GSH/GSSG-Glo Assay V6612 from Promega

Product Specs
ItemGSH/GSSG-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV6612
Quantity50ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Discovery and Preclinical Validation of a Clinically Optimized Mitochondrial Complex I Modulator for Alzheimer’s DiseasebioRxivApril 17, 2026Sergey Trushin, Thi Kim Oanh Nguyen, Mark Ostroot, Alexander Galkin, Toshihiko Nambara, Wenyan Lu, Takahisa Kanekiyo, Graham Johnson, Eugenia Trushina(Promega, G9081), respectively. Glutathione levels were measured using the GSH/GSSG-Glo™ Assay (Promega, V6612). Lactate levels were determined using the Lactate-Glo™ Assay (Promega, J5021). Antioxidant capacity

(2) ATR Blockade Potentiates the Effects of Genotoxic Agents In Vitro and Promotes Antitumor Immunity in a Mouse Model of Non-Small Cell Lung CancerCancers (Basel)March 3, 2026Dimitra Mavroeidi, Christina Papanikolaou, Elisavet Deligianni, Panagiotis Malamos, Panagiota Stamou, Konstantinos N. Syrigos, Vassilis L. Souliotis(GSH/GSSG) was measured using the luminescence-based GSH/GSSG-Glo Assay from Promega (Madison, WI, USA, V6612), following the manufacturer’s instructions, allowing quantification of total glutathione (GSH + GSSG),

(3) Semaglutide Protects Retinal Ganglion Cells Against Rotenone-Induced Degeneration via Improved Glucose MetabolismInvest Ophthalmol Vis SciFebruary 10, 2026Zaynab A. Mouhammad, James R. Tribble, Alan Nicol, Evgenia Andreopoulou, Mariana Y. García-Bermúdez, Blanca I. Aldana, Rupali Vohra, Miriam Kolko, Pete A. Williamsratio were quantified using the GSH/GSSG-Glo Assay (Promega Corporation, Madison, WI, USA; Cat. V6611/V6612). The assay was performed according to the manufacturer's instructions. For each sample, 25 µL of diluted

(4) Glutamine enhances endothelial cell survival and vasodilation by increasing glutathione to reduce oxidative stressPhysiological ReportsJanuary 21, 2026Marzyeh Kheradmand, Gurneet Sangha, Claire M. Sissons, Michael Sun, Xinyao Zhou, Lauren V. Smith, Meagan Bauer, Chengpeng Chen, Alisa Morss Clynenuclei. 2.5 Glutathione Intracellular glutathione was measured using a GSH/GSSG Glo assay (Promega, V6612) and liquid chromatography‐mass spectrometry (LC‐MS). For the Glo assay, HCAEC were seeded in a white

(5) Mitochondrial superoxide dismutase controls metabolic plasticity in pancreatic cancerCell Commun SignalDecember 6, 2025Sankaranarayanan Ramasubramanian, Rupert Öllinger, Carola Eberhagen, Hans Zischka, Roland M. Schmid, Henrik Einwächteroxidized glutathione (GSSG) following the manufacturer’s instructions using the GSH/GSSG-Glo™ Assay kit (V6612, Promega). Results Low Sod2 expression correlates with improved prognosis in pancreatic cancer patients Given

(6) DNA Damage Response and Redox Status in the Resistance of Multiple Myeloma Cells to Genotoxic TreatmentInt J Mol SciOctober 19, 2025Panagiotis Malamos, Christina Papanikolaou, Elisavet Deligianni, Dimitra Mavroeidi, Konstantinos Koutoulogenis, Maria Gavriatopoulou, Evangelos Terpos, Vassilis L. Souliotisluminescence-based assay following the manufacturer’s protocol (GSH/GSSG-Glo Assay, Promega, Madison, WI, USA, #V6612). A total of 1 × 104 cells were used per condition. For the quantification of apurinic/apyrimidinic

(7) A Redundant System of Thioredoxin and Glutathione Is Essential for Pancreatic Acinar IntegrityCellular and Molecular Gastroenterology and HepatologySeptember 2, 2025Henrik Einwächter, Bailing Li, Michaela Aichler, Mariana Rickmann, Nirav Florian Chhabra, Rupert Oellinger, Markus Brielmeier, Roland M. Schmiddrinking water for 24 and 48 hours. GSH and GSSG were assayed using the GSH/GSSG-Glo assay from Promega (# V6612) according to the manufacturer’s protocol. Induction of Glutathione Depletion in Mice by BSO Treatment To

