Renilla-Glo Luciferase Assay System E2720 from Promega

Product Specs
ItemRenilla-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2720
Quantity100ml
Molecular Weight~70kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Precision-Engineered Dermatan Sulfate-Mimetic Glycopolymers for Multi-Targeted SARS-CoV-2 Inhibition †Mar DrugsDecember 18, 2025Lihao Wang, Lei Gao, Chendong Yang, Mengfei Yin, Jiqin Sun, Luyao Yang, Chanjuan Liu, Simon F. R. Hinkley, Guangli Yu, Chao Caimedium and incubated for an additional 48 h, and 15 μL Renilla luciferase reagent (Promega, cat lot #E2720) was added to the plate. The plates were placed on an orbital shaker for 2 min, followed by 10 min at

(2) Collateral hypersensitivity between ZY19489 and piperaquine neutralizes PfCRT-mediated drug efflux and Plasmodium falciparum resistanceResearch SquareOctober 9, 2025David Fidock, Tolla Okombo, Tarrick Ndiaye, Igor M. R. Qahash, Igor Gil-Iturbe, Laura M. Gil-Iturbe, Jessica L. Bridgford, Vinicius Bridgford, Vinicius Bonatto, Tomas Ward, et al.quantified at 0 and 72 hours after incubation using the Renilla-Glo(R) Luciferase Assay System (Promega E2750) and the GloMax® Discover Multimode Microplate Reader (Promega). The luminescence values in the knockdown

(3) Protocol for monitoring mRNA translation and degradation in human cell-free lysatesSTAR ProtocolsSeptember 19, 2025Nino Schwaller, Evangelos D. Karousiscommercial assays,Critical commercial assays ,, Renilla-Glo luciferase assay system,Promega,Cat. No. E2720 Maxwell RSC simplyRNA cells kit,Promega,Cat. No. AS1390 Monarch RNA cleanup kit,New England Biolabs,Cat.

(4) Plasmodium falciparum protein kinase 6 and hemozoin formation are inhibited by a type II human kinase inhibitor exhibiting antimalarial activityCell Chemical BiologyJuly 17, 2025Flore Nardella, Tiantian Jiang, Lushun Wang, Monica J. Bohmer, Subhoja Chakraborty, John Okombo, Jaeson Calla, Tatiane Macedo Silva, Samuel Pazicky, Jianwei Che, et al.N2410 Nano-Glo Hibit Lytic system,Promega,Cat# N3030 Renilla-Glo(R) Luciferase Assay System,Promega,Cat# E2750 Deposited data,, Compound 12-selected resistance line sequencing,NCBI Sequence Read Archive,https://www.ncbi.nlm.nih.gov/sra/Accession#PRJNA1147332 Mass spectrometry

(5) Efficient cell-free translation from diverse human cell typesJournal of Biological ChemistryJuly 1, 2025Jana Ziegelmüller, Nikolaos Kouvelas, Nino Schwaller, Priyanka Thambythurai, Alexander M. Hofer, Oliver Mühlemann, Evangelos D. Karousishour. Luciferase assay Luciferase assays were performed using the Renilla-Glo system (Promega, Cat No. E2720). In vitro translation reactions were combined with 1x Renilla-Glo substrate in Renilla-Glo buffer in

(6) ANP32 proteins from ticks and vertebrates are key host factors for replication of Bourbon virus across speciesJournal of VirologyJune 17, 2025Zhenyu Zhang, Ishmael D. Aziati, Thomas Nipper, Adrianus C. M. Boon, Andrew MehleE1500). Renilla luciferase (RLuc) activity was quantified with the Renilla-Glo Luciferase Assay (Promega E2720). FFLuc was normalized to RLuc within each sample. The expression levels of polymerase proteins in different

(7) Engineered SH3-Derived Sherpabodies Function as a Modular Platform for Targeted T-cell ImmunotherapyCancer ResearchMay 15, 2025Rogelio A. Hernández-López, Tapio Kesti, Anna R. Mäkelä, Zhe Zhao, Wei Yu, Yurie Tonai, Hector J. Monzo, Kerttu Kalander, Sirpa Leppä, Päivi M. Ojala, et al.incubator, the remaining luciferase activity was measured using Renilla-Glo Luciferase Assay System (E2720, Promega, Madison, USA) and Promega GloMax Navigator Microplate Luminometer. Measurement of cytokine

