Description
Human Chromogranin A EIA Kit and and related products Back GroundChromogranin A (CgA) is an acidic secretory protein, consisting of 439 amino acid in human. The protein is found in a wide variety of hormone and neurotransmitter storage vesicles, and it is known to be co-stored and co-released with catecholamines from adrenal medulla and sympathetic neuronal vesicles during exocytosis. O’Conner and Bernstein have first reported radioimmunological measurement of CgA in human plasma under conditions of physiologic, pharmacologic and pathologic alteration of sympathoadrenal function. Nakane et al. recently discovered that CgA-LI exists in saliva, the concentration of which elevates rapidly under psychosomatic stress even prior to the elevation of salivary cortisol level. Subsequently, Kanno et al. presented an evidence for autonomic control of submandibular CgA-LI secretion in the anaesthetized rat.This assay kit succeeded in developing, for the first time, a specific, sensitive, stable and easy manipulative enzyme immunoassay (EIA) system for measurement of human CgA-LI using anti-synthetic human CgA(344-374) antibody, synthetic human CgA(344-374) as standard antigen and Na-biotinylglycylglycyl human CgA(344-374) as labeled antigen. The assay kit now available from the Institute can be used for measurement of CgA-LI in human biological fluid such as plasma, urine and saliva.