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Assay Kit
cAMP-Glo Assay
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cAMP-Glo Assay
cAMP-Glo Assay
cAMP-Glo Assay from Promega
(2)
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Citations:
(63)
Figures:
(1)
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cAMP-Glo Assay
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Description
The cAMP-Glo Assay is a homogeneous, bioluminescent and high-throughput assay for measuring cAMP levels in cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
cAMP-Glo Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V1501
Detection Target
cAMP-Glo
Quantity
300 assays
Method
Assay
Sample Type
cells
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (63)
(1)
Anti-secretory and anti-proliferative actions of next-generation dual subtype 2 and 5 somatostatin receptor ligands in neuroendocrine tumor models
Frontiers in Oncology
February 27, 2026
Francesco Fedeli, Margarita Bistika, Francesco Ascione, Alessandro Marangelo, Fabio L. Guzzi, Jörg Schrader, Alan G. Harris, Natalia S. Pellegata
Sigma-Aldrich
, #B8279). The
assay
was performed by using the
cAMP-Glo Assay
kit (
Promega Corporation
, #
V1501
) following the manufacturer’s instructions. Cell proliferation/viability
assay
For 2D cultures of
(2)
CREB suppresses PGRP-SC2 to drive age-related immune senescence and gut dysbiosis in Drosophila
Cell Death Discovery
February 24, 2026
Saifei Wang, Bohan Qi, Peng Ma, Yao Zhang, Youjie Yin, Shuxin Chen, Hansong Deng
tissue was determined by measuring the relative light units using a
cAMP-Glo™ Assay
kit (
Promega
, Cat#
V1501
), following the manufacturer’s instructions. In summary,
protease inhibitors
and the PDE
inhibitors
(3)
Targeting mechanosensitive cannabinoid receptor 1 with isoflavone prodrugs attenuates atherosclerotic endothelial dysfunction
Journal of Biomedical Science
January 21, 2026
Dai-Jung Chung, Shao-Peng Chen, Wei-Hsuan Liu, Chia-Yu Liu, Nan-Wei Su, Chen Hsu, Hsin-Ya Tsai, Kai-Chien Yang, Cho-Kai Wu, Sheng-Wei Lin,
et al.
measurement The cAMP concentration was measured via the
cAMP-Glo™ Assay
Kit (
Promega Corporation
,
V1501
, Madison, WI, USA).
Endothelial cells
(HUVECs, HAECs, and
hiPSC
-ECs) were seeded into 6-well plates
(4)
TRPS1 confers paclitaxel resistance in TNBC through dual activation of the PDE4D–cAMP–AKT pathway and microtubule destabilization
Breast Cancer Research
January 20, 2026
Xin He, Wanting Tong, Xinyan Chen, Huifen Huang, Na Wei, Jiayue Ma, Yuqiong Liu, Yihui Ma, Shenglei Li, Wencai Li,
et al.
control group
. cAMP assay Intracellular cAMP levels were quantified using the
cAMP-Glo™ Assay
Kit (
Promega
V1501
). Cells were seeded in 96-well plates (8 × 103 cells/well) and either
transfected
with plasmids via
(5)
Caffeine Mitigates Adenosine-Mediated Angiogenic Properties of Choroidal Endothelial Cells Through Antagonism of A1 Adenosine Receptor and PI3K-AKT Axis
Cells
January 5, 2026
SunYoung Park, Yong-Seok Song, Xuan Feng, Christine M. Sorenson, Nader Sheibani
cAMP Measurements The intracellular cAMP levels in ChEC were determined using the
cAMP-Glo Assay
(
V1501
;
Promega
), according to the manufacturer’s instructions. Briefly, cells (3 × 103) were plated in white
(6)
Cannabidiol does not cause DNA double-strand breaks in a human liver-derived cell model
Journal of Cannabis Research
December 12, 2025
Romano Weiss, Victoria Liedtke, Stefan Rödiger
NucDetect software with manual count adjustments. cAMP assay
Promega
’s
cAMP-Glo™ assay
(
cAMP-Glo™ Assay
(
V1501
),
Promega
, Wisconsin, USA) was used to monitor the response to the CBD treatment on the G protein-coupled
(7)
Mitochondrial complex III subunit Qcr8 regulates the virulence and adhesion of Candida albicans by modulating mitochondrial function
Microbiol Spectr
December 8, 2025
Qianjun Zhao, Xiaotian Huang, Qiong Liu, Yanli Cao, Xiaomin Yu, Yanling Liu, Niya Hu, Zhenning Han, Junjun Tan, Xuan Li,
et al.
