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GSH/GSSG-Glo Assay
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GSH/GSSG-Glo Assay
GSH/GSSG-Glo Assay
GSH/GSSG-Glo Assay from Promega
(1)
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Citations:
(185)
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Promega for
GSH/GSSG-Glo Assay
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Description
The GSH/GSSG-Glo Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
GSH/GSSG-Glo Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V6611
Quantity
10ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (185)
(1)
Licochalcone A Inhibits Melanoma Cell Survival by Suppressing HSP75 (TRAP1) and Disrupting Cellular Redox and Energy Homeostasis
Int J Mol Sci
October 14, 2026
Yoon-Jin Lee, Min Hyuk Choi, Ki Dam Kim, Sukh Que Park, Youin Bae, Yu Sung Choi, Sang Han Lee, Hae-Seon Nam, Moon Kyun Cho
Glutathione Redox Status Intracellular glutathione redox status was assessed using the
GSH/GSSG-Glo™ Assay
(
V6611
;
Promega
, Madison, WI, USA) according to the manufacturer’s instructions. G361 and HEMn-MP cells were
(2)
Regulation of PDH via cysteine oxidation on DLAT
Nat Commun
October 14, 2026
Fabiola Garcia-Cortizo, Tobias Weber, Hana Nůsková, Daniel Pfaff, Michael Büttner, Angela Wirth, Elisabeth Kliemank, Gernot Poschet, Marc Freichel, Thomas Fleming,
et al.
redox status, GSH/GSSG and NADPH/NADP+ ratios were measured using the
GSH/GSSG-Glo™ Assay
Kit (
Promega
,
V6611
) and the
NADP/NADPH-Glo™ Assay
Kit (
Promega
,
G9082
), respectively, accordingly to the manufacturer’s
(3)
A defined isothiocyanate mixture suppresses platelet signaling and metabolism to limit thrombosis in vivo
Frontiers in Immunology
September 30, 2026
Jan Yağız Buchholz, Maria Shoykhet, Anthea C. Darius, Tobias Weiser, Erika Lynn Roberts, Mohammad Khosravi, Julie Petry, Seyedeh Zahra Mousavi, Babak Mohammadian, Lina Adams,
et al.
controls. Total and oxidized glutathione (GSH, GSSG) were quantified using the
GSH/GSSG-Glo assay
(#
V6611
,
Promega
) and expressed relative to unstimulated controls. Protein S-glutathionylation (PSSG) was measured
(4)
Adipocytes Protect Ovarian Cancer Cells from Ferroptosis
Biomolecules
September 30, 2026
Lia Tesfay, Chaeyeon Yoo, Timothy E. Moore, Jingyun Lee, Kirtikar Shukla, Cristina M. Furdui, Poornima Hegde, Evan R. Jellison, Suzy V. Torti
ACM, lysed, and the GSH/GSSG ratio measured using the GSH/GSSG Glo (
Promega
, Madison, WI, USA; cat#
V6611
) kit according to the manufacturer’s protocol. 2.7. Spheroid Formation and Viability Assessment A
(5)
Combined Arctigenin and Curcumin Treatment Induces Redox and Metabolic Stress, AMPK Phosphorylation, and Apoptotic Signaling in Prostate Cancer Cells
Nutrients
September 10, 2026
Moon-Kyun Cho, Sang-Han Lee, Hae-Seon Nam, Dongsic Choi, Yoon-Jin Lee
of reduced glutathione (GSH) to oxidized glutathione (GSSG) using the
GSH/GSSG-Glo™ Assay
(Cat. No.
V6611
;
Promega
, Madison, WI, USA). Following 48 h of
incubation
, HPrEC and
PC-3 cells
were processed according
(6)
A lysis-derived PMSF and orthovanadate oxidant defines a distinct cysteine-engagement profile
Cell Chemical Biology
August 31, 2026
Bethany E. Schaffer, Jung Seung Nam, Ceci A. Cohen, Clementina Mesaros, Robert W. Myers, Leigh Baxt, Kathryn E. Wellen, Lewis C. Cantley, Noah Dephoure, Nathaniel W. Snyder,
et al.
confluence. Total or oxidized glutathione was then measured using the
GSH/GSSG-Glo Assay
(
Promega
,
V6611
) per the manufacturer’s instructions. The average
luminescence
from duplicate wells was calculated and
(7)
COPZ1 depletion induces cell death through different mechanisms in in vitro murine models of thyroid cancer
Eur Thyroid J
August 17, 2026
Tiziana Di Marco, Debora Vergaro, Mara Mazzoni, Beatrice Mazzoleni, Eleonora Muraro, Sonia Pagliardini, Angela Greco
oxidized glutathione was accomplished using the
luminescence
-based system of GSH/GSSG-GloTM
Assay
(#
V6611
) by
Promega Corporation
(USA) according to the manufacturer’s instructions.
