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ApoTox-Glo Triplex Assay
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ApoTox-Glo Triplex Assay
ApoTox-Glo Triplex Assay
ApoTox-Glo Triplex Assay from Promega
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Citations:
(65)
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ApoTox-Glo Triplex Assay
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Description
The ApoTox-Glo Triplex Assay combines three assay chemistries to easily assess viability, cytotoxicity and apoptosis events in the same cell-based assay well
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
ApoTox-Glo Triplex Assay
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G6320
Quantity
10ml
Molecular Weight
~81kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (65)
(1)
Co-targeting cystathionine gamma-lyase and mTOR: a promising therapeutic strategy in clear cell ovarian cancer
BMC Cancer
May 4, 2026
Longyijie Wei, Amal M. EL-Naggar, Yuqin Li, Yuchen Ding, Eunice Li, TszYin Lam, Yemin Wang, Busra Turgu, David G. Huntsman
model.
Apoptosis assay
OVISE cells were quantified for apoptosis using the
ApoTox-Glo Triplex Assay
(#
G6320
;
Promega
). Cells were seeded at 10,000 cells/well in 96-well plates with 100 μL medium per well. After
(2)
Dystrophins DP71 and DP427 determine cell viability during proliferation and myofibre differentiation
Cell Death & Disease
April 11, 2026
Sylwia Szwec, Alicja Durska, Paulina Kościelniak-Wawro, Jeffrey S. Chamberlain, Oleksandr Ostrovskyy, Karolina Dominiak, Solmaz Karimi, Patryk Konieczny
cytotoxicity, and apoptosis were measured in the same well via the
ApoTox-Glo Triplex Assay
(
Promega
,
G6320
) according to the manufacturer’s instructions. Viability and cytotoxicity were assessed with a nonlytic
(3)
Defining the RNA Modification Landscape of Multiple Myeloma Reveals METTL3-Dependent m 6 A Regulation of NEAT1
bioRxiv
April 7, 2026
Prasanth Thunuguntla, Dhanusha Duraiyan, Catheryn Sizemore, Elizabeth Sulvaran-Guel, Richa Mishra, Jessica Camacho, Savannah Gonzales, Stephen Daly, Katelyn Bagwill, Dakota Colbert,
et al.
apoptosis was also measured using the
ApoTox-Glo™ Triplex Assay
(
Promega
, Madison, WI, USA; catalog no.
G6320
), performed according to the manufacturer’s instructions and as previously described.50 Control or NEAT1-modified
(4)
p53 status determines the epigenetic response to demethylating agents azacitidine and decitabine
EMBO Reports
January 7, 2026
Emma Langdale Hands, Arndt Wallmann, Gabrielle Oxley, Sophie Storrar, Rochelle D’Souza, Mathew Van de Pette
,
Thermo Fisher
,
88803
Poly-D-Lysine,Gibco,A3890401
SSC buffer
,
Merck
,
S6639
Kits,Kits,Kits ApoTox-Glo Triplex kit,
Promega
,
G6320
RNeasy Plus Mini kit,Qiagen,24136
QIAshredder
,
Qiagen
,
79656
HS RNA Qubit kit,
Thermo Fisher
,
Q32852
ReverAid
(5)
Modulation of the APOM/S1PR4 Pathway Reduces Podocyte Lipid Overload in Alport Syndrome via Distinct Autophagy and Efflux Mechanisms
J Am Soc Nephrol
December 31, 2025
Matthew Tolerico, Judith Molina, Arianna Insenga, Arianna Carrazco, Rachel Njeim, Alexis Sloan, Anthony Griswold, Makoto Kurano, Armando Mendez, Robin Nathans,
et al.
assays
Apoptosis/cytotoxicity
assays
were performed using the
ApoTox-Glo™ Triplex assay
(
Promega
,
G6320
) according to the manufacturer’s protocol. Values were expressed as the apoptosis/viability ratios and
(6)
Modulation of the Apolipoprotein M/S1PR4 Pathway Reduces Podocyte Lipid Overload in Alport Syndrome via Distinct Autophagy and Efflux Mechanisms
Journal of the American Society of Nephrology
December 31, 2025
Matthew Tolerico, Judith Molina, Arianna Insenga, Arianna Carrazco, Rachel Njeim, Alexis Sloan, Anthony Griswold, Makoto Kurano, Armando Mendez, Robin Nathans,
et al.