(
BacTiter-Glo Microbial Cell Viability Assay
;
Promega
, TB337, USA) and the cAMP test kit (
cAMP-Glo Assay
;
Promega
,
V1501
). Statistical analysis All experiments were performed in triplicate. Data are presented as mean ±
(8)
Dual interference with host neuropeptide signaling allows parasitoid wasp to hijack host sugar metabolism
The EMBO Journal
November 26, 2025
Zhi-Zhi Wang, Ruo-Fei Ma, Li-Cheng Gu, Li-Zhi Wang, Ting Chen, Pei Yang, Jia-Ni Zou, Jiang-Yan Zhu, Zhi-Wei Wu, Yue-Nan Zhou,
et al.
SuperScript III First-Strand Synthesis System
,
Invitrogen
,
18080051
5 min TA/Blunt-Zero Cloning Kit,Vazyme,C601
cAMP-Glo™ Assay
,
Promega
,Cat#
V1501
Glucose (HK) Assay kit,Sigma-Aldrich,Cat# GAHK20-1KT Glucose (GO) Assay Kit,Sigma-Aldrich,Cat# GAGO20 Glucose-6-Phosphate
(9)
Virulence Plasmid Modulates Glucose-Mediated Biofilm Regulation in Yersinia enterocolitica
Life (Basel)
October 30, 2025
Yunah Oh, Tae-Jong Kim
glucose-mediated changes in intracellular cAMP levels were measured using the
cAMP-Glo™ Assay
Kit (Catalog number:
V1501
;
Promega
Korea Ltd., Seoul, Republic of Korea) following the manufacturer’s protocol. Bacterial cultures
(10)
The small GTPase Rho5—Yet another player in yeast glucose signaling
PLOS Genetics
September 9, 2025
Franziska Schweitzer, Linnet Bischof, Stefan Walter, Silke Morris, Hans-Peter Schmitz, Jürgen J. Heinisch
of a kit originally designed to
measure
cAMP concentration (
cAMP-Glo Assay
,
Promega
product number
V1501
). As a modification, only the Kemptid peptide substrate and the luciferase coupling was used from the
(11)
A small heat shock protein Fmp28 influences virulence by regulating Als3 expression via the cAMP-PKA signaling pathway in Candida albicans
mBio
August 13, 2025
Junjun Tan, Qiong Liu, Zhiping Liu, Yanli Cao, Xiaomin Yu, Qianjun Zhao, Niya Hu, Yanling Liu, Yuwei Wan, Yeming Zhang,
et al.
the concentration of cAMP, the samples were similarly prepared using a
cAMP-Glo Assay
Kit (
Promega
,
V1501
), following the manufacturer’s instructions before measuring by a luminometer.
Cell staining
by immunofluorescence
Cells
(12)
Extracellular Metallothionein: An Alarmin Regulating Lymphocyte Gene Expression, Cell Signaling, and Immune Function
Cell Stress and Chaperones
July 31, 2025
Michele Maltz-Matyschsyk, Clare K. Melchiorre, Kristen Dostie-Laprise, Michael A. Lynes
using the
cAMP-GLO™ assay
according to the manufacturer’s instructions (
Promega
; Madison, WI; Cat#
V1501
). Briefly, Jurkat
T cells
were suspended to 2×106 cells/ml in induction buffer, and 100 µl (20,000 cells/well)
(13)
Multiomic profiling reveals that prostaglandin E2 reverses aged muscle stem cell dysfunction, leading to increased regeneration and strength
Cell Stem Cell
July 3, 2025
Yu Xin Wang, Adelaida R. Palla, Andrew T.V. Ho, Daniel C. L. Robinson, Meenakshi Ravichandran, Glenn J. Markov, Thach Mai, Chris Still, Akshay Balsubramani, Surag Nair,
et al.
Click-iT EdU Alexa Fluor 555 Imaging kit
,
Thermo Fisher Scientific
,Catalog#
C10338
cAMP-Glo Assay
protocol,
Promega
,Catalog#
V1501
Nextera
DNA Library Prep
Kit,Illumina,Catalog# FC-121–1030 Deposited Data,, Raw and analyzed RNASeq
(14)
Using sodium glycodeoxycholate to develop a temporary infant-like gut barrier model, in vitro
Front Nutr
June 9, 2025
Francesca Bietto, Elena Arranz, Beatriz Miralles, Cristina Gómez-Marín, Eva Rath, Alice J. Lucey, Linda Giblin
70.3050.255).