(8) Association of DNA damage response signals and oxidative stress status with nivolumab efficacy in patients with Head and Neck Squamous Cell CarcinomaBritish Journal of CancerAugust 24, 2025Christina Papanikolaou, Panagiota Economopoulou, Aris Spathis, Ioannis Kotsantis, Niki Gavrielatou, Maria Anastasiou, Myrto Moutafi, Anastasios Kyriazoglou, George-Romanos P. Foukas, Ioannis M. Lelegiannis, et al.apurinic/apyrimidinic) sites Oxidative stress was measured using the GSH/GSSG-Glo assay (Promega; #V6612). Abasic sites were quantified using an AP-sites quantitation kit (AP sites; Cell Biolabs; #STA-324).

(9) TFE3 fusions drive oxidative metabolism and ferroptosis resistance in translocation renal cell carcinomaEMBO Molecular MedicineMarch 27, 2025Alexandra Helleux, Guillaume Davidson, Antonin Lallement, Fatima Al Hourani, Alexandre Haller, Isabelle Michel, Anas Fadloun, Christelle Thibault-Carpentier, Xiaoping Su, Véronique Lindner, et al.oxidized glutathione (GSSG) levels were measured in cells using the GSH/GSSG-Glo Assay kit (Promega, V6612) following manufacturer’s instructions. Cells were plated on 96-well plate (60,000 cells/well) and incubated

(10) PRPS activity tunes redox homeostasis in Myc-driven lymphomabioRxiv : the preprint server for biologyFebruary 28, 2025MacMillan AC, Karki B, Yang J, Gertz KR, Zumwalde S, Patel JG, Czyzyk-Krzeska MF, Meller J, Cunningham JT.NAD+/NADH-Glo Assay (Promega #G9072); NADP+/NADPH-Glo Assay (Promega #G9082); GSH/GSSG-Glo Assay (Promega #V6612). All absorbance measurements were obtained via BMG Labtech CLARIOStar microplate reader, and data was

(11) UVC-Induced Oxidative Stress and DNA Damage Repair Status in Head and Neck Squamous Cell Carcinoma Patients with Different Responses to Nivolumab TherapyBiology (Basel)February 13, 2025Christina Papanikolaou, Panagiota Economopoulou, Niki Gavrielatou, Dimitra Mavroeidi, Amanda Psyrri, Vassilis L. SouliotisAP) Sites Oxidative stress was quantified by a luminescence-based assay (Promega, Madison, WI, USA; #V6612), and AP sites were measured using an AP site assay kit (Cell Biolabs, San Diego, CA, USA; #STA-324).

(12) MTHFD2-mediated redox homeostasis promotes gastric cancer progression under hypoxic conditionsRedox ReportDecember 31, 2024Hai-Yu Mo, Ruo-Bing Wang, Meng-Yao Ma, Yi Zhang, Xin-Yu Li, Wang-Rong Wen, Yi Han, Tian Tianlevels of GSH/oxidized glutathione (GSSG) and NADPH/NADP+ were measured using a GSH/GSSG Assay kit (#V6612) and an NADPH/NADP+ Assay kit (#G9081) (Promega, WI) according to the manufacturer’s instructions. 2.10.

(13) DNA Damage Response Network and Intracellular Redox Status in the Clinical Outcome of Patients with Lung CancerCancers (Basel)December 18, 2024Dimitra Mavroeidi, Anastasia Georganta, Dimitra T. Stefanou, Christina Papanikolaou, Konstantinos N. Syrigos, Vassilis L. SouliotisGSH/GSSG ratio, following the manufacturer’s instructions (GSH/GSSG-Glo Assay, Promega, Madison, WI, USA, #V6612). Abasic sites were analyzed with the OxiSelect Oxidative DNA Damage Quantitation Kit (Cell Biolabs,