assays
Apoptosis/cytotoxicity
assays
were performed using the
ApoTox-Glo™ Triplex assay
(
Promega
,
G6320
) according to the manufacturer’s protocol. Values were expressed as the apoptosis/viability ratios and
(7)
Common and rare variant analyses reveal genetic factors underlying idiopathic pulmonary fibrosis and its shared aetiology with severe COVID-19
eBioMedicine
December 1, 2025
Athanasios Kousathanas, Christopher A. Odhams, James Cook, Yao Hu, Stephan Klee, A. Mesut Erzurumluoglu, Fidel Ramirez, Christoph Mayr, Dennis Schäfer, Lara Beck,
et al.
treated for 24 h with our MCL-1 antagonists in several concentrations. Then, the ApoToxGlo-
Assay
(
G6321
,
Promega
) was performed according to the instruction manual to determine viability, cytotoxicity, and
(8)
Homeobox protein B6 and homeobox protein B8 control immune-cancer cell interactions in pancreatic cancer
Mol Biomed
July 7, 2025
Ludivine Bertonnier-Brouty, Sara Bsharat, Kavya Achanta, Jonas Andersson, Thanya Pranomphon, Tania Singh, Tuomas Kaprio, Jaana Hagström, Caj Haglund, Hanna Seppänen,
et al.
in 96-well plate and incubated for 48 h or 7 days.
ApoTox-Glo triplex assay
kit (
Promega
, WI, USA,
G6320
) was used according to manufacturer's instructions. Senescence Senescence-associated β-galactosidase
(9)
Avid lysosomal acidification in fibroblasts of the Mediterranean mouse Mus spretus
bioRxiv
February 7, 2025
Melissa Sui, Joanne Teh, Kayleigh Annette Fort, Daniel E Shaw, Peter H Sudmant, Tsuyoshi Koide, Jeffrey M. Good, Juan Manuel Vazquez, Rachel B. Brem
were the
Abcam
Ltd.
Senescence Detection Kit
(Cat. #
ab65351
),
ApoTox-Glo™ Triplex Assay
(
Promega
Cat. #
G6321
), and LysoTracker™ Green DND-26 (
Thermo Fisher
Cat. #
L7526
). Primary and secondary antibodies used
in western blots
(10)
Combining Mitomycin C with inhibition of BAD phosphorylation enhances apoptotic cell death in advanced cervical cancer
Translational Oncology
November 1, 2024
Liqiong Wang, Xi Zhang, Shu Chen, Qiuhua Ye, Basappa Basappa, Tao Zhu, Peter E. Lobie, Vijay Pandey
cell-based
assays
performed in media supplemented with 2 % FBS. The
ApoTox-Glo™ Triplex Assay
Kit (
G6320
,
Promega
, China) was used to evaluate cell viability, apoptosis, and cytotoxicity, following the same
(11)
Vitamin D Significantly Inhibits Carcinogenesis in the Mogp-TAg Mouse Model of Fallopian Tube Ovarian Cancer
Nutrients
September 30, 2024
Omar L. Nelson, Rebecca Rosales, Jane Turbov, Larry G Thaete, Gayathriy Balamayooran, J Mark Cline, J. Wesley Pike, Gustavo C. Rodriguez
cytotoxicity, and caspase activity were examined using
ApoTox-Glo Triplex Assay
(
Promega
, Madison, WI, USA, #
G6321
) according to the manufacturer’s instructions using 5000 cells per well on a 96-well white plate in
(12)
Phosphodiesterase Inhibition to Sensitize Non-Small-Cell Lung Cancer to Pemetrexed: A Double-Edged Strategy
Cancers
July 6, 2024
Ivanina Foureau AV, Foureau DM, McHale CC, Guo F, Farhangfar CJ, Mileham KF.
and Drug Interaction Analysis Following manufacturer instructions, the
ApoTox-Glo Triplex assay
(#
G6321
,
Promega
, Madison, WI, USA) was used to determine the effect of either PDEi, PMX, or of the combination
(13)
E2F transcription factors promote tumorigenicity in pancreatic ductal adenocarcinoma
Cancer Medicine
May 1, 2024
Ludivine Bertonnier‐Brouty, Jonas Andersson, Tuomas Kaprio, Jaana Hagström, Sara Bsharat, Olof Asplund, Gad Hatem, Caj Haglund, Hanna Seppänen, Rashmi B. Prasad,
et al.
