GSH/GSSG-Glo Assay V6611 from Promega

Product Specs
ItemGSH/GSSG-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV6611
Quantity10ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Identification of a NUAK1/2 Inhibitor as a Macrophage-Polarizing Compound by a Machine Learning-Based Phenotypic Cell Painting ScreenInt J Mol SciAugust 14, 2026Johanna B. Brüggenthies-Brunner, Jakob Dittmer, Julia Sauer, Sarah Groetzner, Anika Liu, Eva Martin, Anja Teufel, Christine Strasser, Helga Bronner, Robert Ries, et al.corresponding figure legends. To quantify total glutathione ratios, a luminescent -based GSH/GSSG-Glo™ Assay (V6611, Promega, Walldorf, Germany) was performed following the manufacturer’s instructions for adherent cells

(2) Combinatorial Effects of Plasma-Treated Solution and Plasma-Treated Hydrogel with Cisplatin in Vulvar Cancer CellsOncology ResearchAugust 13, 2026Estelle C. I. D. Schad, Janet P. Raja Xavier, Hortense Decool, Marcel Arnholdt, Franziska Keßler, Jan Schöttke, Sara Y. Brucker, Ernst Oberlechner, Johanna Laupp, Martin Weiss(GSH/GSSG) Intracellular GSH and GSSG levels were measured using the GSH/GSSG-Glo™ assay (Promega, #V6611), following the supplier’s protocol. SW954 cells were subjected to the previously introduced combination

(3) Myocilin drives cardiomyocyte mitochondrial dysfunction via SLC3A2-dependent redox imbalance in heart failureRedox BiolAugust 12, 2026Xiaoyue Yu, Junxia Zhang, Junfang Wu, Mingming Zhao, Hongtu Cui, Sen Huang, Jiawen Liu, Minwen Long, Yandong Pei, Aiming Liu, et al.of total glutathione and oxidized glutathione (GSSG) were quantified using the GSH/GSSG-Glo™ Assay (V6611, Promega, USA). Briefly, AC16 cells were lysed with either Total or Oxidized Glutathione Lysis Reagent;

(4) Early-life oxidative stress programs persistent NFκB activation and neuroinflammation in autism spectrum disorderFront ImmunolAugust 10, 2026Yun Jiao, Qingzheng Jia, Xiaozhuang Zhang, Claire Zhang, Chongxia Wei, Dakota Wang, Xiaohan Liu, Huilin Li, Alex Hu, Liqin Zeng, et al.ratio, indicating glutathione redox status, was determined by the GSH/GSSG-Glo™ Assay Kit (Promega, V6611) as per supplier’s protocol. Malondialdehyde quantification MDA levels in plasma and tissues were

(5) Doxorubicin‐Induced Cytotoxicity in Adipose‐Derived Stem Cells Is Associated With Altered SAPK/JNK SignalingFASEB JJuly 30, 2026Aleksandra Skubis‐Sikora, Kinga Pogoda‐Mieszczak, Bartosz Sikora, Edyta Bogunia, Aleksandra Bryzek, Piotr Czekajperformed as six biological replicates (N = 6). 2.9.2 GSH/GSSG‐Glo Assay The GSH/GSSG‐Glo assay (V6611; Promega, Madison, WI, USA) was used to measure the levels of reduced glutathione (GSH), oxidized glutathione

(6) Targeting chordoma via an isocitrate dehydrogenase-1-dependent susceptibility to redox metabolismActa NeuropatholJuly 23, 2026Matthew Pun, Akash Deogharkar, Siva Kumar Natarajan, Nicholas Nuechterlein, Wentao Tian, Sunjong Ji, Eleanor Young, Fusheng Yang, Debra Hawes, Anthony C. Andren, et al.inhibitor at 3 μM for 24 or 48 h. Subsequently, cells were processed with the GSH/GSSG-Glo Assay (Promega, V6611) which quantifies total and oxidized glutathione. Ratios were calculated from luminescent intensity

(7) A targetable FTO/SLC7A11/CBS/CTH axis controls cysteine metabolism, growth and survival in NSCLCSci AdvJuly 3, 2026Nishanth Kuganesan, Margaret Pan, Haowen Jiang, Stavros Melemenidis, Jiangbin Ye, Sara Richter, Maximillian Diehn, Kerriann M. Casey, Edward E. Graves, Quynh Thu Le, et al.following day, the GSH/GSSG ratio and total GSH were determined using GSH/GSSG-Glo (Promega, catalog no. V6611) according to the manufacturer’s instructions. For pharmacological inhibition of FTO, H1299 (10,000

