GSH/GSSG-Glo Assay V6611 from Promega

Product Specs
ItemGSH/GSSG-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV6611
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Monophosphoryl lipid A boosts macrophage antimicrobial immunity by metabolically regulating source-specific ROS generationFrontiers in ImmunologyMarch 30, 2026Dan Hao, Benjamin D. Klein, Margaret A. McBride, Julia K. Bohannon, Naeem K. Patil, Xenia D. Davis, Mary A. Oliver, Mei Lin Ning Dye, Sara Weidenbach, Jamey D. Young, et al.reader. GSH/GSSG quantification GSH and GSSG levels were measured using the GSH/GSSG-Glo™ Assay (Promega, V6611) according to the manufacturer’s protocol. ATP quantification Cells (5 ×105) were lysed with Luciferase

(2) Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosisImmunityFebruary 24, 2026Ana C. Codo, Jesus E. Romero-Pichardo, Zhaoquan Wang, Mariano A. Aufiero, Tomi Lazarov, Waleska Saitz Rojas, Nicole S. Walker, Achuth Nair, Roger F. Cole, Savannah Adkins, et al.Kit,Invitrogen,Cat#EEA003 Alanine Aminotransferase (ALT/GPT) Activity Kit,Invitrogen,Cat#EEA001 GSH/GSSG-Glo Assay,Promega,Cat#V6611 NADP/NADPH-Glo Assay,Promega,Cat#G9081 Seahorse XF Glycolytic Rate Assay Kit,Agilent Technologies,Cat#103344-100 Seahorse

(3) Microplastic-Induced Macrophage Dysfunction Drives Lung Tumor Progression through Glutathione ImbalanceACS NanoFebruary 24, 2026Bora Kim, Koung-Min Park, Haerang Lee, Young-Min Hyunpolystyrene MPs. GSH and GSSG levels were quantified using a luminescence-based GSH/GSSG-Glo assay (Promega, V6611) according to the manufacturer’s instructions. Luminescence-based measurements of the GSH/GSSG ratio

(4) Enhancing 5-fluorouracil efficacy in colorectal cancer by inhibiting glutathione antioxidant mechanisms with an xCT inhibitorScientific ReportsFebruary 22, 2026Senanur Malcanlı, Remzi Okan Akar, Engin Ulukaya, Ali Burak Özkaya, Öykü Gönül Geyiklevels, the amount of GSH/GSSG was determined in HCT-116 cells. “GSH/GSSG Glo Assay (Promega CAT NO: V6611)” was used in accordance with the manufacturer’s instructions to determine the GSH/GSSG levels in CRC

(5) Ferroptosis induces heterogeneous death profiles that are controlled by lysosome ruptureDevelopmental CellFebruary 17, 2026Jyotirekha Das, Saloni K. Hombalkar, Alison D. Klein, Esraa Nsasra, Muskaan Vasandani, Kay Petruzzi, Dajun Lu, Stephen Ruiz, Orit Kliper-Gross, Jiachen Hu, et al.(S11368; Molecular Probes) was used at a concentration of 15nM for all experiments. GSH/GSSG-Glo™ Assay (V6611; Promega) was used as per manufacturer’s guidelines. The GFP-Galectin-3, TFEB-GFP, and H2B-mCherry constructs

(6) GPX4 Inhibitor Resistance and Metastatic Features in Triple‐Negative Breast CancerAdvanced ScienceFebruary 17, 2026Marie Sabatier, Mayher Kaur, Milena Chaufan, Felix‐Levin Hormann, Luiza Martins Nascentes Melo, Mario Palma, Jordan Torpey, Yanshan Liang, Alanis Carmona, Cameron Fraser, et al.Measurements NADP+/NADPH and GSH/GSSG ratios were assessed using NADP/NADPH‐Glo (Cat#G9081) and GSH/GSSG‐Glo (Cat#V6611) assays purchased from Promega. These bioluminescence‐based assays rely on the proportional relationship

(7) A genetically encoded bifunctional enzyme mitigates redox imbalance and lipotoxicityNature MetabolismFebruary 16, 2026Xingxiu Pan, Subrata Munan, Austin L. Zuckerman, Andrew Pon, Niklas B. Thompson, Rasangi M. Perera, Nirajan Shrestha, Sara Violante, Justin R. Cross, Russell P. Goodman, et al.Glutathione Lysis Reagent or Oxidized Glutathione Lysis Reagent from the GSH/GSSG-Glo Assay kit (Promega, V6611) and samples were processed as previously described7. Live cell imaging using biosensors SoNar and