Luminescence
was measured
(8)
Identification of a NUAK1/2 Inhibitor as a Macrophage-Polarizing Compound by a Machine Learning-Based Phenotypic Cell Painting Screen
Int J Mol Sci
August 14, 2026
Johanna B. Brüggenthies-Brunner, Jakob Dittmer, Julia Sauer, Sarah Groetzner, Anika Liu, Eva Martin, Anja Teufel, Christine Strasser, Helga Bronner, Robert Ries,
et al.
corresponding figure legends. To quantify total glutathione ratios, a luminescent -based
GSH/GSSG-Glo™ Assay
(
V6611
,
Promega
, Walldorf, Germany) was performed following the manufacturer’s instructions for adherent cells
(9)
Polyunsaturated fatty acid sequestration protects against mitochondrial dysfunction-induced ferroptosis
EMBO Reports
August 14, 2026
Guillermo Puertas-Frías, María José Saucedo-Rodríguez, Kristýna Čunátová, Lukáš Alán, Patrik Hrbáč, Michal Knězů, Soňa Koníčková, Jakub Schimmer, Monika Krakovková, Marek Vrbacký,
et al.
buffer,
Thermo Fisher Scientific
,
37570
NAD + /NADH Glo assay
,
Promega
,
G9071
GSH/GSSG Glo assay
,
Promega
,
V6611
Software,Software,Software GraphPad Prism 10,GraphPad software,
Gen5 software
,
BioTek
, FlowJo 10,BD
(10)
Combinatorial Effects of Plasma-Treated Solution and Plasma-Treated Hydrogel with Cisplatin in Vulvar Cancer Cells
Oncology Research
August 13, 2026
Estelle C. I. D. Schad, Janet P. Raja Xavier, Hortense Decool, Marcel Arnholdt, Franziska Keßler, Jan Schöttke, Sara Y. Brucker, Ernst Oberlechner, Johanna Laupp, Martin Weiss
(GSH/GSSG) Intracellular GSH and GSSG levels were measured using the
GSH/GSSG-Glo™ assay
(
Promega
, #
V6611
), following the supplier’s protocol. SW954 cells were subjected to the previously introduced combination
(11)
Myocilin drives cardiomyocyte mitochondrial dysfunction via SLC3A2-dependent redox imbalance in heart failure
Redox Biol
August 12, 2026
Xiaoyue Yu, Junxia Zhang, Junfang Wu, Mingming Zhao, Hongtu Cui, Sen Huang, Jiawen Liu, Minwen Long, Yandong Pei, Aiming Liu,
et al.
of total glutathione and oxidized glutathione (GSSG) were quantified using the
GSH/GSSG-Glo™ Assay
(
V6611
,
Promega
, USA). Briefly, AC16 cells were lysed with either Total or Oxidized Glutathione Lysis Reagent;
(12)
Early-life oxidative stress programs persistent NFκB activation and neuroinflammation in autism spectrum disorder
Front Immunol
August 10, 2026
Yun Jiao, Qingzheng Jia, Xiaozhuang Zhang, Claire Zhang, Chongxia Wei, Dakota Wang, Xiaohan Liu, Huilin Li, Alex Hu, Liqin Zeng,
et al.
ratio, indicating glutathione redox status, was determined by the
GSH/GSSG-Glo™ Assay
Kit (
Promega
,
V6611
) as per supplier’s protocol. Malondialdehyde quantification MDA levels in plasma and tissues were
(13)
Doxorubicin‐Induced Cytotoxicity in Adipose‐Derived Stem Cells Is Associated With Altered SAPK/JNK Signaling
FASEB J
July 30, 2026
Aleksandra Skubis‐Sikora, Kinga Pogoda‐Mieszczak, Bartosz Sikora, Edyta Bogunia, Aleksandra Bryzek, Piotr Czekaj
performed as six biological replicates (N = 6). 2.9.2
GSH/GSSG‐Glo Assay
The
GSH/GSSG‐Glo assay
(
V6611
;
Promega
, Madison, WI, USA) was used to
measure
the levels of reduced glutathione (GSH), oxidized glutathione
(14)
Metabolic targeting of cyst(e)ine enhances carboplatin responsiveness in ovarian cancer
Cancer & Metabolism
July 29, 2026
Ina Yoon, Sinae Oh, Seo Young Park, Jisu Park, Hyelim Jang, Eunji Park, Kyung Ah You, Kibum Kim, Soonjae Hwang, Hyeong Yun Kim,
et al.
