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Assay Kit
GSH/GSSG-Glo Assay
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GSH/GSSG-Glo Assay
GSH/GSSG-Glo Assay
GSH/GSSG-Glo Assay from Promega
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Citations:
(171)
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GSH/GSSG-Glo Assay
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Description
The GSH/GSSG-Glo Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
GSH/GSSG-Glo Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V6611
Quantity
10ml
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (171)
(1)
Doxorubicin‐Induced Cytotoxicity in Adipose‐Derived Stem Cells Is Associated With Altered SAPK/JNK Signaling
FASEB J
July 30, 2026
Aleksandra Skubis‐Sikora, Kinga Pogoda‐Mieszczak, Bartosz Sikora, Edyta Bogunia, Aleksandra Bryzek, Piotr Czekaj
performed as six biological replicates (N = 6). 2.9.2
GSH/GSSG‐Glo Assay
The
GSH/GSSG‐Glo assay
(
V6611
;
Promega
, Madison, WI, USA) was used to
measure
the levels of reduced glutathione (GSH), oxidized glutathione
(2)
Targeting chordoma via an isocitrate dehydrogenase-1-dependent susceptibility to redox metabolism
Acta Neuropathol
July 23, 2026
Matthew Pun, Akash Deogharkar, Siva Kumar Natarajan, Nicholas Nuechterlein, Wentao Tian, Sunjong Ji, Eleanor Young, Fusheng Yang, Debra Hawes, Anthony C. Andren,
et al.
inhibitor
at 3 μM for 24 or 48 h. Subsequently, cells were processed with the
GSH/GSSG-Glo Assay
(
Promega
,
V6611
) which quantifies total and oxidized glutathione. Ratios were calculated from luminescent intensity
(3)
A targetable FTO/SLC7A11/CBS/CTH axis controls cysteine metabolism, growth and survival in NSCLC
Sci Adv
July 3, 2026
Nishanth Kuganesan, Margaret Pan, Haowen Jiang, Stavros Melemenidis, Jiangbin Ye, Sara Richter, Maximillian Diehn, Kerriann M. Casey, Edward E. Graves, Quynh Thu Le,
et al.
following day, the GSH/GSSG ratio and total GSH were determined using GSH/GSSG-Glo (
Promega
, catalog no.
V6611
) according to the manufacturer’s instructions. For pharmacological inhibition of
FTO
, H1299 (10,000
(4)
Metabolic plasticity and optimal redox homeostasis are essential for efficient metastatic colonization
Molecular Metabolism
July 1, 2026
Ece Grace, Deyu Zou, Romy Böttcher-Loschinski, Martin Böttcher, Harald Schuhwerk, Yussuf Hajjaj, Annemarie Schwab, Simon Brandt, Ana Clavel Ezquerra, Witold Szymanski,
et al.
oxidized glutathione (GSH and GSSG) levels were determined using the
GSH/GSSG-Glo™ Assay
(
Promega
,
V6611
), following the manufacturer’s instructions. Standard curves were generated using purified NAD (Sigma–Aldrich,
(5)
Ferrostatin-1 and hinokitiol supplementation enhance human hematopoietic stem cell expansion in a chemically defined medium
Molecular Therapy Advances
June 11, 2026
Lushen Li, Pankaj K. Mandal
CellTiter-Glo Luminescent Cell Viability Assay
kit (Catalog #
G7571
) and
GSH/GSSG-Glo assay
reagent (Catalog #
V6611
) were purchased from
Promega
(Madison, WI). Iscove’s modified Dulbecco’s medium (IMDM, Catalog # 12440053),
(6)
Nickel exposure disrupts epigenetic repression of developmental genes in mouse embryonic stem cells
Toxicology and Applied Pharmacology
June 1, 2026
Beatrix Bradford, David Filipovic, Xiaoru Zhang, Viktoriia Goriainova, Suresh Cuddapah
ratio of reduced (GSH) to oxidized glutathione (GSSG) was determined using the
GSH/GSSG-Glo™ Assay
Kit (
V6611
,
Promega
) according to the manufacturer’s protocol. Statistical significance was assessed using a t-test. 2.4.