CellTiter 96™ AQueous One Solution Cell Proliferation Assay
(MTS,
G3582
) and
cAMP-Glo™ Assay
kit (
V1501
) were purchased from
Promega
(USA). CyQUANT™ LDH Cytotoxicity Assay (C20300) was purchased from Invitrogen
(15)
IGF1R/ARRB1 Mediated Regulation of ERK and cAMP Pathways in Response to Aβ Unfolds Novel Therapeutic Avenue in Alzheimer's Disease
Molecular neurobiology
June 1, 2025
Sengupta P, Mukhopadhyay D.
Assay
The cyclic AMP
assay
was performed according to the manufacturer’s protocol (
Promega
Cat. No.
V1501
). In brief, cells were seeded equally on poly-L-lysine-coated 96-well plates after counting on a hemocytometer,
(16)
Arctiin, a lignan compound, enhances adipose tissue browning and energy expenditure by activating the adenosine A(2A) receptor
Molecular medicine (Cambridge, Mass.)
May 14, 2025
Gu Y, He W, Li W, Cai J, Wang Z, Li K, Qin G, Gu X, Lin X, Ma L,
et al
.
T2939
) and
ZM241385
(
MCE
,
HY-19532
) for 30 min and subsequently assayed following the cAMP kit (
Promega
,
V1501
) protocol.
Western blot
Adipose tissue
or cells were extracted using RIPA buffer (Beyotime, China)
(17)
Bioengineering the metabolic network of CAR T cells with GLP-1 and Urolithin A increases persistence and long-term anti-tumor activity
Cell Reports Medicine
March 18, 2025
Areej Akhtar, Md Shakir, Mohammad Sufyan Ansari, Divya, Md Imam Faizan, Varnit Chauhan, Aashi Singh, Ruquaiya Alam, Iqbal Azmi, Sheetal Sharma,
et al.
assay
,
Thermo Fisher Scientific
,
M34153
Human GLP1 (7–36) ELISA Kit
,
Abcam
,
ab184857
cAMP-Glo™ Assay
,
Promega
,
V1501
,, Deposited data,Deposited data,Deposited data ,,
scRNA-seq
data for patient CAR
T cells
,Good et
(18)
Functional characterization of OR51B5 and OR1G1 in human lung epithelial cells as potential drug targets for non-type 2 lung diseases
Cell biology and toxicology
November 13, 2024
Awad N, Weidinger D, Greune L, Kronsbein J, Heinen N, Westhoven S, Pfaender S, Taube C, Reuter S, Peters M,
et al
.
intracellular cAMP concentration was determined using cAMP-GloTM
Assay
(
Promega
, Wisconsin, USA, cat:
V1501
) according to the manufacturer’s guidelines. Phospholipase C (PLC)
assay
3 × 106
A549 cells
were cultured
(19)
Novel immunomodulatory properties of adenosine analogs promote their antiviral activity against SARS-CoV-2
EMBO Reports
July 15, 2024
Giulia Monticone, Zhi Huang, Peter Hewins, Thomasina Cook, Oygul Mirzalieva, Brionna King, Kristina Larter, Taylor Miller-Ensminger, Maria D Sanchez-Pino, Timothy P Foster,
et al.
Cho cell
line) using a cAMP competitive ELISA kit (Abcam) or a
luciferase reporter assay
(cAMP-Glow
Assay
,
V1501
,
Promega
) according to the manufacturer’s protocol.
Western blotting
Protein lysates were loaded on
(20)
GDF15 controls primary cilia morphology and function thereby affecting progenitor proliferation
Life Science Alliance
July 1, 2024
Katja Baur, Şeydanur Şan, Gabriele Hölzl-Wenig, Claudia Mandl, Andrea Hellwig, Francesca Ciccolini
incubated for 30 min at 37°C, 5% CO2. Afterwards, cAMP levels were measured using a
cAMP-Glo Assay
(#
V1501
;
Promega
) according to the manufacturer’s instructions (protocol for adherent cells, technical bulletin
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Reviews
Write a Review
(1)
11/7/2022
Great cAMP Measurement Kit for GPCR Screen
Bruce Wang
We are interested in the potential function of an endogenous metabolite in human serum. We use this cAMP-Glo assay kit combined with our GPCR library cell lines to screen for potential GPCR receptors ...
Read Review
(1)
10/10/2016
cAMP Glo Assay Kit from Promega
Ganesh Pusapati
cAMP is an important second messenger in the cell that activates Protein Kinase A. cAMP is generated by the enzymes called adenylate cyclases that are activated by the GalphaS protein. In the present ...
Read Review
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cAMP-Glo Assay from Promega
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