(8) Semaglutide Protects Retinal Ganglion Cells Against Rotenone-Induced Degeneration via Improved Glucose MetabolismInvest Ophthalmol Vis SciFebruary 10, 2026Zaynab A. Mouhammad, James R. Tribble, Alan Nicol, Evgenia Andreopoulou, Mariana Y. García-Bermúdez, Blanca I. Aldana, Rupali Vohra, Miriam Kolko, Pete A. WilliamsGSH/GSSG ratio were quantified using the GSH/GSSG-Glo Assay (Promega Corporation, Madison, WI, USA; Cat. V6611/V6612). The assay was performed according to the manufacturer's instructions. For each sample, 25 µL

(9) ZNF395 Is a Hypoxia-Responsive Regulator of Mitochondrial Glutaminolysis in Clear Cell Renal Cell CarcinomaCancer ResFebruary 4, 2026Joanna Koh, Chengheng Liao, Michelle Shu Wen Ng, Jing Han Hong, Hong Lee Heng, Dan Y. Gui, Zhenxun Wang, Benjamin Yan-Jiang Chua, Zhimei Li, Radoslaw M. Sobota, et al.without doxycycline (0.10 μg/mL), on the day prior to carrying out the GSH/GSSG-Glo™ assay (Promega V6611). Cells were lysed with either Total Glutathione Lysis Reagent to measure total GSH level or Oxidised

(10) The origin of hepatocellular carcinoma depends on metabolic zonationScienceJanuary 29, 2026Jason Guo, Roger Liang, Andrew Chung, Zhijie Li, Boyuan Li, Eric Chen, Lin Li, Jingjing Wang, Meng-Hsiung Hsieh, Ivy Xiangyi Fang, et al.liproxstatin-1. After 6 hours, GSH/GSSG was assessed using the GSH/GSSG-Glo assay kit (Promega Corporation #V6611) according to the manufacturer’s instructions. Statistics GraphPad Prism (version 10.0.2) software

(11) Dual targeting of BRAFV600E and ferroptosis results in synergistic anticancer activity via iron overload and enhanced oxidative stressJournal of Experimental & Clinical Cancer ResearchJanuary 7, 2026Jiangnan Hu, Chandrayee Ghosh, Tejinder P. Khaket, Zhongyue Yang, Yasmine Tabdili, Eden D. Alamaw, Myriem Boufraqech, Scott J. Dixon, Electron KebebewGSH and GSSG were assessed using a commercial GSH/GSSG assay kit (Promega, Madison, Wisconsin, USA, V6611), according to the manufacturer’s protocol. FerroOrange assay ATC cells were seeded in 96-well black-bottom

(12) Systemic control of HNF1B-driven redox homeostasis by N 6-adenosine methylationNucleic Acids ResearchJanuary 6, 2026Minji Park, Hwa-Ryeon Kim, Ji Hoon Park, YongHwan Kim, June-Ha Shin, Jueun Lee, Yeajin Ju, Geum-Sook Hwang, Dong-Myung Shin, Mi-Young Kim, et al.using QC samples. In vitro and in situ cellular glutathione measurement The GSH/GSSG-Glo assay (Cat# V6611, Promega) was performed according to the manufacturer’s instructions. Cells were lysed with 50 µl of

(13) PR inhibition stimulates G6PD expression to enhance malignancy in luminal breast cancerCell Death & DiseaseDecember 21, 2025Jae Woong Jeong, Janghee Lee, Soong June Bae, Yoon Jin Cha, Sungsoon Fang, Hae-kyung Lee, Sung Gwe AhnNADPH). GSH/GSSG assay The ratio of GSH/GSSG was measured using GSH/GSSG-GloTM Assay Kit (Promega, V6611). Briefly, the levels of total glutathione (GSH + GSSG) and oxidized glutathione (GSSG) were quantified

