GSH/GSSG-Glo Assay V6611 from Promega

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ItemGSH/GSSG-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV6611
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Metabolic plasticity and optimal redox homeostasis are essential for efficient metastatic colonizationMolecular MetabolismJuly 1, 2026Ece Grace, Deyu Zou, Romy Böttcher-Loschinski, Martin Böttcher, Harald Schuhwerk, Yussuf Hajjaj, Annemarie Schwab, Simon Brandt, Ana Clavel Ezquerra, Witold Szymanski, et al.oxidized glutathione (GSH and GSSG) levels were determined using the GSH/GSSG-Glo™ Assay (Promega, V6611), following the manufacturer’s instructions. Standard curves were generated using purified NAD (Sigma–Aldrich,

(2) Ferrostatin-1 and hinokitiol supplementation enhance human hematopoietic stem cell expansion in a chemically defined mediumMolecular Therapy AdvancesJune 11, 2026Lushen Li, Pankaj K. MandalCellTiter-Glo Luminescent Cell Viability Assay kit (Catalog #G7571) and GSH/GSSG-Glo assay reagent (Catalog #V6611) were purchased from Promega (Madison, WI). Iscove’s modified Dulbecco’s medium (IMDM, Catalog # 12440053),

(3) Nickel exposure disrupts epigenetic repression of developmental genes in mouse embryonic stem cellsToxicology and Applied PharmacologyJune 1, 2026Beatrix Bradford, David Filipovic, Xiaoru Zhang, Viktoriia Goriainova, Suresh Cuddapahratio of reduced (GSH) to oxidized glutathione (GSSG) was determined using the GSH/GSSG-Glo™ Assay Kit (V6611, Promega) according to the manufacturer’s protocol. Statistical significance was assessed using a t-test. 2.4.

(4) Non-invasive radiogenomic mapping of the SMARCAL1-driven ferroptotic niche is associated with longitudinal MRD-negative surveillance in early-stage NSCLCFrontiers in ImmunologyMay 25, 2026Zehao Huang, JunDao He, Yue Li, ZhanYu Xu, Huajian Peng, Xiang Gao, HuaFu Zhou, JianJi Guo, Nuo YangCayman Chemical 10009055, normalized to protein), reduced glutathione (GSH/GSSG-Glo Assay, Promega V6611, normalized to cell count), and transmission electron microscopy for shrunken mitochondria with increased

(5) Inflammatory CD4+ T cells can waive NRF2-dependent SLC7A11-mediated cystine uptake by using ASCT1iScienceMay 15, 2026Christopher Thomas Neullens, Sudheendra Hebbar Subramanyam, Gerd Horneff, Tilmann Kallinich, Freya Huijsmans, Jorg van Loosdregt, Bas Vastert, Klaus Tenbrock, Kim Ohlmeasurement and the calculation of GSH/GSSG ratio was conducted according to the manufacturer’s instructions (V6611, Promega). Quantification and statistical analysis Flow cytometric analysis and gating was performed

(6) Pseudogene-Derived Long Noncoding RNAs GSTM3P1/Gstm2-ps1 Exacerbate Sepsis-Associated Acute Kidney Injury by Suppressing Their Parent Gene TranslationThe American Journal of PathologyMay 1, 2026Jing Huang, Zheng Dong, Qingqing Weiglutathione ratio was measured using a luminescence-based glutathione assay kit (Promega, Madison, WI; V6611) following the manufacturer’s instruction. The intracellular reactive oxygen species (ROS) levels were

(7) Senescent Stroma-Derived Glutamine: A Driver of Aggressiveness in Prostate and Ovarian Cancer CellsCellsApril 24, 2026Giulia Lori, Caterina Mancini, Caterina Paffetti, Dayana Desideri, Erica Pranzini, Alice Santi, Manuela Leri, Alessio Biagioni, Matteo Benelli, Pietro Spatafora, et al.(GSSG) glutathione levels were measured using the GSH/GSSG-Glo™ Assay (Promega, Fitchburg, WI, USA V6611), according to the manufacturer’s instructions. Briefly, cells were lysed and total glutathione (GSH