(8) Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profilesThe Journal of clinical investigationMarch 17, 2025Weiss S, Lin HM, Acosta E, Komarova NL, Chen P, Wodarz D, Baine I, Duerr R, Wajnberg A, Gervais A, et al.lysis buffer, and processed for detection of Renilla luciferase (Renilla-Glo Luciferase Assay, Promega, E2720) in black-walled 96-well plates (Greiner, 655096). A Cytation3 apparatus (BioTek) using Gen5 software

(9) Purine but Not Pyrimidine De Novo Nucleotide Biosynthesis Inhibitors Strongly Enhance the Antiviral Effect of Corresponding Nucleobases Against Dengue VirusMoleculesJanuary 7, 2025Laurent F. Bonnac, Christine D. Dreis, Madhu Rai, Robert J. Geraghtythree days. The cells were the washed with PBS and Complete Promega Renilla reagent was added (Promega E2720, Madison, USA, 1 µL Renilla-Glo Substrate + 99 µL Renilla-Glo Buffer + 100 µL phenol-red-free DMEM supplemented

(10) Adaptive Multi-Epitope Targeting and Avidity-Enhanced (AMETA) Nanobody Platform: Leveraging Diverse Mechanisms for Ultrapotent, Durable Antiviral TherapyCellNovember 27, 2024Yufei Xiang, Jialu Xu, Briana L. McGovern, Anna Ranzenigo, Wei Huang, Zhe Sang, Juan Shen, Randy Diaz-tapia, Ngoc Dung Pham, Abraham J.P. Teunissen, et al.Kits,Qiagen,12362 ExpiFectamine 293 kit,Thermo Fisher,A14635 Renilla-Glo luciferase assay system,Promega,E2720 Mycoplasma Detection Kit,InvivoGen,rep-mys-20 Deposited data,Deposited data,Deposited data "Structure,

(11) Viral hijacking of hnRNPH1 unveils a G-quadruplex-driven mechanism of stress controlCell Host & MicrobeSeptember 11, 2024Philipp Schult, Beate Mareike Kümmerer, Markus Hafner, Katrin Paeschke"Rabbit Reticulocyte Lysate System, Nuclease Treated",Promega,Cat# L4960 Renilla-Glo® Luciferase Assay System,Promega,Cat# E2720 RNAScope Multiplex ISH kit v1,ACDbio,Cat# 320850 SuperScript™ III First-Strand Synthesis System,Thermo Fisher Scientific

(12) SARS-CoV-2-related bat viruses evade human intrinsic immunity but lack efficient transmission capacityNature microbiologyAugust 1, 2024Peña-Hernández MA, Alfajaro MM, Filler RB, Moriyama M, Keeler EL, Ranglin ZE, Kong Y, Mao T, Menasche BL, Mankowski MC, et al.normalized to GFP transfected cells at 24 hpi by using Renilla-Glo Luciferase Assay system (Promega Cat. E2750). For IFN assays, Vero-ACE2/TMPRSS2 cells were pre-treated with 1000 U/mL of Universal IFNα (#11200–2)

(13) Antiplasmodial peptaibols act through membrane directed mechanismsCell Chemical BiologyFebruary 15, 2024Jennifer E. Collins, Jin Woo Lee, Frances Rocamora, Gagandeep S. Saggu, Karen L. Wendt, Charisse Flerida A. Pasaje, Sebastian Smick, Natalia Mojica Santos, Raphaella Paes, Tiantian Jiang, et al.at 0 and 72 h by quantitating luminescence using the Renilla-Glo(R) Luciferase Assay System (Promega E2750) and the GloMax® Discover Multimode Microplate Reader (Promega). Parasite growth was determined from

(14) Reaction hijacking inhibition of Plasmodium falciparum asparagine tRNA synthetaseNature CommunicationsJanuary 31, 2024Stanley C. Xie, Yinuo Wang, Craig J. Morton, Riley D. Metcalfe, Con Dogovski, Charisse Flerida A. Pasaje, Elyse Dunn, Madeline R. Luth, Krittikorn Kumpornsin, Eva S. Istvan, et al.signals were taken at 0 and 72 h post-invasion using the Renilla-Glo(R) Luciferase Assay System (Promega E2750) and the GloMax® Discover Multimode Microplate Reader (Promega). The luminescence values in the knockdown

