HDAC-Glo I/II Assay G6420 from Promega

Product Specs
ItemHDAC-Glo I/II Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG6420
Quantity10ml
Molecular Weight~61kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) New potent N-hydroxycinnamamide-based histone deacetylase inhibitors suppress proliferation and trigger apoptosis in THP-1 leukaemia cellsArch Pharm (Weinheim)April 1, 2025Magdalena Onuscakova, Tereza Kauerova, Eva Fialova, Hana Pizova, Vladimir Garaj, Miroslav Kemka, Vladimir Frecer, Peter Kollar, Pavel Bobalagainst enzyme activity of HDAC class I and II were determined using HDAC‐Glo™ I/II Assay (Cat. #: G6420; Promega Corporation) according to the manufacturer's instructions. THP‐1 cells were seeded into a white‐walled

(2) Pharmacological manipulation of liver fibrosis progression using novel HDAC6 inhibitorsThe FEBS JournalMarch 14, 2025Maria Teresa Borrello, Dusan Ruzic, Hannah Paish, Eleanor Graham, Amy L. Collins, Rebecca Scott, Sam Higginbotham, Branko Radovic, Glyn Nelson, David Bulmer, et al.assay Deacetylase activity of compounds DR2 and FDR2 was measured using the HDAC‐Glo I/II assay kit (G6420) (Promega, Madison, WI, USA). Briefly, compounds DR‐3 and FDR2 were serially diluted in HDAC‐Glo™ I/II

(3) HDAC7 is a potential therapeutic target in acute erythroid leukemiaLeukemiaDecember 1, 2024Wenyu Zhang, Keita Yamamoto, Yu-Hsuan Chang, Tomohiro Yabushita, Yangying Hao, Ruka Shimura, Jakushin Nakahara, Shiori Shikata, Kohei Iida, Qianyi Chen, et al.measure assay HDAC activity was evaluated using the HDAC-GloTM I/II Assay and Screening System (Promega #G6420). 1 × 104 cells/well were seeded in a 96-well plate with 100 μL HDAC-GloTM I/II buffer. Then, 100 μL

(4) The HDAC6 inhibitor AVS100 (SS208) induces a pro-inflammatory tumor microenvironment and potentiates immunotherapyScience AdvancesNovember 15, 2024Damian Kovalovsky, Satish Noonepalle, Manasa Suresh, Dileep Kumar, Michael Berrigan, Nithya Gajendran, Sumit Upadhyay, Anelia Horvath, Allen Kim, David Quiceno-Torres, et al.inhibition assay Cellular HDAC inhibitory activity was measured using HDAC-Glo I/II Assay (Promega, G6420) following the manufacturer’s protocol. Briefly, cells were plated at a density of 10,000 cells per

(5) HDAC6 as a Prognostic Factor and Druggable Target in HER2-Positive Breast CancerCancers (Basel)November 6, 2024Michela Cortesi, Sara Bravaccini, Sara Ravaioli, Elisabetta Petracci, Davide Angeli, Maria Maddalena Tumedei, William Balzi, Francesca Pirini, Michele Zanoni, Paola Possanzini, et al.endogenous genes GAPDH, ACTIN-B, and HPRT-1. 2.9. HDAC-Glo I/II Assay The HDAC-Glo I/II assay kit (G6420) was provided by Promega Corporation. Nexturastat A was purchased by Selleck Chemicals LLC (Houston,

(6) HDAC activity is dispensable for repression of cell-cycle genes by DREAM and E2F:RB complexesNature CommunicationsMay 24, 2024Alison K. Barrett, Manisha R. Shingare, Andreas Rechtsteiner, Kelsie M. Rodriguez, Quynh N. Le, Tilini U. Wijeratne, Corbin E. Mitchell, Miles W. Membreno, Seth M. Rubin, Gerd A. Müllerplate. HDAC activity of three technical replicates was measured using the HDAC-Glo I/II Assay (Promega, #G6420) following the manufacturer’s protocol on-bead with an Envision plate reader (Perkin Elmer). The remaining

(7) Neutrophil Elastase Degrades Histone Deacetylases and Sirtuin 1 in Primary Human Monocyte Derived MacrophagesInternational Journal of Molecular SciencesApril 12, 2024Shuo Zheng, Gamze B. Bulut, Apparao B. Kummarapurugu, Jonathan Ma, Judith A. Voynowcells were incubated with HDAC-Glo kit reagent mixture per the manufacturer’s instructions (Promega G6420 HDAC-Glo I/II assay, Madison, WI, USA), and luminescence was read using the SPARK plate reader (TECAN,

(8) Propionic acid promotes neurite recovery in damaged multiple sclerosis neuronsBrain communicationsJanuary 1, 2024Gisevius B, Duscha A, Poschmann G, Stühler K, Motte J, Fisse AL, Augustyniak S, Rehm A, Renk P, Böse C, et al.and BA for 30 min. Analysis of HDAC class I/II inhibition was examined via the HDAC-Glo™ I/II Assay (#G6420, Promega) per manufacturer’s instructions. Respective relative light units (RLUs) were measured by the