(14) Adaptation of redox metabolism in drug-tolerant persister cells is a vulnerability to prevent relapse in pancreatic cancerOncogenesisDecember 9, 2025Nadine Abdel Hadi, Gabriela Reyes-Castellanos, Tristan Gicquel, Scarlett Gallardo-Arriaga, Emma Cosialls, Emeline Boet, Jean-Emmanuel Sarry, Rawand Masoud, Juan Iovanna, Alice Carrier*100. Quantification of small antioxidant molecules GSH/GSSG measurement GSH/GSSG-Glo Assay kit (Promega, V6611) was used following manufacturer’s protocol with some modifications. Briefly, 10 mg of tumor tissue

(15) Protein CoAlation is regulated by and integrated with growth factor signalling and the cellular antioxidant responseRedox BiolDecember 4, 2025Donagh Gribbon, Arnau Garcíal Salmerón, Ivan Gout, Rosemary O'Connorglutathione levels Total Glutathione levels were measured using the GSH/GSSG Glo assay (Promega Corporation, V6611), according to the manufacturer's protocol. Briefly, cells were seeded in 96 well plates and treated

(16) The cell cycle regulator PLK1 promotes murine melanoma progression by regulating the transcription factor BACH1PLOS BiologyNovember 24, 2025Fengyi Mao, Sai Wu, Derek B. Allison, Daheng He, Yifan Kong, Chaohao Li, Zhiguo Li, Yanquan Zhang, Xinyi Wang, Qiongsi Zhang, et al.the indicated drugs for 48 h. GSH/GSSG levels were measured using the Promega GSH/GSSG-Glo Assay kit (V6611) according to the manufacturer’s instructions. Nuclear extract assay The melanoma cells were seeded

(17) Atovaquone-induced oxidative stress activates the pentose phosphate pathway and Immunogenic cell death in ovarian cancerScientific ReportsNovember 24, 2025Mayra Betancourt Ponce, Nicha Boonpattrawong, Alexander J. Fagan, Michael Newton, Manish Patankar, Lisa Barroilhet(GSH) and glutathione disulfide (GSSG) ratios were measured via the GSH/GSSG-Glo™ Assay (Promega, #V6611). OVCAR3 and OVCAR5 cells were seeded in 96-well plates and incubated overnight. The cells were then

(18) Redox destabilization by ibrutinib promotes ferroptosis in diffuse large B-cell lymphoma (DLBCL)Cell Death DiscoveryOctober 31, 2025Anuschka Langpape, Debora Bonasera, Jenny Stroh, Moritz Reese, Maria Cartolano, Gianmaria Liccardi, Silvia von Karstedtquantification To quantify concentrations of cellular GSH and GSSG, the GSH/GSSG Glo® Assay (Promega, V6611) was used according to the manufacturer’s instructions using 5 × 104 cells per well cells. MCB assay For

(19) The essential host genome for Cryptosporidium survival exposes metabolic dependencies that can be leveraged for treatmentCellOctober 16, 2025N. Bishara Bishara Marzook, Ok-Ryul Song, Lotta Baumgärtel, Netanya Bernitz, Tapoka T. Mkandawire, Lucy C. Watson, Vanessa Nunes, Scott Warchal, James I. MacRae, Michael Howell, et al.Green,Invitrogen,Cat# C10444 CellROX™ Deep Red,Invitrogen,Cat# C10422 GSH/GSSG-Glo™ assay,Promega,Cat# V6611 NanoGlo Luciferase kit,Promega,Cat# N1150 RNeasy Mini kit,Qiagen,Cat# 74104 Watchmaker mRNA Library Prep kit

(20) Non-canonical dihydrolipoyl transacetylase promotes chemotherapy resistance via mitochondrial tetrahydrofolate signalingNat CommunOctober 8, 2025Jung Seok Hwang, JiHoon Kang, Jaehyun Kim, Kiyoung Eun, Sophia West, Hannah E. Bacho, Vanessa Avalos, Sydney Shuff, Dong M. Shin, Nabil F. Saba, et al.from Invitrogen. ROS-Glo H2O2 Assay (G8820), NADP/NADPH-Glo Assay (G9081), and GSH/GSSG-Glo Assay (V6611) were procured from Promega. MtioSOX (M36008) was obtained from Invitrogen. Acetyl-CoA assay (ab87546),

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12/16/2021Good for GSH/GSSG Ratio Detection Assay Kit Kumar SubramaniReliable product.

GSH/GSSG-Glo Assay

GSH/GSSG-Glo Assay from Promega

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GSH/GSSG-Glo Assay from Promega

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