(7)
Non-invasive radiogenomic mapping of the SMARCAL1-driven ferroptotic niche is associated with longitudinal MRD-negative surveillance in early-stage NSCLC
Frontiers in Immunology
May 25, 2026
Zehao Huang, JunDao He, Yue Li, ZhanYu Xu, Huajian Peng, Xiang Gao, HuaFu Zhou, JianJi Guo, Nuo Yang
Cayman Chemical
10009055
, normalized to protein), reduced glutathione (GSH/GSSG-Glo
Assay
,
Promega
V6611
, normalized to
cell count
), and
transmission electron microscopy
for shrunken
mitochondria
with increased
(8)
Inflammatory CD4+ T cells can waive NRF2-dependent SLC7A11-mediated cystine uptake by using ASCT1
iScience
May 15, 2026
Christopher Thomas Neullens, Sudheendra Hebbar Subramanyam, Gerd Horneff, Tilmann Kallinich, Freya Huijsmans, Jorg van Loosdregt, Bas Vastert, Klaus Tenbrock, Kim Ohl
measurement and the calculation of GSH/GSSG ratio was conducted according to the manufacturer’s instructions (
V6611
,
Promega
). Quantification and statistical analysis
Flow cytometric analysis
and gating was performed
(9)
PIEZO1 is Required for Acute Myeloid Leukemia Progression and Leukemia Stem Cell Maintenance via HIF1A‐SLC7A11 Axis‐Mediated Ferroptosis Defense
Advanced Science
May 10, 2026
Tiantian Zhang, Ziyan Cao, Kexin Gao, Shuxin Yao, Yanbing Zheng, Manman Cui, Rong Yin, Guoqiang Han, Jin Hu, Chengyi Wang,
et al.
levels of total and oxidized glutathione were quantified with the
GSH/GSSG‐Glo Assay
Kit (
Promega
, Cat#
V6611
). The experiment was performed in white 96‐well plates at room temperature. Briefly, cells were plated
(10)
Pseudogene-Derived Long Noncoding RNAs GSTM3P1/Gstm2-ps1 Exacerbate Sepsis-Associated Acute Kidney Injury by Suppressing Their Parent Gene Translation
The American Journal of Pathology
May 1, 2026
Jing Huang, Zheng Dong, Qingqing Wei
glutathione ratio was measured using a
luminescence
-based glutathione
assay
kit (
Promega
, Madison, WI;
V6611
) following the manufacturer’s instruction. The intracellular reactive oxygen species (ROS) levels were
(11)
Senescent Stroma-Derived Glutamine: A Driver of Aggressiveness in Prostate and Ovarian Cancer Cells
Cells
April 24, 2026
Giulia Lori, Caterina Mancini, Caterina Paffetti, Dayana Desideri, Erica Pranzini, Alice Santi, Manuela Leri, Alessio Biagioni, Matteo Benelli, Pietro Spatafora,
et al.
(GSSG) glutathione levels were measured using the
GSH/GSSG-Glo™ Assay
(
Promega
, Fitchburg, WI, USA
V6611
), according to the manufacturer’s instructions. Briefly, cells were lysed and total glutathione (GSH
(12)
Stress adaptation pathways and HA–CD44 signaling maintain the survival of pancreatic cancer cells with centrosome amplification
Cell Communication and Signaling
April 7, 2026
Selahattin Can Ozcan, Evrim Goksel, Batuhan Mert Kalkan, Enes Cicek, Beste Kanevetci, Ceyda Acilan
assays
Intracellular reduced and oxidized glutathione levels were quantified using the
GSH/GSSG-Glo assay
(
Promega
,
V6611
) according to the manufacturer’s instructions. Control and
centrosome
-amplified (5d) cells were seeded
(13)
ZNF395 is a Hypoxia-Responsive Regulator of Mitochondrial Glutaminolysis in Clear Cell Renal Cell Carcinoma
Cancer Res
April 2, 2026
Joanna Koh, Chengheng Liao, Michelle Shu Wen Ng, Jing Han Hong, Hong Lee Heng, Dan Y. Gui, Zhenxun Wang, Benjamin Yan-Jiang Chua, Zhimei Li, Radoslaw M. Sobota,
et al.
without doxycycline (0.10 μg/mL), on the day prior to carrying out the
GSH/GSSG-Glo™ assay
(
Promega
V6611
). Cells were lysed with either Total Glutathione Lysis Reagent to
measure
total GSH level or Oxidised
(14)
Methionine metabolism is linked with phospholipid and glutamine metabolism to drive ferroptosis
Cell Reports
April 1, 2026
Jong Woo Kim, Seo Young Jang, Yeon Jin Roh, Yeajin Ju, Ju-Bin Kang, Byung-Sun Park, Ji-Yoon Lee, Min Wook Kim, Byung Jun Jeong, Woosung Jung,
et al.