(14) Deciphering the ferroptosis pathways in dorsal root ganglia of Friedreich ataxia models. The role of LKB1/AMPK, KEAP1, and GSK3β in the impairment of the NRF2 responseRedox biologyOctober 1, 2024Sanz-Alcázar A, Portillo-Carrasquer M, Delaspre F, Pazos-Gil M, Tamarit J, Ros J, Cabiscol E.collagen. To quantify the GSH, GSSG, and total GSH levels, the GSH/GSSG-Glo™ Assay (Promega, reference V6612) was performed according to the manufacturer's instructions. Briefly, on day 5 after lentivirus transduction,

(15) A superoxide-driven redox state promotes geroconversion and resistance to senolysis in replication-stress associated senescenceRedox biologyAugust 1, 2023Luo L, Pervaiz S, Clement MV.measurement of Reduced glutathione (GSH) and oxidized glutathione (GSSG) using the GSH/GSSG-Glo™ Assay (Cat. #V6612; Promega). Experiments were performed following the manufacturer's protocol. 5.10 Nuclear-Cytoplasmic

(16) Inositol polyphosphate multikinase modulates redox signaling through nuclear factor erythroid 2-related factor 2 and glutathione metabolismiScienceJuly 21, 2023Tyagi R, Chakraborty S, Tripathi SJ, Jung IR, Kim SF, Snyder SH, Paul BD.330231" Nuclear –Cytoplasmic fractionation kit,Biovision,K266-25 Promega GSH/GSSG-Glo™ Glutathione Assay,Promega,V6612 Protein Assay Dye Reagent,Biorad,22660 RNeasy Plus Universal Kit,Qiagen,73404 RNeasy Plus Mini Kit (50

(17) Chronological Age and DNA Damage Accumulation in Blood Mononuclear Cells: A Linear Association in Healthy Humans after 50 Years of AgeInternational Journal of Molecular SciencesApril 12, 2023Nikolaos I. Vlachogiannis, Panagiotis A. Ntouros, Maria Pappa, Evrydiki Kravvariti, Evangelia Georgia Kostaki, Georgios E. Fragoulis, Christina Papanikolaou, Dimitra Mavroeidi, Vasiliki-Kalliopi Bournia, Stylianos Panopoulos, et al.ratio (GSH/GSSG) according to the manufacturer’s experimental protocol (GSH/GSSG-Glo™ Assay; Promega, #V6612, Madison, WI, USA). The levels of abasic (apurinic/apyrimidinic; AP) sites were also evaluated using

(18) Lpp of Escherichia coli K1 inhibits host ROS production to counteract neutrophil-mediated eliminationRedox BiologyFebruary 1, 2023Xue-Wei Zhang, Ming-Xin An, Zeng-Kang Huang, Lan Ma, Dan Zhao, Zhao Yang, Jun-Xiu Shi, Dong-Xin Liu, Qiang Li, An-Hua Wu, et al.96-well luminometer-compatible plate followed by measurement with GSH/GSSG-Glo Assay Kit (Promega, V6612) according to manufacturer's instructions. In brief, the Total Glutathione Lysis Reagent were added

(19) Actein contributes to black cohosh extract‐induced genotoxicity in human TK6 cellsJournal of Applied ToxicologySeptember 1, 2022Yuan Le, Xilin Li, Si Chen, Kylie G. Ning, Xiaoqing Guo, Charles G. Wu, Mugimane G. Manjanatha, Nan Meiglutathione and oxidized glutathione (GSH/GSSG) ratios were measured using GSH/GSSG-Glo assay kit (Cat# V6612; Promega) following 4- and 24-h treatments as described previously (Guo et al., 2015). 2.5 | Measurement

(20) Oxidative Stress and Deregulated DNA Damage Response Network in Lung Cancer PatientsBiomedicinesMay 26, 2022Stefanou DT, Kouvela M, Stellas D, Voutetakis K, Papadodima O, Syrigos K, Souliotis VL.triplicate. 2.5. Assessment of Oxidative Stress and Abasic Sites The GSH/GSSG-Glo™ Assay (Promega, #V6612, Wisconsin, USA) was used to measure the reduced glutathione (GSH) to oxidized glutathione (GSSG) ratio

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GSH/GSSG-Glo Assay

GSH/GSSG-Glo Assay from Promega

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GSH/GSSG-Glo Assay from Promega

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