glutathione (GSSG) and total glutathione (GSSG + GSH) were measured using the
GSH/GSSG-Glo™ Assay
(
Promega
,
V6611
) according to the manufacturer’s instructions. Briefly, cells were lysed using either Total or Oxidized
(15)
Targeting chordoma via an isocitrate dehydrogenase-1-dependent susceptibility to redox metabolism
Acta Neuropathol
July 23, 2026
Matthew Pun, Akash Deogharkar, Siva Kumar Natarajan, Nicholas Nuechterlein, Wentao Tian, Sunjong Ji, Eleanor Young, Fusheng Yang, Debra Hawes, Anthony C. Andren,
et al.
inhibitor
at 3 μM for 24 or 48 h. Subsequently, cells were processed with the
GSH/GSSG-Glo Assay
(
Promega
,
V6611
) which quantifies total and oxidized glutathione. Ratios were calculated from luminescent intensity
(16)
A targetable FTO/SLC7A11/CBS/CTH axis controls cysteine metabolism, growth and survival in NSCLC
Sci Adv
July 3, 2026
Nishanth Kuganesan, Margaret Pan, Haowen Jiang, Stavros Melemenidis, Jiangbin Ye, Sara Richter, Maximillian Diehn, Kerriann M. Casey, Edward E. Graves, Quynh Thu Le,
et al.
following day, the GSH/GSSG ratio and total GSH were determined using GSH/GSSG-Glo (
Promega
, catalog no.
V6611
) according to the manufacturer’s instructions. For pharmacological inhibition of
FTO
, H1299 (10,000
(17)
Metabolic plasticity and optimal redox homeostasis are essential for efficient metastatic colonization
Molecular Metabolism
July 1, 2026
Ece Grace, Deyu Zou, Romy Böttcher-Loschinski, Martin Böttcher, Harald Schuhwerk, Yussuf Hajjaj, Annemarie Schwab, Simon Brandt, Ana Clavel Ezquerra, Witold Szymanski,
et al.
oxidized glutathione (GSH and GSSG) levels were determined using the
GSH/GSSG-Glo™ Assay
(
Promega
,
V6611
), following the manufacturer’s instructions. Standard curves were generated using purified NAD (Sigma–Aldrich,
(18)
Ferrostatin-1 and hinokitiol supplementation enhance human hematopoietic stem cell expansion in a chemically defined medium
Mol Ther Adv
June 11, 2026
Li L, Mandal PK.
CellTiter-Glo Luminescent Cell Viability Assay
kit (Catalog #
G7571
) and
GSH/GSSG-Glo assay
reagent (Catalog #
V6611
) were purchased from
Promega
(Madison, WI). Iscove’s modified Dulbecco’s medium (IMDM, Catalog # 12440053),
(19)
Nickel exposure disrupts epigenetic repression of developmental genes in mouse embryonic stem cells
Toxicology and Applied Pharmacology
June 1, 2026
Beatrix Bradford, David Filipovic, Xiaoru Zhang, Viktoriia Goriainova, Suresh Cuddapah
ratio of reduced (GSH) to oxidized glutathione (GSSG) was determined using the
GSH/GSSG-Glo™ Assay
Kit (
V6611
,
Promega
) according to the manufacturer’s protocol. Statistical significance was assessed using a t-test. 2.4.
(20)
Non-invasive radiogenomic mapping of the SMARCAL1-driven ferroptotic niche is associated with longitudinal MRD-negative surveillance in early-stage NSCLC
Frontiers in Immunology
May 25, 2026
Zehao Huang, JunDao He, Yue Li, ZhanYu Xu, Huajian Peng, Xiang Gao, HuaFu Zhou, JianJi Guo, Nuo Yang
Cayman Chemical
10009055
, normalized to protein), reduced glutathione (GSH/GSSG-Glo
Assay
,
Promega
V6611
, normalized to
cell count
), and
transmission electron microscopy
for shrunken
mitochondria
with increased
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Reviews
Write a Review
(1)
12/16/2021
Good for GSH/GSSG Ratio Detection Assay Kit
Kumar Subramani
Reliable product.
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GSH/GSSG-Glo Assay from Promega
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