apoptosis were measured 48 h and 7 days after the knock‐down using
ApoTox‐Glo triplex assay
kit (
Promega
,
G6320
) according to manufacturer's instructions. 2.13 Migration
Cell culture
inserts in 24‐well plate (8
(14)
Influence of Zika virus on the cytotoxicity, cell adhesion, apoptosis and inflammatory markers of glioblastoma cells
Oncology Letters
February 28, 2024
Daniel Rodrigo Marinowic, Gabriele Goulart Zanirati, Pamella Nunes Azevedo, Ângela Zanatta, Ismael Plentz, Allan Marinho Alcará, Fernanda Bueno Morrone, Thamiris Becker Scheffel, Angélica Regina Cappellari, Paulo Michel Roehe,
et al.
triplicate. To quantify the viability/cytotoxicity of infected cells, the
ApoTox-Glo™ Triplex Assay
(cat. no.
G6320
;
Promega Corporation
) was used. Briefly, 20 µl Viability/Cytotoxicity Reagent was added to each well
(15)
Mild Uncoupling of Mitochondria Synergistically Enhances Senolytic Specificity and Sensitivity of BH3 Mimetics
Aging Biology
February 20, 2024
Edward P. Fielder, Edward P. Fielder, Abbas Ishaq, Joseph A. Laws, Joseph A. Calista, Aisha Calista, Thomas von Zglinicki, Thomas von Zglinicki
manually counted using a hemocytometer.
ApoTox-Glo triplex assay
The
ApoTox-Glo triplex assay
(
Promega
,
G6320
) was performed in 96-well plates (Greiner Bio-one #655090) according to the manufacturer’s protocol.
(16)
Fructose regulates the pentose phosphate pathway and induces an inflammatory and resolution phenotype in Kupffer cells
Scientific reports
February 18, 2024
Lodge M, Scheidemantle G, Adams VR, Cottam MA, Richard D, Breuer D, Thompson P, Shrestha K, Liu X, Kennedy A.
were tested for viability, cytotoxicity and apoptotic activity following
ApoTox-Glo Triplex Assay
(
G6320
Promega
) manufacturers protocol. Statistical analysis GraphPad Prism 9.3.1 software was used for all
(17)
Determination of the optical interference of iron oxide nanoparticles in fluorometric cytotoxicity assays
Heliyon
February 15, 2024
Leisha Martin, Kimberly Lopez, Shayden Fritz, Charles P. Easterling, Jacob A. Krawchuck, Agus R. Poerwoprajitno, Wei Xu
purified but were used as received from the manufacturer. The
ApoTox-Glo™ triplex assay
(Catalog No.
G6320
) was purchased from
Promega
® (Madison, WI). Staurosporine was purchased from AAT Bioquest, Inc., (Pleasanton,
(18)
Heat stress suppresses MnSOD expression via p53-Sp1 interaction and induces oxidative stress damage in endothelial cells: Protective effects of MitoQ10 and Pifithrin-α
Heliyon
December 1, 2023
Jian Gong, Peipei Sun, Li Li, Zhimin Zou, Qihua Wu, Liyun Sun, Hui Li, Zhengtao Gu, Lei Su
apoptosis assay
Cell viability and apoptosis were assessed using the
ApoTox-Glo™ Triplex Assay
kit (
Promega
,
G6320
) in 96-well
cell cultures
. Cells were treated with reaction substrate or control carrier (100 μL), incubated
(19)
DoxoDB: A Database for the Expression Analysis of Doxorubicin-Induced lncRNA Genes
Non-Coding RNA
July 13, 2023
Rebecca Distefano, Mirolyuba Ilieva, Jens Hedelund Madsen, Sarah Rennie, Shizuka Uchida
cellular viability, cytotoxicity, and apoptosis,
ApoTox-Glo Triplex Assay
(
Promega
, Nacka, Sweden, #
G6321
) was used according to the manufacturer’s protocol. The fluorescent and luminescent signals were read
(20)
Integrated multi-omics for rapid rare disease diagnosis on a national scale
Nature Medicine
July 1, 2023
Sebastian Lunke, Sophie E. Bouffler, Chirag V. Patel, Sarah A. Sandaradura, Meredith Wilson, Jason Pinner, Matthew F. Hunter, Christopher P. Barnett, Mathew Wallis, Benjamin Kamien,
et al.
cytotoxicity were assessed after heat
shock
, with the
ApoTox-Glo Triplex Assay
kit (
Promega
, cat. no.
G6320
) according to previously described methods with minor modifications22. To expose cells to heat
shock
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ApoTox-Glo Triplex Assay from Promega
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