-based assay (CatchPoint cyclic-AMP fluorescent assay kit; Molecular Devices Japan K.K., Tokyo, Japan) was used to measure neutralizing activities. Assessments

(9) Synthesis and pharmacological characterization of ethylenediamine synthetic opioids in human μ-opiate receptor 1 (OPRM1) expressing cellsPharmacology research & perspectivesOctober 1, 2019Hsu T, Mallareddy JR, Yoshida K, Bustamante V, Lee T, Krstenansky JL, Zambon AC.compounds, FSK, and NLX as a vehicle control. All solutions were removed and the cells were lysed with Catchpoint Cyclic‐AMP Fluorescent Assay Kit Lysis Buffer (Molecular Devices R8088) and shaken on a plate shaker for 15 minutes at room temperature.

(10) ASB20123: A novel C-type natriuretic peptide derivative for treatment of growth failure and dwarfismPloS oneJanuary 1, 2019Morozumi N, Yotsumoto T, Yamaki A, Yoshikiyo K, Yoshida S, Nakamura R, Jindo T, Furuya M, Maeda H, Minamitake Y, et al.concentration in each sample was determined by competitive enzyme-linked immunosorbent assay (ELISA) using the CatchPoint cGMP fluorescent assay kit (Molecular Devices Corporation, USA) and FlexStation (Molecular Devices Corporation,

(11) β-Adrenergic Receptor Trafficking, Degradation, and Cell Surface Expression Are Altered in Dermal Fibroblasts from Hypertrophic ScarsJournal of Investigative DermatologyJuly 1, 2018Amina El El Ayadi, Anesh Prasai, Ye Wang, David N. Herndon, Celeste C. Finnertylisted in Supplemental Table 1. cAMP and PKA activity assays Intracellular cAMP was measured using a Catchpoint cAMP assay kit (Molecular Devices, Sunnyvale, CA, USA) following the manufacturer’s protocol. The PKA

(12) Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitorNature communicationsMay 18, 2018Gulati S, Jin H, Masuho I, Orban T, Cai Y, Pardon E, Martemyanov KA, Kiser PD, Stewart PL, Ford CP, et al.cells were lysed and their intracellular cAMP levels were measured according to the procedures of the CatchPoint Cyclic-AMP Fluorescent Assay Kit (Molecular Devices, Sunnyvale, CA USA). Fluorescence with excitation/emission at 530/590 nm was read

(13) A novel small molecule chaperone of rod opsin and its potential therapy for retinal degenerationNature communicationsMay 17, 2018Chen Y, Chen Y, Jastrzebska B, Golczak M, Gulati S, Tang H, Seibel W, Li X, Jin H, Han Y, et al.cell culture incubator for 15 min at 37 °C in 5% CO2. Levels of accumulated cAMP were detected with the Catchpoint cAMP fluorescent assay kit (Molecular Devices, Sunnyvale, CA USA) and the fluorescence with excitation/emission

(14) Approaches for Probing Allosteric Interactions at 7 Transmembrane Spanning ReceptorsProgress in Molecular Biology and Translational ScienceApril 18, 2017Michael T. Klein, Paige N. Vinson, Colleen M. NiswenderHitHunter® cAMP assay system (DiscoveRx). Several other assay systems are commercially available (e.g., CatchPoint® cyclic-AMP, Molecular Devices; HTRF® cAMP kits, Cisbio; AlphaScreen® cAMP Assay, PerkinElmer; and various

(15) cAMP-dependent activation of protein kinase A attenuates respiratory syncytial virus-induced human airway epithelial barrier disruptionPloS oneJanuary 1, 2017Rezaee F, Harford TJ, Linfield DT, Altawallbeh G, Midura RJ, Ivanov AI, Piedimonte G.number. cAMP measurement Intracellular cAMP concentration in the clarified lysates was determined using CatchPoint cAMP kit (Molecular Devices; Sunnyvale, CA) as per manufacturer’s instructions. Statistical analysis Data

(16) Heterodimerization of the prostaglandin E2 receptor EP2 and the calcitonin receptor CTRPloS oneJanuary 1, 2017Matsubara S, Shiraishi A, Sakai T, Okuda T, Satake H.using a Ca5 kit (Molecular Devices, Sunnyvale, USA) and end point observation of cAMP production using CatchPoint Cyclic AMP Assay Kit (Molecular Devices) were performed on FlexStation II. Results are shown as means

(17) Design and Synthesis of Fluorescent Acyclic Nucleoside Phosphonates as Potent Inhibitors of Bacterial Adenylate CyclasesChemMedChemNovember 21, 2016Petra Břehová, Markéta Šmídková, Jan Skácel, Martin Dračínský, Helena Mertlíková-Kaiserová, Monica P. Soto Velasquez, Val J. Watts, Zlatko JanebaPalo Alto, CA; SA=115 µmol/min/mg) for 30 min. Finally, the cAMP content was determined by using the CatchPoint cAMP immunoassay kit (Molecular Devices, Wokingham, UK). After the addition of lysis buffer (50 µL per

(18) Receptor guanylyl cyclase‐ G is a novel thermosensory protein activated by cool temperaturesThe EMBO JournalFebruary 3, 2015Ying‐Chi Chao, Chih‐Cheng Chen, Yuh‐Charn Lin, Heinz Breer, Joerg Fleischer, Ruey‐Bing Yangwere exposed to 37 or 15°C for 20 min (with 1 mM IBMX), then cGMP content was measured by use of the CatchPoint cGMP fluorescent assay kit (Molecular Devices, Sunnyvale, CA, USA). cGMP concentration was determined

(19) High density and ligand affinity confer ultrasensitive signal detection by a guanylyl cyclase chemoreceptorThe Journal of Cell BiologyAugust 18, 2014Magdalena Pichlo et al.20,000 g and 4°C). The cGMP concentration in 40 µl of the supernatant was determined using an assay (CatchPoint Cyclic-AMP Fluorescent Assay Kit; Molecular Devices) according to the manufacturer’s instructions. To improve the sensitivity of the

(20) Amidate Prodrugs of 9-[2-(Phosphonomethoxy)Ethyl]Adenine as Inhibitors of Adenylate Cyclase Toxin from Bordetella pertussisAntimicrobial Agents and ChemotherapyFebruary 1, 2014Markéta Šmídková, Alexandra Dvořáková, Eva Tloušt'ová, Michal Česnek, Zlatko Janeba, Helena Mertlíková-Kaiserová0.4 ␮g of ACT/ml from B. pertussis for 30 min. Finally, the cAMP content was determined by using the CatchPoint cAMP immunoassay kit (Molecular Devices, Wokingham, United Kingdom). After the addition of lysis buffer

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CatchPoint® Cyclic-AMP Fluorescent Assay kit from Molecular Devices

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CatchPoint® Cyclic-AMP Fluorescent Assay kit from Molecular Devices

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