(8) Stress adaptation pathways and HA–CD44 signaling maintain the survival of pancreatic cancer cells with centrosome amplificationCell Communication and SignalingApril 7, 2026Selahattin Can Ozcan, Evrim Goksel, Batuhan Mert Kalkan, Enes Cicek, Beste Kanevetci, Ceyda Acilanassays Intracellular reduced and oxidized glutathione levels were quantified using the GSH/GSSG-Glo assay (Promega, V6611) according to the manufacturer’s instructions. Control and centrosome-amplified (5d) cells were seeded

(9) ZNF395 is a Hypoxia-Responsive Regulator of Mitochondrial Glutaminolysis in Clear Cell Renal Cell CarcinomaCancer ResApril 2, 2026Joanna Koh, Chengheng Liao, Michelle Shu Wen Ng, Jing Han Hong, Hong Lee Heng, Dan Y. Gui, Zhenxun Wang, Benjamin Yan-Jiang Chua, Zhimei Li, Radoslaw M. Sobota, et al.without doxycycline (0.10 μg/mL), on the day prior to carrying out the GSH/GSSG-Glo™ assay (Promega V6611). Cells were lysed with either Total Glutathione Lysis Reagent to measure total GSH level or Oxidised

(10) Methionine metabolism is linked with phospholipid and glutamine metabolism to drive ferroptosisCell ReportsApril 1, 2026Jong Woo Kim, Seo Young Jang, Yeon Jin Roh, Yeajin Ju, Ju-Bin Kang, Byung-Sun Park, Ji-Yoon Lee, Min Wook Kim, Byung Jun Jeong, Woosung Jung, et al.cell culture plates (Corning, 3596). Glutathione levels were measured using a GSH/GSSG-Glo Assay Kit (Promega, V6611), and the experiments were carried out in accordance with the manufacturer’s protocol. To ensure that

(11) Monophosphoryl lipid A boosts macrophage antimicrobial immunity by metabolically regulating source-specific ROS generationFrontiers in ImmunologyMarch 30, 2026Dan Hao, Benjamin D. Klein, Margaret A. McBride, Julia K. Bohannon, Naeem K. Patil, Xenia D. Davis, Mary A. Oliver, Mei Lin Ning Dye, Sara Weidenbach, Jamey D. Young, et al.reader. GSH/GSSG quantification GSH and GSSG levels were measured using the GSH/GSSG-Glo™ Assay (Promega, V6611) according to the manufacturer’s protocol. ATP quantification Cells (5 ×105) were lysed with Luciferase

(12) Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosisImmunityFebruary 24, 2026Ana C. Codo, Jesus E. Romero-Pichardo, Zhaoquan Wang, Mariano A. Aufiero, Tomi Lazarov, Waleska Saitz Rojas, Nicole S. Walker, Achuth Nair, Roger F. Cole, Savannah Adkins, et al.Kit,Invitrogen,Cat#EEA003 Alanine Aminotransferase (ALT/GPT) Activity Kit,Invitrogen,Cat#EEA001 GSH/GSSG-Glo Assay,Promega,Cat#V6611 NADP/NADPH-Glo Assay,Promega,Cat#G9081 Seahorse XF Glycolytic Rate Assay Kit,Agilent Technologies,Cat#103344-100 Seahorse

(13) Microplastic-Induced Macrophage Dysfunction Drives Lung Tumor Progression through Glutathione ImbalanceACS NanoFebruary 24, 2026Bora Kim, Koung-Min Park, Haerang Lee, Young-Min Hyunpolystyrene MPs. GSH and GSSG levels were quantified using a luminescence-based GSH/GSSG-Glo assay (Promega, V6611) according to the manufacturer’s instructions. Luminescence-based measurements of the GSH/GSSG ratio

(14) Enhancing 5-fluorouracil efficacy in colorectal cancer by inhibiting glutathione antioxidant mechanisms with an xCT inhibitorScientific ReportsFebruary 22, 2026Senanur Malcanlı, Remzi Okan Akar, Engin Ulukaya, Ali Burak Özkaya, Öykü Gönül Geyiklevels, the amount of GSH/GSSG was determined in HCT-116 cells. “GSH/GSSG Glo Assay (Promega CAT NO: V6611)” was used in accordance with the manufacturer’s instructions to determine the GSH/GSSG levels in CRC