(8) Metabolic plasticity and optimal redox homeostasis are essential for efficient metastatic colonizationMolecular MetabolismJuly 1, 2026Ece Grace, Deyu Zou, Romy Böttcher-Loschinski, Martin Böttcher, Harald Schuhwerk, Yussuf Hajjaj, Annemarie Schwab, Simon Brandt, Ana Clavel Ezquerra, Witold Szymanski, et al.oxidized glutathione (GSH and GSSG) levels were determined using the GSH/GSSG-Glo™ Assay (Promega, V6611), following the manufacturer’s instructions. Standard curves were generated using purified NAD (Sigma–Aldrich,

(9) Ferrostatin-1 and hinokitiol supplementation enhance human hematopoietic stem cell expansion in a chemically defined mediumMolecular Therapy AdvancesJune 11, 2026Lushen Li, Pankaj K. MandalCellTiter-Glo Luminescent Cell Viability Assay kit (Catalog #G7571) and GSH/GSSG-Glo assay reagent (Catalog #V6611) were purchased from Promega (Madison, WI). Iscove’s modified Dulbecco’s medium (IMDM, Catalog # 12440053),

(10) Nickel exposure disrupts epigenetic repression of developmental genes in mouse embryonic stem cellsToxicology and Applied PharmacologyJune 1, 2026Beatrix Bradford, David Filipovic, Xiaoru Zhang, Viktoriia Goriainova, Suresh Cuddapahratio of reduced (GSH) to oxidized glutathione (GSSG) was determined using the GSH/GSSG-Glo™ Assay Kit (V6611, Promega) according to the manufacturer’s protocol. Statistical significance was assessed using a t-test. 2.4.

(11) Non-invasive radiogenomic mapping of the SMARCAL1-driven ferroptotic niche is associated with longitudinal MRD-negative surveillance in early-stage NSCLCFrontiers in ImmunologyMay 25, 2026Zehao Huang, JunDao He, Yue Li, ZhanYu Xu, Huajian Peng, Xiang Gao, HuaFu Zhou, JianJi Guo, Nuo YangCayman Chemical 10009055, normalized to protein), reduced glutathione (GSH/GSSG-Glo Assay, Promega V6611, normalized to cell count), and transmission electron microscopy for shrunken mitochondria with increased

(12) Inflammatory CD4+ T cells can waive NRF2-dependent SLC7A11-mediated cystine uptake by using ASCT1iScienceMay 15, 2026Christopher Thomas Neullens, Sudheendra Hebbar Subramanyam, Gerd Horneff, Tilmann Kallinich, Freya Huijsmans, Jorg van Loosdregt, Bas Vastert, Klaus Tenbrock, Kim Ohlmeasurement and the calculation of GSH/GSSG ratio was conducted according to the manufacturer’s instructions (V6611, Promega). Quantification and statistical analysis Flow cytometric analysis and gating was performed

(13) PIEZO1 is Required for Acute Myeloid Leukemia Progression and Leukemia Stem Cell Maintenance via HIF1A‐SLC7A11 Axis‐Mediated Ferroptosis DefenseAdvanced ScienceMay 10, 2026Tiantian Zhang, Ziyan Cao, Kexin Gao, Shuxin Yao, Yanbing Zheng, Manman Cui, Rong Yin, Guoqiang Han, Jin Hu, Chengyi Wang, et al.levels of total and oxidized glutathione were quantified with the GSH/GSSG‐Glo Assay Kit (Promega, Cat# V6611). The experiment was performed in white 96‐well plates at room temperature. Briefly, cells were plated

(14) Pseudogene-Derived Long Noncoding RNAs GSTM3P1/Gstm2-ps1 Exacerbate Sepsis-Associated Acute Kidney Injury by Suppressing Their Parent Gene TranslationThe American Journal of PathologyMay 1, 2026Jing Huang, Zheng Dong, Qingqing Weiglutathione ratio was measured using a luminescence-based glutathione assay kit (Promega, Madison, WI; V6611) following the manufacturer’s instruction. The intracellular reactive oxygen species (ROS) levels were