cell culture
plates (
Corning
,
3596
). Glutathione levels were measured using a
GSH/GSSG-Glo Assay
Kit (
Promega
,
V6611
), and the experiments were carried out in accordance with the manufacturer’s protocol. To ensure that
(15)
Monophosphoryl lipid A boosts macrophage antimicrobial immunity by metabolically regulating source-specific ROS generation
Frontiers in Immunology
March 30, 2026
Dan Hao, Benjamin D. Klein, Margaret A. McBride, Julia K. Bohannon, Naeem K. Patil, Xenia D. Davis, Mary A. Oliver, Mei Lin Ning Dye, Sara Weidenbach, Jamey D. Young,
et al.
reader. GSH/GSSG quantification GSH and GSSG levels were measured using the
GSH/GSSG-Glo™ Assay
(
Promega
,
V6611
) according to the manufacturer’s protocol. ATP quantification Cells (5 ×105) were lysed with Luciferase
(16)
Microplastic-Induced Macrophage Dysfunction Drives Lung Tumor Progression through Glutathione Imbalance
ACS Nano
February 24, 2026
Bora Kim, Koung-Min Park, Haerang Lee, Young-Min Hyun
polystyrene MPs. GSH and GSSG levels were quantified using a
luminescence
-based
GSH/GSSG-Glo assay
(
Promega
,
V6611
) according to the manufacturer’s instructions.
Luminescence
-based measurements of the GSH/GSSG ratio
(17)
Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosis
Immunity
February 24, 2026
Ana C. Codo, Jesus E. Romero-Pichardo, Zhaoquan Wang, Mariano A. Aufiero, Tomi Lazarov, Waleska Saitz Rojas, Nicole S. Walker, Achuth Nair, Roger F. Cole, Savannah Adkins,
et al.
Kit,Invitrogen,Cat#EEA003 Alanine Aminotransferase (ALT/GPT) Activity Kit,Invitrogen,Cat#EEA001
GSH/GSSG-Glo Assay
,
Promega
,Cat#
V6611
NADP/NADPH-Glo Assay
,
Promega
,Cat#
G9081
Seahorse XF Glycolytic Rate
Assay
Kit,Agilent Technologies,Cat#103344-100 Seahorse
(18)
Enhancing 5-fluorouracil efficacy in colorectal cancer by inhibiting glutathione antioxidant mechanisms with an xCT inhibitor
Scientific Reports
February 22, 2026
Senanur Malcanlı, Remzi Okan Akar, Engin Ulukaya, Ali Burak Özkaya, Öykü Gönül Geyik
levels, the amount of GSH/GSSG was determined in
HCT-116 cells
. “
GSH/GSSG Glo Assay
(
Promega
CAT NO:
V6611
)” was used in accordance with the manufacturer’s instructions to determine the GSH/GSSG levels in CRC
(19)
Ferroptosis induces heterogeneous death profiles that are controlled by lysosome rupture
Developmental Cell
February 17, 2026
Jyotirekha Das, Saloni K. Hombalkar, Alison D. Klein, Esraa Nsasra, Muskaan Vasandani, Kay Petruzzi, Dajun Lu, Stephen Ruiz, Orit Kliper-Gross, Jiachen Hu,
et al.
(
S11368
;
Molecular Probes
) was used at a concentration of 15nM for all experiments.
GSH/GSSG-Glo™ Assay
(
V6611
;
Promega
) was used as per manufacturer’s guidelines. The GFP-Galectin-3, TFEB-GFP, and H2B-mCherry constructs
(20)
GPX4 Inhibitor Resistance and Metastatic Features in Triple‐Negative Breast Cancer
Advanced Science
February 17, 2026
Marie Sabatier, Mayher Kaur, Milena Chaufan, Felix‐Levin Hormann, Luiza Martins Nascentes Melo, Mario Palma, Jordan Torpey, Yanshan Liang, Alanis Carmona, Cameron Fraser,
et al.
Measurements NADP+/NADPH and GSH/GSSG ratios were assessed using NADP/NADPH‐Glo (Cat#
G9081
) and GSH/GSSG‐Glo (Cat#
V6611
)
assays
purchased from
Promega
. These bioluminescence‐based
assays
rely on the proportional relationship
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Reviews
Write a Review
(1)
12/16/2021
Good for GSH/GSSG Ratio Detection Assay Kit
Kumar Subramani
Reliable product.
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GSH/GSSG-Glo Assay from Promega
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