(15) Ferroptosis induces heterogeneous death profiles that are controlled by lysosome ruptureDevelopmental CellFebruary 17, 2026Jyotirekha Das, Saloni K. Hombalkar, Alison D. Klein, Esraa Nsasra, Muskaan Vasandani, Kay Petruzzi, Dajun Lu, Stephen Ruiz, Orit Kliper-Gross, Jiachen Hu, et al.(S11368; Molecular Probes) was used at a concentration of 15nM for all experiments. GSH/GSSG-Glo™ Assay (V6611; Promega) was used as per manufacturer’s guidelines. The GFP-Galectin-3, TFEB-GFP, and H2B-mCherry constructs

(16) GPX4 Inhibitor Resistance and Metastatic Features in Triple‐Negative Breast CancerAdvanced ScienceFebruary 17, 2026Marie Sabatier, Mayher Kaur, Milena Chaufan, Felix‐Levin Hormann, Luiza Martins Nascentes Melo, Mario Palma, Jordan Torpey, Yanshan Liang, Alanis Carmona, Cameron Fraser, et al.Measurements NADP+/NADPH and GSH/GSSG ratios were assessed using NADP/NADPH‐Glo (Cat#G9081) and GSH/GSSG‐Glo (Cat#V6611) assays purchased from Promega. These bioluminescence‐based assays rely on the proportional relationship

(17) A genetically encoded bifunctional enzyme mitigates redox imbalance and lipotoxicityNature MetabolismFebruary 16, 2026Xingxiu Pan, Subrata Munan, Austin L. Zuckerman, Andrew Pon, Niklas B. Thompson, Rasangi M. Perera, Nirajan Shrestha, Sara Violante, Justin R. Cross, Russell P. Goodman, et al.Glutathione Lysis Reagent or Oxidized Glutathione Lysis Reagent from the GSH/GSSG-Glo Assay kit (Promega, V6611) and samples were processed as previously described7. Live cell imaging using biosensors SoNar and

(18) Semaglutide Protects Retinal Ganglion Cells Against Rotenone-Induced Degeneration via Improved Glucose MetabolismInvest Ophthalmol Vis SciFebruary 10, 2026Zaynab A. Mouhammad, James R. Tribble, Alan Nicol, Evgenia Andreopoulou, Mariana Y. García-Bermúdez, Blanca I. Aldana, Rupali Vohra, Miriam Kolko, Pete A. WilliamsGSH/GSSG ratio were quantified using the GSH/GSSG-Glo Assay (Promega Corporation, Madison, WI, USA; Cat. V6611/V6612). The assay was performed according to the manufacturer's instructions. For each sample, 25 µL

(19) ZNF395 Is a Hypoxia-Responsive Regulator of Mitochondrial Glutaminolysis in Clear Cell Renal Cell CarcinomaCancer ResFebruary 4, 2026Joanna Koh, Chengheng Liao, Michelle Shu Wen Ng, Jing Han Hong, Hong Lee Heng, Dan Y. Gui, Zhenxun Wang, Benjamin Yan-Jiang Chua, Zhimei Li, Radoslaw M. Sobota, et al.without doxycycline (0.10 μg/mL), on the day prior to carrying out the GSH/GSSG-Glo™ assay (Promega V6611). Cells were lysed with either Total Glutathione Lysis Reagent to measure total GSH level or Oxidised

(20) The origin of hepatocellular carcinoma depends on metabolic zonationScienceJanuary 29, 2026Jason Guo, Roger Liang, Andrew Chung, Zhijie Li, Boyuan Li, Eric Chen, Lin Li, Jingjing Wang, Meng-Hsiung Hsieh, Ivy Xiangyi Fang, et al.liproxstatin-1. After 6 hours, GSH/GSSG was assessed using the GSH/GSSG-Glo assay kit (Promega Corporation #V6611) according to the manufacturer’s instructions. Statistics GraphPad Prism (version 10.0.2) software

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12/16/2021Good for GSH/GSSG Ratio Detection Assay Kit Kumar SubramaniReliable product.

GSH/GSSG-Glo Assay

GSH/GSSG-Glo Assay from Promega

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GSH/GSSG-Glo Assay from Promega

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