(15) Senescent Stroma-Derived Glutamine: A Driver of Aggressiveness in Prostate and Ovarian Cancer CellsCellsApril 24, 2026Giulia Lori, Caterina Mancini, Caterina Paffetti, Dayana Desideri, Erica Pranzini, Alice Santi, Manuela Leri, Alessio Biagioni, Matteo Benelli, Pietro Spatafora, et al.(GSSG) glutathione levels were measured using the GSH/GSSG-Glo™ Assay (Promega, Fitchburg, WI, USA V6611), according to the manufacturer’s instructions. Briefly, cells were lysed and total glutathione (GSH

(16) Stress adaptation pathways and HA–CD44 signaling maintain the survival of pancreatic cancer cells with centrosome amplificationCell Communication and SignalingApril 7, 2026Selahattin Can Ozcan, Evrim Goksel, Batuhan Mert Kalkan, Enes Cicek, Beste Kanevetci, Ceyda Acilanassays Intracellular reduced and oxidized glutathione levels were quantified using the GSH/GSSG-Glo assay (Promega, V6611) according to the manufacturer’s instructions. Control and centrosome-amplified (5d) cells were seeded

(17) ZNF395 is a Hypoxia-Responsive Regulator of Mitochondrial Glutaminolysis in Clear Cell Renal Cell CarcinomaCancer ResApril 2, 2026Joanna Koh, Chengheng Liao, Michelle Shu Wen Ng, Jing Han Hong, Hong Lee Heng, Dan Y. Gui, Zhenxun Wang, Benjamin Yan-Jiang Chua, Zhimei Li, Radoslaw M. Sobota, et al.without doxycycline (0.10 μg/mL), on the day prior to carrying out the GSH/GSSG-Glo™ assay (Promega V6611). Cells were lysed with either Total Glutathione Lysis Reagent to measure total GSH level or Oxidised

(18) Methionine metabolism is linked with phospholipid and glutamine metabolism to drive ferroptosisCell ReportsApril 1, 2026Jong Woo Kim, Seo Young Jang, Yeon Jin Roh, Yeajin Ju, Ju-Bin Kang, Byung-Sun Park, Ji-Yoon Lee, Min Wook Kim, Byung Jun Jeong, Woosung Jung, et al.cell culture plates (Corning, 3596). Glutathione levels were measured using a GSH/GSSG-Glo Assay Kit (Promega, V6611), and the experiments were carried out in accordance with the manufacturer’s protocol. To ensure that

(19) Monophosphoryl lipid A boosts macrophage antimicrobial immunity by metabolically regulating source-specific ROS generationFrontiers in ImmunologyMarch 30, 2026Dan Hao, Benjamin D. Klein, Margaret A. McBride, Julia K. Bohannon, Naeem K. Patil, Xenia D. Davis, Mary A. Oliver, Mei Lin Ning Dye, Sara Weidenbach, Jamey D. Young, et al.reader. GSH/GSSG quantification GSH and GSSG levels were measured using the GSH/GSSG-Glo™ Assay (Promega, V6611) according to the manufacturer’s protocol. ATP quantification Cells (5 ×105) were lysed with Luciferase

(20) Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosisImmunityFebruary 24, 2026Ana C. Codo, Jesus E. Romero-Pichardo, Zhaoquan Wang, Mariano A. Aufiero, Tomi Lazarov, Waleska Saitz Rojas, Nicole S. Walker, Achuth Nair, Roger F. Cole, Savannah Adkins, et al.Kit,Invitrogen,Cat#EEA003 Alanine Aminotransferase (ALT/GPT) Activity Kit,Invitrogen,Cat#EEA001 GSH/GSSG-Glo Assay,Promega,Cat#V6611 NADP/NADPH-Glo Assay,Promega,Cat#G9081 Seahorse XF Glycolytic Rate Assay Kit,Agilent Technologies,Cat#103344-100 Seahorse

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12/16/2021Good for GSH/GSSG Ratio Detection Assay Kit Kumar SubramaniReliable product.

GSH/GSSG-Glo Assay

GSH/GSSG-Glo Assay from Promega

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GSH/GSSG-Glo Assay from Promega

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