(9) Radiotherapy-induced Immune Response Enhanced by Selective HDAC6 InhibitionMolecular Cancer TherapeuticsDecember 1, 2023Satish Kumar R. Noonepalle, Scott Grindrod, Nima Aghdam, Xintang Li, Maria Gracia-Hernandez, Christian Zevallos-Delgado, Mira Jung, Alejandro Villagra, Anatoly Dritschilocells. HDAC inhibition was analyzed using the HDAC-Glo I/II Assay and Screening System (catalog no. G6420, Promega) following manufacturer’s protocol. Briefly, 10,000 cells/well were seeded in a 96-well white

(10) HDAC activity is dispensable for repression of cell-cycle genes by DREAM and E2F:RB complexesbioRxivNovember 3, 2023Alison Barrett, Manisha R. Shingare, Andreas Rechtsteiner, Tilini U. Wijeratne, Kelsie M. Rodriguez, Seth M. Rubin, Gerd A. Müllerplate. HDAC activity of three technical replicates was measured using the HDAC-Glo I/II Assay (Promega, #G6420) following the manufacturer’s protocol on-bead with an Envision plate reader (Perkin Elmer). The remaining

(11) Ovulatory signal-triggered chromatin remodeling in ovarian granulosa cells by HDAC2 phosphorylation activation-mediated histone deacetylationEpigenetics & ChromatinApril 19, 2023Jiamin Jin, Peipei Ren, Xiang Li, Yinyi Zhang, Weijie Yang, Yerong Ma, Mengru Lai, Chao Yu, Songying Zhang, Yin-Li Zhangindependent experiment. HDAC2 activity assay HDAC2 activity was measured with HDAC-Glo™ I/II Assays (G6420; Promega, USA) according to the manufacturer’s protocol. Briefly, 100,000 cells were mixed with HDAC-Glo™

(12) Medulloblastoma uses GABA transaminase to survive in the cerebrospinal fluid microenvironment and promote leptomeningeal disseminationCell reportsJune 29, 2021Martirosian V, Deshpande K, Zhou H, Shen K, Smith K, Northcott P, Lin M, Stepanosyan V, Das D, Remsik J, et al.protocol. For HDAC activity analysis, the HDAC-Glo™ I/II Assays and Screening System (Promega, Cat#G6420) was used. 1,000 D283 and D425 Scrambled, ABAT KD, and ABAT OE cells were plated into a collagen-coated

(13) Combining DNMT and HDAC6 inhibitors increases anti-tumor immune signaling and decreases tumor burden in ovarian cancerScientific reportsFebruary 26, 2020Moufarrij S, Srivastava A, Gomez S, Hadley M, Palmer E, Austin PT, Chisholm S, Roche K, Yu A, Li J, et al.enzymatic inhibition assay HDACi enzymatic function was measured using HDACglo class I/IIa Assay (Promega, G6420). Cells were seeded in a 96-well dish and treated with 500 nM 5-azacytidine and varying levels of HDACi

(14) The stem cell-associated transcription co-factor, ZNF521, interacts with GLI1 and GLI2 and enhances the activity of the Sonic hedgehog pathwayCell death & diseaseSeptember 26, 2019Scicchitano S, Giordano M, Lucchino V, Montalcini Y, Chiarella E, Aloisio A, Codispoti B, Zoppoli P, Melocchi V, Bianchi F, et al.dilutions were made for the assays and extracts incubated with the HDAC-Glo™ I/II substrate and developer (G6420, Promega) for luminescence reading with the GloMax Explorer luminometer in white 96-well plates. The

(15) Improved high-throughput quantification of luminescent microplate assays using a common Western-blot imaging systemMethodsXOctober 27, 2017Liam J. Hawkins, Kenneth B. Storeyskeletal muscle was determined using the commercially available HDAC-Glo I/II assay (Promega, Catalogue #G6420) with the above method, including well position effect corrections. Total soluble protein extracts were

(16) Novel analogs targeting histone deacetylase suppress aggressive thyroid cancer cell growth and induce re-differentiationCancer gene therapyAugust 1, 2015Jang S, Yu XM, Odorico S, Clark M, Jaskula-Sztul R, Schienebeck CM, Kupcho KR, Harrison AD, Winston-McPherson GN, Tang W, et al.target genes. Biochemical assay for inhibitory effects on selective HDACs The HDAC-Glo I/II assay kit (G6420) was provided by Promega Corporation (Madison, WI). Human recombinant C-ter-GST-HDAC 1 (H83-30G) and C-ter-HIS-HDAC

(17) Tumor-suppressor role of Notch3 in medullary thyroid carcinoma revealed by genetic and pharmacological inductionMolecular cancer therapeuticsFebruary 1, 2015Jaskula-Sztul R, Eide J, Tesfazghi S, Dammalapati A, Harrison AD, Yu XM, Scheinebeck C, Winston-McPherson G, Kupcho KR, Robers MB, et al.analysis as described below. Biochemical assay for AB3 characterization The HDAC-Glo™ I/II assay kit (G6420) was provided by Promega Corporation. Human recombinant C-ter-GST-HDAC 1 (H83-30G) and C-ter-HIS-HDAC

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HDAC-Glo I/II Assay

HDAC-Glo I/II Assay from Promega

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HDAC-Glo I/II Assay from Promega

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