(15) Multimodal, broadly neutralizing antibodies against SARS-CoV-2 identified by high-throughput native pairing of BCRs from bulk B cellsCell chemical biologyNovember 16, 2023Keitany GJ, Rubin BER, Garrett ME, Musa A, Tracy J, Liang Y, Ebert P, Moore AJ, Guan J, Eggers E, et al.free)",Biolegend,Cat#589106 BioGlo Luciferase,Promega,Cat#G7941 Renilla-Glo luciferase,Promega,Cat#E2720 PKH67 ethanolic dye solution,Sigma Adrich,Cat#P7333 SARS-CoV2 Spike Trimer ECD-His,Genscript,N/A SARS-CoV2

(16) Universal features of Nsp1-mediated translational shutdown by coronavirusesbioRxivJune 13, 2023Katharina Schubert, Evangelos D. Karousis, Ivo Ban, Christopher P. Lapointe, Marc Leibundgut, Emilie Bäumlin, Eric Kummerant, Alain Scaiola, Tanja Schönhut, Jana Ziegelmüller, et al.reaction was mixed with 50 μl 1x Renilla-Glo substrate in Renilla-Glo assay buffer (Promega, Cat. No. E2720) on a white-bottom 96-well plate (Greiner, Cat. No. 655073). The luminescence signal was measured three

(17) SARS-CoV-2 omicron variants are susceptible in vitro to Artemisia annua hot water extractsJournal of EthnopharmacologyMay 23, 2023M.S. Nair, Y. Huang, M. Wang, P.J. Weathersreagents Reagents were procured from Sigma-Aldrich (St. Louis, MO). Renilla-Glo was from Promega (E2720). EMEM (Cat # 30–2003) and XTT reagent (Cat # 30-1011 k) were from ATCC. 2.6 Statistical analyses The

(18) Mitigating the risk of antimalarial resistance via covalent dual-subunit inhibition of the Plasmodium proteasomeCell Chemical BiologyMay 18, 2023Ioanna Deni, Barbara H. Stokes, Kurt E. Ward, Kate J. Fairhurst, Charisse Flerida A. Pasaje, Tomas Yeo, Shirin Akbar, Heekuk Park, Ryan Muir, Daniella S. Bick, et al.were taken at 0, 24, and 72 h post-invasion using the Renilla-Glo(R) Luciferase Assay System (Promega E2750) and the GloMax® Discover Multimode Microplate Reader (Promega). Results were visualized using GraphPad

(19) Potent acyl-CoA synthetase 10 inhibitors kill Plasmodium falciparum by disrupting triglyceride formationNature CommunicationsMarch 16, 2023Selina Bopp, Charisse Flerida A. Pasaje, Robert L. Summers, Pamela Magistrado-Coxen, Kyra A. Schindler, Victoriano Corpas-Lopez, Tomas Yeo, Sachel Mok, Sumanta Dey, Sebastian Smick, et al.72, and 120 h by quantitating luminescence using the Renilla-Glo(R) Luciferase Assay System (ref no E2750, Promega) and the GloMax® Discover Multimode Microplate Reader (Promega). The luminescence values were

(20) Cytoplasmic Isoleucyl tRNA synthetase as an attractive Multi-stage antimalarial drug target*Science Translational MedicineMarch 8, 2023Eva S. Istvan, Francisco Guerra, Matthew Abraham, Kuo-Sen Huang, Frances Rocamora, Haoshuang Zhao, Lan Xu, Charisse Pasaje, Krittikorn Kumpornsin, Madeline R. Luth, et al.aTc. RLuc levels were measured at 0 and 72 h using the Renilla-Glo(R) Luciferase Assay System (Promega E2750) and the GloMax® Discover Multimode Microplate Reader (Promega). Growth was determined by normalizing

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Renilla-Glo Luciferase Assay System

Renilla-Glo Luciferase Assay System from Promega

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Renilla-Glo Luciferase Assay System from Promega

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